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The map label for this gene is gspD

Identifier: 45657443

GI number: 45657443

Start: 1929725

End: 1931515

Strand: Direct

Name: gspD

Synonym: LIC11570

Alternate gene names: 45657443

Gene position: 1929725-1931515 (Clockwise)

Preceding gene: 45657442

Following gene: 45657444

Centisome position: 45.12

GC content: 37.35

Gene sequence:

>1791_bases
ATGTCCGGAACAATCAGTCAACTTTCAATCTTTAGAATATTCTCCCTTCTCATACTTTTGTTTTTAGTATGGGACAAACC
AGTCTTTCCTCAAAGTAAGAAAAAAACTTCCGCAAAGACAAAATCGGTTACTCAAGAAGAACCTTCGGAAAAAACTTTCT
ATGCAAATTGGAGAGATACAGAACTAAACGACTTTCTCAAAGGAATGAGCGCCATTTTAAAGAAAAATATTCTTTTAGAT
GAAAGTTTAAAAGGTAAAAAAATTACGATCATTTCTCAAAAGGAAATTCCGATCAAAAATGGATTTATCTTTATGAAATC
TGTTTTAGAATCTCTAGGTTTCGGAGTAGTAGAAGAACCGGATCTCATTTCGATTGTTAAAATTAAAGATGCACTTGCTA
GATCTCCTATCGTTAGGGTCGGAAAAGAATTGATTCCCGAAGAGGAAGTTGGAGATTATAGAACGATCACTCAGGTCATT
CCTATTGAAAATGTAAAACCAGAAGAATTAGAACCTATTTTAAAACGTCTTACTTCTCCTAATACGGATGTGATCGTTTA
TAGAAATACAAATACGATTGTACTTTCAGGTTCCGCCGCAGACATCAATAAATTGTTGGTACTTGTAAGCGAATTTGACG
TCAAAATCGAAGAGGCGACCCCAGGATCGATTTCATCGGCAGGTGATATTCATATTTATACTTTGGAATACAGTGAAGCT
GAAAAAATCGCGGCGACTTTAGTTAAGTTAGACAATCCTGTGATTCAGTCTGAAGATCTGGGTTCTGAAAGAAAGCCTCC
ACCGCCAGGGCAACCTATGCCTAAGGTAGATAAGATCAAGGCGGTTGGCCATAAAGAATCCAATTCTGTGATTGTTACGG
CGACAAATGCGGAATGGGCAGAAATCCGTAAAATCATTAAGGTTTTGGATTCAGCTAGAAAACAGGTTTTATTAGAAGTT
TTGATCGTGGAACTTACTTCCAGTGATTTAAACGATTTCGGGATCGATTGGAGATATAAGGGGGAAGCTTTCGGTCAATT
CAATTCTGGTCTTTCTAAAGAAGCGAATATTATCAATTCGAACGGTCAGGTCAATCCGAACATAAACACTCTGAGTGGTT
TCTCTTTAGGATTTTTAAAAGCTGGTTCGGAACAAATCATAGGAATTTTAAGCGCCAATCAAGGAAATGAAAACTTTAAC
GTGTTATCTGCTCCTCAAGTGCTTACGGTAGATAATCAAGAAGCAGAAATCAGTGTGGGTCAAGACGTGCCCGTTCGAAC
TCAGAGTAGGAACGCAGGTACTGGTGGAACGAACGCTGTAACTGTGGACAATTACGAATATCGTCCTACCGGTATCAAAC
TTAAGTTTACTCCTCACGTGAACAAAAATAATAAGATCACTCTCGAACTCTTTCAGGAGATTAAAAATATCGCAGAGATT
GCTCTCGCCGGAGGAAACCCTACTTTCAATCGAAGAGAAATTAAAACTTCTATCTCAATTGAAAACACTCAATCTATCGT
GATCGGTGGATTGATTTCGAACGATAAACAAAAAAGAATCATTAAAATTCCTTTTCTAGGAGACATTCCGTATTTAGGTC
ATCTTTTTAAAAGAACTACTGAAAAAATTAAAAAGACCAACTTGATGGTTTTTATTACGCCGCATATACTCGATAGTAGA
GAGACTGCGGATAAGATGACTGTTAAGAAAAAAATGCAGCAGGAACGTTACGAACTCGAGAGAGAAAGAATCCTCAACAA
AGAAAGAGAAATCAGAGAAAGAGGGGATTAA

Upstream 100 bases:

>100_bases
GAGATCTGGGGAGCTGTAAAAACTGCCGATAAGATTACAGTAGATGTGGAAAGAGGTAGCCAGATTCTCACCTACGAATT
TATTATCAGAAACTAAAAAG

Downstream 100 bases:

>100_bases
AATTTGAAAACTCTCGGAGATATCCTAATCGAAGAGGGGATCATATCTGAAAAAGATCTGGAAGATTCCCTAAAGGTTCA
GAAAAAAAACAACCTTCCAC

Product: general secretory pathway protein D

Products: NA

Alternate protein names: Pullulanase secretion envelope pulD [H]

Number of amino acids: Translated: 596; Mature: 595

Protein sequence:

>596_residues
MSGTISQLSIFRIFSLLILLFLVWDKPVFPQSKKKTSAKTKSVTQEEPSEKTFYANWRDTELNDFLKGMSAILKKNILLD
ESLKGKKITIISQKEIPIKNGFIFMKSVLESLGFGVVEEPDLISIVKIKDALARSPIVRVGKELIPEEEVGDYRTITQVI
PIENVKPEELEPILKRLTSPNTDVIVYRNTNTIVLSGSAADINKLLVLVSEFDVKIEEATPGSISSAGDIHIYTLEYSEA
EKIAATLVKLDNPVIQSEDLGSERKPPPPGQPMPKVDKIKAVGHKESNSVIVTATNAEWAEIRKIIKVLDSARKQVLLEV
LIVELTSSDLNDFGIDWRYKGEAFGQFNSGLSKEANIINSNGQVNPNINTLSGFSLGFLKAGSEQIIGILSANQGNENFN
VLSAPQVLTVDNQEAEISVGQDVPVRTQSRNAGTGGTNAVTVDNYEYRPTGIKLKFTPHVNKNNKITLELFQEIKNIAEI
ALAGGNPTFNRREIKTSISIENTQSIVIGGLISNDKQKRIIKIPFLGDIPYLGHLFKRTTEKIKKTNLMVFITPHILDSR
ETADKMTVKKKMQQERYELERERILNKEREIRERGD

Sequences:

>Translated_596_residues
MSGTISQLSIFRIFSLLILLFLVWDKPVFPQSKKKTSAKTKSVTQEEPSEKTFYANWRDTELNDFLKGMSAILKKNILLD
ESLKGKKITIISQKEIPIKNGFIFMKSVLESLGFGVVEEPDLISIVKIKDALARSPIVRVGKELIPEEEVGDYRTITQVI
PIENVKPEELEPILKRLTSPNTDVIVYRNTNTIVLSGSAADINKLLVLVSEFDVKIEEATPGSISSAGDIHIYTLEYSEA
EKIAATLVKLDNPVIQSEDLGSERKPPPPGQPMPKVDKIKAVGHKESNSVIVTATNAEWAEIRKIIKVLDSARKQVLLEV
LIVELTSSDLNDFGIDWRYKGEAFGQFNSGLSKEANIINSNGQVNPNINTLSGFSLGFLKAGSEQIIGILSANQGNENFN
VLSAPQVLTVDNQEAEISVGQDVPVRTQSRNAGTGGTNAVTVDNYEYRPTGIKLKFTPHVNKNNKITLELFQEIKNIAEI
ALAGGNPTFNRREIKTSISIENTQSIVIGGLISNDKQKRIIKIPFLGDIPYLGHLFKRTTEKIKKTNLMVFITPHILDSR
ETADKMTVKKKMQQERYELERERILNKEREIRERGD
>Mature_595_residues
SGTISQLSIFRIFSLLILLFLVWDKPVFPQSKKKTSAKTKSVTQEEPSEKTFYANWRDTELNDFLKGMSAILKKNILLDE
SLKGKKITIISQKEIPIKNGFIFMKSVLESLGFGVVEEPDLISIVKIKDALARSPIVRVGKELIPEEEVGDYRTITQVIP
IENVKPEELEPILKRLTSPNTDVIVYRNTNTIVLSGSAADINKLLVLVSEFDVKIEEATPGSISSAGDIHIYTLEYSEAE
KIAATLVKLDNPVIQSEDLGSERKPPPPGQPMPKVDKIKAVGHKESNSVIVTATNAEWAEIRKIIKVLDSARKQVLLEVL
IVELTSSDLNDFGIDWRYKGEAFGQFNSGLSKEANIINSNGQVNPNINTLSGFSLGFLKAGSEQIIGILSANQGNENFNV
LSAPQVLTVDNQEAEISVGQDVPVRTQSRNAGTGGTNAVTVDNYEYRPTGIKLKFTPHVNKNNKITLELFQEIKNIAEIA
LAGGNPTFNRREIKTSISIENTQSIVIGGLISNDKQKRIIKIPFLGDIPYLGHLFKRTTEKIKKTNLMVFITPHILDSRE
TADKMTVKKKMQQERYELERERILNKEREIRERGD

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of pullulanase [H]

COG id: COG1450

COG function: function code NU; Type II secretory pathway, component PulD

Gene ontology:

Cell location: Cell outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP D family [H]

Homologues:

Organism=Escherichia coli, GI87082242, Length=625, Percent_Identity=25.92, Blast_Score=183, Evalue=3e-47,
Organism=Escherichia coli, GI1789793, Length=282, Percent_Identity=22.6950354609929, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001775
- InterPro:   IPR005644
- InterPro:   IPR004846
- InterPro:   IPR013356
- InterPro:   IPR004845 [H]

Pfam domain/function: PF00263 Secretin; PF03958 Secretin_N [H]

EC number: NA

Molecular weight: Translated: 66465; Mature: 66334

Theoretical pI: Translated: 8.92; Mature: 8.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGTISQLSIFRIFSLLILLFLVWDKPVFPQSKKKTSAKTKSVTQEEPSEKTFYANWRDT
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCEEEECCCCC
ELNDFLKGMSAILKKNILLDESLKGKKITIISQKEIPIKNGFIFMKSVLESLGFGVVEEP
CHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCC
DLISIVKIKDALARSPIVRVGKELIPEEEVGDYRTITQVIPIENVKPEELEPILKRLTSP
CCEEEEEHHHHHHCCHHHHHHHHHCCCHHCCCCCHHHEEECCCCCCHHHHHHHHHHHCCC
NTDVIVYRNTNTIVLSGSAADINKLLVLVSEFDVKIEEATPGSISSAGDIHIYTLEYSEA
CCCEEEEECCCEEEEECCCCCHHHHEEEEECCCEEEECCCCCCCCCCCCEEEEEEECCHH
EKIAATLVKLDNPVIQSEDLGSERKPPPPGQPMPKVDKIKAVGHKESNSVIVTATNAEWA
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCEEEEECCCCHH
EIRKIIKVLDSARKQVLLEVLIVELTSSDLNDFGIDWRYKGEAFGQFNSGLSKEANIINS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCEEECCCCCCCCCCCCCCCCEEECC
NGQVNPNINTLSGFSLGFLKAGSEQIIGILSANQGNENFNVLSAPQVLTVDNQEAEISVG
CCEECCCCCCCCCCCEEEEECCCCCEEEEEECCCCCCCCEEEECCEEEEECCCCCEEECC
QDVPVRTQSRNAGTGGTNAVTVDNYEYRPTGIKLKFTPHVNKNNKITLELFQEIKNIAEI
CCCCCEECCCCCCCCCCCEEEECCEEECCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHE
ALAGGNPTFNRREIKTSISIENTQSIVIGGLISNDKQKRIIKIPFLGDIPYLGHLFKRTT
EEECCCCCCCCCEEEEEEEECCCCEEEEEEEECCCCCCEEEEECCCCCCHHHHHHHHHHH
EKIKKTNLMVFITPHILDSRETADKMTVKKKMQQERYELERERILNKEREIRERGD
HHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCC
>Mature Secondary Structure 
SGTISQLSIFRIFSLLILLFLVWDKPVFPQSKKKTSAKTKSVTQEEPSEKTFYANWRDT
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCEEEECCCCC
ELNDFLKGMSAILKKNILLDESLKGKKITIISQKEIPIKNGFIFMKSVLESLGFGVVEEP
CHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCC
DLISIVKIKDALARSPIVRVGKELIPEEEVGDYRTITQVIPIENVKPEELEPILKRLTSP
CCEEEEEHHHHHHCCHHHHHHHHHCCCHHCCCCCHHHEEECCCCCCHHHHHHHHHHHCCC
NTDVIVYRNTNTIVLSGSAADINKLLVLVSEFDVKIEEATPGSISSAGDIHIYTLEYSEA
CCCEEEEECCCEEEEECCCCCHHHHEEEEECCCEEEECCCCCCCCCCCCEEEEEEECCHH
EKIAATLVKLDNPVIQSEDLGSERKPPPPGQPMPKVDKIKAVGHKESNSVIVTATNAEWA
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCEEEEECCCCHH
EIRKIIKVLDSARKQVLLEVLIVELTSSDLNDFGIDWRYKGEAFGQFNSGLSKEANIINS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCEEECCCCCCCCCCCCCCCCEEECC
NGQVNPNINTLSGFSLGFLKAGSEQIIGILSANQGNENFNVLSAPQVLTVDNQEAEISVG
CCEECCCCCCCCCCCEEEEECCCCCEEEEEECCCCCCCCEEEECCEEEEECCCCCEEECC
QDVPVRTQSRNAGTGGTNAVTVDNYEYRPTGIKLKFTPHVNKNNKITLELFQEIKNIAEI
CCCCCEECCCCCCCCCCCEEEECCEEECCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHE
ALAGGNPTFNRREIKTSISIENTQSIVIGGLISNDKQKRIIKIPFLGDIPYLGHLFKRTT
EEECCCCCCCCCEEEEEEEECCCCEEEEEEEECCCCCCEEEEECCCCCCHHHHHHHHHHH
EKIKKTNLMVFITPHILDSRETADKMTVKKKMQQERYELERERILNKEREIRERGD
HHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2677007 [H]