Click here to switch to the map view.

The map label for this gene is cysI

Identifier: 45659186

GI number: 45659186

Start: 4129545

End: 4131230

Strand: Reverse

Name: cysI

Synonym: LIC13367

Alternate gene names: 45659186

Gene position: 4131230-4129545 (Counterclockwise)

Preceding gene: 45659187

Following gene: 45659183

Centisome position: 96.59

GC content: 40.69

Gene sequence:

>1686_bases
ATGTCAGAAGCAAAAGAACTATCACCCGTCGAAGATATAAAGTTAAACTCCAATAATCTAAGAGGAAAAATCGCAGAAGG
TTTAGATCAAAACATAGATACTTATGAGGAAGACGAAAAACAACTACTTAAGTTTCACGGACTTTACCAACAAAAAGACA
GAGATCGAAAAAAAGACGAAAATGGAAACGATATAGAAGCTCCTACTAGTTTTATGATTCGAGGAAGAATTCCGGGAGGA
AGGTTGACTTCCGAACAGTACCTGGTTTGGGATGAATTAGGGGATAAATTTGGAGGCGGAGCAATTCGTCTAACTACCAG
ACAATCGGTTCAGCTTCATACATTAAGAATTTTTCATCTTAGAGACGTTATGAAAGCAATTCATGAAGTGAATCTTTCCA
GTATGGGAGCTTGTGGCGACGTTGTGCGTAACGTAACTCAGGCCGTAAATCCTCTCGGTAAAAAAGAATTACAATTGTTA
GACGGAGTTTCTCAGATTTTATCCGATCATTTTAAATATAAGACCAACGCTTACGCAGAAGTATGGTTAGGTGATAAGCA
GCTCAACAAGGACGAAGAAGATCCAATTTACGGTAAAACGTATTTACCTAGAAAATTTAAGATCGCAGTAACTCTTGCTG
GAAATAATACGGTGGATATTTACGCAAACGATATGGGATTTGCGGCGACTCTTTCTTCGGACGGAAACAGAATCGACGGT
TATTTTGTATTTGCCGGAGGTGGTTTCGGAATGACTCACAACAAACCGGAGACATTTGCACGTGCGGCGAGTTTGCTCGG
TTGGGTTCCTGAAAACGCTTTGATACCGGTGGCGGAAGCGATTGTAACTGCACATAGAGATTTTGGAGATAGGACCAATC
GTAAACACGCTCGTTTAAAATATGTCCTTGCGGAAAAGGGTGTAGAATGGTTTCAATCGGAAGTCGAGTCCCGTTCTAAC
GTAAAACTAGATAAGGACAAACCATTACCAAATTGGGAAACTCCTTCTTATTTAGGTTGGAACGAAAGACAAGACGGTAC
TCTTTCTTTAGGATTTCATACCCTTGCTGGAAGAATCAAAGATTTTCCAGGTAAACCTCTGAAATCTGCTTTAAAAGAGA
TTATCGGTGCTTATAAGTTATCGGTTCAAATTACTGCCGATCAGGATTTGATTTTAATCGGAATTCAAAAGTCAGATCAA
GAGAAGGTTGAAAAAAGATTAGAAGAATTGAATGTATCTTGGAAAAGTCCTTCTCGTCTTTATGATCGTGCACTCGCTTG
TCCTGCGCTTCCTACTTGTGCACTTGCTTTAACCGAATCGGAACGTTCTTTCCCAGAAGTTTTAAACGGAATTCAAAAAG
TTTTGGATAAATTGGGGCTCAGCGATCGAGCTCCTGTGGTTCGTATGACCGGATGCCCGAACGGATGTGCAAGACCTTAC
TCTGCGGAAGTTGGAATTGTAGGCCAACAAGCCGGAGGAAAATATTCTTTATTTTTTGGGGCCGATTCGGAAGGAACCAA
GGTAGGCGAGTATGTGGCAAAAAAAATCGCACTCGCAGATATTCCCGCTCAATTGGAAAAGGCCTTTGTACTTTGGAAAA
ACGAAGGGGATTCCGACGAAAAATTCGGAGATTTTGTAAATAGATTCCCTCTTGAAAGATTTAGAGAAGCGCTTGGATCT
ATGTAA

Upstream 100 bases:

>100_bases
AGATTTGTTACAAACTCTCAAAGATGATTATTTTAGAATTCCAATCGATTCAAAAAGAATATAACGAAATTATAAACTCG
AAAAAGGAAAAATCCAAACT

Downstream 100 bases:

>100_bases
AATCTAATATTAGAAAAATTGAATTTCTTCGATTATCGGTTTCATCTTTGCGATTTTTCCGATGGTCGAAGATACCAAAT
AAATATTTCCGGCGTGGTTT

Product: sulfite reductase subunit beta

Products: NA

Alternate protein names: SiR-HP; SiRHP [H]

Number of amino acids: Translated: 561; Mature: 560

Protein sequence:

>561_residues
MSEAKELSPVEDIKLNSNNLRGKIAEGLDQNIDTYEEDEKQLLKFHGLYQQKDRDRKKDENGNDIEAPTSFMIRGRIPGG
RLTSEQYLVWDELGDKFGGGAIRLTTRQSVQLHTLRIFHLRDVMKAIHEVNLSSMGACGDVVRNVTQAVNPLGKKELQLL
DGVSQILSDHFKYKTNAYAEVWLGDKQLNKDEEDPIYGKTYLPRKFKIAVTLAGNNTVDIYANDMGFAATLSSDGNRIDG
YFVFAGGGFGMTHNKPETFARAASLLGWVPENALIPVAEAIVTAHRDFGDRTNRKHARLKYVLAEKGVEWFQSEVESRSN
VKLDKDKPLPNWETPSYLGWNERQDGTLSLGFHTLAGRIKDFPGKPLKSALKEIIGAYKLSVQITADQDLILIGIQKSDQ
EKVEKRLEELNVSWKSPSRLYDRALACPALPTCALALTESERSFPEVLNGIQKVLDKLGLSDRAPVVRMTGCPNGCARPY
SAEVGIVGQQAGGKYSLFFGADSEGTKVGEYVAKKIALADIPAQLEKAFVLWKNEGDSDEKFGDFVNRFPLERFREALGS
M

Sequences:

>Translated_561_residues
MSEAKELSPVEDIKLNSNNLRGKIAEGLDQNIDTYEEDEKQLLKFHGLYQQKDRDRKKDENGNDIEAPTSFMIRGRIPGG
RLTSEQYLVWDELGDKFGGGAIRLTTRQSVQLHTLRIFHLRDVMKAIHEVNLSSMGACGDVVRNVTQAVNPLGKKELQLL
DGVSQILSDHFKYKTNAYAEVWLGDKQLNKDEEDPIYGKTYLPRKFKIAVTLAGNNTVDIYANDMGFAATLSSDGNRIDG
YFVFAGGGFGMTHNKPETFARAASLLGWVPENALIPVAEAIVTAHRDFGDRTNRKHARLKYVLAEKGVEWFQSEVESRSN
VKLDKDKPLPNWETPSYLGWNERQDGTLSLGFHTLAGRIKDFPGKPLKSALKEIIGAYKLSVQITADQDLILIGIQKSDQ
EKVEKRLEELNVSWKSPSRLYDRALACPALPTCALALTESERSFPEVLNGIQKVLDKLGLSDRAPVVRMTGCPNGCARPY
SAEVGIVGQQAGGKYSLFFGADSEGTKVGEYVAKKIALADIPAQLEKAFVLWKNEGDSDEKFGDFVNRFPLERFREALGS
M
>Mature_560_residues
SEAKELSPVEDIKLNSNNLRGKIAEGLDQNIDTYEEDEKQLLKFHGLYQQKDRDRKKDENGNDIEAPTSFMIRGRIPGGR
LTSEQYLVWDELGDKFGGGAIRLTTRQSVQLHTLRIFHLRDVMKAIHEVNLSSMGACGDVVRNVTQAVNPLGKKELQLLD
GVSQILSDHFKYKTNAYAEVWLGDKQLNKDEEDPIYGKTYLPRKFKIAVTLAGNNTVDIYANDMGFAATLSSDGNRIDGY
FVFAGGGFGMTHNKPETFARAASLLGWVPENALIPVAEAIVTAHRDFGDRTNRKHARLKYVLAEKGVEWFQSEVESRSNV
KLDKDKPLPNWETPSYLGWNERQDGTLSLGFHTLAGRIKDFPGKPLKSALKEIIGAYKLSVQITADQDLILIGIQKSDQE
KVEKRLEELNVSWKSPSRLYDRALACPALPTCALALTESERSFPEVLNGIQKVLDKLGLSDRAPVVRMTGCPNGCARPYS
AEVGIVGQQAGGKYSLFFGADSEGTKVGEYVAKKIALADIPAQLEKAFVLWKNEGDSDEKFGDFVNRFPLERFREALGSM

Specific function: Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L- cysteine from sulfate [H]

