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The map label for this gene is prfB

Identifier: 45656368

GI number: 45656368

Start: 541840

End: 542943

Strand: Reverse

Name: prfB

Synonym: LIC10470

Alternate gene names: 45656368

Gene position: 542943-541840 (Counterclockwise)

Preceding gene: 45656369

Following gene: 45656367

Centisome position: 12.69

GC content: 39.86

Gene sequence:

>1104_bases
ATGGAAGTTAAATCGGCAAAAGAACTCAAAAGAATCTCTAAAGAATTACAAGAGAATTTTTTAAACCGTTGGAAATTGTT
GAATTTGGAACAAGATAAAGATCGTCTCAAGTCGCTCACAGAAAAAGCGGAAGATCCAAATCTTTGGAACAATCCAGAAG
AAGCCAGACTTGTAAGTCAGAAAAAAAACGAACTCGAAAAAAAACTCAATCCTTGGTTTACTATTCAGCAGGATATATTA
GATTTTCCTGATTTAGTGGATCTCACCTTGGACGAAAAAGGCGAAAACGGGGTTGGAGAACTTTCTTCCGAATACAATAG
ACTTCAGGAAAAGTTCGAGGAATTAGAACTTTTAGGCGCTTTAAAAAATCCGGAAGATTTAAAACCTGCCTTTTTAAACA
TTCATCCAGGAGCCGGCGGAACCGAAAGTCAAGACTGGGCGGAAATGCTCCTCAGAATGTACACACGTTATTTCGAAAAA
AAAGGGTATCAGTATTCTCTAATAGACGTTCAAGCAGGAGACGGCGCCGGTATTAAAAATGCCACCTTACACGTAATAGG
TGATTTTGCATTCGGATTTCTTAAAGGCGAAAACGGAGTTCACAGACTCGTTCGGATTTCTCCCTTTGACGCGAATAAAA
GAAGACATACTTCTTTCGTTTCAGTTCACGTCAGCCCCGAAATAGACGACGATATAGACATCAAAATCGAAGAGAAAGAT
ATTCGTGTAGACGTTTATCGTTCTTCGGGCGCGGGCGGTCAGCACGTAAATACTACCGACTCCGCGGTTCGAATCACCCA
CATGCCGAGTGGTATCGTAGTCGCTTGTCAAAATGAAAGGTCTCAGATTAAAAACAGAGATACTGCATTCAAAATGTTGA
AAGCTAGACTTTACGAGTTGGAACAAGAAAAAGCAAAGGAAGAGTTGGAAAAAAAATCCGGAGAAAAAAAAGACATCACT
TGGGGTTCTCAGATTCGTAGTTATGTATTTCATCCTTATAATCTTGTAAAAGATCATAGAACCGATCACGAAACCGGTAA
CGTTGCAGCCGTGATGGACGGTGACATAGAACCATTCATTCTTGCTTATTTGAAAACGCTTTAA

Upstream 100 bases:

>100_bases
TCATTAAAATGGGGAAAAAATTATTATGAAATTTAAATAGAATCAAGTTGGAATCGTTATTATTCTATCGAATTTCAATC
AAATCTTAGTGTAGATAGTT

Downstream 100 bases:

>100_bases
GTTTTTTGAATGTCTTTTGGGACGGCTCCTAAGTTATGTTTTGGGAGTAGCTCGTTGAGTTTGCACAGGATCGGGCTAAG
GCCTTCGGTATAACTTTTTC

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MEVKSAKELKRISKELQENFLNRWKLLNLEQDKDRLKSLTEKAEDPNLWNNPEEARLVSQKKNELEKKLNPWFTIQQDIL
DFPDLVDLTLDEKGENGVGELSSEYNRLQEKFEELELLGALKNPEDLKPAFLNIHPGAGGTESQDWAEMLLRMYTRYFEK
KGYQYSLIDVQAGDGAGIKNATLHVIGDFAFGFLKGENGVHRLVRISPFDANKRRHTSFVSVHVSPEIDDDIDIKIEEKD
IRVDVYRSSGAGGQHVNTTDSAVRITHMPSGIVVACQNERSQIKNRDTAFKMLKARLYELEQEKAKEELEKKSGEKKDIT
WGSQIRSYVFHPYNLVKDHRTDHETGNVAAVMDGDIEPFILAYLKTL

Sequences:

>Translated_367_residues
MEVKSAKELKRISKELQENFLNRWKLLNLEQDKDRLKSLTEKAEDPNLWNNPEEARLVSQKKNELEKKLNPWFTIQQDIL
DFPDLVDLTLDEKGENGVGELSSEYNRLQEKFEELELLGALKNPEDLKPAFLNIHPGAGGTESQDWAEMLLRMYTRYFEK
KGYQYSLIDVQAGDGAGIKNATLHVIGDFAFGFLKGENGVHRLVRISPFDANKRRHTSFVSVHVSPEIDDDIDIKIEEKD
IRVDVYRSSGAGGQHVNTTDSAVRITHMPSGIVVACQNERSQIKNRDTAFKMLKARLYELEQEKAKEELEKKSGEKKDIT
WGSQIRSYVFHPYNLVKDHRTDHETGNVAAVMDGDIEPFILAYLKTL
>Mature_367_residues
MEVKSAKELKRISKELQENFLNRWKLLNLEQDKDRLKSLTEKAEDPNLWNNPEEARLVSQKKNELEKKLNPWFTIQQDIL
DFPDLVDLTLDEKGENGVGELSSEYNRLQEKFEELELLGALKNPEDLKPAFLNIHPGAGGTESQDWAEMLLRMYTRYFEK
KGYQYSLIDVQAGDGAGIKNATLHVIGDFAFGFLKGENGVHRLVRISPFDANKRRHTSFVSVHVSPEIDDDIDIKIEEKD
IRVDVYRSSGAGGQHVNTTDSAVRITHMPSGIVVACQNERSQIKNRDTAFKMLKARLYELEQEKAKEELEKKSGEKKDIT
WGSQIRSYVFHPYNLVKDHRTDHETGNVAAVMDGDIEPFILAYLKTL

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI34577120, Length=311, Percent_Identity=34.7266881028939, Blast_Score=162, Evalue=4e-40,
Organism=Homo sapiens, GI166795303, Length=230, Percent_Identity=38.2608695652174, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI166795305, Length=140, Percent_Identity=37.1428571428571, Blast_Score=106, Evalue=3e-23,
Organism=Escherichia coli, GI2367172, Length=340, Percent_Identity=47.3529411764706, Blast_Score=326, Evalue=2e-90,
Organism=Escherichia coli, GI1787462, Length=315, Percent_Identity=36.1904761904762, Blast_Score=179, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17542784, Length=237, Percent_Identity=31.6455696202532, Blast_Score=108, Evalue=6e-24,
Organism=Saccharomyces cerevisiae, GI6321295, Length=303, Percent_Identity=36.6336633663366, Blast_Score=159, Evalue=7e-40,
Organism=Drosophila melanogaster, GI19921226, Length=229, Percent_Identity=41.0480349344978, Blast_Score=173, Evalue=2e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 42063; Mature: 42063

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVKSAKELKRISKELQENFLNRWKLLNLEQDKDRLKSLTEKAEDPNLWNNPEEARLVSQ
CCCCCHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
KKNELEKKLNPWFTIQQDILDFPDLVDLTLDEKGENGVGELSSEYNRLQEKFEELELLGA
HHHHHHHHCCCCEEEHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LKNPEDLKPAFLNIHPGAGGTESQDWAEMLLRMYTRYFEKKGYQYSLIDVQAGDGAGIKN
CCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCC
ATLHVIGDFAFGFLKGENGVHRLVRISPFDANKRRHTSFVSVHVSPEIDDDIDIKIEEKD
CEEEEEEHHHHHEEECCCCCEEEEEECCCCCCCCCCCEEEEEEECCCCCCCCEEEEECCC
IRVDVYRSSGAGGQHVNTTDSAVRITHMPSGIVVACQNERSQIKNRDTAFKMLKARLYEL
EEEEEEECCCCCCCCCCCCCCEEEEEECCCCEEEEECCCHHHHHCHHHHHHHHHHHHHHH
EQEKAKEELEKKSGEKKDITWGSQIRSYVFHPYNLVKDHRTDHETGNVAAVMDGDIEPFI
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHHCCCCCCCCCCEEEEECCCCCHHH
LAYLKTL
HHHHHHC
>Mature Secondary Structure
MEVKSAKELKRISKELQENFLNRWKLLNLEQDKDRLKSLTEKAEDPNLWNNPEEARLVSQ
CCCCCHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
KKNELEKKLNPWFTIQQDILDFPDLVDLTLDEKGENGVGELSSEYNRLQEKFEELELLGA
HHHHHHHHCCCCEEEHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LKNPEDLKPAFLNIHPGAGGTESQDWAEMLLRMYTRYFEKKGYQYSLIDVQAGDGAGIKN
CCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCC
ATLHVIGDFAFGFLKGENGVHRLVRISPFDANKRRHTSFVSVHVSPEIDDDIDIKIEEKD
CEEEEEEHHHHHEEECCCCCEEEEEECCCCCCCCCCCEEEEEEECCCCCCCCEEEEECCC
IRVDVYRSSGAGGQHVNTTDSAVRITHMPSGIVVACQNERSQIKNRDTAFKMLKARLYEL
EEEEEEECCCCCCCCCCCCCCEEEEEECCCCEEEEECCCHHHHHCHHHHHHHHHHHHHHH
EQEKAKEELEKKSGEKKDITWGSQIRSYVFHPYNLVKDHRTDHETGNVAAVMDGDIEPFI
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHHCCCCCCCCCCEEEEECCCCCHHH
LAYLKTL
HHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA