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The map label for this gene is leuA2

Identifier: 45657469

GI number: 45657469

Start: 1956275

End: 1957825

Strand: Direct

Name: leuA2

Synonym: LIC11597

Alternate gene names: 45657469

Gene position: 1956275-1957825 (Clockwise)

Preceding gene: 45657468

Following gene: 45657470

Centisome position: 45.74

GC content: 39.39

Gene sequence:

>1551_bases
ATGACAAAAGTAGAAACTCGATTGGAAATTTTAGACGTAACTTTGAGAGACGGGGAGCAGACCAGAGGGGTCAGTTTTTC
CACTTCCGAAAAACTAAATATCGCAAAATTTCTATTACAAAAACTAAATGTAGATCGGGTAGAGATTGCGTCTGCAAGAG
TTTCTAAAGGGGAATTGGAAACGGTCCAAAAAATCATGGAATGGGCTGCAACAGAACAGCTTACGGAAAGAATCGAAATC
TTAGGTTTTGTAGACGGGAATAAAACCGTAGATTGGATCAAAGATAGTGGGGCTAAGGTTTTAAATCTTTTGACTAAGGG
ATCGCTTCATCATTTAGAAAAACAATTAGGCAAAACTCCGAAAGAATTCTTTACAGACGTTTCTTTTGTAATAGAATACG
CGATCAAAAGCGGACTTAAAATAAACGTATATTTAGAAGATTGGTCCAACGGTTTCAGAAACAGTCCAGATTACGTCAAA
TCGCTCGTAGAACATCTAAGTAAAGAACATATAGAAAGAATTTTTCTTCCAGACACGTTAGGCGTTCTTTCGCCAGAAGA
GACGTTTCAAGGAGTGGATTCACTCATTCAAAAATACCCGGATATTCATTTTGAATTTCACGGACATAACGACTACGATC
TTTCCGTGGCAAATAGTCTTCAAGCGATTCGTGCCGGAGTCAAAGGTCTTCACGCTTCTATAAATGGTCTCGGAGAAAGA
GCCGGAAATACTCCGTTGGAAGCACTCGTAACCACGATTCATGATAAGTCTAACTCTAAAACGAACATAAACGAAATTGC
AATTACGGAAGCAAGCCGTCTTGTAGAAGTATTCAGCGGAAAAAGAATTTCTGCAAATAGACCGATCGTTGGAGAAGACG
TGTTTACTCAGACCGCGGGAGTACACGCAGACGGAGACAAAAAAGGAAATTTATACGCAAATCCTATTTTACCGGAAAGA
TTTGGTAGGAAAAGAAGTTACGCGTTAGGCAAACTTGCAGGTAAGGCGAGTATCTCCGAAAATGTAAAACAACTCGGAAT
GGTTTTAAGTGACGTGGTTTTACAAAAGGTTTTAGAAAGGGTGATCGAATTAGGAGATCAGAATAAACTAGTGACACCTG
AAGATCTTCCATTTATCATTGCGGACGTTTCTGGAAGAACCGGAGAAAAGGTACTTACAATCAAATCTTGTAATATTCAT
TCCGGAATTGGAATTCGTCCTCACGCACAAATTGAATTGGAATATCAGGGAAAGATTCATAAGGAAATTTCTGAAGGAGA
CGGAGGGTATGATGCGTTTATGAATGCACTTACTAAAATTACGAATCGCCTCGGTATTAGTATTCCTAAATTGATAGATT
ACGAAGTAAGGATTCCTCCTGGTGGAAAAACAGATGCACTTGTAGAAACTAGGATCACCTGGAACAAGTCCTTAGATTTA
GAAGAGGACCAGACTTTCAAAACGATGGGAGTTCATCCGGATCAAACGGTTGCAGCGGTTCATGCAACTGAAAAGATGCT
CAATCAAATTCTACAACCATGGCAAATCTAA

Upstream 100 bases:

>100_bases
TAAAGGAAGTAGATTATTCTCAGTTTGGAATTTCCAACCCGGGTTCCACGTTGGTCGAAGTATTAGGTCCCGATAAGGAC
GTTCTCAAAAGGTATCTCAG

Downstream 100 bases:

>100_bases
AACTATTATTGGTAGGGATTGGAAATCCAGGCCAAAAATATGTTCACAACCGGCATAACATCGGTTTTGTGATCTTGGAT
TCTCTCTTGGATTCTTCTTC

Product: 2-isopropylmalate synthase 2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 516; Mature: 515

Protein sequence:

>516_residues
MTKVETRLEILDVTLRDGEQTRGVSFSTSEKLNIAKFLLQKLNVDRVEIASARVSKGELETVQKIMEWAATEQLTERIEI
LGFVDGNKTVDWIKDSGAKVLNLLTKGSLHHLEKQLGKTPKEFFTDVSFVIEYAIKSGLKINVYLEDWSNGFRNSPDYVK
SLVEHLSKEHIERIFLPDTLGVLSPEETFQGVDSLIQKYPDIHFEFHGHNDYDLSVANSLQAIRAGVKGLHASINGLGER
AGNTPLEALVTTIHDKSNSKTNINEIAITEASRLVEVFSGKRISANRPIVGEDVFTQTAGVHADGDKKGNLYANPILPER
FGRKRSYALGKLAGKASISENVKQLGMVLSDVVLQKVLERVIELGDQNKLVTPEDLPFIIADVSGRTGEKVLTIKSCNIH
SGIGIRPHAQIELEYQGKIHKEISEGDGGYDAFMNALTKITNRLGISIPKLIDYEVRIPPGGKTDALVETRITWNKSLDL
EEDQTFKTMGVHPDQTVAAVHATEKMLNQILQPWQI

Sequences:

>Translated_516_residues
MTKVETRLEILDVTLRDGEQTRGVSFSTSEKLNIAKFLLQKLNVDRVEIASARVSKGELETVQKIMEWAATEQLTERIEI
LGFVDGNKTVDWIKDSGAKVLNLLTKGSLHHLEKQLGKTPKEFFTDVSFVIEYAIKSGLKINVYLEDWSNGFRNSPDYVK
SLVEHLSKEHIERIFLPDTLGVLSPEETFQGVDSLIQKYPDIHFEFHGHNDYDLSVANSLQAIRAGVKGLHASINGLGER
AGNTPLEALVTTIHDKSNSKTNINEIAITEASRLVEVFSGKRISANRPIVGEDVFTQTAGVHADGDKKGNLYANPILPER
FGRKRSYALGKLAGKASISENVKQLGMVLSDVVLQKVLERVIELGDQNKLVTPEDLPFIIADVSGRTGEKVLTIKSCNIH
SGIGIRPHAQIELEYQGKIHKEISEGDGGYDAFMNALTKITNRLGISIPKLIDYEVRIPPGGKTDALVETRITWNKSLDL
EEDQTFKTMGVHPDQTVAAVHATEKMLNQILQPWQI
>Mature_515_residues
TKVETRLEILDVTLRDGEQTRGVSFSTSEKLNIAKFLLQKLNVDRVEIASARVSKGELETVQKIMEWAATEQLTERIEIL
GFVDGNKTVDWIKDSGAKVLNLLTKGSLHHLEKQLGKTPKEFFTDVSFVIEYAIKSGLKINVYLEDWSNGFRNSPDYVKS
LVEHLSKEHIERIFLPDTLGVLSPEETFQGVDSLIQKYPDIHFEFHGHNDYDLSVANSLQAIRAGVKGLHASINGLGERA
GNTPLEALVTTIHDKSNSKTNINEIAITEASRLVEVFSGKRISANRPIVGEDVFTQTAGVHADGDKKGNLYANPILPERF
GRKRSYALGKLAGKASISENVKQLGMVLSDVVLQKVLERVIELGDQNKLVTPEDLPFIIADVSGRTGEKVLTIKSCNIHS
GIGIRPHAQIELEYQGKIHKEISEGDGGYDAFMNALTKITNRLGISIPKLIDYEVRIPPGGKTDALVETRITWNKSLDLE
EDQTFKTMGVHPDQTVAAVHATEKMLNQILQPWQI

Specific function: Catalyzes the condensation of pyruvate and acetyl- coenzyme A to form (R)-citramalate [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=518, Percent_Identity=27.2200772200772, Blast_Score=144, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6320071, Length=387, Percent_Identity=27.1317829457364, Blast_Score=109, Evalue=9e-25,
Organism=Saccharomyces cerevisiae, GI6320019, Length=375, Percent_Identity=26.9333333333333, Blast_Score=109, Evalue=1e-24,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR011830
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.1.182 [H]

Molecular weight: Translated: 57306; Mature: 57175

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKVETRLEILDVTLRDGEQTRGVSFSTSEKLNIAKFLLQKLNVDRVEIASARVSKGELE
CCCHHHEEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEHHHHHCCCHHHH
TVQKIMEWAATEQLTERIEILGFVDGNKTVDWIKDSGAKVLNLLTKGSLHHLEKQLGKTP
HHHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHCCCHHHHHHHHCCHHHHHHHHHCCCH
KEFFTDVSFVIEYAIKSGLKINVYLEDWSNGFRNSPDYVKSLVEHLSKEHIERIFLPDTL
HHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
GVLSPEETFQGVDSLIQKYPDIHFEFHGHNDYDLSVANSLQAIRAGVKGLHASINGLGER
CCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
AGNTPLEALVTTIHDKSNSKTNINEIAITEASRLVEVFSGKRISANRPIVGEDVFTQTAG
CCCCHHHHHHHHHHCCCCCCCCCHHEEHHHHHHHHHHHCCCEECCCCCCCCCHHHHHCCC
VHADGDKKGNLYANPILPERFGRKRSYALGKLAGKASISENVKQLGMVLSDVVLQKVLER
CCCCCCCCCCEEECCCCHHHHCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHH
VIELGDQNKLVTPEDLPFIIADVSGRTGEKVLTIKSCNIHSGIGIRPHAQIELEYQGKIH
HHHCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEEECCCCCCCCCCCCCEEEEEECCCHH
KEISEGDGGYDAFMNALTKITNRLGISIPKLIDYEVRIPPGGKTDALVETRITWNKSLDL
HHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCEEEEEEEECCCCCC
EEDQTFKTMGVHPDQTVAAVHATEKMLNQILQPWQI
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TKVETRLEILDVTLRDGEQTRGVSFSTSEKLNIAKFLLQKLNVDRVEIASARVSKGELE
CCHHHEEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEHHHHHCCCHHHH
TVQKIMEWAATEQLTERIEILGFVDGNKTVDWIKDSGAKVLNLLTKGSLHHLEKQLGKTP
HHHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHCCCHHHHHHHHCCHHHHHHHHHCCCH
KEFFTDVSFVIEYAIKSGLKINVYLEDWSNGFRNSPDYVKSLVEHLSKEHIERIFLPDTL
HHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
GVLSPEETFQGVDSLIQKYPDIHFEFHGHNDYDLSVANSLQAIRAGVKGLHASINGLGER
CCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
AGNTPLEALVTTIHDKSNSKTNINEIAITEASRLVEVFSGKRISANRPIVGEDVFTQTAG
CCCCHHHHHHHHHHCCCCCCCCCHHEEHHHHHHHHHHHCCCEECCCCCCCCCHHHHHCCC
VHADGDKKGNLYANPILPERFGRKRSYALGKLAGKASISENVKQLGMVLSDVVLQKVLER
CCCCCCCCCCEEECCCCHHHHCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHH
VIELGDQNKLVTPEDLPFIIADVSGRTGEKVLTIKSCNIHSGIGIRPHAQIELEYQGKIH
HHHCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEEECCCCCCCCCCCCCEEEEEECCCHH
KEISEGDGGYDAFMNALTKITNRLGISIPKLIDYEVRIPPGGKTDALVETRITWNKSLDL
HHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCEEEEEEEECCCCCC
EEDQTFKTMGVHPDQTVAAVHATEKMLNQILQPWQI
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]