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Escherichia coli, GI1789122, Length=550, Percent_Identity=37.2727272727273, Blast_Score=373, Evalue=1e-104,
Organism=Saccharomyces cerevisiae, GI6322597, Length=579, Percent_Identity=34.0241796200345, Blast_Score=340, Evalue=3e-94,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011786
- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066 [H]

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: =1.8.1.2 [H]

Molecular weight: Translated: 62469; Mature: 62337

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEAKELSPVEDIKLNSNNLRGKIAEGLDQNIDTYEEDEKQLLKFHGLYQQKDRDRKKDE
CCCCHHCCCHHHEEECCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
NGNDIEAPTSFMIRGRIPGGRLTSEQYLVWDELGDKFGGGAIRLTTRQSVQLHTLRIFHL
CCCCCCCCCEEEEEECCCCCCCCCCCEEEHHHHCCCCCCCEEEEEECCCEEEEHHHHHHH
RDVMKAIHEVNLSSMGACGDVVRNVTQAVNPLGKKELQLLDGVSQILSDHFKYKTNAYAE
HHHHHHHHHCCCHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEE
VWLGDKQLNKDEEDPIYGKTYLPRKFKIAVTLAGNNTVDIYANDMGFAATLSSDGNRIDG
EEECCHHCCCCCCCCCCCCCCCCCEEEEEEEEECCCEEEEEECCCCEEEEECCCCCEECE
YFVFAGGGFGMTHNKPETFARAASLLGWVPENALIPVAEAIVTAHRDFGDRTNRKHARLK
EEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHH
YVLAEKGVEWFQSEVESRSNVKLDKDKPLPNWETPSYLGWNERQDGTLSLGFHTLAGRIK
HHHHHHCHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHH
DFPGKPLKSALKEIIGAYKLSVQITADQDLILIGIQKSDQEKVEKRLEELNVSWKSPSRL
CCCCCHHHHHHHHHHCCEEEEEEEECCCCEEEEEECCCCHHHHHHHHHHCCCCCCCHHHH
YDRALACPALPTCALALTESERSFPEVLNGIQKVLDKLGLSDRAPVVRMTGCPNGCARPY
HHHHHCCCCCCHHHEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCC
SAEVGIVGQQAGGKYSLFFGADSEGTKVGEYVAKKIALADIPAQLEKAFVLWKNEGDSDE
CCCEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCHHHHCCEEEEECCCCCCHH
KFGDFVNRFPLERFREALGSM
HHHHHHHHCCHHHHHHHHCCC
>Mature Secondary Structure 
SEAKELSPVEDIKLNSNNLRGKIAEGLDQNIDTYEEDEKQLLKFHGLYQQKDRDRKKDE
CCCHHCCCHHHEEECCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
NGNDIEAPTSFMIRGRIPGGRLTSEQYLVWDELGDKFGGGAIRLTTRQSVQLHTLRIFHL
CCCCCCCCCEEEEEECCCCCCCCCCCEEEHHHHCCCCCCCEEEEEECCCEEEEHHHHHHH
RDVMKAIHEVNLSSMGACGDVVRNVTQAVNPLGKKELQLLDGVSQILSDHFKYKTNAYAE
HHHHHHHHHCCCHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEE
VWLGDKQLNKDEEDPIYGKTYLPRKFKIAVTLAGNNTVDIYANDMGFAATLSSDGNRIDG
EEECCHHCCCCCCCCCCCCCCCCCEEEEEEEEECCCEEEEEECCCCEEEEECCCCCEECE
YFVFAGGGFGMTHNKPETFARAASLLGWVPENALIPVAEAIVTAHRDFGDRTNRKHARLK
EEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHH
YVLAEKGVEWFQSEVESRSNVKLDKDKPLPNWETPSYLGWNERQDGTLSLGFHTLAGRIK
HHHHHHCHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHH
DFPGKPLKSALKEIIGAYKLSVQITADQDLILIGIQKSDQEKVEKRLEELNVSWKSPSRL
CCCCCHHHHHHHHHHCCEEEEEEEECCCCEEEEEECCCCHHHHHHHHHHCCCCCCCHHHH
YDRALACPALPTCALALTESERSFPEVLNGIQKVLDKLGLSDRAPVVRMTGCPNGCARPY
HHHHHCCCCCCHHHEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCC
SAEVGIVGQQAGGKYSLFFGADSEGTKVGEYVAKKIALADIPAQLEKAFVLWKNEGDSDE
CCCEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCHHHHCCEEEEECCCCCCHH
KFGDFVNRFPLERFREALGSM
HHHHHHHHCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA