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The map label for this gene is grpE

Identifier: 45656423

GI number: 45656423

Start: 633834

End: 634472

Strand: Reverse

Name: grpE

Synonym: LIC10525

Alternate gene names: 45656423

Gene position: 634472-633834 (Counterclockwise)

Preceding gene: 45656424

Following gene: 45656422

Centisome position: 14.83

GC content: 38.5

Gene sequence:

>639_bases
ATGGCCGAAACGTCAAACAATAAAACTTCGGAAGAAGCAAAGGCCAATGAGAAAAAATCTCAATCAGAAACATTAGAGGA
AAGTAAATTAGAAAATATGAATTCTGAAGAATCGACTGAAACAACACAAACTGAGTCAATGGAAACGGCGGAGACGGAAA
CTTCACTACAAACCGAATTGGAATCCGCAAAAAAAGAGATAGAGTCTTTGAAAGATTCTTGGGCAAGAGAAAGGGCTGAA
TTTCAAAACTTCAAACGTCGCTCTGCTCAGGAATTCGTTTCAATCCGCAAAGAAGCGGTCAAATCTCTTGTAAGCGGTTT
TCTAAATCCAATTGACAATTTAGAAAGAGTGGGAGCAACTCAAACCAACTCGGAAGAACTAAAACCATTTGTGGATGGAG
TTACAATGATTTTGAAGGAATTTTATTCCGTATTAGAAAAATCGAATGTAATTCGTTTTGATCCAAAAGGAGAACCGTTC
GACCCTATGTCTATGGAAGCCCTTTCTTCGGAAGAAGGAGACCAATACTCGGAAGAGACTGTAATAGACGTCTATCAACC
GGGTTATTACTATAAGGAAAACGAAGATAAGTTTACACTTCGACCTGCAAGGGTTCGAATTGGTAAACCGAAATCATAA

Upstream 100 bases:

>100_bases
CAGAGAATGGATTACAATAGAGCCTTACCGCTCGTCGACTTCACGTCGAAACTTGTCTCCGAAATGGTGACTCGCATTAG
TAAATAAGGGAGAATTCAAT

Downstream 100 bases:

>100_bases
TTAGAATCTCAGGGAAGTATAAAGGAGAAAACAAATGTCCAAAGAAAAAATTATAGGTATCGATTTAGGAACTACTAACT
CGGTCGTTTCCGTGATGGAA

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 212; Mature: 211

Protein sequence:

>212_residues
MAETSNNKTSEEAKANEKKSQSETLEESKLENMNSEESTETTQTESMETAETETSLQTELESAKKEIESLKDSWARERAE
FQNFKRRSAQEFVSIRKEAVKSLVSGFLNPIDNLERVGATQTNSEELKPFVDGVTMILKEFYSVLEKSNVIRFDPKGEPF
DPMSMEALSSEEGDQYSEETVIDVYQPGYYYKENEDKFTLRPARVRIGKPKS

Sequences:

>Translated_212_residues
MAETSNNKTSEEAKANEKKSQSETLEESKLENMNSEESTETTQTESMETAETETSLQTELESAKKEIESLKDSWARERAE
FQNFKRRSAQEFVSIRKEAVKSLVSGFLNPIDNLERVGATQTNSEELKPFVDGVTMILKEFYSVLEKSNVIRFDPKGEPF
DPMSMEALSSEEGDQYSEETVIDVYQPGYYYKENEDKFTLRPARVRIGKPKS
>Mature_211_residues
AETSNNKTSEEAKANEKKSQSETLEESKLENMNSEESTETTQTESMETAETETSLQTELESAKKEIESLKDSWARERAEF
QNFKRRSAQEFVSIRKEAVKSLVSGFLNPIDNLERVGATQTNSEELKPFVDGVTMILKEFYSVLEKSNVIRFDPKGEPFD
PMSMEALSSEEGDQYSEETVIDVYQPGYYYKENEDKFTLRPARVRIGKPKS

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Saccharomyces cerevisiae, GI6324806, Length=180, Percent_Identity=28.8888888888889, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_LEPIC (P61444)

Other databases:

- EMBL:   AF007813
- EMBL:   AE016823
- RefSeq:   YP_000509.1
- ProteinModelPortal:   P61444
- SMR:   P61444
- GeneID:   2771883
- GenomeReviews:   AE016823_GR
- KEGG:   lic:LIC10525
- NMPDR:   fig|267671.1.peg.509
- HOGENOM:   HBG732630
- OMA:   EVEPNSV
- ProtClustDB:   PRK14146
- BioCyc:   LINT-130-01:LINT-130-01-000509-MONOMER
- BioCyc:   LINT267671:LIC_10525-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 24232; Mature: 24101

Theoretical pI: Translated: 4.36; Mature: 4.36

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAETSNNKTSEEAKANEKKSQSETLEESKLENMNSEESTETTQTESMETAETETSLQTEL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ESAKKEIESLKDSWARERAEFQNFKRRSAQEFVSIRKEAVKSLVSGFLNPIDNLERVGAT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
QTNSEELKPFVDGVTMILKEFYSVLEKSNVIRFDPKGEPFDPMSMEALSSEEGDQYSEET
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCHHHHHHCCCCCCCHHHHH
VIDVYQPGYYYKENEDKFTLRPARVRIGKPKS
EEEEECCCCEEECCCCCEEECHHHHCCCCCCC
>Mature Secondary Structure 
AETSNNKTSEEAKANEKKSQSETLEESKLENMNSEESTETTQTESMETAETETSLQTEL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ESAKKEIESLKDSWARERAEFQNFKRRSAQEFVSIRKEAVKSLVSGFLNPIDNLERVGAT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
QTNSEELKPFVDGVTMILKEFYSVLEKSNVIRFDPKGEPFDPMSMEALSSEEGDQYSEET
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCHHHHHHCCCCCCCHHHHH
VIDVYQPGYYYKENEDKFTLRPARVRIGKPKS
EEEEECCCCEEECCCCCEEECHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9714717