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The map label for this gene is gspE

Identifier: 45657444

GI number: 45657444

Start: 1931519

End: 1933192

Strand: Direct

Name: gspE

Synonym: LIC11571

Alternate gene names: 45657444

Gene position: 1931519-1933192 (Clockwise)

Preceding gene: 45657443

Following gene: 45657445

Centisome position: 45.16

GC content: 40.26

Gene sequence:

>1674_bases
TTGAAAACTCTCGGAGATATCCTAATCGAAGAGGGGATCATATCTGAAAAAGATCTGGAAGATTCCCTAAAGGTTCAGAA
AAAAAACAACCTTCCACTCAGTCATATCATTCAGAAAAAAGGAATCGCCGGAGAAGCAGATATTCTCCGCGCCCTATCCA
AACTCTATCAACTCGAATTCAGAGAAAAGTTAGAGTTTACGGGTATGGAAGAAATATTTTTACAAATTCCTCTAAAACTC
ATTCAGAAAAGTAGAATCGTTCCGTTTTCTCTTTCTAAAAAAACAATTCGAATTGCTGTATCCGATCCTTCTGATTTACA
TCCGATGGATGACGCTCGTAACTTTCTAAAAGGTTACAACGTGGAATTTATTCTCGCTCCGGAACCGGAGATCATGAGAA
TTATTCATTCTCATTTTGATAATACATCTTCTGCCGCCAAAGAGATGTTAAATGAGATGGAGGGAAGTTTTTCCGAACTT
GCGGAAGCCTTTGAAAATGAAACCCTGGATCTAAGCGATGACGCTCCGATCATCAAAATGGTCAACGTGATCCTTTCCCA
AGCGGTCAACGAAAGGGCTTCGGATATTCACATTGAACCTTACGAAAAGTCTCTCGTGGTTCGCTATCGTGTGGATGGTA
TTTTGCATAACGTGCTTAGTCCTCCTAAATCGTATCACGCGGGTATTTCTTCCAGAATCAAGATCATGTCTAATTTGAAC
ATCGCTGAAAATAGACTTCCTCAAGACGGAAGGATCAAACTTAGATTGGCTGGTAAGGATATAGATATCCGGGTTTCCAC
CATTCCTTGTCAGTTCGGAGAACGGATCGTAATGAGGCTTTTGAATAAAACGGATCAAAAGTATTCTTTAGATACGATGG
GTTTTTATCCAGAATTAATCCAGTCTTTGCGTTCGTTAATTTACGAACCTCATGGAATCATTTTAGTAACGGGACCGACC
GGATCGGGTAAGTCTACTACATTATATTCTGCGTTAAGCGAACTCAATACAGAAGAAAGAAACATCATCACCTGCGAAGA
TCCTGTGGAATATCAGATTGAAGGAATTTCTCAAATGCAAATGCAGGAAAAAATTGGTCTTACGTTTGCAACCGGACTTC
GAGCCATCCTTCGACAAGACCCGGACGTTATCATGGTAGGTGAGATTCGAGATGAAGAAACCGCAAGAATCGCGATCCAA
GCTTCTCTGACCGGTCACCTTGTTTTTTCTACGCTTCACACAAACGATGCAGCAAGTGCCGCGACTCGTTTGATCGATAT
GGGAATCGAGCCCTATCTGATTACTTCCACAGTATTAGGATTTATGGCTCAAAGACTTGTAAGAGTTATCTGCACTCAAT
GTAAAGAAACGTATAAACCTACCACTTCGGAACTAGAGTCCATTGGCATCTCTAAAAAATTTTTAAAGAACGGTAACTTA
CATCGTGGTAAAGGTTGTTCTCATTGTATGGGAACTGGTTTTAAAGGTAGAATCGGAATTTATGAACTTTTACTTGTAAA
TTCTCCTCTCAAACAAGCAATTCTTCATGGTAAAGACGCTGGACAGTTAAACGAAATCGCTCTCGAGCATGGGTTTCGAA
CTCTTAAAGATTACGGAATTAGAAAGGTAGTTGATGGTGTAACTACAATCGACGAAGTTCTCAGGGTAACTTAA

Upstream 100 bases:

>100_bases
GATGACTGTTAAGAAAAAAATGCAGCAGGAACGTTACGAACTCGAGAGAGAAAGAATCCTCAACAAAGAAAGAGAAATCA
GAGAAAGAGGGGATTAAAAT

Downstream 100 bases:

>100_bases
GCGTCATGGCGATTTATTCTTACGTTGCATTTAATAAGAAGGGTAAAGAAGAAAAAGGAATTATAGACGCGGCTTCTCTT
CAAGCTGCAAGATCTAAATT

Product: general secretory pathway protein E

Products: NA

Alternate protein names: Type II traffic warden ATPase [H]

Number of amino acids: Translated: 557; Mature: 557

Protein sequence:

>557_residues
MKTLGDILIEEGIISEKDLEDSLKVQKKNNLPLSHIIQKKGIAGEADILRALSKLYQLEFREKLEFTGMEEIFLQIPLKL
IQKSRIVPFSLSKKTIRIAVSDPSDLHPMDDARNFLKGYNVEFILAPEPEIMRIIHSHFDNTSSAAKEMLNEMEGSFSEL
AEAFENETLDLSDDAPIIKMVNVILSQAVNERASDIHIEPYEKSLVVRYRVDGILHNVLSPPKSYHAGISSRIKIMSNLN
IAENRLPQDGRIKLRLAGKDIDIRVSTIPCQFGERIVMRLLNKTDQKYSLDTMGFYPELIQSLRSLIYEPHGIILVTGPT
GSGKSTTLYSALSELNTEERNIITCEDPVEYQIEGISQMQMQEKIGLTFATGLRAILRQDPDVIMVGEIRDEETARIAIQ
ASLTGHLVFSTLHTNDAASAATRLIDMGIEPYLITSTVLGFMAQRLVRVICTQCKETYKPTTSELESIGISKKFLKNGNL
HRGKGCSHCMGTGFKGRIGIYELLLVNSPLKQAILHGKDAGQLNEIALEHGFRTLKDYGIRKVVDGVTTIDEVLRVT

Sequences:

>Translated_557_residues
MKTLGDILIEEGIISEKDLEDSLKVQKKNNLPLSHIIQKKGIAGEADILRALSKLYQLEFREKLEFTGMEEIFLQIPLKL
IQKSRIVPFSLSKKTIRIAVSDPSDLHPMDDARNFLKGYNVEFILAPEPEIMRIIHSHFDNTSSAAKEMLNEMEGSFSEL
AEAFENETLDLSDDAPIIKMVNVILSQAVNERASDIHIEPYEKSLVVRYRVDGILHNVLSPPKSYHAGISSRIKIMSNLN
IAENRLPQDGRIKLRLAGKDIDIRVSTIPCQFGERIVMRLLNKTDQKYSLDTMGFYPELIQSLRSLIYEPHGIILVTGPT
GSGKSTTLYSALSELNTEERNIITCEDPVEYQIEGISQMQMQEKIGLTFATGLRAILRQDPDVIMVGEIRDEETARIAIQ
ASLTGHLVFSTLHTNDAASAATRLIDMGIEPYLITSTVLGFMAQRLVRVICTQCKETYKPTTSELESIGISKKFLKNGNL
HRGKGCSHCMGTGFKGRIGIYELLLVNSPLKQAILHGKDAGQLNEIALEHGFRTLKDYGIRKVVDGVTTIDEVLRVT
>Mature_557_residues
MKTLGDILIEEGIISEKDLEDSLKVQKKNNLPLSHIIQKKGIAGEADILRALSKLYQLEFREKLEFTGMEEIFLQIPLKL
IQKSRIVPFSLSKKTIRIAVSDPSDLHPMDDARNFLKGYNVEFILAPEPEIMRIIHSHFDNTSSAAKEMLNEMEGSFSEL
AEAFENETLDLSDDAPIIKMVNVILSQAVNERASDIHIEPYEKSLVVRYRVDGILHNVLSPPKSYHAGISSRIKIMSNLN
IAENRLPQDGRIKLRLAGKDIDIRVSTIPCQFGERIVMRLLNKTDQKYSLDTMGFYPELIQSLRSLIYEPHGIILVTGPT
GSGKSTTLYSALSELNTEERNIITCEDPVEYQIEGISQMQMQEKIGLTFATGLRAILRQDPDVIMVGEIRDEETARIAIQ
ASLTGHLVFSTLHTNDAASAATRLIDMGIEPYLITSTVLGFMAQRLVRVICTQCKETYKPTTSELESIGISKKFLKNGNL
HRGKGCSHCMGTGFKGRIGIYELLLVNSPLKQAILHGKDAGQLNEIALEHGFRTLKDYGIRKVVDGVTTIDEVLRVT

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of a variety of enzymes across the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=423, Percent_Identity=48.936170212766, Blast_Score=406, Evalue=1e-114,
Organism=Escherichia coli, GI1786296, Length=440, Percent_Identity=40, Blast_Score=323, Evalue=1e-89,
Organism=Escherichia coli, GI87082188, Length=169, Percent_Identity=46.7455621301775, Blast_Score=130, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 62307; Mature: 62307

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTLGDILIEEGIISEKDLEDSLKVQKKNNLPLSHIIQKKGIAGEADILRALSKLYQLEF
CCCHHHHHHHCCCCCCCCCHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHH
REKLEFTGMEEIFLQIPLKLIQKSRIVPFSLSKKTIRIAVSDPSDLHPMDDARNFLKGYN
HHHHHHCCHHHHHHHHHHHHHHCCCCCEEECCCCEEEEEECCCCCCCCCHHHHHHHHCCC
VEFILAPEPEIMRIIHSHFDNTSSAAKEMLNEMEGSFSELAEAFENETLDLSDDAPIIKM
EEEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCHHHHH
VNVILSQAVNERASDIHIEPYEKSLVVRYRVDGILHNVLSPPKSYHAGISSRIKIMSNLN
HHHHHHHHHHHHCCCCCCCCCCCCEEEEEHHHHHHHHHHCCCHHHHCCHHHHHHHHHCCC
IAENRLPQDGRIKLRLAGKDIDIRVSTIPCQFGERIVMRLLNKTDQKYSLDTMGFYPELI
HHHHCCCCCCCEEEEEECCCEEEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHH
QSLRSLIYEPHGIILVTGPTGSGKSTTLYSALSELNTEERNIITCEDPVEYQIEGISQMQ
HHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCCCEEECCCCCHHHHCCHHHHH
MQEKIGLTFATGLRAILRQDPDVIMVGEIRDEETARIAIQASLTGHLVFSTLHTNDAASA
HHHHHCCHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEEECCEEEEEEEECCCHHHHH
ATRLIDMGIEPYLITSTVLGFMAQRLVRVICTQCKETYKPTTSELESIGISKKFLKNGNL
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHCCCC
HRGKGCSHCMGTGFKGRIGIYELLLVNSPLKQAILHGKDAGQLNEIALEHGFRTLKDYGI
CCCCCCCHHHCCCCCCCHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
RKVVDGVTTIDEVLRVT
HHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKTLGDILIEEGIISEKDLEDSLKVQKKNNLPLSHIIQKKGIAGEADILRALSKLYQLEF
CCCHHHHHHHCCCCCCCCCHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHH
REKLEFTGMEEIFLQIPLKLIQKSRIVPFSLSKKTIRIAVSDPSDLHPMDDARNFLKGYN
HHHHHHCCHHHHHHHHHHHHHHCCCCCEEECCCCEEEEEECCCCCCCCCHHHHHHHHCCC
VEFILAPEPEIMRIIHSHFDNTSSAAKEMLNEMEGSFSELAEAFENETLDLSDDAPIIKM
EEEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCHHHHH
VNVILSQAVNERASDIHIEPYEKSLVVRYRVDGILHNVLSPPKSYHAGISSRIKIMSNLN
HHHHHHHHHHHHCCCCCCCCCCCCEEEEEHHHHHHHHHHCCCHHHHCCHHHHHHHHHCCC
IAENRLPQDGRIKLRLAGKDIDIRVSTIPCQFGERIVMRLLNKTDQKYSLDTMGFYPELI
HHHHCCCCCCCEEEEEECCCEEEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHH
QSLRSLIYEPHGIILVTGPTGSGKSTTLYSALSELNTEERNIITCEDPVEYQIEGISQMQ
HHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCCCEEECCCCCHHHHCCHHHHH
MQEKIGLTFATGLRAILRQDPDVIMVGEIRDEETARIAIQASLTGHLVFSTLHTNDAASA
HHHHHCCHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEEECCEEEEEEEECCCHHHHH
ATRLIDMGIEPYLITSTVLGFMAQRLVRVICTQCKETYKPTTSELESIGISKKFLKNGNL
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHCCCC
HRGKGCSHCMGTGFKGRIGIYELLLVNSPLKQAILHGKDAGQLNEIALEHGFRTLKDYGI
CCCCCCCHHHCCCCCCCHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
RKVVDGVTTIDEVLRVT
HHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1588814; 10984043 [H]