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The map label for this gene is ehaO [C]

Identifier: 45359025

GI number: 45359025

Start: 1424070

End: 1425197

Strand: Direct

Name: ehaO [C]

Synonym: MMP1462

Alternate gene names: 45359025

Gene position: 1424070-1425197 (Clockwise)

Preceding gene: 45359024

Following gene: 45359026

Centisome position: 85.73

GC content: 36.08

Gene sequence:

>1128_bases
ATGAATGTAGTACCGATTGGACCCATTCACCCGATATTAAAAGAACCCTTGCGAATAAAACTCCTCGTTGAAGGAGAAAA
CGTAAAAGGTGCTGAAATAGACATGGGTTACGTTCACAGGGGTATTGAACGTATCATGGAAGGAAAACATTATTTAAAAT
GTATACACCTTTCAGAAAGAGTTTGCGGAATCTGTTCATATATCCACACCCAGTCCTTTGCAGAATGCATTGAAAATATG
TGTGATATTGAAGTTCCTGAAAAAGCAAAATATTTAAGAGTAATTACCTGCGAACTTGAGAGAATTCACAGCCACTTAAT
TGCAGCAGCAGTTTACAACCTTACAATTGAACATGAAACTCTTGGAATATGGATGTTAAATGTAAGGGAATCTATCATGG
ATTTACTCGAATTAATTACGGGAAATAGAGTAAACATGGGATTCAACGTTGTTGGTGGTGTAAGACTCGATTTAAACAAG
GAAATTTTAGATAAAATTCACAAAAAAATTGATGAATTTGAAGAAGATGTTAAAAATATAATTGAAGTATTCGAAACCGG
CCCATTAATTGGAATGAGAAGTAAACATATTGGAAAAATTGGCTACAAAGAAATCATGAAAACACGTGCAGCAGGACCTG
TTGCAAGAGCTTCAGGAATTCCTGAAAGTGACTGGAGAATAAGACATGAAACTTACAAAGCACTAAATTTCAAACCCGAT
TGGAGAAATGAAGGGGACAACTACGCACGAATGATGGTAAGACATGATGAAGTGCTCACAAGTGTCGAACTTATAAGAAG
AGCTCTTGAACACTATGCTGAATCTTCAGGAATCGTTAGAAATAAGGCTGAAATTAGGGCTACTGAAGGAGAATGGTATA
ACGAAGCTCACAGGGGCGAAGTTTACTATAAAATTGCAATTACCGATGGCGGACTCATAAAAAGAATTATTATAAGAACT
CCAACTGTAATGAACCTTGAAGCTTATAAATACATGATAAAAGACTGCCCAACAATTGCGGATGCAGTTGCAACATATAC
CTCGATAGACCCTTGTGTATCCTGCACTGAAAGAACTATCCAATTAAAAGACTTAAATACTGGAAAACTTAGTGACTACA
AAATCTAA

Upstream 100 bases:

>100_bases
TATCAATTGCCCCAGATGCACTTGCGGCTAAAGAAAAACTGCTTAATCAAAAATTAAAAGAAGAAAAAAATAAAGTCAAA
GCATGATTTGAGATGATATT

Downstream 100 bases:

>100_bases
TAAATAAACCTGATTGCAGGTGATACTTATGTCATCATCCATTTGGTACCTCTACGAGTTCGTAAGAAAAAAATGGTTTA
TGAGATTTACGAACGCCAAA

Product: energy conserving hydrogenase A large subunit

Products: NAD+; ubiquinol

Alternate protein names: NA

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MNVVPIGPIHPILKEPLRIKLLVEGENVKGAEIDMGYVHRGIERIMEGKHYLKCIHLSERVCGICSYIHTQSFAECIENM
CDIEVPEKAKYLRVITCELERIHSHLIAAAVYNLTIEHETLGIWMLNVRESIMDLLELITGNRVNMGFNVVGGVRLDLNK
EILDKIHKKIDEFEEDVKNIIEVFETGPLIGMRSKHIGKIGYKEIMKTRAAGPVARASGIPESDWRIRHETYKALNFKPD
WRNEGDNYARMMVRHDEVLTSVELIRRALEHYAESSGIVRNKAEIRATEGEWYNEAHRGEVYYKIAITDGGLIKRIIIRT
PTVMNLEAYKYMIKDCPTIADAVATYTSIDPCVSCTERTIQLKDLNTGKLSDYKI

Sequences:

>Translated_375_residues
MNVVPIGPIHPILKEPLRIKLLVEGENVKGAEIDMGYVHRGIERIMEGKHYLKCIHLSERVCGICSYIHTQSFAECIENM
CDIEVPEKAKYLRVITCELERIHSHLIAAAVYNLTIEHETLGIWMLNVRESIMDLLELITGNRVNMGFNVVGGVRLDLNK
EILDKIHKKIDEFEEDVKNIIEVFETGPLIGMRSKHIGKIGYKEIMKTRAAGPVARASGIPESDWRIRHETYKALNFKPD
WRNEGDNYARMMVRHDEVLTSVELIRRALEHYAESSGIVRNKAEIRATEGEWYNEAHRGEVYYKIAITDGGLIKRIIIRT
PTVMNLEAYKYMIKDCPTIADAVATYTSIDPCVSCTERTIQLKDLNTGKLSDYKI
>Mature_375_residues
MNVVPIGPIHPILKEPLRIKLLVEGENVKGAEIDMGYVHRGIERIMEGKHYLKCIHLSERVCGICSYIHTQSFAECIENM
CDIEVPEKAKYLRVITCELERIHSHLIAAAVYNLTIEHETLGIWMLNVRESIMDLLELITGNRVNMGFNVVGGVRLDLNK
EILDKIHKKIDEFEEDVKNIIEVFETGPLIGMRSKHIGKIGYKEIMKTRAAGPVARASGIPESDWRIRHETYKALNFKPD
WRNEGDNYARMMVRHDEVLTSVELIRRALEHYAESSGIVRNKAEIRATEGEWYNEAHRGEVYYKIAITDGGLIKRIIIRT
PTVMNLEAYKYMIKDCPTIADAVATYTSIDPCVSCTERTIQLKDLNTGKLSDYKI

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG3261

COG function: function code C; Ni,Fe-hydrogenase III large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 49 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI4758786, Length=268, Percent_Identity=25.7462686567164, Blast_Score=112, Evalue=4e-25,
Organism=Homo sapiens, GI260898743, Length=268, Percent_Identity=25.7462686567164, Blast_Score=112, Evalue=5e-25,
Organism=Escherichia coli, GI1789076, Length=360, Percent_Identity=31.9444444444444, Blast_Score=188, Evalue=4e-49,
Organism=Escherichia coli, GI1788832, Length=357, Percent_Identity=31.0924369747899, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI145693162, Length=393, Percent_Identity=24.1730279898219, Blast_Score=106, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17555284, Length=268, Percent_Identity=26.1194029850746, Blast_Score=118, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI17568379, Length=268, Percent_Identity=25.3731343283582, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24638644, Length=270, Percent_Identity=28.5185185185185, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI221459469, Length=272, Percent_Identity=26.8382352941176, Blast_Score=119, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001135
- InterPro:   IPR014029
- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00346 Complex1_49kDa; PF00374 NiFeSe_Hases [H]

EC number: 1.6.5.3

Molecular weight: Translated: 42881; Mature: 42881

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: PS00507 NI_HGENASE_L_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVVPIGPIHPILKEPLRIKLLVEGENVKGAEIDMGYVHRGIERIMEGKHYLKCIHLSER
CCCCCCCCCCHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCHHHEEHHHHHHH
VCGICSYIHTQSFAECIENMCDIEVPEKAKYLRVITCELERIHSHLIAAAVYNLTIEHET
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHEEEEECE
LGIWMLNVRESIMDLLELITGNRVNMGFNVVGGVRLDLNKEILDKIHKKIDEFEEDVKNI
EEEEEHHHHHHHHHHHHHHCCCCEEECEEEECCEEEECCHHHHHHHHHHHHHHHHHHHHH
IEVFETGPLIGMRSKHIGKIGYKEIMKTRAAGPVARASGIPESDWRIRHETYKALNFKPD
HHHHHCCCCCCCCCHHHCHHHHHHHHHHHCCCCHHHHCCCCCCCCEEHHHHHHHCCCCCC
WRNEGDNYARMMVRHDEVLTSVELIRRALEHYAESSGIVRNKAEIRATEGEWYNEAHRGE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCHHEEECCCCHHCCCCCCE
VYYKIAITDGGLIKRIIIRTPTVMNLEAYKYMIKDCPTIADAVATYTSIDPCVSCTERTI
EEEEEEEECCCHHHHHHHCCCCEECHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHCEE
QLKDLNTGKLSDYKI
EEEECCCCCCCCCCC
>Mature Secondary Structure
MNVVPIGPIHPILKEPLRIKLLVEGENVKGAEIDMGYVHRGIERIMEGKHYLKCIHLSER
CCCCCCCCCCHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCHHHEEHHHHHHH
VCGICSYIHTQSFAECIENMCDIEVPEKAKYLRVITCELERIHSHLIAAAVYNLTIEHET
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHEEEEECE
LGIWMLNVRESIMDLLELITGNRVNMGFNVVGGVRLDLNKEILDKIHKKIDEFEEDVKNI
EEEEEHHHHHHHHHHHHHHCCCCEEECEEEECCEEEECCHHHHHHHHHHHHHHHHHHHHH
IEVFETGPLIGMRSKHIGKIGYKEIMKTRAAGPVARASGIPESDWRIRHETYKALNFKPD
HHHHHCCCCCCCCCHHHCHHHHHHHHHHHCCCCHHHHCCCCCCCCEEHHHHHHHCCCCCC
WRNEGDNYARMMVRHDEVLTSVELIRRALEHYAESSGIVRNKAEIRATEGEWYNEAHRGE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCHHEEECCCCHHCCCCCCE
VYYKIAITDGGLIKRIIIRTPTVMNLEAYKYMIKDCPTIADAVATYTSIDPCVSCTERTI
EEEEEEEECCCHHHHHHHCCCCEECHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHCEE
QLKDLNTGKLSDYKI
EEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]