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The map label for this gene is ada

Identifier: 45659219

GI number: 45659219

Start: 4171728

End: 4172558

Strand: Reverse

Name: ada

Synonym: LIC13402

Alternate gene names: 45659219

Gene position: 4172558-4171728 (Counterclockwise)

Preceding gene: 45659220

Following gene: 45659218

Centisome position: 97.55

GC content: 35.02

Gene sequence:

>831_bases
ATGAGCCACTATCAAAAAATTGCGGAAGCAATTCAATTCATACAAAAAAATGCGATTTCTCAACCCGAGTTGGACGAGGT
TGCAAAATCAGTAAACTTAAGTCCGTTTCATTTTCAAAGACTGTTTACGGAATGGGCAGGAGTGAGTCCGAAACAATTTC
TACAGTATATTACTTTACAAAATGCTAAATCGATTCTCTCTAAACCTCAAACCACTTTATTTGATGCTGCTTTTGAAACT
GGATTGTCTGGAACGAGTAGGTTACATGATTTGTTTGTAAAGATTGAAGGGATGACTCCGGGAGAATTTAAAAACGGAGG
TGAAAATATTAAAATTCGATACAGTTTTCAAAGAAGTGTTTTTGGTAATTATTTAATTGCTTCTACAGAAAAAGGAATTT
GTAATTTATTTTTTTATGATATTCCGGAAGAACAAATTGTTTCCGAGTTAAAAGAACAATGGAATAGGGCGGATATAATT
GAGCAAATGGATGAGAATCAAAATCGAGTCATTCGTTTTTTCGATAAAACGTTAAACGGGCATGAAAAAATCAAACTTCA
TTTGAAGGGAACCGAATTTCAAATCAAAGTTTGGGAAGCTCTTCTCAAAATTCCGGAAGGACAACTATCTTCTTATTCAG
ATATTGCAGATTTGATTGGGCAAGAAAATGCTTCTAGAGCGGTTGGAACTGCTATCGGTAAAAATCCAATAGGATATTTG
ATTCCTTGCCATAGAGTGATTAAAAGTACAGGTGGAATTGGAGAATATCGATGGGGTTCTGAAAGAAAGATGGCTATGAT
CGGTTGGGAGGCGAGTAAAGTTAAAATTTGA

Upstream 100 bases:

>100_bases
AAACTATTATTACAGAAAAATTGATTTAGAACTTACAAAATATTATAAAAATAGCAAGGATCGGGTTTCCAAAAATCCAG
ACTTCGAATAAGATTTAAAT

Downstream 100 bases:

>100_bases
CACTTAGTTTAAACCATACAAAAAATATTTTTGAGTTTGTTCTAAAATTTTAGAGTAGAGTTGTTGAAAAATTCCATAGC
GGTGATTAACAAAACTGCTT

Product: methylated-DNA--protein-cysteine methyltransferase transcription regulator

Products: NA

Alternate protein names: Regulatory protein of adaptative response; O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 276; Mature: 275

Protein sequence:

>276_residues
MSHYQKIAEAIQFIQKNAISQPELDEVAKSVNLSPFHFQRLFTEWAGVSPKQFLQYITLQNAKSILSKPQTTLFDAAFET
GLSGTSRLHDLFVKIEGMTPGEFKNGGENIKIRYSFQRSVFGNYLIASTEKGICNLFFYDIPEEQIVSELKEQWNRADII
EQMDENQNRVIRFFDKTLNGHEKIKLHLKGTEFQIKVWEALLKIPEGQLSSYSDIADLIGQENASRAVGTAIGKNPIGYL
IPCHRVIKSTGGIGEYRWGSERKMAMIGWEASKVKI

Sequences:

>Translated_276_residues
MSHYQKIAEAIQFIQKNAISQPELDEVAKSVNLSPFHFQRLFTEWAGVSPKQFLQYITLQNAKSILSKPQTTLFDAAFET
GLSGTSRLHDLFVKIEGMTPGEFKNGGENIKIRYSFQRSVFGNYLIASTEKGICNLFFYDIPEEQIVSELKEQWNRADII
EQMDENQNRVIRFFDKTLNGHEKIKLHLKGTEFQIKVWEALLKIPEGQLSSYSDIADLIGQENASRAVGTAIGKNPIGYL
IPCHRVIKSTGGIGEYRWGSERKMAMIGWEASKVKI
>Mature_275_residues
SHYQKIAEAIQFIQKNAISQPELDEVAKSVNLSPFHFQRLFTEWAGVSPKQFLQYITLQNAKSILSKPQTTLFDAAFETG
LSGTSRLHDLFVKIEGMTPGEFKNGGENIKIRYSFQRSVFGNYLIASTEKGICNLFFYDIPEEQIVSELKEQWNRADIIE
QMDENQNRVIRFFDKTLNGHEKIKLHLKGTEFQIKVWEALLKIPEGQLSSYSDIADLIGQENASRAVGTAIGKNPIGYLI
PCHRVIKSTGGIGEYRWGSERKMAMIGWEASKVKI

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus. It activates the transcription of alkA, alkB and aidB [H]

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788542, Length=272, Percent_Identity=32.3529411764706, Blast_Score=153, Evalue=1e-38,
Organism=Escherichia coli, GI1787596, Length=96, Percent_Identity=38.5416666666667, Blast_Score=76, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI115533070, Length=128, Percent_Identity=35.15625, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI115533068, Length=128, Percent_Identity=35.15625, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6320001, Length=83, Percent_Identity=40.9638554216867, Blast_Score=78, Evalue=1e-15,
Organism=Drosophila melanogaster, GI17137554, Length=171, Percent_Identity=30.4093567251462, Blast_Score=82, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR016221
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF01035 DNA_binding_1; PF00165 HTH_AraC; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 31370; Mature: 31239

Theoretical pI: Translated: 8.20; Mature: 8.20

Prosite motif: PS01124 HTH_ARAC_FAMILY_2 ; PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHYQKIAEAIQFIQKNAISQPELDEVAKSVNLSPFHFQRLFTEWAGVSPKQFLQYITLQ
CCHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHH
NAKSILSKPQTTLFDAAFETGLSGTSRLHDLFVKIEGMTPGEFKNGGENIKIRYSFQRSV
HHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHEECCCCCCCCCCCCCEEEEEEEECHHH
FGNYLIASTEKGICNLFFYDIPEEQIVSELKEQWNRADIIEQMDENQNRVIRFFDKTLNG
HCCEEEEECCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCC
HEKIKLHLKGTEFQIKVWEALLKIPEGQLSSYSDIADLIGQENASRAVGTAIGKNPIGYL
CEEEEEEEECCEEEHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCHHHHHHHHCCCCCCHH
IPCHRVIKSTGGIGEYRWGSERKMAMIGWEASKVKI
HHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCC
>Mature Secondary Structure 
SHYQKIAEAIQFIQKNAISQPELDEVAKSVNLSPFHFQRLFTEWAGVSPKQFLQYITLQ
CHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHH
NAKSILSKPQTTLFDAAFETGLSGTSRLHDLFVKIEGMTPGEFKNGGENIKIRYSFQRSV
HHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHEECCCCCCCCCCCCCEEEEEEEECHHH
FGNYLIASTEKGICNLFFYDIPEEQIVSELKEQWNRADIIEQMDENQNRVIRFFDKTLNG
HCCEEEEECCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCC
HEKIKLHLKGTEFQIKVWEALLKIPEGQLSSYSDIADLIGQENASRAVGTAIGKNPIGYL
CEEEEEEEECCEEEHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCHHHHHHHHCCCCCCHH
IPCHRVIKSTGGIGEYRWGSERKMAMIGWEASKVKI
HHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1904855; 11677609 [H]