BacMap

Definition	Candidatus Protochlamydia amoebophila UWE25, complete genome.
Accession	NC_005861
Length	2,414,465

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The map label for this gene is tuf

Identifier: 46446229

GI number: 46446229

Start: 732977

End: 734161

Strand: Direct

Name: tuf

Synonym: pc0595

Alternate gene names: 46446229

Gene position: 732977-734161 (Clockwise)

Preceding gene: 46446228

Following gene: 46446230

Centisome position: 30.36

GC content: 43.63

Gene sequence:

>1185_bases
ATGGCTAAAGAAACTTTTCAGCGGAAGAAGCCACACGTCAACGTTGGAACGATTGGTCACGTCGACCACGGAAAAACCAC
GTTAACAGCCGCTATCACAAAGGTGCTCGCTGAAGCGGGTGGGGCGAAATTTAGAGATTATGCTTCTATTGATAATACGC
CTGAGGAAAAAGCACGTGGTATCACTATTAACTCAAGCCACGTTGAATACGAAACAGATGCACGTCACTATGCACACGTA
GATTGCCCAGGTCACGCCGACTATGTAAAGAACATGATTACCGGTGCTGCTCAGATGGATGGAGCAATCCTCGTTGTTGC
TGCTACAGACGGAGCAATGCCTCAAACGCGCGAACACATTTTGTTAGCTCGCCAAATGCAAGTTCCTGCTATTGTTGTAT
TCCTCAACAAAGTGGACATGCTCGGTGAAAGCGATTCAGAACTTCTCGATCTCGTTGAGATGGAACTACATGAATTGCTC
GAATCTAAAGGTTACCACAATTCTCCAATTATTCGCGGTTCAGCTTTACGTGCATTGGAAGGAGATCCAAAATACGTTGA
AAGTATTAAACAATTGATGAAAATTGTTGATGAAGCTGTTCCAACACCTCAACGTGAGACAGATAAGCCTTTCTTAATGC
CTGTTGAAGACGTCTTTTCTATTTCTGGTCGAGGTACTGTGGCGACAGGTCGTGTAGAGCGTGGTGTTGTTAAGATCAAC
GACAAATTGCAACTCATTGGTCTTGGAGACACACGGGATACAGTAGCCACTGGTCTTGAAATGTTTAATAAAATCTTGGA
AGAAGCTCGCGCAGGAGAAAACGTTGGTATTCTGTTAAGAGGTTTAACTAAGACTGATATTGAGCGTGGAATGTGTCTCG
TTGCTCCTGGAACTTGTACTCCGCATACAGAATTTAAAGGTCCAGTTTACGTATTAACAAAAGAAGAAGGTGGACGTCAT
AAACCATTTTTCTCAGGATATCGTCCTCAGTTATATTTCCGTACTACAGACGTAACTGGAACAATTGAGCTTCCAGCTGG
TGTAGAAATGGTTATGCCTGGCGATAACGTAGAAATTACAGTGAAATTGATTGCTCCAATCGCGATGGAAAAAGGGATGC
GTTTTGCGATTCGTGAAGGTGGTCGTACAATTGGTGCGGGAACCGTTTCCGAAATCCTTAAATAA

Upstream 100 bases:

>100_bases
ATGGTAAGCAAAAGGTCGTAGGTTCAATTCCTATTCTGGGCAGACTTAACATTAATAAACAAGATATATCCATTTACTGG
AGCTTAACGGAGGAATTAAA

Downstream 100 bases:

>100_bases
TCAATTTTTGGTAAAAAGATAAGGGTAGAGTTTTCTCTACCCTTTTTAATAATGTTTTTTGGGTGTGTAGCTCAGCTGGT
AGAGCAACGGTCTCCAAAGC

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MAKETFQRKKPHVNVGTIGHVDHGKTTLTAAITKVLAEAGGAKFRDYASIDNTPEEKARGITINSSHVEYETDARHYAHV
DCPGHADYVKNMITGAAQMDGAILVVAATDGAMPQTREHILLARQMQVPAIVVFLNKVDMLGESDSELLDLVEMELHELL
ESKGYHNSPIIRGSALRALEGDPKYVESIKQLMKIVDEAVPTPQRETDKPFLMPVEDVFSISGRGTVATGRVERGVVKIN
DKLQLIGLGDTRDTVATGLEMFNKILEEARAGENVGILLRGLTKTDIERGMCLVAPGTCTPHTEFKGPVYVLTKEEGGRH
KPFFSGYRPQLYFRTTDVTGTIELPAGVEMVMPGDNVEITVKLIAPIAMEKGMRFAIREGGRTIGAGTVSEILK

Sequences:

>Translated_394_residues
MAKETFQRKKPHVNVGTIGHVDHGKTTLTAAITKVLAEAGGAKFRDYASIDNTPEEKARGITINSSHVEYETDARHYAHV
DCPGHADYVKNMITGAAQMDGAILVVAATDGAMPQTREHILLARQMQVPAIVVFLNKVDMLGESDSELLDLVEMELHELL
ESKGYHNSPIIRGSALRALEGDPKYVESIKQLMKIVDEAVPTPQRETDKPFLMPVEDVFSISGRGTVATGRVERGVVKIN
DKLQLIGLGDTRDTVATGLEMFNKILEEARAGENVGILLRGLTKTDIERGMCLVAPGTCTPHTEFKGPVYVLTKEEGGRH
KPFFSGYRPQLYFRTTDVTGTIELPAGVEMVMPGDNVEITVKLIAPIAMEKGMRFAIREGGRTIGAGTVSEILK
>Mature_393_residues
AKETFQRKKPHVNVGTIGHVDHGKTTLTAAITKVLAEAGGAKFRDYASIDNTPEEKARGITINSSHVEYETDARHYAHVD
CPGHADYVKNMITGAAQMDGAILVVAATDGAMPQTREHILLARQMQVPAIVVFLNKVDMLGESDSELLDLVEMELHELLE
SKGYHNSPIIRGSALRALEGDPKYVESIKQLMKIVDEAVPTPQRETDKPFLMPVEDVFSISGRGTVATGRVERGVVKIND
KLQLIGLGDTRDTVATGLEMFNKILEEARAGENVGILLRGLTKTDIERGMCLVAPGTCTPHTEFKGPVYVLTKEEGGRHK
PFFSGYRPQLYFRTTDVTGTIELPAGVEMVMPGDNVEITVKLIAPIAMEKGMRFAIREGGRTIGAGTVSEILK

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily

Homologues:

Organism=Homo sapiens, GI34147630, Length=396, Percent_Identity=57.3232323232323, Blast_Score=463, Evalue=1e-130,
Organism=Homo sapiens, GI223555963, Length=440, Percent_Identity=29.0909090909091, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI4503475, Length=448, Percent_Identity=28.125, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI5729864, Length=440, Percent_Identity=29.0909090909091, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI4503471, Length=328, Percent_Identity=32.3170731707317, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI54607086, Length=346, Percent_Identity=27.1676300578035, Blast_Score=129, Evalue=7e-30,
Organism=Homo sapiens, GI194018522, Length=440, Percent_Identity=25.6818181818182, Blast_Score=109, Evalue=4e-24,
Organism=Homo sapiens, GI194097354, Length=440, Percent_Identity=25.6818181818182, Blast_Score=108, Evalue=8e-24,
Organism=Homo sapiens, GI194018520, Length=440, Percent_Identity=25.6818181818182, Blast_Score=108, Evalue=8e-24,
Organism=Homo sapiens, GI46094014, Length=442, Percent_Identity=25.5656108597285, Blast_Score=105, Evalue=9e-23,
Organism=Homo sapiens, GI4503507, Length=279, Percent_Identity=26.1648745519713, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI82546879, Length=433, Percent_Identity=24.2494226327945, Blast_Score=89, Evalue=8e-18,
Organism=Homo sapiens, GI94966754, Length=137, Percent_Identity=34.3065693430657, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI157426893, Length=287, Percent_Identity=25.4355400696864, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI45593140, Length=444, Percent_Identity=21.3963963963964, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI18390331, Length=167, Percent_Identity=31.1377245508982, Blast_Score=66, Evalue=6e-11,
Organism=Escherichia coli, GI1790412, Length=395, Percent_Identity=67.3417721518987, Blast_Score=538, Evalue=1e-154,
Organism=Escherichia coli, GI1789737, Length=395, Percent_Identity=67.3417721518987, Blast_Score=538, Evalue=1e-154,
Organism=Escherichia coli, GI2367247, Length=334, Percent_Identity=31.7365269461078, Blast_Score=129, Evalue=5e-31,
Organism=Escherichia coli, GI48994988, Length=291, Percent_Identity=32.6460481099656, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1789108, Length=329, Percent_Identity=28.8753799392097, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1788922, Length=134, Percent_Identity=33.5820895522388, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790835, Length=145, Percent_Identity=32.4137931034483, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1789738, Length=155, Percent_Identity=29.6774193548387, Blast_Score=64, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17556456, Length=401, Percent_Identity=50.6234413965087, Blast_Score=384, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI25141371, Length=387, Percent_Identity=44.9612403100775, Blast_Score=332, Evalue=3e-91,
Organism=Caenorhabditis elegans, GI17552884, Length=328, Percent_Identity=32.9268292682927, Blast_Score=154, Evalue=9e-38,
Organism=Caenorhabditis elegans, GI17569207, Length=328, Percent_Identity=32.9268292682927, Blast_Score=154, Evalue=9e-38,
Organism=Caenorhabditis elegans, GI32566303, Length=299, Percent_Identity=32.7759197324415, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI115532067, Length=441, Percent_Identity=27.891156462585, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI115532065, Length=438, Percent_Identity=27.8538812785388, Blast_Score=124, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI17506081, Length=273, Percent_Identity=30.4029304029304, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI32566629, Length=438, Percent_Identity=25.7990867579909, Blast_Score=108, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI32566301, Length=147, Percent_Identity=36.734693877551, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17557151, Length=231, Percent_Identity=30.7359307359307, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17509919, Length=277, Percent_Identity=25.9927797833935, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17556745, Length=148, Percent_Identity=31.0810810810811, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6324761, Length=398, Percent_Identity=60.8040201005025, Blast_Score=484, Evalue=1e-137,
Organism=Saccharomyces cerevisiae, GI6325337, Length=323, Percent_Identity=33.4365325077399, Blast_Score=152, Evalue=9e-38,
Organism=Saccharomyces cerevisiae, GI6319594, Length=323, Percent_Identity=33.4365325077399, Blast_Score=152, Evalue=9e-38,
Organism=Saccharomyces cerevisiae, GI6322937, Length=457, Percent_Identity=24.0700218818381, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6320377, Length=340, Percent_Identity=27.6470588235294, Blast_Score=87, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6320863, Length=280, Percent_Identity=25.3571428571429, Blast_Score=81, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6323098, Length=141, Percent_Identity=34.0425531914894, Blast_Score=70, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6323320, Length=238, Percent_Identity=28.1512605042017, Blast_Score=68, Evalue=3e-12,
Organism=Drosophila melanogaster, GI281363316, Length=395, Percent_Identity=55.9493670886076, Blast_Score=453, Evalue=1e-127,
Organism=Drosophila melanogaster, GI17864358, Length=395, Percent_Identity=55.9493670886076, Blast_Score=453, Evalue=1e-127,
Organism=Drosophila melanogaster, GI19921738, Length=390, Percent_Identity=49.2307692307692, Blast_Score=390, Evalue=1e-108,
Organism=Drosophila melanogaster, GI45553807, Length=446, Percent_Identity=27.1300448430493, Blast_Score=146, Evalue=3e-35,
Organism=Drosophila melanogaster, GI45553816, Length=446, Percent_Identity=27.1300448430493, Blast_Score=146, Evalue=3e-35,
Organism=Drosophila melanogaster, GI24651721, Length=446, Percent_Identity=27.1300448430493, Blast_Score=146, Evalue=3e-35,
Organism=Drosophila melanogaster, GI17864154, Length=446, Percent_Identity=27.1300448430493, Blast_Score=146, Evalue=3e-35,
Organism=Drosophila melanogaster, GI24652838, Length=446, Percent_Identity=27.1300448430493, Blast_Score=145, Evalue=7e-35,
Organism=Drosophila melanogaster, GI17137572, Length=446, Percent_Identity=27.1300448430493, Blast_Score=145, Evalue=7e-35,
Organism=Drosophila melanogaster, GI19922690, Length=301, Percent_Identity=31.5614617940199, Blast_Score=139, Evalue=4e-33,
Organism=Drosophila melanogaster, GI45550900, Length=437, Percent_Identity=28.8329519450801, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI17137380, Length=325, Percent_Identity=30.1538461538462, Blast_Score=118, Evalue=6e-27,
Organism=Drosophila melanogaster, GI24647048, Length=271, Percent_Identity=25.4612546125461, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24647046, Length=271, Percent_Identity=25.4612546125461, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI78706572, Length=243, Percent_Identity=29.2181069958848, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19922512, Length=427, Percent_Identity=21.7798594847775, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24654852, Length=427, Percent_Identity=21.7798594847775, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI28572034, Length=140, Percent_Identity=41.4285714285714, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24582462, Length=167, Percent_Identity=32.9341317365269, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI28574573, Length=149, Percent_Identity=34.2281879194631, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): EFTU_PARUW (Q6MDN0)

Other databases:

- EMBL:   BX908798
- RefSeq:   YP_007594.1
- HSSP:   P49410
- ProteinModelPortal:   Q6MDN0
- SMR:   Q6MDN0
- STRING:   Q6MDN0
- GeneID:   2780753
- GenomeReviews:   BX908798_GR
- KEGG:   pcu:pc0595
- NMPDR:   fig|264201.1.peg.595
- eggNOG:   COG0050
- HOGENOM:   HBG307581
- OMA:   FEGQVYV
- ProtClustDB:   PRK12735
- BioCyc:   CPRO264201:PC0595-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00118_B
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000
- PANTHER:   PTHR23115:SF31
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00485
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3; SSF50465 Elong_init_C; SSF50447 Translat_factor

EC number: 3.6.5.3

Molecular weight: Translated: 42989; Mature: 42858

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKETFQRKKPHVNVGTIGHVDHGKTTLTAAITKVLAEAGGAKFRDYASIDNTPEEKARG
CCCHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCC
ITINSSHVEYETDARHYAHVDCPGHADYVKNMITGAAQMDGAILVVAATDGAMPQTREHI
EEEECCCEEEECCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHH
LLARQMQVPAIVVFLNKVDMLGESDSELLDLVEMELHELLESKGYHNSPIIRGSALRALE
HHHHHCCCCEEEEEEHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEECCCHHHHCC
GDPKYVESIKQLMKIVDEAVPTPQRETDKPFLMPVEDVFSISGRGTVATGRVERGVVKIN
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHCCCCCCEEEECCHHCCEEEEC
DKLQLIGLGDTRDTVATGLEMFNKILEEARAGENVGILLRGLTKTDIERGMCLVAPGTCT
CEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHCCEEEEECCCCC
PHTEFKGPVYVLTKEEGGRHKPFFSGYRPQLYFRTTDVTGTIELPAGVEMVMPGDNVEIT
CCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEEECCEEEEECCCCEEEEECCCCCEEE
VKLIAPIAMEKGMRFAIREGGRTIGAGTVSEILK
EEEEEHHHHHCCCEEEECCCCCEECCCHHHHHHC
>Mature Secondary Structure 
AKETFQRKKPHVNVGTIGHVDHGKTTLTAAITKVLAEAGGAKFRDYASIDNTPEEKARG
CCHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCC
ITINSSHVEYETDARHYAHVDCPGHADYVKNMITGAAQMDGAILVVAATDGAMPQTREHI
EEEECCCEEEECCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHH
LLARQMQVPAIVVFLNKVDMLGESDSELLDLVEMELHELLESKGYHNSPIIRGSALRALE
HHHHHCCCCEEEEEEHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEECCCHHHHCC
GDPKYVESIKQLMKIVDEAVPTPQRETDKPFLMPVEDVFSISGRGTVATGRVERGVVKIN
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHCCCCCCEEEECCHHCCEEEEC
DKLQLIGLGDTRDTVATGLEMFNKILEEARAGENVGILLRGLTKTDIERGMCLVAPGTCT
CEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHCCEEEEECCCCC
PHTEFKGPVYVLTKEEGGRHKPFFSGYRPQLYFRTTDVTGTIELPAGVEMVMPGDNVEIT
CCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEEECCEEEEECCCCEEEEECCCCCEEE
VKLIAPIAMEKGMRFAIREGGRTIGAGTVSEILK
EEEEEHHHHHCCCEEEECCCCCEECCCHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA