Click here to switch to the map view.

The map label for this gene is eno

Identifier: 45657813

GI number: 45657813

Start: 2360699

End: 2361997

Strand: Reverse

Name: eno

Synonym: LIC11954

Alternate gene names: 45657813

Gene position: 2361997-2360699 (Counterclockwise)

Preceding gene: 45657828

Following gene: 45657812

Centisome position: 55.22

GC content: 40.34

Gene sequence:

>1299_bases
ATGTCTCATCACTCTCAAATTCAGAAAATTCAAGCTCGGGAAATTATGGACTCCCGTGGAAATCCTACGGTCGAAGTGGA
CGTAATCCTTTTAGATGGTTCTTTTGGAAGAGCGGCGGTTCCTTCCGGAGCGTCTACTGGAGAATACGAAGCCGTAGAAC
TTAGAGACGGAGATAAACATCGTTATCTTGGAAAAGGAGTACTTAAGGCGGTAGAACACGTAAACCTGAAAATTCAGGAA
GTATTGAAAGGAGAAAATGCGATCGATCAAAATAGAATCGACCAACTCATGTTAGACGCTGATGGAACCAAAAACAAAGG
TAAACTAGGCGCGAATGCAATTTTAGGAACTTCTCTCGCGGTAGCCAAGGCTGCTGCTGCTCATTCTAAACTTCCATTAT
ATCGTTATATAGGAGGAAACTTTGCCAGAGAACTACCCGTTCCTATGATGAATATCATCAACGGAGGCGCTCATGCGGAT
AACAACGTAGACTTTCAAGAATTTATGATTCTTCCCGTAGGAGCCAAAAGTTTTCGAGAAGCTCTTCGTATGGGGGCGGA
AATATTTCATTCTTTAAAATCAGTACTCAAAGGAAAGAAATTGAACACTGCCGTCGGAGATGAAGGTGGATTTGCTCCAG
ATCTGACTAGTAATGTGGAAGCGATCGAAGTTATTCTACAAGCTATTGAAAAAGCAGGATATAAACCTGAAAAAGACGTT
TTACTCGGTTTGGATGCGGCTTCTTCCGAATTTTATGACAAAAGTAAAAAGAAATACGTACTCGGTGCCGAAAATAATAA
AGAGTTCTCCAGTGCCGAACTCGTGGATTATTATGCGAATTTGGTTTCCAAATATCCGATTATTACGATCGAAGACGGTT
TGGACGAAAATGACTGGGATGGTTGGAAACTTCTTTCCGAAAAATTAGGAAAGAAAATTCAGCTCGTAGGAGACGACCTT
TTTGTGACAAATATAGAGAAACTCTCCAAAGGAATTTCTTCCGGAGTTGGAAATTCGATTCTGATCAAGGTAAATCAGAT
CGGTTCTCTTTCTGAAACTCTCTCTTCTATCGAAATGGCGAAAAAAGCGAAATATACAAACGTCGTAAGCCATAGAAGTG
GGGAAACGGAAGACGTTACGATTTCTCATATTGCAGTTGCGACTAACGCAGGACAGATTAAAACCGGTTCTCTTTCTAGA
ACGGATAGAATTGCAAAATATAACGAACTTCTAAGGATCGAAGAAGAACTCGGAAAGTCAGCAGTTTATAAGGGTAGGGA
AACTTTTTATAATTTATAA

Upstream 100 bases:

>100_bases
ACGTTCTAAATTGTTTTTCTTTGGAAGTAAAAATCGGTTTTTTGTTTTACAACCTTTTCAACTCAAGCAGACAAAACATA
CATAAATTTCAGGAAATAAA

Downstream 100 bases:

>100_bases
AATTGTTTTTGTATTTGGAAAAAACGCGTCTTAGTTTAAAATAGACGCGTCTAAAGCAAACAAATACAAGTTTCTATAAA
ACAGATTTGCGATTTTTATT

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase [H]

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MSHHSQIQKIQAREIMDSRGNPTVEVDVILLDGSFGRAAVPSGASTGEYEAVELRDGDKHRYLGKGVLKAVEHVNLKIQE
VLKGENAIDQNRIDQLMLDADGTKNKGKLGANAILGTSLAVAKAAAAHSKLPLYRYIGGNFARELPVPMMNIINGGAHAD
NNVDFQEFMILPVGAKSFREALRMGAEIFHSLKSVLKGKKLNTAVGDEGGFAPDLTSNVEAIEVILQAIEKAGYKPEKDV
LLGLDAASSEFYDKSKKKYVLGAENNKEFSSAELVDYYANLVSKYPIITIEDGLDENDWDGWKLLSEKLGKKIQLVGDDL
FVTNIEKLSKGISSGVGNSILIKVNQIGSLSETLSSIEMAKKAKYTNVVSHRSGETEDVTISHIAVATNAGQIKTGSLSR
TDRIAKYNELLRIEEELGKSAVYKGRETFYNL

Sequences:

>Translated_432_residues
MSHHSQIQKIQAREIMDSRGNPTVEVDVILLDGSFGRAAVPSGASTGEYEAVELRDGDKHRYLGKGVLKAVEHVNLKIQE
VLKGENAIDQNRIDQLMLDADGTKNKGKLGANAILGTSLAVAKAAAAHSKLPLYRYIGGNFARELPVPMMNIINGGAHAD
NNVDFQEFMILPVGAKSFREALRMGAEIFHSLKSVLKGKKLNTAVGDEGGFAPDLTSNVEAIEVILQAIEKAGYKPEKDV
LLGLDAASSEFYDKSKKKYVLGAENNKEFSSAELVDYYANLVSKYPIITIEDGLDENDWDGWKLLSEKLGKKIQLVGDDL
FVTNIEKLSKGISSGVGNSILIKVNQIGSLSETLSSIEMAKKAKYTNVVSHRSGETEDVTISHIAVATNAGQIKTGSLSR
TDRIAKYNELLRIEEELGKSAVYKGRETFYNL
>Mature_431_residues
SHHSQIQKIQAREIMDSRGNPTVEVDVILLDGSFGRAAVPSGASTGEYEAVELRDGDKHRYLGKGVLKAVEHVNLKIQEV
LKGENAIDQNRIDQLMLDADGTKNKGKLGANAILGTSLAVAKAAAAHSKLPLYRYIGGNFARELPVPMMNIINGGAHADN
NVDFQEFMILPVGAKSFREALRMGAEIFHSLKSVLKGKKLNTAVGDEGGFAPDLTSNVEAIEVILQAIEKAGYKPEKDVL
LGLDAASSEFYDKSKKKYVLGAENNKEFSSAELVDYYANLVSKYPIITIEDGLDENDWDGWKLLSEKLGKKIQLVGDDLF
VTNIEKLSKGISSGVGNSILIKVNQIGSLSETLSSIEMAKKAKYTNVVSHRSGETEDVTISHIAVATNAGQIKTGSLSRT
DRIAKYNELLRIEEELGKSAVYKGRETFYNL

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI5803011, Length=428, Percent_Identity=52.3364485981308, Blast_Score=437, Evalue=1e-122,
Organism=Homo sapiens, GI4503571, Length=429, Percent_Identity=52.4475524475524, Blast_Score=430, Evalue=1e-120,
Organism=Homo sapiens, GI301897477, Length=431, Percent_Identity=51.9721577726218, Blast_Score=428, Evalue=1e-120,
Organism=Homo sapiens, GI301897469, Length=431, Percent_Identity=51.9721577726218, Blast_Score=428, Evalue=1e-120,
Organism=Homo sapiens, GI301897479, Length=429, Percent_Identity=47.7855477855478, Blast_Score=373, Evalue=1e-103,
Organism=Homo sapiens, GI169201331, Length=354, Percent_Identity=26.5536723163842, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI169201757, Length=354, Percent_Identity=26.5536723163842, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI239744207, Length=354, Percent_Identity=26.5536723163842, Blast_Score=100, Evalue=4e-21,
Organism=Escherichia coli, GI1789141, Length=427, Percent_Identity=62.0608899297424, Blast_Score=510, Evalue=1e-146,
Organism=Caenorhabditis elegans, GI71995829, Length=430, Percent_Identity=51.3953488372093, Blast_Score=414, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI17536383, Length=430, Percent_Identity=51.3953488372093, Blast_Score=414, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI32563855, Length=190, Percent_Identity=42.1052631578947, Blast_Score=164, Evalue=7e-41,
Organism=Saccharomyces cerevisiae, GI6324974, Length=432, Percent_Identity=47.9166666666667, Blast_Score=380, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6324969, Length=432, Percent_Identity=47.9166666666667, Blast_Score=380, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6323985, Length=432, Percent_Identity=47.6851851851852, Blast_Score=379, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6321693, Length=440, Percent_Identity=46.1363636363636, Blast_Score=364, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6321968, Length=440, Percent_Identity=46.3636363636364, Blast_Score=348, Evalue=8e-97,
Organism=Drosophila melanogaster, GI24580918, Length=437, Percent_Identity=49.6567505720824, Blast_Score=383, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580916, Length=437, Percent_Identity=49.6567505720824, Blast_Score=383, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580920, Length=437, Percent_Identity=49.6567505720824, Blast_Score=383, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580914, Length=437, Percent_Identity=49.6567505720824, Blast_Score=383, Evalue=1e-106,
Organism=Drosophila melanogaster, GI281360527, Length=432, Percent_Identity=50, Blast_Score=380, Evalue=1e-106,
Organism=Drosophila melanogaster, GI17137654, Length=432, Percent_Identity=50, Blast_Score=380, Evalue=1e-106,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 47045; Mature: 46914

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS00164 ENOLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHHSQIQKIQAREIMDSRGNPTVEVDVILLDGSFGRAAVPSGASTGEYEAVELRDGDKH
CCCHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCH
RYLGKGVLKAVEHVNLKIQEVLKGENAIDQNRIDQLMLDADGTKNKGKLGANAILGTSLA
HHHHHHHHHHHHHCCCHHHHHHCCCCCCCHHHHHHEEECCCCCCCCCCCCCHHHHHHHHH
VAKAAAAHSKLPLYRYIGGNFARELPVPMMNIINGGAHADNNVDFQEFMILPVGAKSFRE
HHHHHHHHCCCCCHHHHCCCHHHHCCCHHHHHHCCCCCCCCCCCHHHEEEEECCHHHHHH
ALRMGAEIFHSLKSVLKGKKLNTAVGDEGGFAPDLTSNVEAIEVILQAIEKAGYKPEKDV
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCE
LLGLDAASSEFYDKSKKKYVLGAENNKEFSSAELVDYYANLVSKYPIITIEDGLDENDWD
EEECCCCCHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCH
GWKLLSEKLGKKIQLVGDDLFVTNIEKLSKGISSGVGNSILIKVNQIGSLSETLSSIEMA
HHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHH
KKAKYTNVVSHRSGETEDVTISHIAVATNAGQIKTGSLSRTDRIAKYNELLRIEEELGKS
HHHHHHHHHHCCCCCCCCEEEEEEEEEECCCCEECCCCCHHHHHHHHHHHHHHHHHHCHH
AVYKGRETFYNL
HHHCCHHHHCCC
>Mature Secondary Structure 
SHHSQIQKIQAREIMDSRGNPTVEVDVILLDGSFGRAAVPSGASTGEYEAVELRDGDKH
CCHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCH
RYLGKGVLKAVEHVNLKIQEVLKGENAIDQNRIDQLMLDADGTKNKGKLGANAILGTSLA
HHHHHHHHHHHHHCCCHHHHHHCCCCCCCHHHHHHEEECCCCCCCCCCCCCHHHHHHHHH
VAKAAAAHSKLPLYRYIGGNFARELPVPMMNIINGGAHADNNVDFQEFMILPVGAKSFRE
HHHHHHHHCCCCCHHHHCCCHHHHCCCHHHHHHCCCCCCCCCCCHHHEEEEECCHHHHHH
ALRMGAEIFHSLKSVLKGKKLNTAVGDEGGFAPDLTSNVEAIEVILQAIEKAGYKPEKDV
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCE
LLGLDAASSEFYDKSKKKYVLGAENNKEFSSAELVDYYANLVSKYPIITIEDGLDENDWD
EEECCCCCHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCH
GWKLLSEKLGKKIQLVGDDLFVTNIEKLSKGISSGVGNSILIKVNQIGSLSETLSSIEMA
HHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHH
KKAKYTNVVSHRSGETEDVTISHIAVATNAGQIKTGSLSRTDRIAKYNELLRIEEELGKS
HHHHHHHHHHCCCCCCCCEEEEEEEEEECCCCEECCCCCHHHHHHHHHHHHHHHHHHCHH
AVYKGRETFYNL
HHHCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA