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The map label for this gene is fmt

Identifier: 46446038

GI number: 46446038

Start: 541335

End: 542291

Strand: Direct

Name: fmt

Synonym: pc0404

Alternate gene names: 46446038

Gene position: 541335-542291 (Clockwise)

Preceding gene: 46446037

Following gene: 46446042

Centisome position: 22.42

GC content: 38.14

Gene sequence:

>957_bases
GTGAGAAATAGAAAAATGAAGGTTATTTTTTTTGGTACTCCTCTTTTTGCTGCGCAAGTTCTAGAATTCCTTTTGCAAAA
TCAGGTGGAAGTGGTCGCTGTTATTTCCAAACCTGATCGACCAAAAGGGCGTTCAAGCATACCTGTTCCTACTCCAGTTA
AATTAATTGCTCAAAGCTATCACTTACCCCTTTATCAACCTGAAGTAGTTTCATCTTTAGATTTCGCGCCTGTTTTAAAA
AATTATGAAGCAGATTTATTTGTCGTTGTTGCTTATGGAGAAATTATTAAACAACATTTGTTAGACATGCCTAAACGGGC
TTGCATTAATTTACACGCTAGCTTATTGCCAAAATATCGAGGTGCTGCTCCTATTCAACGCTCGATTATTGAAGGAGAAA
AGGAAACAGGCGTAACGATCATGCATATGGTGAAAAAAATGGATGCAGGTGACATGATCAAAAAAGTGTCAGTGCAAATC
ACTTCGGAAATGACTTATGGTGAGCTCGAACAAGCTCTTTGCCAAATAGGAAAGCATGCCTTGCTAGAAGTAATCAAGCA
GTTCGATCGGGGTGAGCCATCTCGTCAAATTCAAGATTCTCATTTAGCAACATTCGCACCAAAGATTGAACTAGAGGATT
GCGAGTTAGATTGGAACCAATCAGCTCAACATTTACACGATTTGGTGAGAGGAGTTAATCCTTATCCTGGAGCTTGGTGT
TATGTCAAAGTTAATGGAGAGCAAAAACGATTAAAAATCAGCCGTACGCGAGTAATCCCTTATCCATCAAACTGTCCTGG
TACTATTTTAGATAGTTCTAAGGGGAATTTGAAAATTTTGACTGGCGATCAAGCATTAGAACTTGTGGAGGTTCAACTGG
AAGGAAAAAAAACAATGACTTCTGAACAATGGATCCGAGGAATGTCAAAAAATCAGTTAAAATTCTTAGTTAATTAA

Upstream 100 bases:

>100_bases
ACTTTTGTCGTTTGACAAAAAGGGGTTTAATGTGCCTTCAGGCGGAAGAAGAGGGATTGAATTTGGAAGAACCCACTGAG
CCTCTAAATGCAAGCAAGTA

Downstream 100 bases:

>100_bases
TATAAGTGAGTTAATAATATAATTTATTTTTATATTATTTTTTTTGATTATTAATTAATGTAAGTTGCAAATAACTAATT
TATTAAAAAATTTGTTGCAG

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MRNRKMKVIFFGTPLFAAQVLEFLLQNQVEVVAVISKPDRPKGRSSIPVPTPVKLIAQSYHLPLYQPEVVSSLDFAPVLK
NYEADLFVVVAYGEIIKQHLLDMPKRACINLHASLLPKYRGAAPIQRSIIEGEKETGVTIMHMVKKMDAGDMIKKVSVQI
TSEMTYGELEQALCQIGKHALLEVIKQFDRGEPSRQIQDSHLATFAPKIELEDCELDWNQSAQHLHDLVRGVNPYPGAWC
YVKVNGEQKRLKISRTRVIPYPSNCPGTILDSSKGNLKILTGDQALELVEVQLEGKKTMTSEQWIRGMSKNQLKFLVN

Sequences:

>Translated_318_residues
MRNRKMKVIFFGTPLFAAQVLEFLLQNQVEVVAVISKPDRPKGRSSIPVPTPVKLIAQSYHLPLYQPEVVSSLDFAPVLK
NYEADLFVVVAYGEIIKQHLLDMPKRACINLHASLLPKYRGAAPIQRSIIEGEKETGVTIMHMVKKMDAGDMIKKVSVQI
TSEMTYGELEQALCQIGKHALLEVIKQFDRGEPSRQIQDSHLATFAPKIELEDCELDWNQSAQHLHDLVRGVNPYPGAWC
YVKVNGEQKRLKISRTRVIPYPSNCPGTILDSSKGNLKILTGDQALELVEVQLEGKKTMTSEQWIRGMSKNQLKFLVN
>Mature_318_residues
MRNRKMKVIFFGTPLFAAQVLEFLLQNQVEVVAVISKPDRPKGRSSIPVPTPVKLIAQSYHLPLYQPEVVSSLDFAPVLK
NYEADLFVVVAYGEIIKQHLLDMPKRACINLHASLLPKYRGAAPIQRSIIEGEKETGVTIMHMVKKMDAGDMIKKVSVQI
TSEMTYGELEQALCQIGKHALLEVIKQFDRGEPSRQIQDSHLATFAPKIELEDCELDWNQSAQHLHDLVRGVNPYPGAWC
YVKVNGEQKRLKISRTRVIPYPSNCPGTILDSSKGNLKILTGDQALELVEVQLEGKKTMTSEQWIRGMSKNQLKFLVN

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family

Homologues:

Organism=Homo sapiens, GI164663775, Length=240, Percent_Identity=30.8333333333333, Blast_Score=102, Evalue=4e-22,
Organism=Homo sapiens, GI21614513, Length=331, Percent_Identity=25.0755287009063, Blast_Score=102, Evalue=7e-22,
Organism=Homo sapiens, GI238814322, Length=314, Percent_Identity=26.1146496815287, Blast_Score=101, Evalue=1e-21,
Organism=Escherichia coli, GI1789683, Length=301, Percent_Identity=40.8637873754153, Blast_Score=245, Evalue=2e-66,
Organism=Escherichia coli, GI1788589, Length=300, Percent_Identity=30.6666666666667, Blast_Score=134, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI133930964, Length=330, Percent_Identity=24.2424242424242, Blast_Score=98, Evalue=5e-21,
Organism=Drosophila melanogaster, GI45550868, Length=311, Percent_Identity=28.6173633440514, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI28571984, Length=233, Percent_Identity=30.4721030042918, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24585660, Length=315, Percent_Identity=22.8571428571429, Blast_Score=75, Evalue=6e-14,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): FMT_PARUW (Q6ME71)

Other databases:

- EMBL:   BX908798
- RefSeq:   YP_007403.1
- ProteinModelPortal:   Q6ME71
- SMR:   Q6ME71
- STRING:   Q6ME71
- GeneID:   2780498
- GenomeReviews:   BX908798_GR
- KEGG:   pcu:pc0404
- NMPDR:   fig|264201.1.peg.404
- eggNOG:   COG0223
- HOGENOM:   HBG571560
- OMA:   IMQMDEG
- PhylomeDB:   Q6ME71
- ProtClustDB:   CLSK2762166
- BioCyc:   CPRO264201:PC0404-MONOMER
- HAMAP:   MF_00182
- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR015518
- Gene3D:   G3DSA:3.10.25.10
- Gene3D:   G3DSA:3.40.50.170
- PANTHER:   PTHR11138
- TIGRFAMs:   TIGR00460

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N; SSF50486 FMT_C_like; SSF53328 formyl_transf

EC number: =2.1.2.9

Molecular weight: Translated: 35830; Mature: 35830

Theoretical pI: Translated: 9.09; Mature: 9.09

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNRKMKVIFFGTPLFAAQVLEFLLQNQVEVVAVISKPDRPKGRSSIPVPTPVKLIAQSY
CCCCEEEEEEECCHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHC
HLPLYQPEVVSSLDFAPVLKNYEADLFVVVAYGEIIKQHLLDMPKRACINLHASLLPKYR
CCCCCCHHHHHCCCHHHHHHCCCCCEEEEEHHHHHHHHHHHCCCHHHHHHHHHHHCCHHC
GAAPIQRSIIEGEKETGVTIMHMVKKMDAGDMIKKVSVQITSEMTYGELEQALCQIGKHA
CCCHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHH
LLEVIKQFDRGEPSRQIQDSHLATFAPKIELEDCELDWNQSAQHLHDLVRGVNPYPGAWC
HHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHCCCCCCCEEE
YVKVNGEQKRLKISRTRVIPYPSNCPGTILDSSKGNLKILTGDQALELVEVQLEGKKTMT
EEEECCCCCEEEEEEEEEECCCCCCCCCEEECCCCCEEEEECCCCEEEEEEEECCCCCCC
SEQWIRGMSKNQLKFLVN
HHHHHCCCCCCCEEEECC
>Mature Secondary Structure
MRNRKMKVIFFGTPLFAAQVLEFLLQNQVEVVAVISKPDRPKGRSSIPVPTPVKLIAQSY
CCCCEEEEEEECCHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHC
HLPLYQPEVVSSLDFAPVLKNYEADLFVVVAYGEIIKQHLLDMPKRACINLHASLLPKYR
CCCCCCHHHHHCCCHHHHHHCCCCCEEEEEHHHHHHHHHHHCCCHHHHHHHHHHHCCHHC
GAAPIQRSIIEGEKETGVTIMHMVKKMDAGDMIKKVSVQITSEMTYGELEQALCQIGKHA
CCCHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHH
LLEVIKQFDRGEPSRQIQDSHLATFAPKIELEDCELDWNQSAQHLHDLVRGVNPYPGAWC
HHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHCCCCCCCEEE
YVKVNGEQKRLKISRTRVIPYPSNCPGTILDSSKGNLKILTGDQALELVEVQLEGKKTMT
EEEECCCCCEEEEEEEEEECCCCCCCCCEEECCCCCEEEEECCCCEEEEEEEECCCCCCC
SEQWIRGMSKNQLKFLVN
HHHHHCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA