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The map label for this gene is trmD

Identifier: 45657431

GI number: 45657431

Start: 1920309

End: 1921004

Strand: Direct

Name: trmD

Synonym: LIC11558

Alternate gene names: 45657431

Gene position: 1920309-1921004 (Clockwise)

Preceding gene: 45657430

Following gene: 45657432

Centisome position: 44.9

GC content: 40.37

Gene sequence:

>696_bases
ATGAAATTTAATTTCATCACTCTTTTTCCAGACAAAATTCAATCGTATTTTTCGGAAGGTCTTCAACAAAAAGCAATCGA
GTCCGGAGTGTTTTCGGTAAATATAATCCAACTCAGAAATTTTTCCGGAAATAAACATAACCGAGTCGACGATACGATTT
ACGGAGGTGGTCCAGGAATGCTTCTCCGAGTCGAGCCGATTCACAAAGCAATTTTATCTTTAGGAGAAGAGAAGGGGATC
GTAATTTTAACTTCCCCGTCTGGAATTCCATTTAACCAGAGTATTGCTATGGATTTGAAAAAGGGCGGAAAACCTTTGAC
GTTTATTTCCGGTTATTATGAAGGTGTGGATCATCGTGTTACGGAACATCTGGTTGACATGGAAATGTCCCTTGGAAATT
ATGTATTATCTGCCGGGGATTTGGCCAGTATTTGTATCGCAGATGCAGTGTCCAGGCTTCTACCTGGATTTTTAGGAGCA
GACGAAAGTCTCCTGGATGAATCTCATAACCATCCGGATATTTTAGAATATCCACAGTTTACGAAACCTTCGGAATACAA
TGGATGGAAAGTTCCTGACGTACTACTCAGCGGCAATCACGCTTCCATTTTAGCGTGGAGAGAACAAAATAGAAAGAAGA
TCGAAAGAGGAAATTATGAATCAACTTTTAAGAGAAGTGCTGACTCCGGATGCTGA

Upstream 100 bases:

>100_bases
GAAAATGGAGAAGAAATCCTCATTCCATTTTTACAAGTTTTTGTGGGTGATCTAGATCTAGAAAAGAATACAATTGTCTT
GATACAACCGGAGATTTGGA

Downstream 100 bases:

>100_bases
AAGAACACAAAATTTTACGGTAGGAGATACCGTTAAAGTACATTATAAAATTGTTGAGTCCGGAAAAGAAAGGGTTCAGA
TTTACGAAGGAGTTGTAATT

Product: tRNA (guanine-N(1)-)-methyltransferase/unknown domain fusion protein

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MKFNFITLFPDKIQSYFSEGLQQKAIESGVFSVNIIQLRNFSGNKHNRVDDTIYGGGPGMLLRVEPIHKAILSLGEEKGI
VILTSPSGIPFNQSIAMDLKKGGKPLTFISGYYEGVDHRVTEHLVDMEMSLGNYVLSAGDLASICIADAVSRLLPGFLGA
DESLLDESHNHPDILEYPQFTKPSEYNGWKVPDVLLSGNHASILAWREQNRKKIERGNYESTFKRSADSGC

Sequences:

>Translated_231_residues
MKFNFITLFPDKIQSYFSEGLQQKAIESGVFSVNIIQLRNFSGNKHNRVDDTIYGGGPGMLLRVEPIHKAILSLGEEKGI
VILTSPSGIPFNQSIAMDLKKGGKPLTFISGYYEGVDHRVTEHLVDMEMSLGNYVLSAGDLASICIADAVSRLLPGFLGA
DESLLDESHNHPDILEYPQFTKPSEYNGWKVPDVLLSGNHASILAWREQNRKKIERGNYESTFKRSADSGC
>Mature_231_residues
MKFNFITLFPDKIQSYFSEGLQQKAIESGVFSVNIIQLRNFSGNKHNRVDDTIYGGGPGMLLRVEPIHKAILSLGEEKGI
VILTSPSGIPFNQSIAMDLKKGGKPLTFISGYYEGVDHRVTEHLVDMEMSLGNYVLSAGDLASICIADAVSRLLPGFLGA
DESLLDESHNHPDILEYPQFTKPSEYNGWKVPDVLLSGNHASILAWREQNRKKIERGNYESTFKRSADSGC

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=210, Percent_Identity=38.5714285714286, Blast_Score=148, Evalue=4e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_LEPIC (Q72S28)

Other databases:

- EMBL:   AE016823
- RefSeq:   YP_001517.1
- ProteinModelPortal:   Q72S28
- SMR:   Q72S28
- GeneID:   2770199
- GenomeReviews:   AE016823_GR
- KEGG:   lic:LIC11558
- HOGENOM:   HBG285805
- OMA:   VCGRFEG
- ProtClustDB:   PRK14599
- BioCyc:   LINT267671:LIC_11558-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 25594; Mature: 25594

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: NA

Important sites: BINDING 111-111

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFNFITLFPDKIQSYFSEGLQQKAIESGVFSVNIIQLRNFSGNKHNRVDDTIYGGGPGM
CCCEEEEECHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCCEECCCCCE
LLRVEPIHKAILSLGEEKGIVILTSPSGIPFNQSIAMDLKKGGKPLTFISGYYEGVDHRV
EEEECHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHCCCCCEEEEHHHHHCHHHHH
TEHLVDMEMSLGNYVLSAGDLASICIADAVSRLLPGFLGADESLLDESHNHPDILEYPQF
HHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHHCCHHCCCCHHHHHCCCCCCCCCCCCCC
TKPSEYNGWKVPDVLLSGNHASILAWREQNRKKIERGNYESTFKRSADSGC
CCCCCCCCCCCCEEEECCCCEEEEEEHHHHHHHHHCCCCHHHHHHCCCCCC
>Mature Secondary Structure
MKFNFITLFPDKIQSYFSEGLQQKAIESGVFSVNIIQLRNFSGNKHNRVDDTIYGGGPGM
CCCEEEEECHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCCEECCCCCE
LLRVEPIHKAILSLGEEKGIVILTSPSGIPFNQSIAMDLKKGGKPLTFISGYYEGVDHRV
EEEECHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHCCCCCEEEEHHHHHCHHHHH
TEHLVDMEMSLGNYVLSAGDLASICIADAVSRLLPGFLGADESLLDESHNHPDILEYPQF
HHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHHCCHHCCCCHHHHHCCCCCCCCCCCCCC
TKPSEYNGWKVPDVLLSGNHASILAWREQNRKKIERGNYESTFKRSADSGC
CCCCCCCCCCCCEEEECCCCEEEEEEHHHHHHHHHCCCCHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA