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The map label for this gene is trpA

Identifier: 45656751

GI number: 45656751

Start: 1036743

End: 1037537

Strand: Direct

Name: trpA

Synonym: LIC10860

Alternate gene names: 45656751

Gene position: 1036743-1037537 (Clockwise)

Preceding gene: 45656750

Following gene: 45656753

Centisome position: 24.24

GC content: 41.01

Gene sequence:

>795_bases
TTGAACTCCATTTCTTCCGTTTTTTCTTCGGAGCAAAGTGTTTTTATTCCTTATATTTCTTTGGGTGATCCGGACTATGA
TTCCTGCGTTATTTGGGCGGATGCTTTGATTCGAGGTGGTGCCGGCATTTTAGAATTGGGAATTCCTTTTACTGATCCGG
TTGCCGATGGTCCAGTGATTCAAAAAGCTTTTAAACGTTCTTTGGCTCATCCTTTTTCCATGGATAAAATTTTAGAAGTT
ACTTCGGAAATTCATAAACTTCATCCTCAGATTCCTCTCGTATATCTTACTTATTTTAATCCTTTGTTCTCTATGGGTTT
GGAATCTTTTACGGAAAGAGCTAAAAATTCTGGTATCCAAGGTTTGATCATTCCGGACCTTCCGTTTGATACCCCGGAGG
CGGAAGAATTTTTTTCTCAACTCGAAAGAAAGAGAATCGATTTTATCCATCTTGTGACTCCTGCTACTACGGAAGACAGA
ATTCGTTCGATGAAATCTCTTGCGTCCGGTTTTATCTATTACGTGACTTCTTACGGCGTTACCGGGGAAAGAGGTTCGAT
TGCGTCTGGTTTGGAAGATAGAATTCGAATGGTGCGTAAGATTGTCGGTCTTCCAGTTTGTGCTGGTTTTGGGATTTCCA
CTTCAGATCAATCTAAGGTTATTTCGACTTATGCAGATGGAGTGATCATCGGTTCTGCCGTGCAAAGAATCATTGAAGAA
AACGGATCTGATCGTAATAACTGTGCGGATAAACTTCTGGCCTACGCTTCTGAGATTCGTGCTTCGATGAGATAG

Upstream 100 bases:

>100_bases
CAAGAACTATGAGCAAAAAAGAGAATATTCTGATTTGTCTTTCCGGTAGAGGTGACAAGGACGTGGCAGAGGTTTCTAGA
CTTAGAAAAGGAGAATTTAT

Downstream 100 bases:

>100_bases
ATGACTTTTGGCTGTGTCGTTAGTTGAGGTGGATTTAGCTTGGATGTGCCCTTACGAGTTGAATTTGGCTTTGTAGACGC
TTCCGTGGAAACAGTAATTT

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MNSISSVFSSEQSVFIPYISLGDPDYDSCVIWADALIRGGAGILELGIPFTDPVADGPVIQKAFKRSLAHPFSMDKILEV
TSEIHKLHPQIPLVYLTYFNPLFSMGLESFTERAKNSGIQGLIIPDLPFDTPEAEEFFSQLERKRIDFIHLVTPATTEDR
IRSMKSLASGFIYYVTSYGVTGERGSIASGLEDRIRMVRKIVGLPVCAGFGISTSDQSKVISTYADGVIIGSAVQRIIEE
NGSDRNNCADKLLAYASEIRASMR

Sequences:

>Translated_264_residues
MNSISSVFSSEQSVFIPYISLGDPDYDSCVIWADALIRGGAGILELGIPFTDPVADGPVIQKAFKRSLAHPFSMDKILEV
TSEIHKLHPQIPLVYLTYFNPLFSMGLESFTERAKNSGIQGLIIPDLPFDTPEAEEFFSQLERKRIDFIHLVTPATTEDR
IRSMKSLASGFIYYVTSYGVTGERGSIASGLEDRIRMVRKIVGLPVCAGFGISTSDQSKVISTYADGVIIGSAVQRIIEE
NGSDRNNCADKLLAYASEIRASMR
>Mature_264_residues
MNSISSVFSSEQSVFIPYISLGDPDYDSCVIWADALIRGGAGILELGIPFTDPVADGPVIQKAFKRSLAHPFSMDKILEV
TSEIHKLHPQIPLVYLTYFNPLFSMGLESFTERAKNSGIQGLIIPDLPFDTPEAEEFFSQLERKRIDFIHLVTPATTEDR
IRSMKSLASGFIYYVTSYGVTGERGSIASGLEDRIRMVRKIVGLPVCAGFGISTSDQSKVISTYADGVIIGSAVQRIIEE
NGSDRNNCADKLLAYASEIRASMR

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=269, Percent_Identity=30.4832713754647, Blast_Score=140, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6321412, Length=234, Percent_Identity=32.0512820512821, Blast_Score=141, Evalue=1e-34,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_LEPIC (Q72U04)

Other databases:

- EMBL:   AE016823
- RefSeq:   YP_000837.1
- ProteinModelPortal:   Q72U04
- SMR:   Q72U04
- GeneID:   2772025
- GenomeReviews:   AE016823_GR
- KEGG:   lic:LIC10860
- HOGENOM:   HBG391690
- OMA:   NPIYIYG
- ProtClustDB:   PRK13111
- BioCyc:   LINT267671:LIC_10860-MONOMER
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 29028; Mature: 29028

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: ACT_SITE 45-45 ACT_SITE 56-56

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSISSVFSSEQSVFIPYISLGDPDYDSCVIWADALIRGGAGILELGIPFTDPVADGPVI
CCHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHH
QKAFKRSLAHPFSMDKILEVTSEIHKLHPQIPLVYLTYFNPLFSMGLESFTERAKNSGIQ
HHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCC
GLIIPDLPFDTPEAEEFFSQLERKRIDFIHLVTPATTEDRIRSMKSLASGFIYYVTSYGV
EEEECCCCCCCCCHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCC
TGERGSIASGLEDRIRMVRKIVGLPVCAGFGISTSDQSKVISTYADGVIIGSAVQRIIEE
CCCCCCHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHH
NGSDRNNCADKLLAYASEIRASMR
CCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNSISSVFSSEQSVFIPYISLGDPDYDSCVIWADALIRGGAGILELGIPFTDPVADGPVI
CCHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHH
QKAFKRSLAHPFSMDKILEVTSEIHKLHPQIPLVYLTYFNPLFSMGLESFTERAKNSGIQ
HHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCC
GLIIPDLPFDTPEAEEFFSQLERKRIDFIHLVTPATTEDRIRSMKSLASGFIYYVTSYGV
EEEECCCCCCCCCHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCC
TGERGSIASGLEDRIRMVRKIVGLPVCAGFGISTSDQSKVISTYADGVIIGSAVQRIIEE
CCCCCCHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHH
NGSDRNNCADKLLAYASEIRASMR
CCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA