BacMap

Definition	Leptospira interrogans serovar Copenhageni str. Fiocruz L1-130 chromosome chromosome I, complete sequence.
Accession	NC_005823
Length	4,277,185

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The map label for this gene is hetN [H]

Identifier: 45659054

GI number: 45659054

Start: 3968900

End: 3969700

Strand: Reverse

Name: hetN [H]

Synonym: LIC13232

Alternate gene names: 45659054

Gene position: 3969700-3968900 (Counterclockwise)

Preceding gene: 45659058

Following gene: 45659048

Centisome position: 92.81

GC content: 38.7

Gene sequence:

>801_bases
ATGATTCATTCTTTCTTTTTAGAAAAATCTTTTTTGGTTACGGGAGCTAGTTCCGGTATTGGTAGGGCTCTTGTTTTAGA
ACTCAATCGAAATGGTGCTGTAGTAGGGGCAATTGCTCGAAGAAAAGAATTGTTAAAAGAGTTAAAAAGTGAAGCTAAGT
TTCCAGATAAAATCATTTCTTTTCCCGGAGACGTTTCTGATCTTTCTCAGCTCAAAAAGATTACAGAGGAATTCAGAAAA
AAAGTTAGGCGTATCGATGGAATGATTCATAGCGCTGGAATTAGCATGCGTGGTCTTGCCAAAGAGACGGATATTAAAGT
TTATGAAAATCTAATGAACATAAATTTTTATCCTTTGGTTCATTTATTCAAACTTTTAGAATCGGAACTGAGGCAGAATC
AAGGTCATTTTATTGCTGTATCTTCTTTGCAAGGAAGATTTGCAACTCAGTATCGTTCTGGTTATGCTGCGAGCAAACAC
GCAGTTCAGGCGTTTATGGATAGTATTCGACTGGAAACCTGCGAGAGTGGGATGCACGTTATGACGGTTTCTCCGGGTTA
TGTAAAAACGGATATTTCTTTGAGAGCTTTATCTGGAGACGGTTCCGCTTACGGAATTATGGATGAAGGAATTAAGAACG
GACTTGCGACAGAAAAAGTGGCGACCATCATTTTGAAGGCCATTGAATCCAGAAAGAGAGACGTATACCCGTCTCAGTTC
AGAGAGATGTTGGCTTATTGGATCAGTCGATTTTCTCCTTCTTTATTAGATCGACTTTTGAGAAATGCAAGAGTTACTTA
G

Upstream 100 bases:

>100_bases
TTTCAGGATCATCTTTTCACTAGACTCAATTCTCTAAAGAATTTTTCCGTTTATTTTCATTTGCTTTTTTAAGACTTTCA
AATATCTTCGGTCCGGTCTT

Downstream 100 bases:

>100_bases
TATTTCCTTCACAATCTGGTAATCAAGAGTTTCGTATTGTGTAAATTCTACTTAAAATTTTTAGATACTCCAACGCCGGC
TCTTAGTTAACTTGAATTGG

Product: short-chain dehydrogenase

Products: 3-oxoacyl-[acyl-carrier protein]; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MIHSFFLEKSFLVTGASSGIGRALVLELNRNGAVVGAIARRKELLKELKSEAKFPDKIISFPGDVSDLSQLKKITEEFRK
KVRRIDGMIHSAGISMRGLAKETDIKVYENLMNINFYPLVHLFKLLESELRQNQGHFIAVSSLQGRFATQYRSGYAASKH
AVQAFMDSIRLETCESGMHVMTVSPGYVKTDISLRALSGDGSAYGIMDEGIKNGLATEKVATIILKAIESRKRDVYPSQF
REMLAYWISRFSPSLLDRLLRNARVT

Sequences:

>Translated_266_residues
MIHSFFLEKSFLVTGASSGIGRALVLELNRNGAVVGAIARRKELLKELKSEAKFPDKIISFPGDVSDLSQLKKITEEFRK
KVRRIDGMIHSAGISMRGLAKETDIKVYENLMNINFYPLVHLFKLLESELRQNQGHFIAVSSLQGRFATQYRSGYAASKH
AVQAFMDSIRLETCESGMHVMTVSPGYVKTDISLRALSGDGSAYGIMDEGIKNGLATEKVATIILKAIESRKRDVYPSQF
REMLAYWISRFSPSLLDRLLRNARVT
>Mature_266_residues
MIHSFFLEKSFLVTGASSGIGRALVLELNRNGAVVGAIARRKELLKELKSEAKFPDKIISFPGDVSDLSQLKKITEEFRK
KVRRIDGMIHSAGISMRGLAKETDIKVYENLMNINFYPLVHLFKLLESELRQNQGHFIAVSSLQGRFATQYRSGYAASKH
AVQAFMDSIRLETCESGMHVMTVSPGYVKTDISLRALSGDGSAYGIMDEGIKNGLATEKVATIILKAIESRKRDVYPSQF
REMLAYWISRFSPSLLDRLLRNARVT

Specific function: May be involved in repressing heterocyst differentiation and may be essential for preventing all vegetative cells from differentiating [H]

COG id: COG1028

COG function: function code IQR; Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI20149619, Length=266, Percent_Identity=33.8345864661654, Blast_Score=147, Evalue=7e-36,
Organism=Homo sapiens, GI7706318, Length=249, Percent_Identity=27.3092369477912, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI157739938, Length=258, Percent_Identity=23.2558139534884, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI4503817, Length=181, Percent_Identity=25.414364640884, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI44680136, Length=186, Percent_Identity=30.1075268817204, Blast_Score=74, Evalue=9e-14,
Organism=Homo sapiens, GI44680133, Length=186, Percent_Identity=30.1075268817204, Blast_Score=74, Evalue=9e-14,
Organism=Homo sapiens, GI17738292, Length=186, Percent_Identity=30.1075268817204, Blast_Score=74, Evalue=9e-14,
Organism=Homo sapiens, GI150247226, Length=195, Percent_Identity=26.6666666666667, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI40548397, Length=191, Percent_Identity=27.7486910994764, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI214010158, Length=191, Percent_Identity=27.7486910994764, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI214010156, Length=191, Percent_Identity=27.7486910994764, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI22758144, Length=191, Percent_Identity=27.7486910994764, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI19743808, Length=193, Percent_Identity=25.9067357512953, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI109715829, Length=224, Percent_Identity=25.8928571428571, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI40807363, Length=221, Percent_Identity=25.7918552036199, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI32455239, Length=197, Percent_Identity=30.9644670050761, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI5031765, Length=197, Percent_Identity=30.9644670050761, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI7657478, Length=190, Percent_Identity=25.7894736842105, Blast_Score=68, Evalue=8e-12,
Organism=Homo sapiens, GI40254992, Length=188, Percent_Identity=26.063829787234, Blast_Score=65, Evalue=4e-11,
Organism=Escherichia coli, GI1786701, Length=189, Percent_Identity=30.6878306878307, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787335, Length=196, Percent_Identity=27.5510204081633, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1786812, Length=188, Percent_Identity=27.1276595744681, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1787820, Length=193, Percent_Identity=26.9430051813472, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1790717, Length=197, Percent_Identity=25.8883248730964, Blast_Score=61, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI71997402, Length=257, Percent_Identity=35.7976653696498, Blast_Score=152, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17570069, Length=258, Percent_Identity=33.7209302325581, Blast_Score=139, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI71994604, Length=191, Percent_Identity=32.9842931937173, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17563726, Length=193, Percent_Identity=31.6062176165803, Blast_Score=85, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI71994600, Length=198, Percent_Identity=31.8181818181818, Blast_Score=85, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17560220, Length=195, Percent_Identity=32.3076923076923, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI115534660, Length=188, Percent_Identity=27.6595744680851, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI193210355, Length=214, Percent_Identity=26.6355140186916, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17531453, Length=196, Percent_Identity=30.6122448979592, Blast_Score=77, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17562908, Length=199, Percent_Identity=29.6482412060301, Blast_Score=76, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17560150, Length=194, Percent_Identity=28.3505154639175, Blast_Score=76, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17564282, Length=187, Percent_Identity=29.9465240641711, Blast_Score=75, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17562906, Length=194, Percent_Identity=29.8969072164948, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI72000259, Length=196, Percent_Identity=29.0816326530612, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17562990, Length=195, Percent_Identity=27.6923076923077, Blast_Score=74, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17550780, Length=106, Percent_Identity=37.7358490566038, Blast_Score=74, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17551412, Length=204, Percent_Identity=27.9411764705882, Blast_Score=74, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17557780, Length=230, Percent_Identity=25.2173913043478, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI32563809, Length=193, Percent_Identity=25.3886010362694, Blast_Score=70, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17507613, Length=193, Percent_Identity=25.3886010362694, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17559104, Length=213, Percent_Identity=26.7605633802817, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17508895, Length=247, Percent_Identity=25.5060728744939, Blast_Score=68, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17562910, Length=195, Percent_Identity=26.6666666666667, Blast_Score=68, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI193203115, Length=192, Percent_Identity=26.5625, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17560332, Length=199, Percent_Identity=26.1306532663317, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI193203117, Length=192, Percent_Identity=26.5625, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17538480, Length=196, Percent_Identity=27.0408163265306, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17562904, Length=195, Percent_Identity=26.6666666666667, Blast_Score=64, Evalue=1e-10,
Organism=Saccharomyces cerevisiae, GI6323882, Length=264, Percent_Identity=29.9242424242424, Blast_Score=83, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6322226, Length=192, Percent_Identity=30.2083333333333, Blast_Score=82, Evalue=9e-17,
Organism=Saccharomyces cerevisiae, GI6322227, Length=193, Percent_Identity=30.5699481865285, Blast_Score=77, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6322067, Length=184, Percent_Identity=28.2608695652174, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI21358495, Length=254, Percent_Identity=38.1889763779528, Blast_Score=163, Evalue=1e-40,
Organism=Drosophila melanogaster, GI28571526, Length=200, Percent_Identity=33, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24580925, Length=265, Percent_Identity=28.3018867924528, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24644339, Length=194, Percent_Identity=30.4123711340206, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI21357041, Length=193, Percent_Identity=29.5336787564767, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI23397609, Length=186, Percent_Identity=31.1827956989247, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24648944, Length=195, Percent_Identity=28.7179487179487, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI116007236, Length=196, Percent_Identity=28.0612244897959, Blast_Score=74, Evalue=9e-14,
Organism=Drosophila melanogaster, GI21355631, Length=262, Percent_Identity=27.0992366412214, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21356301, Length=181, Percent_Identity=27.6243093922652, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24648693, Length=206, Percent_Identity=28.1553398058252, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI21355889, Length=206, Percent_Identity=28.1553398058252, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24641232, Length=194, Percent_Identity=29.3814432989691, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI28571387, Length=193, Percent_Identity=30.5699481865285, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI116007234, Length=221, Percent_Identity=26.2443438914027, Blast_Score=67, Evalue=8e-12,
Organism=Drosophila melanogaster, GI116007238, Length=174, Percent_Identity=28.1609195402299, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24641388, Length=204, Percent_Identity=29.9019607843137, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24649181, Length=208, Percent_Identity=25.4807692307692, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.100

Molecular weight: Translated: 29812; Mature: 29812

Theoretical pI: Translated: 10.26; Mature: 10.26

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIHSFFLEKSFLVTGASSGIGRALVLELNRNGAVVGAIARRKELLKELKSEAKFPDKIIS
CCCHHHHHHHHEEECCCCCCCEEEEEEECCCCCEEHHHHHHHHHHHHHHHHCCCCHHHHC
FPGDVSDLSQLKKITEEFRKKVRRIDGMIHSAGISMRGLAKETDIKVYENLMNINFYPLV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCHHHHHHHHHHHCCCHHHHH
HLFKLLESELRQNQGHFIAVSSLQGRFATQYRSGYAASKHAVQAFMDSIRLETCESGMHV
HHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEE
MTVSPGYVKTDISLRALSGDGSAYGIMDEGIKNGLATEKVATIILKAIESRKRDVYPSQF
EEECCCEEEECCEEEEECCCCCEECCHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHH
REMLAYWISRFSPSLLDRLLRNARVT
HHHHHHHHHHCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MIHSFFLEKSFLVTGASSGIGRALVLELNRNGAVVGAIARRKELLKELKSEAKFPDKIIS
CCCHHHHHHHHEEECCCCCCCEEEEEEECCCCCEEHHHHHHHHHHHHHHHHCCCCHHHHC
FPGDVSDLSQLKKITEEFRKKVRRIDGMIHSAGISMRGLAKETDIKVYENLMNINFYPLV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCHHHHHHHHHHHCCCHHHHH
HLFKLLESELRQNQGHFIAVSSLQGRFATQYRSGYAASKHAVQAFMDSIRLETCESGMHV
HHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEE
MTVSPGYVKTDISLRALSGDGSAYGIMDEGIKNGLATEKVATIILKAIESRKRDVYPSQF
EEECCCEEEECCEEEEECCCCCEECCHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHH
REMLAYWISRFSPSLLDRLLRNARVT
HHHHHHHHHHCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (3R)-3-hydroxyacyl-[acyl-carrier protein]; NADP+

Specific reaction: (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+ = 3-oxoacyl-[acyl-carrier protein] + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8157596; 11759840 [H]