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The map label for this gene is ddlA

Identifier: 45657718

GI number: 45657718

Start: 2250057

End: 2251112

Strand: Reverse

Name: ddlA

Synonym: LIC11855

Alternate gene names: 45657718

Gene position: 2251112-2250057 (Counterclockwise)

Preceding gene: 45657724

Following gene: 45657714

Centisome position: 52.63

GC content: 37.97

Gene sequence:

>1056_bases
TTGGCAAAGATTGCAGTCTTTTTTGGTGGTAGTTCTACGGAACACAGTATTTCGATTCTAACCGGATGTTTTATTTGTAA
GACATTGCATACAATGGGACATTCGGTCAAACCCATTCTTTTAACCAAGGATGGGGGTTGGGTCGTCCCTTCTGAATATC
GGATGTCGATTCCGTTTGAAGTGTCTAATTCTCCAGATTTATTTCAGGAAGAATTTCAAAAAAGATACGGAGTGTCCAGA
ACGAATCAAATTTTTTCTTTGGATGCGGATATCGCTTTTTTAGGTCTTCACGGAGGCCAGGGAGAAGACGGAACGATCCA
AGGGTTTTTAGAAATTTTAGGAATTCCTTATACTGGTTCTGGAGTTCTTGCTTCTGCGATTGCAATGGATAAAACCAGAG
CCAATCAGATATTTTTACAATCCGGTCAGAAGGTTGCACCTTTTTTCGAAATTGATAAATTAGAATATCTAAATTCTACC
GATGCAGTGATTACAAAATTAGAAACCCTAGGTTTTCCACAATTTTTAAAACCAGTGGAAGGTGGTTCGAGCGTCTCTGT
TTATAAAATAACAAATAGGGAACAGCTGAAAGAAAAACTTGCTTTAATTTTTGAATCCGATTCTAAAGTGATGAGTCAGT
CTTTTTTGACTGGCATTGAGGTTTCTTGTGGGGTTTTAGAAAGATACAGAGACGGAAAATTCAAAAAGATTGCGTTACCC
GCAACTGAAATCGTTCCTGGGGGAGAATTTTTTGACTTCGAATCTAAATACAAACAAGGAGGTTCTCATGAAATCACACC
TGCTAGAATTTCGGAACAAGAAATGAAACGTGTTCAAGAACTTGCTATAGCTGCACATAGATCTTTAGGATGTAGCGGAT
ATTCTAGAACCGATTTTATTATCGTAAACGGAGAACCTCATATTTTAGAGACTAACACGTTGCCTGGAATGACGGAAACG
AGTCTAATTCCTCAACAAGCCAAAGCGGCTGGAATTTCTATGGAAGAAGTTTTTTCCGATCTAATAGAAATAGGACTAAA
ACGTTCTCTTTATTAG

Upstream 100 bases:

>100_bases
ATTCTCCCACAGGTTTAGAAGGATATTCTGAAAATATGTTTTCAGAGTAGAAAAGTATAATTACGATGCAAAAGATTCTA
TAGAAATCGGAGAATTTTTT

Downstream 100 bases:

>100_bases
AAAATTAGGATATTACGAAAAGCGCAAGCTTAGAAAAGAAGTTGGGGCGAAAGCGAACCTGGGATAAATCTCTGAAGTAA
GCCCTGTCTTCGATCTTAAA

Product: D-alanyl-alanine synthetase A

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 351; Mature: 350

Protein sequence:

>351_residues
MAKIAVFFGGSSTEHSISILTGCFICKTLHTMGHSVKPILLTKDGGWVVPSEYRMSIPFEVSNSPDLFQEEFQKRYGVSR
TNQIFSLDADIAFLGLHGGQGEDGTIQGFLEILGIPYTGSGVLASAIAMDKTRANQIFLQSGQKVAPFFEIDKLEYLNST
DAVITKLETLGFPQFLKPVEGGSSVSVYKITNREQLKEKLALIFESDSKVMSQSFLTGIEVSCGVLERYRDGKFKKIALP
ATEIVPGGEFFDFESKYKQGGSHEITPARISEQEMKRVQELAIAAHRSLGCSGYSRTDFIIVNGEPHILETNTLPGMTET
SLIPQQAKAAGISMEEVFSDLIEIGLKRSLY

Sequences:

>Translated_351_residues
MAKIAVFFGGSSTEHSISILTGCFICKTLHTMGHSVKPILLTKDGGWVVPSEYRMSIPFEVSNSPDLFQEEFQKRYGVSR
TNQIFSLDADIAFLGLHGGQGEDGTIQGFLEILGIPYTGSGVLASAIAMDKTRANQIFLQSGQKVAPFFEIDKLEYLNST
DAVITKLETLGFPQFLKPVEGGSSVSVYKITNREQLKEKLALIFESDSKVMSQSFLTGIEVSCGVLERYRDGKFKKIALP
ATEIVPGGEFFDFESKYKQGGSHEITPARISEQEMKRVQELAIAAHRSLGCSGYSRTDFIIVNGEPHILETNTLPGMTET
SLIPQQAKAAGISMEEVFSDLIEIGLKRSLY
>Mature_350_residues
AKIAVFFGGSSTEHSISILTGCFICKTLHTMGHSVKPILLTKDGGWVVPSEYRMSIPFEVSNSPDLFQEEFQKRYGVSRT
NQIFSLDADIAFLGLHGGQGEDGTIQGFLEILGIPYTGSGVLASAIAMDKTRANQIFLQSGQKVAPFFEIDKLEYLNSTD
AVITKLETLGFPQFLKPVEGGSSVSVYKITNREQLKEKLALIFESDSKVMSQSFLTGIEVSCGVLERYRDGKFKKIALPA
TEIVPGGEFFDFESKYKQGGSHEITPARISEQEMKRVQELAIAAHRSLGCSGYSRTDFIIVNGEPHILETNTLPGMTETS
LIPQQAKAAGISMEEVFSDLIEIGLKRSLY

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786280, Length=344, Percent_Identity=31.9767441860465, Blast_Score=188, Evalue=4e-49,
Organism=Escherichia coli, GI1786579, Length=356, Percent_Identity=31.7415730337079, Blast_Score=173, Evalue=2e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_LEPIC (Q72R93)

Other databases:

- EMBL:   AE016823
- RefSeq:   YP_001804.1
- ProteinModelPortal:   Q72R93
- GeneID:   2771229
- GenomeReviews:   AE016823_GR
- KEGG:   lic:LIC11855
- NMPDR:   fig|267671.1.peg.1804
- HOGENOM:   HBG427092
- OMA:   PLSIVPE
- ProtClustDB:   PRK14572
- BioCyc:   LINT267671:LIC_11855-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 38564; Mature: 38432

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKIAVFFGGSSTEHSISILTGCFICKTLHTMGHSVKPILLTKDGGWVVPSEYRMSIPFE
CCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEECCCCCEEECCEE
VSNSPDLFQEEFQKRYGVSRTNQIFSLDADIAFLGLHGGQGEDGTIQGFLEILGIPYTGS
ECCCCHHHHHHHHHHHCCCCCCEEEEECCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCC
GVLASAIAMDKTRANQIFLQSGQKVAPFFEIDKLEYLNSTDAVITKLETLGFPQFLKPVE
HHHHHHHHHHHHHHHHHHHHCCCEECCEEEHHHHHHCCCCHHHHHHHHHCCCHHHHHHCC
GGSSVSVYKITNREQLKEKLALIFESDSKVMSQSFLTGIEVSCGVLERYRDGKFKKIALP
CCCCEEEEEECCHHHHHHHHHEEECCCHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEECC
ATEIVPGGEFFDFESKYKQGGSHEITPARISEQEMKRVQELAIAAHRSLGCSGYSRTDFI
HHHCCCCCCEECCHHHHHCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEE
IVNGEPHILETNTLPGMTETSLIPQQAKAAGISMEEVFSDLIEIGLKRSLY
EECCCCCEEECCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AKIAVFFGGSSTEHSISILTGCFICKTLHTMGHSVKPILLTKDGGWVVPSEYRMSIPFE
CEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEECCCCCEEECCEE
VSNSPDLFQEEFQKRYGVSRTNQIFSLDADIAFLGLHGGQGEDGTIQGFLEILGIPYTGS
ECCCCHHHHHHHHHHHCCCCCCEEEEECCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCC
GVLASAIAMDKTRANQIFLQSGQKVAPFFEIDKLEYLNSTDAVITKLETLGFPQFLKPVE
HHHHHHHHHHHHHHHHHHHHCCCEECCEEEHHHHHHCCCCHHHHHHHHHCCCHHHHHHCC
GGSSVSVYKITNREQLKEKLALIFESDSKVMSQSFLTGIEVSCGVLERYRDGKFKKIALP
CCCCEEEEEECCHHHHHHHHHEEECCCHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEECC
ATEIVPGGEFFDFESKYKQGGSHEITPARISEQEMKRVQELAIAAHRSLGCSGYSRTDFI
HHHCCCCCCEECCHHHHHCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEE
IVNGEPHILETNTLPGMTETSLIPQQAKAAGISMEEVFSDLIEIGLKRSLY
EECCCCCEEECCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA