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The map label for this gene is def [H]

Identifier: 46446175

GI number: 46446175

Start: 673720

End: 674190

Strand: Reverse

Name: def [H]

Synonym: pc0541

Alternate gene names: 46446175

Gene position: 674190-673720 (Counterclockwise)

Preceding gene: 46446178

Following gene: 46446174

Centisome position: 27.92

GC content: 35.88

Gene sequence:

>471_bases
GTGAATCATATCGATGATGCATTGAAACAATTAATCTATGATATGGTGGAAACAATGCACACGATGAAAGGGATTGGCTT
AGCAGCCCCTCAAGTTCATCACTCAATTTCATTATTTGTAACATGTGTTCCAGTTAAAAACAAAAATGGTTTATGGGAAT
CAGGGAAAGATCGTGTGTTTATTAACCCTCAAATTCTTAGCATGAGTGAAGAAACGCAAACCTTTTCAGAGGGATGCTTG
TCCATCCCCAATCTCCATATGAATGTGACAAGGCCTGCTAAAATTACGGTTCAAGCTGTTGATTTAGAAGGTAATTTATT
CGAAGAAATTTTCACAGGACTTCAAGCCACTAACTTTATGCATGAATATGATCATCTTAATGGAATCCTGATTATTGATT
ATTATAGTCTGGAAGAGCGAAAATCTTTAGAACAACTTTTTAGCACCTCTATCCAATCGGAGAAATACTAA

Upstream 100 bases:

>100_bases
CTTTTGTTAAAATTGAATTTGTATTAACCAAAATTTAATTATTATGGAATTATCTTTAGCTTATTATAATGATCCCATTT
TAAGGAAAAAAACTGAACGT

Downstream 100 bases:

>100_bases
ATGGAGTGCTTATATCTTTGCTGTAGTGGAAATTTATATGAAAGCTGTTGAAAAATTTTACATAAAGGAAAATTAGCGCA
AAATGCGCTAATTTTGATGC

Product: putative peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase [H]

Number of amino acids: Translated: 156; Mature: 156

Protein sequence:

>156_residues
MNHIDDALKQLIYDMVETMHTMKGIGLAAPQVHHSISLFVTCVPVKNKNGLWESGKDRVFINPQILSMSEETQTFSEGCL
SIPNLHMNVTRPAKITVQAVDLEGNLFEEIFTGLQATNFMHEYDHLNGILIIDYYSLEERKSLEQLFSTSIQSEKY

Sequences:

>Translated_156_residues
MNHIDDALKQLIYDMVETMHTMKGIGLAAPQVHHSISLFVTCVPVKNKNGLWESGKDRVFINPQILSMSEETQTFSEGCL
SIPNLHMNVTRPAKITVQAVDLEGNLFEEIFTGLQATNFMHEYDHLNGILIIDYYSLEERKSLEQLFSTSIQSEKY
>Mature_156_residues
MNHIDDALKQLIYDMVETMHTMKGIGLAAPQVHHSISLFVTCVPVKNKNGLWESGKDRVFINPQILSMSEETQTFSEGCL
SIPNLHMNVTRPAKITVQAVDLEGNLFEEIFTGLQATNFMHEYDHLNGILIIDYYSLEERKSLEQLFSTSIQSEKY

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Escherichia coli, GI1789682, Length=136, Percent_Identity=38.2352941176471, Blast_Score=85, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 17764; Mature: 17764

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHIDDALKQLIYDMVETMHTMKGIGLAAPQVHHSISLFVTCVPVKNKNGLWESGKDRVF
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCEEEEEEEEEECCCCCCCCCCCCEEE
INPQILSMSEETQTFSEGCLSIPNLHMNVTRPAKITVQAVDLEGNLFEEIFTGLQATNFM
ECHHEECCCHHHHHHHHHCCCCCCCEEEECCCCEEEEEEEECCCCHHHHHHHHHHHHHHH
HEYDHLNGILIIDYYSLEERKSLEQLFSTSIQSEKY
HHHHCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNHIDDALKQLIYDMVETMHTMKGIGLAAPQVHHSISLFVTCVPVKNKNGLWESGKDRVF
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCEEEEEEEEEECCCCCCCCCCCCEEE
INPQILSMSEETQTFSEGCLSIPNLHMNVTRPAKITVQAVDLEGNLFEEIFTGLQATNFM
ECHHEECCCHHHHHHHHHCCCCCCCEEEECCCCEEEEEEEECCCCHHHHHHHHHHHHHHH
HEYDHLNGILIIDYYSLEERKSLEQLFSTSIQSEKY
HHHHCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA