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The map label for this gene is tilS

Identifier: 46446273

GI number: 46446273

Start: 782621

End: 783997

Strand: Direct

Name: tilS

Synonym: pc0639

Alternate gene names: 46446273

Gene position: 782621-783997 (Clockwise)

Preceding gene: 46446272

Following gene: 46446274

Centisome position: 32.41

GC content: 34.79

Gene sequence:

>1377_bases
ATGTCTTGTATTTGCTTAAAAAATGAGAAACTAAATGTGATTAATCAAGTTAAAAGATTTATCAAAAAGTATTGTAAATC
AACACAGCCACTTCTATTAGCTCTTTCAGGAGGTGCTGACTCTCTTTCTCTTTTTTATTGTTTACTTGCTTGCCGATTAG
AAGGAATTCTCTCTTTTCATGTTGCTCATGTTGATCATGGTTGGCGTGAAGAGAGTGCCAAGGAAGCAACCATATTGAAA
AATTTAGCAGAGCAACACCAAGTTCCTTATCATCAGCTGAAAATCGATCTCTCACAATTAGCTGGAAATTTAGAAGATGC
CTGTCGAAAACAACGGCAGAAATTTTTTAAGGAAATTTGCCTGCAAAATAATTTACAAGCTGTTTGTCTAGGACATCAAG
AAAATGATCAAGTGGAGACTGTTTTAAAAAGAATTTTAGAGGGATCTCACTGGTCTCATTTTGACGGATTAAAAGAGAGA
ATGTGGCACGATCAAGTCCAGTTTTTACGTCCTTTACTAGGAATTCAGAAAGATGAGATTTTAGCTTTTCTGAAAGGAAA
TCAACTCAAAGCGTTTGAAGATGGGACAAATTGTGACGAAAGATTTATGAGAGCTCGCATGCGACGAACTATTATTCCAG
ACTTAAATCGATCCTTTGGGAAGAAAATAGACAACTCATTAGTTTACATTGCATCTGAAATGAGTGAATTAAAAAATTTT
TTTTTAGGCCGAGTGTCTCCTTTACTAGGTCAGATCGTTAAAGGTCCTTTTGGAGTCTATTTAAATTTAGCCAACCATCC
CTCTATAGATCTGGTTGAAATCAAGTATCTTTTACGTTTAATCAGCGAACAAGAATTATTTTTTCTATCTCGACAAATCC
TTCAAAGAGCATCAGAGGCAATTGAAAAGTTGGAGCCCCAACTAAGTTTTGAGATGGGAACGAAAAAAATCGTTATCGAT
CGAGGACATTTTTTTATTCTTTCAAAAAAAACTGCATGTAAGAATCCAAACTTGCAAATTTCTGGCTCTAGCCTTCAGTA
TTATGGGAAATGGATGATAAAAACGAATGAGAGTTTCTATCATCAAAATTTACAAGCTTCAACTTGGTTAGATGGTTGGC
GTGGTTATTTAAAAACATATCTTCCTCTGGATAAGTATGTATTAGGGCAGGCCTCTAAACACGCCAAACTTTTAAGACAT
CACTCCACAATCAATAAATGGTGGAGTTCGCATCACGTTCCTCCCTTTTTACGCTCTTTTTTTCCAGTAATTTGGCAACA
TAATGAAATTGCTCATGAATTTTTAACTGGTTTAGAAAGACAAAATTTGCAAGAAGGAGAAAAATGTTTGCAAATAGAAT
TAAGTTATCTTACATAA

Upstream 100 bases:

>100_bases
TTACCTTCAAAATTGGTTGATGAAGAGGGAACGTTAACTATTCAACAGGATCCTTTTCCATTTTTACTTTGGATTTTGAT
TTTGCTTCTATCTTTAATTT

Downstream 100 bases:

>100_bases
GAAAAATACAACAGCATATCAGAATTTTTGTGATTTCAATGTGAGATGTTGTAGTAAATTAAAAAATATCTTAAGTTGTG
GATTTTGTGGGAATTGGAAT

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MSCICLKNEKLNVINQVKRFIKKYCKSTQPLLLALSGGADSLSLFYCLLACRLEGILSFHVAHVDHGWREESAKEATILK
NLAEQHQVPYHQLKIDLSQLAGNLEDACRKQRQKFFKEICLQNNLQAVCLGHQENDQVETVLKRILEGSHWSHFDGLKER
MWHDQVQFLRPLLGIQKDEILAFLKGNQLKAFEDGTNCDERFMRARMRRTIIPDLNRSFGKKIDNSLVYIASEMSELKNF
FLGRVSPLLGQIVKGPFGVYLNLANHPSIDLVEIKYLLRLISEQELFFLSRQILQRASEAIEKLEPQLSFEMGTKKIVID
RGHFFILSKKTACKNPNLQISGSSLQYYGKWMIKTNESFYHQNLQASTWLDGWRGYLKTYLPLDKYVLGQASKHAKLLRH
HSTINKWWSSHHVPPFLRSFFPVIWQHNEIAHEFLTGLERQNLQEGEKCLQIELSYLT

Sequences:

