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Identifier: 45659103

GI number: 45659103

Start: 4024999

End: 4027044

Strand: Reverse

Name: lig

Synonym: LIC13282

Alternate gene names: 45659103

Gene position: 4027044-4024999 (Counterclockwise)

Preceding gene: 45659104

Following gene: 45659102

Centisome position: 94.15

GC content: 37.15

Gene sequence:

>2046_bases
ATGCCAAAGAAGAAGGAAGACTCCCAGAAAACTCTTTCGGAGAAAGAGGCCAAGGGACTTATAGCCAAACTCTCCGACGA
AATACGTCATCACCAATATCTCTATTATGTAAAAAACGATCCGAAAATTTCTGATTTTGATTTCGATCAGTTATTCAGGC
GACTTCAAGATTTAGAAGAAGAGTTCCCGCAGTTTAAGGATCTTGCAAGTCCTACTTTGGTTGTAGGTTCTGATTTAGAT
AAGGACTTTGAAAAGTTTCAACATAAACTTCCTGTATTATCTCTTATCAATACGTATAATGATAATGAGTTATTAGAATG
GGTCAATAAGACTGATCCGGAAGGATTGTATTCCGTAGAATGGAAAATAGATGGGGCTTCTATTGTACTATATTATGAAA
ATGGAATTCTTAAAAACGGAGTTACTAGAGGTTCCGGCGGAATTGGAGATGATGTTACAGATAATATCCGGACTATTCGG
AATATTCCTCTTCGTTTGCCGGAGCCGATAACGGTTTATCTTCGCGGCGAAGTTTTTATGACTTTTAAAGATTTTGAAGA
ATTTAACGCACTTTCTTCCGGTAAATATGCAAATCCGCGAAATTTGTCTGCCGGATCTATCAAACAAAAAAATTCCTCGG
ATACAGCAAAACGTCCTCTTAGGATTTTTACTTACGACGCAACTTTCCCGAATATGGAAAAAAAATTTAAAACACACCAA
GAAATTTTTTCCAAACTCGAAAAGCTTACGTTTCCAGTTCCGCCTAACACGGCCTTTGTGAGCGGATCTAAAATTGCAAA
AACTATCCAGGAATTTAAAAAGCAAAAAGATTCTTTGGGATTTCCGACCGACGGTCTTGTTATCAAACTAAACGATATTT
CTAAGCGGGATGCACTTGGTTATACTTCTCATTCTCCTCGATGGGCAAGAGCGTATAAATTTGATGCAATCATGAAAGAA
AGTAAGATTGTAGATATAACTTATGCAGTTGGTCGAACTGGAAAGATTACTCCTAGAGCGGAAATAGAACCAATAAGTCT
TGCGGGTACTACCGTTACATTTGCGACTCTTCATAATCAAGATTATATAGACGAACTTGGAGTAGGGATCGGTGCGATCG
TAAGAGTGGCAAAACGTGGAGAAATTATTCCTGCGGTAGAAGAAGTAGTTACTCCTGGTAAAGAAGTTTTTAAAATTCCG
GATCGTTGTCCTTCTTGTAATACACAAACGATTAAGAAAGAAAGTTTAGTCGATCTATTTTGTCCTAATCCGGATTGTCC
CGATCGTGTGAAAAATGGAATTATCTTTTACTGTCAGAGAAAGCAGATGGATATAGAAGGACTCGGGGATAAACAGATCG
AGTTCTTGTATGATCACGATTATATAAAGTCTATTGCGGACTTGTATGATCTGAAGGATCAAAAAGAGAAGTTAATGGAA
GAAGAAGGTTTTGGAGAAAAGAGTGTAAACATTATTCTCAAAGGGATAGAACAATCCAAACAAAAGGATTTTCGTTTTTT
ACTTCCTTCTCTTGGACTCTCGGAGCTTGGTCATAAGGTAACGGAATTGTTAATCGAACACGGGATCGATTCTATAGACG
AAATTCTTTCTATTGCGAAAGATCAGAAAAAAATCGAATCTCTTTTAGAAATTCCTGGAATTGGGCCTTCTACAATTCAA
GCTTTTCAGGAAAATTTTTCTGATAAAAGAATTTTAAAACTAATAGAACGTCTTAAAAAGGCGGGTCTTAAAATGAAAGC
GGACCCCATTCAGGTTGCGGACCAACAACCGTTTGCGGGGCAATCTTGGTGTGTAACCGGTTCTTTTGAAAACTTCCAGC
CAAGAGATAAAGCGATGGATCTGATTGTTTATTATGGTGGAAGAAAGGTGAGTGCTGTAAGTTCAAAAACTACTCATCTT
TTAGCTGGACCTGGTGCCGGATCTAAATTAGAAAAGGCGAATGAACTTGGAGTTTCTGTTTATGATGAAAAACAATTTTT
GGATTTGTTGAAGTCTTTAAAAATTGATTTCAAAAATCTTATTTAG

Upstream 100 bases:

>100_bases
ACCTCATTTACATTATGAAATTTGGATTGGAGAATCCAATCGCACCGATCCTATGGAATATCTCAAAGTTCCAGTTTATT
AATCTCAATCCGGATACAAT

Downstream 100 bases:

>100_bases
AGTTTGTTGTAAAGCGGTTAAATATAATTTTATATATTATAAAATTATATTTTTTTAAAATGTACTTTGGGAATTTTAAG
TTTTCTGTAAAATGTAATGA

Product: NAD dependent DNA ligase

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 681; Mature: 680

Protein sequence:

>681_residues
MPKKKEDSQKTLSEKEAKGLIAKLSDEIRHHQYLYYVKNDPKISDFDFDQLFRRLQDLEEEFPQFKDLASPTLVVGSDLD
KDFEKFQHKLPVLSLINTYNDNELLEWVNKTDPEGLYSVEWKIDGASIVLYYENGILKNGVTRGSGGIGDDVTDNIRTIR
NIPLRLPEPITVYLRGEVFMTFKDFEEFNALSSGKYANPRNLSAGSIKQKNSSDTAKRPLRIFTYDATFPNMEKKFKTHQ
EIFSKLEKLTFPVPPNTAFVSGSKIAKTIQEFKKQKDSLGFPTDGLVIKLNDISKRDALGYTSHSPRWARAYKFDAIMKE
SKIVDITYAVGRTGKITPRAEIEPISLAGTTVTFATLHNQDYIDELGVGIGAIVRVAKRGEIIPAVEEVVTPGKEVFKIP
DRCPSCNTQTIKKESLVDLFCPNPDCPDRVKNGIIFYCQRKQMDIEGLGDKQIEFLYDHDYIKSIADLYDLKDQKEKLME
EEGFGEKSVNIILKGIEQSKQKDFRFLLPSLGLSELGHKVTELLIEHGIDSIDEILSIAKDQKKIESLLEIPGIGPSTIQ
AFQENFSDKRILKLIERLKKAGLKMKADPIQVADQQPFAGQSWCVTGSFENFQPRDKAMDLIVYYGGRKVSAVSSKTTHL
LAGPGAGSKLEKANELGVSVYDEKQFLDLLKSLKIDFKNLI

Sequences:

>Translated_681_residues
MPKKKEDSQKTLSEKEAKGLIAKLSDEIRHHQYLYYVKNDPKISDFDFDQLFRRLQDLEEEFPQFKDLASPTLVVGSDLD
KDFEKFQHKLPVLSLINTYNDNELLEWVNKTDPEGLYSVEWKIDGASIVLYYENGILKNGVTRGSGGIGDDVTDNIRTIR
NIPLRLPEPITVYLRGEVFMTFKDFEEFNALSSGKYANPRNLSAGSIKQKNSSDTAKRPLRIFTYDATFPNMEKKFKTHQ
EIFSKLEKLTFPVPPNTAFVSGSKIAKTIQEFKKQKDSLGFPTDGLVIKLNDISKRDALGYTSHSPRWARAYKFDAIMKE
SKIVDITYAVGRTGKITPRAEIEPISLAGTTVTFATLHNQDYIDELGVGIGAIVRVAKRGEIIPAVEEVVTPGKEVFKIP
DRCPSCNTQTIKKESLVDLFCPNPDCPDRVKNGIIFYCQRKQMDIEGLGDKQIEFLYDHDYIKSIADLYDLKDQKEKLME
EEGFGEKSVNIILKGIEQSKQKDFRFLLPSLGLSELGHKVTELLIEHGIDSIDEILSIAKDQKKIESLLEIPGIGPSTIQ
AFQENFSDKRILKLIERLKKAGLKMKADPIQVADQQPFAGQSWCVTGSFENFQPRDKAMDLIVYYGGRKVSAVSSKTTHL
LAGPGAGSKLEKANELGVSVYDEKQFLDLLKSLKIDFKNLI
>Mature_680_residues
PKKKEDSQKTLSEKEAKGLIAKLSDEIRHHQYLYYVKNDPKISDFDFDQLFRRLQDLEEEFPQFKDLASPTLVVGSDLDK
DFEKFQHKLPVLSLINTYNDNELLEWVNKTDPEGLYSVEWKIDGASIVLYYENGILKNGVTRGSGGIGDDVTDNIRTIRN
IPLRLPEPITVYLRGEVFMTFKDFEEFNALSSGKYANPRNLSAGSIKQKNSSDTAKRPLRIFTYDATFPNMEKKFKTHQE
IFSKLEKLTFPVPPNTAFVSGSKIAKTIQEFKKQKDSLGFPTDGLVIKLNDISKRDALGYTSHSPRWARAYKFDAIMKES
KIVDITYAVGRTGKITPRAEIEPISLAGTTVTFATLHNQDYIDELGVGIGAIVRVAKRGEIIPAVEEVVTPGKEVFKIPD
RCPSCNTQTIKKESLVDLFCPNPDCPDRVKNGIIFYCQRKQMDIEGLGDKQIEFLYDHDYIKSIADLYDLKDQKEKLMEE
EGFGEKSVNIILKGIEQSKQKDFRFLLPSLGLSELGHKVTELLIEHGIDSIDEILSIAKDQKKIESLLEIPGIGPSTIQA
FQENFSDKRILKLIERLKKAGLKMKADPIQVADQQPFAGQSWCVTGSFENFQPRDKAMDLIVYYGGRKVSAVSSKTTHLL
AGPGAGSKLEKANELGVSVYDEKQFLDLLKSLKIDFKNLI

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=675, Percent_Identity=34.0740740740741, Blast_Score=374, Evalue=1e-104,
Organism=Escherichia coli, GI87082305, Length=510, Percent_Identity=21.1764705882353, Blast_Score=91, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_LEPIC (Q72MA6)

Other databases:

- EMBL:   AE016823
- RefSeq:   YP_003189.1
- HSSP:   Q9ZHI0
- ProteinModelPortal:   Q72MA6
- SMR:   Q72MA6
- GeneID:   2771889
- GenomeReviews:   AE016823_GR
- KEGG:   lic:LIC13282
- NMPDR:   fig|267671.1.peg.3189
- HOGENOM:   HBG620317
- OMA:   HNQDYID
- ProtClustDB:   CLSK575130
- BioCyc:   LINT267671:LIC_13282-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 76914; Mature: 76783

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2; PS00107 PROTEIN_KINASE_ATP

Important sites: ACT_SITE 122-122 BINDING 120-120 BINDING 143-143 BINDING 177-177 BINDING 289-289 BINDING 313-313

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKKKEDSQKTLSEKEAKGLIAKLSDEIRHHQYLYYVKNDPKISDFDFDQLFRRLQDLEE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHH
EFPQFKDLASPTLVVGSDLDKDFEKFQHKLPVLSLINTYNDNELLEWVNKTDPEGLYSVE
HHHHHHHHCCCEEEECCCCCHHHHHHHHHCHHHHHHHCCCCHHHHHHHCCCCCCCEEEEE
WKIDGASIVLYYENGILKNGVTRGSGGIGDDVTDNIRTIRNIPLRLPEPITVYLRGEVFM
EEECCEEEEEEEECCEEECCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEEECEEEE
TFKDFEEFNALSSGKYANPRNLSAGSIKQKNSSDTAKRPLRIFTYDATFPNMEKKFKTHQ
EEHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHCCEEEEEECCCCCCHHHHHHHHH
EIFSKLEKLTFPVPPNTAFVSGSKIAKTIQEFKKQKDSLGFPTDGLVIKLNDISKRDALG
HHHHHHHHCCCCCCCCCEEECCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHCCC
YTSHSPRWARAYKFDAIMKESKIVDITYAVGRTGKITPRAEIEPISLAGTTVTFATLHNQ
CCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCEEEECCCEEEEEEECCC
DYIDELGVGIGAIVRVAKRGEIIPAVEEVVTPGKEVFKIPDRCPSCNTQTIKKESLVDLF
HHHHHHCCCHHHHHHHHHCCCCCHHHHHHHCCCHHHEECCCCCCCCCCCHHHHHCCCEEE
CPNPDCPDRVKNGIIFYCQRKQMDIEGLGDKQIEFLYDHDYIKSIADLYDLKDQKEKLME
CCCCCCHHHHCCCEEEEEECCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHH
EEGFGEKSVNIILKGIEQSKQKDFRFLLPSLGLSELGHKVTELLIEHGIDSIDEILSIAK
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHH
DQKKIESLLEIPGIGPSTIQAFQENFSDKRILKLIERLKKAGLKMKADPIQVADQQPFAG
HHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCEECCCCCEECCCCCCCC
QSWCVTGSFENFQPRDKAMDLIVYYGGRKVSAVSSKTTHLLAGPGAGSKLEKANELGVSV
CCEEEECCCCCCCCHHHHEEEEEEECCEEEEEECCCCEEEEECCCCCCHHHHHHHCCCEE
YDEKQFLDLLKSLKIDFKNLI
ECHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PKKKEDSQKTLSEKEAKGLIAKLSDEIRHHQYLYYVKNDPKISDFDFDQLFRRLQDLEE
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHH
EFPQFKDLASPTLVVGSDLDKDFEKFQHKLPVLSLINTYNDNELLEWVNKTDPEGLYSVE
HHHHHHHHCCCEEEECCCCCHHHHHHHHHCHHHHHHHCCCCHHHHHHHCCCCCCCEEEEE
WKIDGASIVLYYENGILKNGVTRGSGGIGDDVTDNIRTIRNIPLRLPEPITVYLRGEVFM
EEECCEEEEEEEECCEEECCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEEECEEEE
TFKDFEEFNALSSGKYANPRNLSAGSIKQKNSSDTAKRPLRIFTYDATFPNMEKKFKTHQ
EEHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHCCEEEEEECCCCCCHHHHHHHHH
EIFSKLEKLTFPVPPNTAFVSGSKIAKTIQEFKKQKDSLGFPTDGLVIKLNDISKRDALG
HHHHHHHHCCCCCCCCCEEECCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHCCC
YTSHSPRWARAYKFDAIMKESKIVDITYAVGRTGKITPRAEIEPISLAGTTVTFATLHNQ
CCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCEEEECCCEEEEEEECCC
DYIDELGVGIGAIVRVAKRGEIIPAVEEVVTPGKEVFKIPDRCPSCNTQTIKKESLVDLF
HHHHHHCCCHHHHHHHHHCCCCCHHHHHHHCCCHHHEECCCCCCCCCCCHHHHHCCCEEE
CPNPDCPDRVKNGIIFYCQRKQMDIEGLGDKQIEFLYDHDYIKSIADLYDLKDQKEKLME
CCCCCCHHHHCCCEEEEEECCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHH
EEGFGEKSVNIILKGIEQSKQKDFRFLLPSLGLSELGHKVTELLIEHGIDSIDEILSIAK
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHH
DQKKIESLLEIPGIGPSTIQAFQENFSDKRILKLIERLKKAGLKMKADPIQVADQQPFAG
HHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCEECCCCCEECCCCCCCC
QSWCVTGSFENFQPRDKAMDLIVYYGGRKVSAVSSKTTHLLAGPGAGSKLEKANELGVSV
CCEEEECCCCCCCCHHHHEEEEEEECCEEEEEECCCCEEEEECCCCCCHHHHHHHCCCEE
YDEKQFLDLLKSLKIDFKNLI
ECHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA