Click here to switch to the map view.

The map label for this gene is icdA

Identifier: 45659065

GI number: 45659065

Start: 3979829

End: 3981025

Strand: Reverse

Name: icdA

Synonym: LIC13244

Alternate gene names: 45659065

Gene position: 3981025-3979829 (Counterclockwise)

Preceding gene: 45659066

Following gene: 45659064

Centisome position: 93.08

GC content: 42.27

Gene sequence:

>1197_bases
ATGGCAAAGATCAAGGTAAAAACCCCGCTCGTGGAGCTTGACGGGGACGAGATGACCAGGATCATCTGGAAGGAGATTAA
GGATAGATTCATTCATCCGTATCTCGATATAGAACTGGATTATTATGATTTAGGCGTGGAGTACAGGGACAAAACGGATG
ATAAAGTAACTGTGGATTCTGCTCACGCGATTCAAAAATATGGAGTAGGCGTAAAATGTGCGACGATTACTCCTAACCAA
GATAGAGTTAAAGAATACAATCTCAAACAAGAATGGAAATCTCCGAACGGAACCATTCGTTCTATTTTAGACGGAACCGT
TTTTCGTAAACCGATCATCGTAAAAAATATTCCTCCTGCGGTTCGTTCTTGGAAAAAACCGATTACAGTAGGACGTCACG
CTTACGGAGATCTTTATAAGGACACAGAACTTTATATTCCCGAAGCCGGAAAGGTGGAACTCGTTTATACCGGAAAGGAC
GGAAAAGAAAAACAAAGAGCTTTGATTCACGACTTTGAAGGAGCCGGTGTGATCATGGGGCAACATAACTTGGATAAGTC
GATCTTAAGTTTTGCCCAGGCTTGTTTTAACTACGCTATTTCCGAAAAGATTGATCTTTGGTTCGCTACAAAGGACACAA
TCTCCAAAAAATATCACGCCAGATTCCGCGCCATCTTTGACGAACTTGCAAAGGCTAAAGCTGCGGATCTCAAAAAAGCG
GGAATTGAATATTCGTATTATCTAATCGATGACGCGGTCGCTCAGCTGATGAAAAATGAAGGTGGAATGCTCTGGGCCCT
GATGAATTACGACGGAGATGTTATGAGCGACATGGTCGCCTCCGGATTTGGATCTTTAGGACTTATGACTTCTGTGCTCG
TTTCTCCTGATGGAAAATTTGAATACGAAGCAGCACACGGTACTGTGACCAGACATTATCGTAAATACCAACAGGGTGAA
ACTACTTCTACAAATTCGGTAGCTTCTATTTTTGCTTGGACGGGTGCGTTGATTAAAAGGGGAGAGTTGGACGGAACTCC
CGATGTGGTCGCATTTGGTCATAAGCTGGAAAAAGCGGTGATTGATACAATCGAAAGCGGAGAGATGACAAAAGATTTAA
CTCTTCTTTGCACCGATTCGAATGCAAAAGCTTTAGACACTTTTCAATTTATGGAAGCAATTCAAAAACGACTTTAA

Upstream 100 bases:

>100_bases
TTGCGGAGAATCCGAATTTTCCTTCCCAATAATCGTCAGGACTTTAGAACAGAATTATATTCTATTATTATATCATTGTA
AAATATTAAAGGAGAATTCA

Downstream 100 bases:

>100_bases
TCTATCGTATTAAAACAAAATTCCCGGCGGGGTACTGTCGGGAATTTAAGATTGATTCGTATAACTAGTGTAATTCGAAA
TTTTAATCGTAAAGATGAAA

Product: isocitrate dehydrogenase

Products: NA

Alternate protein names: IDH; IDP; NADP(+)-specific ICDH; Oxalosuccinate decarboxylase [H]

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MAKIKVKTPLVELDGDEMTRIIWKEIKDRFIHPYLDIELDYYDLGVEYRDKTDDKVTVDSAHAIQKYGVGVKCATITPNQ
DRVKEYNLKQEWKSPNGTIRSILDGTVFRKPIIVKNIPPAVRSWKKPITVGRHAYGDLYKDTELYIPEAGKVELVYTGKD
GKEKQRALIHDFEGAGVIMGQHNLDKSILSFAQACFNYAISEKIDLWFATKDTISKKYHARFRAIFDELAKAKAADLKKA
GIEYSYYLIDDAVAQLMKNEGGMLWALMNYDGDVMSDMVASGFGSLGLMTSVLVSPDGKFEYEAAHGTVTRHYRKYQQGE
TTSTNSVASIFAWTGALIKRGELDGTPDVVAFGHKLEKAVIDTIESGEMTKDLTLLCTDSNAKALDTFQFMEAIQKRL

Sequences:

>Translated_398_residues
MAKIKVKTPLVELDGDEMTRIIWKEIKDRFIHPYLDIELDYYDLGVEYRDKTDDKVTVDSAHAIQKYGVGVKCATITPNQ
DRVKEYNLKQEWKSPNGTIRSILDGTVFRKPIIVKNIPPAVRSWKKPITVGRHAYGDLYKDTELYIPEAGKVELVYTGKD
GKEKQRALIHDFEGAGVIMGQHNLDKSILSFAQACFNYAISEKIDLWFATKDTISKKYHARFRAIFDELAKAKAADLKKA
GIEYSYYLIDDAVAQLMKNEGGMLWALMNYDGDVMSDMVASGFGSLGLMTSVLVSPDGKFEYEAAHGTVTRHYRKYQQGE
TTSTNSVASIFAWTGALIKRGELDGTPDVVAFGHKLEKAVIDTIESGEMTKDLTLLCTDSNAKALDTFQFMEAIQKRL
>Mature_397_residues
AKIKVKTPLVELDGDEMTRIIWKEIKDRFIHPYLDIELDYYDLGVEYRDKTDDKVTVDSAHAIQKYGVGVKCATITPNQD
RVKEYNLKQEWKSPNGTIRSILDGTVFRKPIIVKNIPPAVRSWKKPITVGRHAYGDLYKDTELYIPEAGKVELVYTGKDG
KEKQRALIHDFEGAGVIMGQHNLDKSILSFAQACFNYAISEKIDLWFATKDTISKKYHARFRAIFDELAKAKAADLKKAG
IEYSYYLIDDAVAQLMKNEGGMLWALMNYDGDVMSDMVASGFGSLGLMTSVLVSPDGKFEYEAAHGTVTRHYRKYQQGET
TSTNSVASIFAWTGALIKRGELDGTPDVVAFGHKLEKAVIDTIESGEMTKDLTLLCTDSNAKALDTFQFMEAIQKRL

Specific function: Catalyzes The Oxidation Of 3-Carboxy-2-Hydroxy-4- Methylpentanoate (3-Isopropylmalate) To 3-Carboxy-4-Methyl-2- Oxopentanoate. The Product Decarboxylates To 4-Methyl-2 Oxopentanoate. [C]

COG id: COG0538

COG function: function code C; Isocitrate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI28178832, Length=403, Percent_Identity=55.8312655086849, Blast_Score=449, Evalue=1e-126,
Organism=Homo sapiens, GI28178825, Length=396, Percent_Identity=55.5555555555556, Blast_Score=436, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI17550822, Length=405, Percent_Identity=54.8148148148148, Blast_Score=442, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI17540874, Length=396, Percent_Identity=53.5353535353535, Blast_Score=439, Evalue=1e-123,
Organism=Saccharomyces cerevisiae, GI6320137, Length=404, Percent_Identity=57.6732673267327, Blast_Score=475, Evalue=1e-135,
Organism=Saccharomyces cerevisiae, GI6323203, Length=403, Percent_Identity=56.3275434243176, Blast_Score=455, Evalue=1e-129,
Organism=Saccharomyces cerevisiae, GI6324319, Length=403, Percent_Identity=55.0868486352357, Blast_Score=454, Evalue=1e-128,
Organism=Drosophila melanogaster, GI21356561, Length=398, Percent_Identity=55.2763819095477, Blast_Score=439, Evalue=1e-123,
Organism=Drosophila melanogaster, GI221330987, Length=398, Percent_Identity=55.2763819095477, Blast_Score=439, Evalue=1e-123,
Organism=Drosophila melanogaster, GI221330985, Length=398, Percent_Identity=55.2763819095477, Blast_Score=439, Evalue=1e-123,
Organism=Drosophila melanogaster, GI28574947, Length=398, Percent_Identity=55.2763819095477, Blast_Score=439, Evalue=1e-123,
Organism=Drosophila melanogaster, GI24660849, Length=398, Percent_Identity=55.2763819095477, Blast_Score=439, Evalue=1e-123,
Organism=Drosophila melanogaster, GI24660856, Length=398, Percent_Identity=55.2763819095477, Blast_Score=438, Evalue=1e-123,
Organism=Drosophila melanogaster, GI221330983, Length=398, Percent_Identity=55.2763819095477, Blast_Score=437, Evalue=1e-123,
Organism=Drosophila melanogaster, GI24660868, Length=398, Percent_Identity=55.2763819095477, Blast_Score=437, Evalue=1e-123,
Organism=Drosophila melanogaster, GI24660860, Length=398, Percent_Identity=55.2763819095477, Blast_Score=437, Evalue=1e-123,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004790 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.42 [H]

Molecular weight: Translated: 44763; Mature: 44631

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKIKVKTPLVELDGDEMTRIIWKEIKDRFIHPYLDIELDYYDLGVEYRDKTDDKVTVDS
CCEEEEECCEEEECCHHHHHHHHHHHHHHHCCCEEEEEEEEEECCEEECCCCCCEEEECH
AHAIQKYGVGVKCATITPNQDRVKEYNLKQEWKSPNGTIRSILDGTVFRKPIIVKNIPPA
HHHHHHHCCCEEEEEECCCHHHHHHCCCHHHHCCCCCHHHHHHCCHHHCCCEEECCCCHH
VRSWKKPITVGRHAYGDLYKDTELYIPEAGKVELVYTGKDGKEKQRALIHDFEGAGVIMG
HHHCCCCCCCCCHHHCCCCCCCEEECCCCCCEEEEEECCCCCHHHHHHHCCCCCCEEEEE
QHNLDKSILSFAQACFNYAISEKIDLWFATKDTISKKYHARFRAIFDELAKAKAADLKKA
CCCCHHHHHHHHHHHHHHHHHCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GIEYSYYLIDDAVAQLMKNEGGMLWALMNYDGDVMSDMVASGFGSLGLMTSVLVSPDGKF
CCCEEEEEHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCE
EYEAAHGTVTRHYRKYQQGETTSTNSVASIFAWTGALIKRGELDGTPDVVAFGHKLEKAV
EEEECCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
IDTIESGEMTKDLTLLCTDSNAKALDTFQFMEAIQKRL
HHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AKIKVKTPLVELDGDEMTRIIWKEIKDRFIHPYLDIELDYYDLGVEYRDKTDDKVTVDS
CEEEEECCEEEECCHHHHHHHHHHHHHHHCCCEEEEEEEEEECCEEECCCCCCEEEECH
AHAIQKYGVGVKCATITPNQDRVKEYNLKQEWKSPNGTIRSILDGTVFRKPIIVKNIPPA
HHHHHHHCCCEEEEEECCCHHHHHHCCCHHHHCCCCCHHHHHHCCHHHCCCEEECCCCHH
VRSWKKPITVGRHAYGDLYKDTELYIPEAGKVELVYTGKDGKEKQRALIHDFEGAGVIMG
HHHCCCCCCCCCHHHCCCCCCCEEECCCCCCEEEEEECCCCCHHHHHHHCCCCCCEEEEE
QHNLDKSILSFAQACFNYAISEKIDLWFATKDTISKKYHARFRAIFDELAKAKAADLKKA
CCCCHHHHHHHHHHHHHHHHHCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GIEYSYYLIDDAVAQLMKNEGGMLWALMNYDGDVMSDMVASGFGSLGLMTSVLVSPDGKF
CCCEEEEEHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCE
EYEAAHGTVTRHYRKYQQGETTSTNSVASIFAWTGALIKRGELDGTPDVVAFGHKLEKAV
EEEECCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
IDTIESGEMTKDLTLLCTDSNAKALDTFQFMEAIQKRL
HHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8626059 [H]