>Translated_458_residues
MSCICLKNEKLNVINQVKRFIKKYCKSTQPLLLALSGGADSLSLFYCLLACRLEGILSFHVAHVDHGWREESAKEATILK
NLAEQHQVPYHQLKIDLSQLAGNLEDACRKQRQKFFKEICLQNNLQAVCLGHQENDQVETVLKRILEGSHWSHFDGLKER
MWHDQVQFLRPLLGIQKDEILAFLKGNQLKAFEDGTNCDERFMRARMRRTIIPDLNRSFGKKIDNSLVYIASEMSELKNF
FLGRVSPLLGQIVKGPFGVYLNLANHPSIDLVEIKYLLRLISEQELFFLSRQILQRASEAIEKLEPQLSFEMGTKKIVID
RGHFFILSKKTACKNPNLQISGSSLQYYGKWMIKTNESFYHQNLQASTWLDGWRGYLKTYLPLDKYVLGQASKHAKLLRH
HSTINKWWSSHHVPPFLRSFFPVIWQHNEIAHEFLTGLERQNLQEGEKCLQIELSYLT
>Mature_457_residues
SCICLKNEKLNVINQVKRFIKKYCKSTQPLLLALSGGADSLSLFYCLLACRLEGILSFHVAHVDHGWREESAKEATILKN
LAEQHQVPYHQLKIDLSQLAGNLEDACRKQRQKFFKEICLQNNLQAVCLGHQENDQVETVLKRILEGSHWSHFDGLKERM
WHDQVQFLRPLLGIQKDEILAFLKGNQLKAFEDGTNCDERFMRARMRRTIIPDLNRSFGKKIDNSLVYIASEMSELKNFF
LGRVSPLLGQIVKGPFGVYLNLANHPSIDLVEIKYLLRLISEQELFFLSRQILQRASEAIEKLEPQLSFEMGTKKIVIDR
GHFFILSKKTACKNPNLQISGSSLQYYGKWMIKTNESFYHQNLQASTWLDGWRGYLKTYLPLDKYVLGQASKHAKLLRHH
STINKWWSSHHVPPFLRSFFPVIWQHNEIAHEFLTGLERQNLQEGEKCLQIELSYLT

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family

Homologues:

Organism=Escherichia coli, GI1786386, Length=194, Percent_Identity=25.7731958762887, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TILS_PARUW (Q6MDI6)

Other databases:

- EMBL:   BX908798
- RefSeq:   YP_007638.1
- ProteinModelPortal:   Q6MDI6
- STRING:   Q6MDI6
- GeneID:   2780903
- GenomeReviews:   BX908798_GR
- KEGG:   pcu:pc0639
- NMPDR:   fig|264201.1.peg.639
- eggNOG:   COG0037
- ProtClustDB:   CLSK2762207
- BioCyc:   CPRO264201:PC0639-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_01161
- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR014729
- InterPro:   IPR011063
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11807:SF2
- TIGRFAMs:   TIGR02432

Pfam domain/function: PF01171 ATP_bind_3

EC number: NA

Molecular weight: Translated: 53186; Mature: 53055

Theoretical pI: Translated: 9.17; Mature: 9.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSCICLKNEKLNVINQVKRFIKKYCKSTQPLLLALSGGADSLSLFYCLLACRLEGILSFH
CCEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
VAHVDHGWREESAKEATILKNLAEQHQVPYHQLKIDLSQLAGNLEDACRKQRQKFFKEIC
HHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHEEHHHHCCCHHHHHHHHHHHHHHHHH
LQNNLQAVCLGHQENDQVETVLKRILEGSHWSHFDGLKERMWHDQVQFLRPLLGIQKDEI
HHCCCCEEEECCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHH
LAFLKGNQLKAFEDGTNCDERFMRARMRRTIIPDLNRSFGKKIDNSLVYIASEMSELKNF
HHHHCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHHH
FLGRVSPLLGQIVKGPFGVYLNLANHPSIDLVEIKYLLRLISEQELFFLSRQILQRASEA
HHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IEKLEPQLSFEMGTKKIVIDRGHFFILSKKTACKNPNLQISGSSLQYYGKWMIKTNESFY
HHHHCCHHHHHCCCEEEEEECCCEEEEECCCCCCCCCEEEECCCCEEEEEEEEEECCHHH
HQNLQASTWLDGWRGYLKTYLPLDKYVLGQASKHAKLLRHHSTINKWWSSHHVPPFLRSF
HCCCCHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
FPVIWQHNEIAHEFLTGLERQNLQEGEKCLQIELSYLT
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHEEHEEHHCC
>Mature Secondary Structure 
SCICLKNEKLNVINQVKRFIKKYCKSTQPLLLALSGGADSLSLFYCLLACRLEGILSFH
CEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
VAHVDHGWREESAKEATILKNLAEQHQVPYHQLKIDLSQLAGNLEDACRKQRQKFFKEIC
HHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHEEHHHHCCCHHHHHHHHHHHHHHHHH
LQNNLQAVCLGHQENDQVETVLKRILEGSHWSHFDGLKERMWHDQVQFLRPLLGIQKDEI
HHCCCCEEEECCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHH
LAFLKGNQLKAFEDGTNCDERFMRARMRRTIIPDLNRSFGKKIDNSLVYIASEMSELKNF
HHHHCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHHH
FLGRVSPLLGQIVKGPFGVYLNLANHPSIDLVEIKYLLRLISEQELFFLSRQILQRASEA
HHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IEKLEPQLSFEMGTKKIVIDRGHFFILSKKTACKNPNLQISGSSLQYYGKWMIKTNESFY
HHHHCCHHHHHCCCEEEEEECCCEEEEECCCCCCCCCEEEECCCCEEEEEEEEEECCHHH
HQNLQASTWLDGWRGYLKTYLPLDKYVLGQASKHAKLLRHHSTINKWWSSHHVPPFLRSF
HCCCCHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
FPVIWQHNEIAHEFLTGLERQNLQEGEKCLQIELSYLT
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHEEHEEHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA