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The map label for this gene is tesA

Identifier: 45657291

GI number: 45657291

Start: 1741195

End: 1741827

Strand: Direct

Name: tesA

Synonym: LIC11414

Alternate gene names: 45657291

Gene position: 1741195-1741827 (Clockwise)

Preceding gene: 45657287

Following gene: 45657292

Centisome position: 40.71

GC content: 33.97

Gene sequence:

>633_bases
ATGTTTTGTTTCAAGATAATGATTTTTATTTTCAGTTGTTTCTTTTTTACTTCCATTCATTCAGATTCTAAAACGGTTCG
AATTCTATTTTTCGGAGATAGTCTCACTGCAGGTTTTGGTCTTAATTCTCCCGAGGAGGCATTTCCTGCACTTTTAGAAA
AAGAACTTTCTAAAAAAGGAATTGTTTCTCAAACGATCAACGCGGGTGTAAGCGGGGATACAAGTTCTGGAGGACTTGCT
CGTTTGAACTGGGCGATGTCATCCGATTTTGATCTGTTTGTTTTGGAGTTAGGGGCCAACGATTCCATGAGAGGAATTTC
TCCCGATCAAACCGAAAAAAATTTAAAAGAGATCATCTCTAAAGTGAGAAAGAAAAATTCGAAAGCAAAGATTCTTCTTT
TGGGTATGAAGACATTTCCAAATTTAGGAAAAGAATATAGAAAAAAATTCGAAGCCGTGTTTCCTAAAATTGCAAAAGAA
GAAAATTTAATTTTAGTTCCTTTCTTTTTAGATGGGGTTGCAGGAATCAAAAATTTAAATCAGAAAGATGGAATTCATCC
GACTGCAGAAGGACATCGTATTCTTGCTAAAAATCTAATTCCATTTTTTCAAAAAATTTTGAAGAGTAAATGA

Upstream 100 bases:

>100_bases
CAAAAGAAGTAAGGACACGAAAAGAAAACGTCGGATCATAGTTTGACAGAATAAGAAAACCGGAAATTCTGTAAAAACAA
ATTCCGGGAGTAGAGGTGTT

Downstream 100 bases:

>100_bases
TTCTCCAAAAAGTGTGTTTACACCTTACTTTTCCTAGAAATGATGGATCTACCTAAATGATCGCGGGAATAGCTCAGTGG
TAGAGCACCTCCTTGCCAAG

Product: acyl-CoA thioesterase

Products: NA

Alternate protein names: Lecithinase B; Lysophospholipase L1; Protease I [H]

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MFCFKIMIFIFSCFFFTSIHSDSKTVRILFFGDSLTAGFGLNSPEEAFPALLEKELSKKGIVSQTINAGVSGDTSSGGLA
RLNWAMSSDFDLFVLELGANDSMRGISPDQTEKNLKEIISKVRKKNSKAKILLLGMKTFPNLGKEYRKKFEAVFPKIAKE
ENLILVPFFLDGVAGIKNLNQKDGIHPTAEGHRILAKNLIPFFQKILKSK

Sequences:

>Translated_210_residues
MFCFKIMIFIFSCFFFTSIHSDSKTVRILFFGDSLTAGFGLNSPEEAFPALLEKELSKKGIVSQTINAGVSGDTSSGGLA
RLNWAMSSDFDLFVLELGANDSMRGISPDQTEKNLKEIISKVRKKNSKAKILLLGMKTFPNLGKEYRKKFEAVFPKIAKE
ENLILVPFFLDGVAGIKNLNQKDGIHPTAEGHRILAKNLIPFFQKILKSK
>Mature_210_residues
MFCFKIMIFIFSCFFFTSIHSDSKTVRILFFGDSLTAGFGLNSPEEAFPALLEKELSKKGIVSQTINAGVSGDTSSGGLA
RLNWAMSSDFDLFVLELGANDSMRGISPDQTEKNLKEIISKVRKKNSKAKILLLGMKTFPNLGKEYRKKFEAVFPKIAKE
ENLILVPFFLDGVAGIKNLNQKDGIHPTAEGHRILAKNLIPFFQKILKSK

Specific function: Hydrolyzes only long chain acyl thioesters (C12-C18). Specificity similar to chymotrypsin [H]

COG id: COG2755

COG function: function code E; Lysophospholipase L1 and related esterases

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDSL' lipolytic enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1786702, Length=197, Percent_Identity=36.5482233502538, Blast_Score=123, Evalue=8e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013830
- InterPro:   IPR013831
- InterPro:   IPR001087
- InterPro:   IPR008265 [H]

Pfam domain/function: PF00657 Lipase_GDSL [H]

EC number: =3.1.1.5 [H]

Molecular weight: Translated: 23374; Mature: 23374

Theoretical pI: Translated: 10.27; Mature: 10.27

Prosite motif: PS01098 LIPASE_GDSL_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFCFKIMIFIFSCFFFTSIHSDSKTVRILFFGDSLTAGFGLNSPEEAFPALLEKELSKKG
CHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCC
IVSQTINAGVSGDTSSGGLARLNWAMSSDFDLFVLELGANDSMRGISPDQTEKNLKEIIS
CHHHHHHCCCCCCCCCCCEEEEEECCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHH
KVRKKNSKAKILLLGMKTFPNLGKEYRKKFEAVFPKIAKEENLILVPFFLDGVAGIKNLN
HHHHCCCCCEEEEEECHHCHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHCCC
QKDGIHPTAEGHRILAKNLIPFFQKILKSK
HHCCCCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFCFKIMIFIFSCFFFTSIHSDSKTVRILFFGDSLTAGFGLNSPEEAFPALLEKELSKKG
CHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCC
IVSQTINAGVSGDTSSGGLARLNWAMSSDFDLFVLELGANDSMRGISPDQTEKNLKEIIS
CHHHHHHCCCCCCCCCCCEEEEEECCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHH
KVRKKNSKAKILLLGMKTFPNLGKEYRKKFEAVFPKIAKEENLILVPFFLDGVAGIKNLN
HHHHCCCCCEEEEEECHHCHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHCCC
QKDGIHPTAEGHRILAKNLIPFFQKILKSK
HHCCCCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]