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The map label for this gene is purF

Identifier: 45659083

GI number: 45659083

Start: 3999615

End: 4001087

Strand: Reverse

Name: purF

Synonym: LIC13262

Alternate gene names: 45659083

Gene position: 4001087-3999615 (Counterclockwise)

Preceding gene: 45659095

Following gene: 45659082

Centisome position: 93.54

GC content: 40.53

Gene sequence:

>1473_bases
ATGAGTCGAATTCCCGATAAATCCAGAATAAGAAGACAAGCCCAGGATGACAAACCAAAAGAGGAATGTGCGATTTTTGG
TATATTTAATTCATCGGAGGCTTCTAATTTCACCTATTTAGGTCTTTACTCGATGCAACATCGGGGGCAGGAATCGAGCG
GAATCGTTTCTTCGGACGGGGAACACTTATATCGTTATGCTGGAATGGGCTTGGTGGCTCATATCTTTACGGAAACCAAA
TTAAAGGAACTGCAAGGAAATGCGGCTATCGGCCACAATCGATATTCTACAACTGGGGCTTCTTTTTTAAGAAATGCTCA
GCCTCTTCGAGTAGAATCCCACTTAGGAGCCGTTTCTTTGGCTCATAATGGAAATTTGGTTAATTCTTGGGAACTTAGAA
GCCAACTCGAAAAAGAAGGAAGTATCTTTCAGACTACGATTGATTCCGAAGTAATTGTTCATCTAATGGCTCGTTCTGGA
GAAACGGACTTTCTTTCTGCTCTTTCTTCCGCTCTTAAAAAAGTAAGAGGGGCATATTCACTTGTAATTCTTACAAAAAC
CCAATTGATTGCAGTCAGAGATCCAAATGGGTTTCGTCCTTTAGTGATGGGTCGTCGAGAAGACGGATCGATCGTATTTG
CTTCGGAAACCTGTGCGTTTGATATTACGGATACGAAATATGAAAGAGATGTAGAACCGGGCGAGATGATCGTAGTTGAT
AAGAATGGAATGAATTCTTATTATCCGTTTCCAAAAGCTTCCCCGAGTCTTTGTATTTTTGAGTATATCTATTTCGCAAG
ACCCGATTCTAGTATCTTTGGAGAGTCGGTTTATAAAGTTCGAAAGAATTTAGGAAGATTTTTAGCTAGAGAATTACCCG
TTCCAGCGGATGTAGTCATTCCAGTTCCGGACTCTGCCAACATAGCTGCGTTAGGTTATGCAGAAGAATCTGGAATTTCT
TATCAATCTGGTTTGATCCGTTCTCATTACATCGGTAGAACTTTTATAGAGCCGGATCAGAAAATTCGGGATTTTGGAGC
TAAGATTAAATACAATGTAGTTCGAAACGTAGTCGAAGGAAAAAGGGTGATCGTAGTTGACGATTCGATTATGAGAGGAA
CGACGAGTCGAAAAATCATCAAAATGATTCGAAATGCTGGCGCTAAAGAAATTCATCTTAGAGTTTCTGCACCTCCTACT
ATATCACCTTGTTATTATGGAATCGATATTCCTACTCATAACGAATTGATCGCGGCGACTCACACGATCGAAGAAATTCG
AAAGTATTTAAGAGTGGATAGTATCGCTTATCTTTCCGTCGAGTCTATGAACCGTGCCGTAATGGATCATAAAGGTGGTG
GGTTTTGTAACGCGTGTTTTACGGCTCAATACCCAGTGGAGTTTCAGAGCGAGTTAGGAAACCAGAAAAGTCTTTTTAAA
GAATATCAAGTGGAAGAAAGGGTCGTTTATTGA

Upstream 100 bases:

>100_bases
AGGACTAGTTGTAGGCTTCGAGCTGTGTCAACGACTATGTAATCGGAATTTATTTGCATGAAAAACGCAACCTGAGATTT
TAAGCTTAAGAAGGCAGAGA

Downstream 100 bases:

>100_bases
GTTTGTTCCAAAGTTATTGAATAGAATTTGGCTTTGGACGAATCCTGCTAACTGATTATAAAAACAAGTTTAAATGAAAT
GTGGGACCTGGCTCTCAGCT

Product: glutamine phosphoribosylpyrophosphate amidotransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 490; Mature: 489

Protein sequence:

>490_residues
MSRIPDKSRIRRQAQDDKPKEECAIFGIFNSSEASNFTYLGLYSMQHRGQESSGIVSSDGEHLYRYAGMGLVAHIFTETK
LKELQGNAAIGHNRYSTTGASFLRNAQPLRVESHLGAVSLAHNGNLVNSWELRSQLEKEGSIFQTTIDSEVIVHLMARSG
ETDFLSALSSALKKVRGAYSLVILTKTQLIAVRDPNGFRPLVMGRREDGSIVFASETCAFDITDTKYERDVEPGEMIVVD
KNGMNSYYPFPKASPSLCIFEYIYFARPDSSIFGESVYKVRKNLGRFLARELPVPADVVIPVPDSANIAALGYAEESGIS
YQSGLIRSHYIGRTFIEPDQKIRDFGAKIKYNVVRNVVEGKRVIVVDDSIMRGTTSRKIIKMIRNAGAKEIHLRVSAPPT
ISPCYYGIDIPTHNELIAATHTIEEIRKYLRVDSIAYLSVESMNRAVMDHKGGGFCNACFTAQYPVEFQSELGNQKSLFK
EYQVEERVVY

Sequences:

>Translated_490_residues
MSRIPDKSRIRRQAQDDKPKEECAIFGIFNSSEASNFTYLGLYSMQHRGQESSGIVSSDGEHLYRYAGMGLVAHIFTETK
LKELQGNAAIGHNRYSTTGASFLRNAQPLRVESHLGAVSLAHNGNLVNSWELRSQLEKEGSIFQTTIDSEVIVHLMARSG
ETDFLSALSSALKKVRGAYSLVILTKTQLIAVRDPNGFRPLVMGRREDGSIVFASETCAFDITDTKYERDVEPGEMIVVD
KNGMNSYYPFPKASPSLCIFEYIYFARPDSSIFGESVYKVRKNLGRFLARELPVPADVVIPVPDSANIAALGYAEESGIS
YQSGLIRSHYIGRTFIEPDQKIRDFGAKIKYNVVRNVVEGKRVIVVDDSIMRGTTSRKIIKMIRNAGAKEIHLRVSAPPT
ISPCYYGIDIPTHNELIAATHTIEEIRKYLRVDSIAYLSVESMNRAVMDHKGGGFCNACFTAQYPVEFQSELGNQKSLFK
EYQVEERVVY
>Mature_489_residues
SRIPDKSRIRRQAQDDKPKEECAIFGIFNSSEASNFTYLGLYSMQHRGQESSGIVSSDGEHLYRYAGMGLVAHIFTETKL
KELQGNAAIGHNRYSTTGASFLRNAQPLRVESHLGAVSLAHNGNLVNSWELRSQLEKEGSIFQTTIDSEVIVHLMARSGE
TDFLSALSSALKKVRGAYSLVILTKTQLIAVRDPNGFRPLVMGRREDGSIVFASETCAFDITDTKYERDVEPGEMIVVDK
NGMNSYYPFPKASPSLCIFEYIYFARPDSSIFGESVYKVRKNLGRFLARELPVPADVVIPVPDSANIAALGYAEESGISY
QSGLIRSHYIGRTFIEPDQKIRDFGAKIKYNVVRNVVEGKRVIVVDDSIMRGTTSRKIIKMIRNAGAKEIHLRVSAPPTI
SPCYYGIDIPTHNELIAATHTIEEIRKYLRVDSIAYLSVESMNRAVMDHKGGGFCNACFTAQYPVEFQSELGNQKSLFKE
YQVEERVVY

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=508, Percent_Identity=39.7637795275591, Blast_Score=360, Evalue=1e-99,
Organism=Escherichia coli, GI1788651, Length=449, Percent_Identity=38.7527839643653, Blast_Score=290, Evalue=1e-79,
Organism=Escherichia coli, GI1790167, Length=189, Percent_Identity=31.7460317460317, Blast_Score=79, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17554892, Length=478, Percent_Identity=40.3765690376569, Blast_Score=350, Evalue=1e-96,
Organism=Saccharomyces cerevisiae, GI6323958, Length=470, Percent_Identity=35.1063829787234, Blast_Score=263, Evalue=4e-71,
Organism=Drosophila melanogaster, GI28573187, Length=493, Percent_Identity=39.553752535497, Blast_Score=352, Evalue=5e-97,
Organism=Drosophila melanogaster, GI24659598, Length=490, Percent_Identity=38.7755102040816, Blast_Score=347, Evalue=1e-95,
Organism=Drosophila melanogaster, GI24659604, Length=466, Percent_Identity=40.1287553648069, Blast_Score=343, Evalue=1e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 54782; Mature: 54651

Theoretical pI: Translated: 8.12; Mature: 8.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRIPDKSRIRRQAQDDKPKEECAIFGIFNSSEASNFTYLGLYSMQHRGQESSGIVSSDG
CCCCCCHHHHHHHHCCCCCHHHEEEEEEECCCCCCCEEEEEEEHHHHCCCCCCCCCCCCC
EHLYRYAGMGLVAHIFTETKLKELQGNAAIGHNRYSTTGASFLRNAQPLRVESHLGAVSL
HHHHHHHCCHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHCCCCCEEECCCCCEEEE
AHNGNLVNSWELRSQLEKEGSIFQTTIDSEVIVHLMARSGETDFLSALSSALKKVRGAYS
ECCCCCCCHHHHHHHHHHCCCEEEEECCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEE
LVILTKTQLIAVRDPNGFRPLVMGRREDGSIVFASETCAFDITDTKYERDVEPGEMIVVD
EEEEEEEEEEEEECCCCCCCEEEECCCCCCEEEEECCEEEECCCCCHHCCCCCCCEEEEE
KNGMNSYYPFPKASPSLCIFEYIYFARPDSSIFGESVYKVRKNLGRFLARELPVPADVVI
CCCCCCCCCCCCCCCCCEEEEEHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
PVPDSANIAALGYAEESGISYQSGLIRSHYIGRTFIEPDQKIRDFGAKIKYNVVRNVVEG
ECCCCCCEEEEECCHHCCCCHHHCCHHHHCCCCCCCCCCHHHHHHCCCEEHHHHHHHHCC
KRVIVVDDSIMRGTTSRKIIKMIRNAGAKEIHLRVSAPPTISPCYYGIDIPTHNELIAAT
CEEEEECCHHHCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEEEECCCCCCEEEHH
HTIEEIRKYLRVDSIAYLSVESMNRAVMDHKGGGFCNACFTAQYPVEFQSELGNQKSLFK
HHHHHHHHHHHHCCEEEEEHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHCCCHHHHHH
EYQVEERVVY
HHHHHHHCCC
>Mature Secondary Structure 
SRIPDKSRIRRQAQDDKPKEECAIFGIFNSSEASNFTYLGLYSMQHRGQESSGIVSSDG
CCCCCHHHHHHHHCCCCCHHHEEEEEEECCCCCCCEEEEEEEHHHHCCCCCCCCCCCCC
EHLYRYAGMGLVAHIFTETKLKELQGNAAIGHNRYSTTGASFLRNAQPLRVESHLGAVSL
HHHHHHHCCHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHCCCCCEEECCCCCEEEE
AHNGNLVNSWELRSQLEKEGSIFQTTIDSEVIVHLMARSGETDFLSALSSALKKVRGAYS
ECCCCCCCHHHHHHHHHHCCCEEEEECCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEE
LVILTKTQLIAVRDPNGFRPLVMGRREDGSIVFASETCAFDITDTKYERDVEPGEMIVVD
EEEEEEEEEEEEECCCCCCCEEEECCCCCCEEEEECCEEEECCCCCHHCCCCCCCEEEEE
KNGMNSYYPFPKASPSLCIFEYIYFARPDSSIFGESVYKVRKNLGRFLARELPVPADVVI
CCCCCCCCCCCCCCCCCEEEEEHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
PVPDSANIAALGYAEESGISYQSGLIRSHYIGRTFIEPDQKIRDFGAKIKYNVVRNVVEG
ECCCCCCEEEEECCHHCCCCHHHCCHHHHCCCCCCCCCCHHHHHHCCCEEHHHHHHHHCC
KRVIVVDDSIMRGTTSRKIIKMIRNAGAKEIHLRVSAPPTISPCYYGIDIPTHNELIAAT
CEEEEECCHHHCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEEEECCCCCCEEEHH
HTIEEIRKYLRVDSIAYLSVESMNRAVMDHKGGGFCNACFTAQYPVEFQSELGNQKSLFK
HHHHHHHHHHHHCCEEEEEHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHCCCHHHHHH
EYQVEERVVY
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6411717; 3036807; 9384377; 6411716; 6094545; 3090047; 8197456; 9271502 [H]