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The map label for this gene is rfaD

Identifier: 45657488

GI number: 45657488

Start: 1978631

End: 1979611

Strand: Direct

Name: rfaD

Synonym: LIC11616

Alternate gene names: 45657488

Gene position: 1978631-1979611 (Clockwise)

Preceding gene: 45657487

Following gene: 45657492

Centisome position: 46.26

GC content: 34.15

Gene sequence:

>981_bases
TTGAATTCTATGGCAAAAAAGAAATGTATCGTTACCGGTGGTGCGGGGCTTATCGGTTCTAATCTAGTTCAAGAACTTAA
TAGACTAGGAATCGATGATATTCTAGTTGTGGATCATTTGGGAACTTCTTCCAAGTGGAAAAATTTAGTCGGAAAAAGAT
ATTCAGATTATCTGGAAAAAAAAACATTCTTAGAACATACAACTCGAAACTTTCTATTAAAAGATTATGATATTTTGTTT
CATTTGGGAGCCTGTTCTTCTACTACAGAGACGGATGCGTCTTATTTAGTGGAAAATAACTTTGAATATACAAAACTTCT
TGCCGAAGAATCTTTAAAAAACGGAGTTCGATTTGTATATGCTTCTTCCGCCGCTACCTATGGAGACGGAGCCAATGGAT
ACGATGATAAAACTCCCATTGACTTACTCAAACCTTTAAATATGTACGGATATTCAAAACATATGTTTGATTTATACGCT
CAAAGAAGAGGGTTTTTAAATCGAATTACTGGAATCAAATATTTCAATGTATTTGGATATGGAGAAGGACATAAGGGGGA
TATGAGAAGTGTAGTTCTTAAGGGGTACGAACAAATCAAAAAAGAAGGTAAAATCCGTCTTTTTAAATCGTATAAACCCG
AATATAAAGACGGAGAACAAAAAAGAGATTTTTTATACGCAAAAGATGCCGCAAAAATCACCGCCTATTTGGCATTTGGC
GATCATAACGGAATTTATAATTTAGGAAGAGGAATTGCAGAAACCTGGAACGACCTAGTATTCGCAATTTTTGATACTTT
AAAACTTTCTATCAATATAGAATATATTGAAATGCCAGAGACTCTTAAATCAAAATACCAATATTATACTTGTGCAGATA
CCACAAAACTTTTAAAAACCGGTTATTCGGAAGGTTTTACTCAATTAAAGGACGCAGTCAGTGAATATGTAACCCTCCTC
GATGAAGAAGAGGGTACATAA

Upstream 100 bases:

>100_bases
GAATGAAAACTTGGTATCCTCCACTGACAGAAGCAGATTCTAAAACGATCCGAAAAGTAGATGATAGATTTGGTAGACTG
ATAGACTCACTTTAGTAGAT

Downstream 100 bases:

>100_bases
TTAATTTTTAAAGTAATCGTTTAAAGATTAACCGTAGATTTTAACAATCGTACGAATCAGATCCTTATACTTTCCGTCTT
TTAGGTAATAGAAAACTTGG

Product: ADP-L-glycero-D-mannoheptose-6-epimerase

Products: NA

Alternate protein names: ADP-L-glycero-beta-D-manno-heptose-6-epimerase; ADP-glyceromanno-heptose 6-epimerase; ADP-hep 6-epimerase; AGME [H]

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MNSMAKKKCIVTGGAGLIGSNLVQELNRLGIDDILVVDHLGTSSKWKNLVGKRYSDYLEKKTFLEHTTRNFLLKDYDILF
HLGACSSTTETDASYLVENNFEYTKLLAEESLKNGVRFVYASSAATYGDGANGYDDKTPIDLLKPLNMYGYSKHMFDLYA
QRRGFLNRITGIKYFNVFGYGEGHKGDMRSVVLKGYEQIKKEGKIRLFKSYKPEYKDGEQKRDFLYAKDAAKITAYLAFG
DHNGIYNLGRGIAETWNDLVFAIFDTLKLSINIEYIEMPETLKSKYQYYTCADTTKLLKTGYSEGFTQLKDAVSEYVTLL
DEEEGT

Sequences:

>Translated_326_residues
MNSMAKKKCIVTGGAGLIGSNLVQELNRLGIDDILVVDHLGTSSKWKNLVGKRYSDYLEKKTFLEHTTRNFLLKDYDILF
HLGACSSTTETDASYLVENNFEYTKLLAEESLKNGVRFVYASSAATYGDGANGYDDKTPIDLLKPLNMYGYSKHMFDLYA
QRRGFLNRITGIKYFNVFGYGEGHKGDMRSVVLKGYEQIKKEGKIRLFKSYKPEYKDGEQKRDFLYAKDAAKITAYLAFG
DHNGIYNLGRGIAETWNDLVFAIFDTLKLSINIEYIEMPETLKSKYQYYTCADTTKLLKTGYSEGFTQLKDAVSEYVTLL
DEEEGT
>Mature_326_residues
MNSMAKKKCIVTGGAGLIGSNLVQELNRLGIDDILVVDHLGTSSKWKNLVGKRYSDYLEKKTFLEHTTRNFLLKDYDILF
HLGACSSTTETDASYLVENNFEYTKLLAEESLKNGVRFVYASSAATYGDGANGYDDKTPIDLLKPLNMYGYSKHMFDLYA
QRRGFLNRITGIKYFNVFGYGEGHKGDMRSVVLKGYEQIKKEGKIRLFKSYKPEYKDGEQKRDFLYAKDAAKITAYLAFG
DHNGIYNLGRGIAETWNDLVFAIFDTLKLSINIEYIEMPETLKSKYQYYTCADTTKLLKTGYSEGFTQLKDAVSEYVTLL
DEEEGT

Specific function: Catalyzes the interconversion between ADP-D-glycero- beta-D-manno-heptose and ADP-L-glycero-beta-D-manno-heptose via an epimerization at carbon 6 of the heptose [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. HldD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790049, Length=317, Percent_Identity=40.6940063091483, Blast_Score=217, Evalue=7e-58,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR011912
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =5.1.3.20 [H]

Molecular weight: Translated: 37055; Mature: 37055

Theoretical pI: Translated: 7.12; Mature: 7.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSMAKKKCIVTGGAGLIGSNLVQELNRLGIDDILVVDHLGTSSKWKNLVGKRYSDYLEK
CCCCHHCCEEEECCCHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHH
KTFLEHTTRNFLLKDYDILFHLGACSSTTETDASYLVENNFEYTKLLAEESLKNGVRFVY
HHHHHHHHHHHHHHCEEEEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHCCEEEEE
ASSAATYGDGANGYDDKTPIDLLKPLNMYGYSKHMFDLYAQRRGFLNRITGIKYFNVFGY
ECCCCCCCCCCCCCCCCCCHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEC
GEGHKGDMRSVVLKGYEQIKKEGKIRLFKSYKPEYKDGEQKRDFLYAKDAAKITAYLAFG
CCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHEEEECCCCEEEEEEEEC
DHNGIYNLGRGIAETWNDLVFAIFDTLKLSINIEYIEMPETLKSKYQYYTCADTTKLLKT
CCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEECCHHHHHCCCEEEEHHHHHHHHC
GYSEGFTQLKDAVSEYVTLLDEEEGT
CHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNSMAKKKCIVTGGAGLIGSNLVQELNRLGIDDILVVDHLGTSSKWKNLVGKRYSDYLEK
CCCCHHCCEEEECCCHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHH
KTFLEHTTRNFLLKDYDILFHLGACSSTTETDASYLVENNFEYTKLLAEESLKNGVRFVY
HHHHHHHHHHHHHHCEEEEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHCCEEEEE
ASSAATYGDGANGYDDKTPIDLLKPLNMYGYSKHMFDLYAQRRGFLNRITGIKYFNVFGY
ECCCCCCCCCCCCCCCCCCHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEC
GEGHKGDMRSVVLKGYEQIKKEGKIRLFKSYKPEYKDGEQKRDFLYAKDAAKITAYLAFG
CCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHEEEECCCCEEEEEEEEC
DHNGIYNLGRGIAETWNDLVFAIFDTLKLSINIEYIEMPETLKSKYQYYTCADTTKLLKT
CCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEECCHHHHHCCCEEEEHHHHHHHHC
GYSEGFTQLKDAVSEYVTLLDEEEGT
CHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA