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The map label for this gene is hacA [H]

Identifier: 45359043

GI number: 45359043

Start: 1440776

End: 1442032

Strand: Direct

Name: hacA [H]

Synonym: MMP1480

Alternate gene names: 45359043

Gene position: 1440776-1442032 (Clockwise)

Preceding gene: 45359042

Following gene: 45359044

Centisome position: 86.73

GC content: 38.35

Gene sequence:

>1257_bases
ATGACACTTGCTGAGAAAATCATTTCAAAAAATGTTGGAAAAAATGTTTACGCCAAAGACAGCGTCGAAATAAGCGTAGA
TATTGCAATGACACATGACGGGACCACCCCGCTTACGGTAAAAGCCTTTGAGCAGATTTCAGATAAAGTATGGGATAATG
AAAAGATAGTCATTATTTTTGACCACAATATCCCTGCAAACACGTCAAAAGCTGCAAATATGCAGGTTATAACGAGAGAA
TTCATAAAAAAGCAGGGGATTAAAAATTACTACCTCGATGGCGAAGGAATATGCCATCAAGTTCTACCTGAAAAGGGCCA
TGTAAAACCAAACATGATAATTGCGGGAGCTGACAGCCACACATGTACTCATGGGGCCTTTGGAGCATTTGCTACAGGTT
TTGGTGCTACAGACATGGGTTACGTCTATGCAACAGGAAAAACCTGGCTTAGAGTTCCTGAAACTATCCGCGTAAATGTA
ACTGGAGAAAATGAAAATATTTCTGGAAAAGACATTATTTTAAAAACTTGTAAGGAAGTTGGAAGACGTGGGGCTACGTA
CATGTCTTTAGAATACGGTGGAAATGCAGTCCACAATCTTTCAATGGATGAAAGAATGGTTCTGTCAAACATGGCTATTG
AAATGGGCGGAAAAGCAGGAATTATCGAAGCTGATGATACTACATATAGATATCTTGAAAATGCAGGAGTTTCGCGCGAA
GAAATTCTTGAATTGAAAAAAAATAAAATAACAGTTGATGAATCCGAAGAAGACTACTACAAAACAATTGAATTTGACAT
AACCGGTATGGAAGAACAGGTTGCATGCCCTCACCACCCTGATAACGTAAAAGGAGTTTCAGAAGTTGAAGGAACAGAAT
TAAACCAGGTATTCATCGGTTCATGCACAAACGGAAGATTAAACGACTTAAGAATTGCTGCAAAATATTTGAAAGGAAAA
AAAGTTAATGAAAACACAAGATTAATTGTAATCCCTGCATCAAAGTCAATATTTAAAGAAGCCCTAAATGAAGGATTAAT
TGATATCTTTGTAGATTCCGGAGCATTAATATGTACCCCTGGATGCGGACCATGTCTTGGAGCCCATCAGGGGGTTTTAG
GTGATGGAGAAGTATGCCTTGCTACAACTAACCGGAACTTTAAAGGAAGAATGGGAAACACGAACGCACAAGTTTACCTC
TCTTCTCCAAAAATAGCTGCAAAATCTGCGGTTAAAGGCTACATTACAAATGAATAA

Upstream 100 bases:

>100_bases
ATTTTAAAATTTAATTTAAATCCCTCATATTTATAAATCAATAACACCTAACCTATGAAGTACTGTTTTTATGTATATTA
CGAATTTACGGTGAGAATCT

Downstream 100 bases:

>100_bases
TAATCAATATTTGTTTCTCTTTTTTAAAATTAGTTTTATATAGTATTACCAACAGTATTCAAGATATATTTCATTTAGTA
AAATAATCTGGAATTAAATC

Product: aconitase family

Products: NA

Alternate protein names: HACN; (R)-homocitrate dehydratase; Homoaconitate hydratase [H]

Number of amino acids: Translated: 418; Mature: 417

Protein sequence:

>418_residues
MTLAEKIISKNVGKNVYAKDSVEISVDIAMTHDGTTPLTVKAFEQISDKVWDNEKIVIIFDHNIPANTSKAANMQVITRE
FIKKQGIKNYYLDGEGICHQVLPEKGHVKPNMIIAGADSHTCTHGAFGAFATGFGATDMGYVYATGKTWLRVPETIRVNV
TGENENISGKDIILKTCKEVGRRGATYMSLEYGGNAVHNLSMDERMVLSNMAIEMGGKAGIIEADDTTYRYLENAGVSRE
EILELKKNKITVDESEEDYYKTIEFDITGMEEQVACPHHPDNVKGVSEVEGTELNQVFIGSCTNGRLNDLRIAAKYLKGK
KVNENTRLIVIPASKSIFKEALNEGLIDIFVDSGALICTPGCGPCLGAHQGVLGDGEVCLATTNRNFKGRMGNTNAQVYL
SSPKIAAKSAVKGYITNE

Sequences:

>Translated_418_residues
MTLAEKIISKNVGKNVYAKDSVEISVDIAMTHDGTTPLTVKAFEQISDKVWDNEKIVIIFDHNIPANTSKAANMQVITRE
FIKKQGIKNYYLDGEGICHQVLPEKGHVKPNMIIAGADSHTCTHGAFGAFATGFGATDMGYVYATGKTWLRVPETIRVNV
TGENENISGKDIILKTCKEVGRRGATYMSLEYGGNAVHNLSMDERMVLSNMAIEMGGKAGIIEADDTTYRYLENAGVSRE
EILELKKNKITVDESEEDYYKTIEFDITGMEEQVACPHHPDNVKGVSEVEGTELNQVFIGSCTNGRLNDLRIAAKYLKGK
KVNENTRLIVIPASKSIFKEALNEGLIDIFVDSGALICTPGCGPCLGAHQGVLGDGEVCLATTNRNFKGRMGNTNAQVYL
SSPKIAAKSAVKGYITNE
>Mature_417_residues
TLAEKIISKNVGKNVYAKDSVEISVDIAMTHDGTTPLTVKAFEQISDKVWDNEKIVIIFDHNIPANTSKAANMQVITREF
IKKQGIKNYYLDGEGICHQVLPEKGHVKPNMIIAGADSHTCTHGAFGAFATGFGATDMGYVYATGKTWLRVPETIRVNVT
GENENISGKDIILKTCKEVGRRGATYMSLEYGGNAVHNLSMDERMVLSNMAIEMGGKAGIIEADDTTYRYLENAGVSREE
ILELKKNKITVDESEEDYYKTIEFDITGMEEQVACPHHPDNVKGVSEVEGTELNQVFIGSCTNGRLNDLRIAAKYLKGKK
VNENTRLIVIPASKSIFKEALNEGLIDIFVDSGALICTPGCGPCLGAHQGVLGDGEVCLATTNRNFKGRMGNTNAQVYLS
SPKIAAKSAVKGYITNE

Specific function: Hydro-lyase with broad substrate specificity for cis- unsaturated tricarboxylic acids. Catalyzes both the reversible dehydration of (R)-homocitrate ((R)-2-hydroxybutane-1,2,4- tricarboxylate) to produce cis-homoaconitate ((Z)-but-1-ene-1,2,4- tricarboxyla

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=358, Percent_Identity=28.4916201117318, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI41352693, Length=395, Percent_Identity=23.2911392405063, Blast_Score=83, Evalue=5e-16,
Organism=Homo sapiens, GI8659555, Length=414, Percent_Identity=22.463768115942, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1786259, Length=473, Percent_Identity=32.3467230443975, Blast_Score=199, Evalue=4e-52,
Organism=Escherichia coli, GI2367097, Length=434, Percent_Identity=24.6543778801843, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI87081781, Length=366, Percent_Identity=25.4098360655738, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787531, Length=406, Percent_Identity=22.1674876847291, Blast_Score=65, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI32564738, Length=371, Percent_Identity=28.8409703504043, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI25149337, Length=371, Percent_Identity=28.8409703504043, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI25149342, Length=287, Percent_Identity=29.616724738676, Blast_Score=106, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6320440, Length=425, Percent_Identity=30.3529411764706, Blast_Score=199, Evalue=5e-52,
Organism=Saccharomyces cerevisiae, GI6321429, Length=434, Percent_Identity=32.7188940092166, Blast_Score=196, Evalue=5e-51,
Organism=Saccharomyces cerevisiae, GI6322261, Length=372, Percent_Identity=29.8387096774194, Blast_Score=132, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6323335, Length=342, Percent_Identity=28.0701754385965, Blast_Score=132, Evalue=1e-31,
Organism=Drosophila melanogaster, GI28571643, Length=454, Percent_Identity=26.8722466960352, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI161076999, Length=376, Percent_Identity=27.1276595744681, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI281365315, Length=376, Percent_Identity=27.1276595744681, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI17864292, Length=376, Percent_Identity=27.1276595744681, Blast_Score=105, Evalue=7e-23,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR011826
- InterPro:   IPR015936
- InterPro:   IPR006251 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.36 [H]

Molecular weight: Translated: 45588; Mature: 45457

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLAEKIISKNVGKNVYAKDSVEISVDIAMTHDGTTPLTVKAFEQISDKVWDNEKIVIIF
CCHHHHHHHHCCCCCEEECCCEEEEEEEEEECCCCCCEEHHHHHHHHHHHCCCCEEEEEE
DHNIPANTSKAANMQVITREFIKKQGIKNYYLDGEGICHQVLPEKGHVKPNMIIAGADSH
CCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCHHHHCCCCCCCCCCEEEEECCCC
TCTHGAFGAFATGFGATDMGYVYATGKTWLRVPETIRVNVTGENENISGKDIILKTCKEV
CCCCCCCHHHHCCCCCCCCCEEEECCCEEEECCCEEEEEEECCCCCCCCHHHHHHHHHHH
GRRGATYMSLEYGGNAVHNLSMDERMVLSNMAIEMGGKAGIIEADDTTYRYLENAGVSRE
HCCCCEEEEEECCCCEEEECCCCHHHHHHHHHEEECCCEEEEECCCCHHHHHHHCCCCHH
EILELKKNKITVDESEEDYYKTIEFDITGMEEQVACPHHPDNVKGVSEVEGTELNQVFIG
HHHHHHHCEEEECCCCCCEEEEEEEEEECCHHHCCCCCCCCCCCCCCCCCCCCCCEEEEE
SCTNGRLNDLRIAAKYLKGKKVNENTRLIVIPASKSIFKEALNEGLIDIFVDSGALICTP
ECCCCCCHHHHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHCCEEEEEECCCCEEECC
GCGPCLGAHQGVLGDGEVCLATTNRNFKGRMGNTNAQVYLSSPKIAAKSAVKGYITNE
CCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCEEEEECCCCHHHHHHHCCEECCC
>Mature Secondary Structure 
TLAEKIISKNVGKNVYAKDSVEISVDIAMTHDGTTPLTVKAFEQISDKVWDNEKIVIIF
CHHHHHHHHCCCCCEEECCCEEEEEEEEEECCCCCCEEHHHHHHHHHHHCCCCEEEEEE
DHNIPANTSKAANMQVITREFIKKQGIKNYYLDGEGICHQVLPEKGHVKPNMIIAGADSH
CCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCHHHHCCCCCCCCCCEEEEECCCC
TCTHGAFGAFATGFGATDMGYVYATGKTWLRVPETIRVNVTGENENISGKDIILKTCKEV
CCCCCCCHHHHCCCCCCCCCEEEECCCEEEECCCEEEEEEECCCCCCCCHHHHHHHHHHH
GRRGATYMSLEYGGNAVHNLSMDERMVLSNMAIEMGGKAGIIEADDTTYRYLENAGVSRE
HCCCCEEEEEECCCCEEEECCCCHHHHHHHHHEEECCCEEEEECCCCHHHHHHHCCCCHH
EILELKKNKITVDESEEDYYKTIEFDITGMEEQVACPHHPDNVKGVSEVEGTELNQVFIG
HHHHHHHCEEEECCCCCCEEEEEEEEEECCHHHCCCCCCCCCCCCCCCCCCCCCCEEEEE
SCTNGRLNDLRIAAKYLKGKKVNENTRLIVIPASKSIFKEALNEGLIDIFVDSGALICTP
ECCCCCCHHHHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHCCEEEEEECCCCEEECC
GCGPCLGAHQGVLGDGEVCLATTNRNFKGRMGNTNAQVYLSSPKIAAKSAVKGYITNE
CCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCEEEEECCCCHHHHHHHCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]