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The map label for this gene is ychF

Identifier: 45657485

GI number: 45657485

Start: 1974564

End: 1975661

Strand: Direct

Name: ychF

Synonym: LIC11613

Alternate gene names: 45657485

Gene position: 1974564-1975661 (Clockwise)

Preceding gene: 45657484

Following gene: 45657486

Centisome position: 46.17

GC content: 38.8

Gene sequence:

>1098_bases
ATGAGTCTGAATTGCGGTATTGTAGGTCTTCCCAACGTAGGTAAATCCACTATTTTTAACGCCCTTACAAAAGCGGGGGC
GCAAATGGAAAATTATCCTTTTTGTACCATTGAACCCAACAAAGGAATTGTAGAAGTTCCAGATTTGCGTCTGAATCGAC
TGGCAGAAATTGCAAAACCTCAAAAGGTAGTTCCTGCCATCATTGAATTTGTAGACATCGCCGGTCTTGTTAAAGGTGCC
AGTCAAGGAGAAGGCCTTGGAAATAAATTTCTTTCCCATATCCGAGAAGTAGACGCGATTTGTCACGTTGTGCGCGCTTT
TGAAGATGAAAATGTTACTCACGTTCACGGAAAAGTGAATCCTGTGGAGGACGCTGCCATTGTAAATATGGAATTGATTT
TCGCTGATTTAGATAGTGCGGATAAACAATTTCAAAGGGTTTCAAAAAATGCTAAGAACGGAAACAAAGAAGCGCAGGAA
CATGCTTCCGTTCTTGAAAAAATCTTAACTCTTTTAAAAGCGGGAAAACCAGCAAGGCTTGCCGAATTGAAAGACGAAGA
AAAAAAAATCGCCAAGTCTTTTCAATTGATTACGTTAAAACCAGTTATGTATGTGGCGAACATTGCTGATAAGGACGCCG
CCAAAAAAGATACACCTTTACTAAATCAGATCAGACAAATGGCAAAAGAAGAAAACGCAGAGCTCGTAATTCTTTGTGGT
CGTTTTGAAGAAGAAATTTCCGGTTTAGATCGAAACGAACAACTCGAATTTCTAAAAGAAATTGGAGAAACCGAAAGTGG
TTTAGATCGTATGATCAAAACTGCATATAAACTTCTAGGTTTGATTACATTTTTTACCGCGGGTGAAATGGAAGTCAGGG
CTTGGACGACTCCCTTTAATAGTACAGGACCAAAAGCGGCTTCTGTAATTCACTCAGATTTTGAAAAAGGTTTTATCCGA
GCGGAGGTTATGAGTTACGAAGATTTAGATAGAGCAGGTACCCAAACAAAAGTAAAAGAGGAAGGAAAACTTCGAATCGA
AGGAAAAGAATATATCGTTCAAGACGGAGATGTAATTTATTTTAGAATTAATGCTTAA

Upstream 100 bases:

>100_bases
AGAAAGTTTTTCTCTCAATAGCGACGGTTCCGGTAAATCCTGGGAATTGGAATAACGATTTTACTTTTCGCTTCAACCTG
TTCCGGAAAATCTGGATCGC

Downstream 100 bases:

>100_bases
TAAAATACTAAATTATGATTTTCTGAAGATCAATTTTTTTTACTGAGTGTTCCAATCAATTCTTTTTTAAAACCAAATTA
CAGTGAAATTAAATAAGAAC

Product: GTP-dependent nucleic acid-binding protein EngD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 365; Mature: 364

Protein sequence:

>365_residues
MSLNCGIVGLPNVGKSTIFNALTKAGAQMENYPFCTIEPNKGIVEVPDLRLNRLAEIAKPQKVVPAIIEFVDIAGLVKGA
SQGEGLGNKFLSHIREVDAICHVVRAFEDENVTHVHGKVNPVEDAAIVNMELIFADLDSADKQFQRVSKNAKNGNKEAQE
HASVLEKILTLLKAGKPARLAELKDEEKKIAKSFQLITLKPVMYVANIADKDAAKKDTPLLNQIRQMAKEENAELVILCG
RFEEEISGLDRNEQLEFLKEIGETESGLDRMIKTAYKLLGLITFFTAGEMEVRAWTTPFNSTGPKAASVIHSDFEKGFIR
AEVMSYEDLDRAGTQTKVKEEGKLRIEGKEYIVQDGDVIYFRINA

Sequences:

>Translated_365_residues
MSLNCGIVGLPNVGKSTIFNALTKAGAQMENYPFCTIEPNKGIVEVPDLRLNRLAEIAKPQKVVPAIIEFVDIAGLVKGA
SQGEGLGNKFLSHIREVDAICHVVRAFEDENVTHVHGKVNPVEDAAIVNMELIFADLDSADKQFQRVSKNAKNGNKEAQE
HASVLEKILTLLKAGKPARLAELKDEEKKIAKSFQLITLKPVMYVANIADKDAAKKDTPLLNQIRQMAKEENAELVILCG
RFEEEISGLDRNEQLEFLKEIGETESGLDRMIKTAYKLLGLITFFTAGEMEVRAWTTPFNSTGPKAASVIHSDFEKGFIR
AEVMSYEDLDRAGTQTKVKEEGKLRIEGKEYIVQDGDVIYFRINA
>Mature_364_residues
SLNCGIVGLPNVGKSTIFNALTKAGAQMENYPFCTIEPNKGIVEVPDLRLNRLAEIAKPQKVVPAIIEFVDIAGLVKGAS
QGEGLGNKFLSHIREVDAICHVVRAFEDENVTHVHGKVNPVEDAAIVNMELIFADLDSADKQFQRVSKNAKNGNKEAQEH
ASVLEKILTLLKAGKPARLAELKDEEKKIAKSFQLITLKPVMYVANIADKDAAKKDTPLLNQIRQMAKEENAELVILCGR
FEEEISGLDRNEQLEFLKEIGETESGLDRMIKTAYKLLGLITFFTAGEMEVRAWTTPFNSTGPKAASVIHSDFEKGFIRA
EVMSYEDLDRAGTQTKVKEEGKLRIEGKEYIVQDGDVIYFRINA

Specific function: GTP-dependent nucleic acid-binding protein which may act as a translation factor [H]

COG id: COG0012

COG function: function code J; Predicted GTPase, probable translation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI58761500, Length=369, Percent_Identity=43.360433604336, Blast_Score=295, Evalue=4e-80,
Organism=Homo sapiens, GI58761502, Length=218, Percent_Identity=37.1559633027523, Blast_Score=139, Evalue=3e-33,
Organism=Escherichia coli, GI1787454, Length=364, Percent_Identity=53.2967032967033, Blast_Score=403, Evalue=1e-113,
Organism=Escherichia coli, GI1789574, Length=129, Percent_Identity=31.0077519379845, Blast_Score=72, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17509631, Length=367, Percent_Identity=45.5040871934605, Blast_Score=310, Evalue=5e-85,
Organism=Saccharomyces cerevisiae, GI6319499, Length=373, Percent_Identity=41.8230563002681, Blast_Score=262, Evalue=7e-71,
Organism=Saccharomyces cerevisiae, GI6321773, Length=381, Percent_Identity=35.1706036745407, Blast_Score=213, Evalue=3e-56,
Organism=Saccharomyces cerevisiae, GI6321649, Length=344, Percent_Identity=28.4883720930233, Blast_Score=96, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24640873, Length=368, Percent_Identity=43.75, Blast_Score=299, Evalue=2e-81,
Organism=Drosophila melanogaster, GI24640877, Length=368, Percent_Identity=43.75, Blast_Score=299, Evalue=2e-81,
Organism=Drosophila melanogaster, GI24640875, Length=368, Percent_Identity=43.75, Blast_Score=299, Evalue=2e-81,
Organism=Drosophila melanogaster, GI24640879, Length=326, Percent_Identity=41.4110429447853, Blast_Score=241, Evalue=6e-64,
Organism=Drosophila melanogaster, GI24585318, Length=104, Percent_Identity=35.5769230769231, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR004396
- InterPro:   IPR013029
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR012676
- InterPro:   IPR023192 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF06071 YchF-GTPase_C [H]

EC number: NA

Molecular weight: Translated: 40448; Mature: 40317

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLNCGIVGLPNVGKSTIFNALTKAGAQMENYPFCTIEPNKGIVEVPDLRLNRLAEIAKP
CCCCCCEEECCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCEEECCCHHHHHHHHHHCC
QKVVPAIIEFVDIAGLVKGASQGEGLGNKFLSHIREVDAICHVVRAFEDENVTHVHGKVN
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCC
PVEDAAIVNMELIFADLDSADKQFQRVSKNAKNGNKEAQEHASVLEKILTLLKAGKPARL
CCCCHHHEEHEEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCHHH
AELKDEEKKIAKSFQLITLKPVMYVANIADKDAAKKDTPLLNQIRQMAKEENAELVILCG
HHHHHHHHHHHHHHHEEEEHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEC
RFEEEISGLDRNEQLEFLKEIGETESGLDRMIKTAYKLLGLITFFTAGEMEVRAWTTPFN
CCHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCC
STGPKAASVIHSDFEKGFIRAEVMSYEDLDRAGTQTKVKEEGKLRIEGKEYIVQDGDVIY
CCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCCCCCCCCEEECCCEEEEECCCEEE
FRINA
EEECC
>Mature Secondary Structure 
SLNCGIVGLPNVGKSTIFNALTKAGAQMENYPFCTIEPNKGIVEVPDLRLNRLAEIAKP
CCCCCEEECCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCEEECCCHHHHHHHHHHCC
QKVVPAIIEFVDIAGLVKGASQGEGLGNKFLSHIREVDAICHVVRAFEDENVTHVHGKVN
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCC
PVEDAAIVNMELIFADLDSADKQFQRVSKNAKNGNKEAQEHASVLEKILTLLKAGKPARL
CCCCHHHEEHEEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCHHH
AELKDEEKKIAKSFQLITLKPVMYVANIADKDAAKKDTPLLNQIRQMAKEENAELVILCG
HHHHHHHHHHHHHHHEEEEHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEC
RFEEEISGLDRNEQLEFLKEIGETESGLDRMIKTAYKLLGLITFFTAGEMEVRAWTTPFN
CCHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCC
STGPKAASVIHSDFEKGFIRAEVMSYEDLDRAGTQTKVKEEGKLRIEGKEYIVQDGDVIY
CCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCCCCCCCCEEECCCEEEEECCCEEE
FRINA
EEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]