Click here to switch to the map view.

The map label for this gene is ehaN [C]

Identifier: 45359024

GI number: 45359024

Start: 1423585

End: 1424055

Strand: Direct

Name: ehaN [C]

Synonym: MMP1461

Alternate gene names: 45359024

Gene position: 1423585-1424055 (Clockwise)

Preceding gene: 45359023

Following gene: 45359025

Centisome position: 85.7

GC content: 39.92

Gene sequence:

>471_bases
ATGATTAAAGATTTTGTAAGAAAACGATCAATTAACGTCTGTCTCGTAAACACCGGCGGTTGCAACGGTTGCGATATCGA
AATAGTATCTTGCCTTGCCCCAAGGTACGATATTGAACAGTACGGAATTTATGTGCATAACAATCCAAGAGAAGCCGATG
TTTTAGTTGTAACCGGTCCTGTAACCGGAGCTTGGGAAAAAAGACTTCTTGAAATTTACGAAAAGACCCCTGAACCAAAA
ATAGTTATCGCAGTTGGTGCATGCGCACTTAGCGGTGGAATATTTAAAGAAGGACATATTCACGGGCCTGTTGATAAAGT
AATTCCTGTGGATGCAAAACTTCCAGGATGCCCTCCAAGACCTTCAGAAATCATATCTGCAGTGGTATCAATTGCCCCAG
ATGCACTTGCGGCTAAAGAAAAACTGCTTAATCAAAAATTAAAAGAAGAAAAAAATAAAGTCAAAGCATGA

Upstream 100 bases:

>100_bases
ATCAAAAAAAGACCGTACAAAGAAGCGCTTGAAGAAGGCAGGCTACTTACCGTCAAAATCCTGAAAGGTGAAGAATAGAC
TTAATTTGGAGATGATCTTG

Downstream 100 bases:

>100_bases
TTTGAGATGATATTATGAATGTAGTACCGATTGGACCCATTCACCCGATATTAAAAGAACCCTTGCGAATAAAACTCCTC
GTTGAAGGAGAAAACGTAAA

Product: energy conserving hydrogenase A small subunit

Products: NAD+; ubiquinol

Alternate protein names: NA

Number of amino acids: Translated: 156; Mature: 156

Protein sequence:

>156_residues
MIKDFVRKRSINVCLVNTGGCNGCDIEIVSCLAPRYDIEQYGIYVHNNPREADVLVVTGPVTGAWEKRLLEIYEKTPEPK
IVIAVGACALSGGIFKEGHIHGPVDKVIPVDAKLPGCPPRPSEIISAVVSIAPDALAAKEKLLNQKLKEEKNKVKA

Sequences:

>Translated_156_residues
MIKDFVRKRSINVCLVNTGGCNGCDIEIVSCLAPRYDIEQYGIYVHNNPREADVLVVTGPVTGAWEKRLLEIYEKTPEPK
IVIAVGACALSGGIFKEGHIHGPVDKVIPVDAKLPGCPPRPSEIISAVVSIAPDALAAKEKLLNQKLKEEKNKVKA
>Mature_156_residues
MIKDFVRKRSINVCLVNTGGCNGCDIEIVSCLAPRYDIEQYGIYVHNNPREADVLVVTGPVTGAWEKRLLEIYEKTPEPK
IVIAVGACALSGGIFKEGHIHGPVDKVIPVDAKLPGCPPRPSEIISAVVSIAPDALAAKEKLLNQKLKEEKNKVKA

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG3260

COG function: function code C; Ni,Fe-hydrogenase III small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=137, Percent_Identity=30.6569343065693, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1789074, Length=141, Percent_Identity=43.9716312056738, Blast_Score=118, Evalue=2e-28,
Organism=Escherichia coli, GI1788834, Length=142, Percent_Identity=40.1408450704225, Blast_Score=111, Evalue=2e-26,
Organism=Escherichia coli, GI1788624, Length=111, Percent_Identity=37.8378378378378, Blast_Score=84, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17509685, Length=115, Percent_Identity=38.2608695652174, Blast_Score=94, Evalue=4e-20,
Organism=Drosophila melanogaster, GI18859983, Length=116, Percent_Identity=37.0689655172414, Blast_Score=96, Evalue=9e-21,
Organism=Drosophila melanogaster, GI24642371, Length=116, Percent_Identity=37.0689655172414, Blast_Score=96, Evalue=9e-21,
Organism=Drosophila melanogaster, GI24651058, Length=116, Percent_Identity=36.2068965517241, Blast_Score=94, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: 1.6.5.3

Molecular weight: Translated: 16911; Mature: 16911

Theoretical pI: Translated: 8.12; Mature: 8.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKDFVRKRSINVCLVNTGGCNGCDIEIVSCLAPRYDIEQYGIYVHNNPREADVLVVTGP
CCHHHHHHCCCCEEEEECCCCCCCHHHHHHHHCCCCCCCCCCEEEECCCCCCEEEEEECC
VTGAWEKRLLEIYEKTPEPKIVIAVGACALSGGIFKEGHIHGPVDKVIPVDAKLPGCPPR
CCCHHHHHHHHHHHCCCCCEEEEEEEEEHHCCCCCCCCCCCCCCCCEEECCCCCCCCCCC
PSEIISAVVSIAPDALAAKEKLLNQKLKEEKNKVKA
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIKDFVRKRSINVCLVNTGGCNGCDIEIVSCLAPRYDIEQYGIYVHNNPREADVLVVTGP
CCHHHHHHCCCCEEEEECCCCCCCHHHHHHHHCCCCCCCCCCEEEECCCCCCEEEEEECC
VTGAWEKRLLEIYEKTPEPKIVIAVGACALSGGIFKEGHIHGPVDKVIPVDAKLPGCPPR
CCCHHHHHHHHHHHCCCCCEEEEEEEEEHHCCCCCCCCCCCCCCCCEEECCCCCCCCCCC
PSEIISAVVSIAPDALAAKEKLLNQKLKEEKNKVKA
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]