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The map label for this gene is fruA [H]

Identifier: 45358945

GI number: 45358945

Start: 1362470

End: 1363714

Strand: Direct

Name: fruA [H]

Synonym: MMP1382

Alternate gene names: 45358945

Gene position: 1362470-1363714 (Clockwise)

Preceding gene: 45358944

Following gene: 45358946

Centisome position: 82.02

GC content: 38.96

Gene sequence:

>1245_bases
GTGGCAGAACCTGTAACTATCAGCCCTACAACTAGACATGAAGGGCATGCTAAGCTAGTTTTGAATGTAGATGATGAAGG
GATTGTAACTAAGGCATTTTATCCAAATACAACCCCTGTAAGAGGTTTCGAAACCATGCTGCAAGGAAAAACAGCAGCAT
TTGGACCTATTGCAGTTATGAGAATTTGTGGTATATGTCAGGCAACCCACGGTATTGCTTCATGTGAGGCAATCGAAAAT
GCTATTGATATGGAAATACCAAAAGACGGTATGATATTAAGAGAATTATTAGGTTTAGGAAATAGGATGCATTCGCACCC
ATTACACCATTTGTTAATTGTAGATGACTTCTTAAAACCTGAAGAAGCAGGAACTTTAAGAGTAGAAGGAATTAAATTAA
TCCAGAAAATGAGAAAAGCTGGACAGATGGTTGTAGACATTACTGGTGGAGAAGGAATTCACCCTCCAAACTTGATGGTT
GGAGGAATGAGGACCAACATCACAGAAAGAGCAAAATCCAAACTCTACTATGCTTGTAGAGAATACGAAAAAGATTGTAA
TGCAATGTTAGAAGTATTGGAAATGTTAATGGAAAGATACCTAGATGAAATCGGAATTCCTGATTTAGGTAAACATAACT
TACCATACATTGCTACAGACTCAACATTTGGTAACAGAGATGCTATCAACTGGAAAGATGTAACAGAAGTACCTGCACAA
AGATACTATGCAGACTTCCCAGACATTGCACAAACCGCAACAAACCAGATCCCATTCTACAGAGGAAACCCTGCAGAAGG
TGGACCTAGAGCAAGAATGATTAAATACGGAAACTTCAAACCTGCTGGCGGAGCAATGGACCTTAACCTTGCGAGAGCTA
TGGAAAACTTCGAAGCTGTTAAACGATCATTGGAACTTATCGATGAATTAAACATTAACGGAACTGTAAGAGAAGTACCT
AACTTCTATAATGCAAAAGAAGAATTCGGAATAGGTGTTCACGAAGCACCAAGAGCTACCAACACCCACATGGCTAAAAT
GGGCAAAGATGGCAGAATTGAAAAATACAACATCATTGCTGCTTCAACGTGGAACTTCCCAGCTGTTGAAAAGGCTATTG
AAGGTTACCACCATCAATATGCGGAAGTAATTATGAGAGCTTACGACATATGAGCTTCATGTGCTACGCACGTCATCGTA
AAAGATGAACAGACTAAAAAAGTTGTTGAAATAAGAGAATTCTAA

Upstream 100 bases:

>100_bases
ATATATAATCCCTTATCTGCATTTTTAAAGAGGTATTCCTTTATATTATCCCCGAGAAAGAAAGTTTAATGTATGCATAT
TACCCTTGGAGGTGAATAAA

Downstream 100 bases:

>100_bases
ATTCCTGAAAAGGAAAGTCTTAGAAAGGGAACCTTAATGGACCTTGAGAGACACAAATTAACTTACGGTAATCATAAAAA
AAATCATTTCAACGGTATTG

Product: coenzyme F420-reducing hydrogenase subunit alpha

Products: NA

Alternate protein names: 8-hydroxy-5-deazaflavin-reducing hydrogenase subunit alpha; FRH [H]

Number of amino acids: Translated: 414; Mature: 413

Protein sequence:

>414_residues
MAEPVTISPTTRHEGHAKLVLNVDDEGIVTKAFYPNTTPVRGFETMLQGKTAAFGPIAVMRICGICQATHGIASCEAIEN
AIDMEIPKDGMILRELLGLGNRMHSHPLHHLLIVDDFLKPEEAGTLRVEGIKLIQKMRKAGQMVVDITGGEGIHPPNLMV
GGMRTNITERAKSKLYYACREYEKDCNAMLEVLEMLMERYLDEIGIPDLGKHNLPYIATDSTFGNRDAINWKDVTEVPAQ
RYYADFPDIAQTATNQIPFYRGNPAEGGPRARMIKYGNFKPAGGAMDLNLARAMENFEAVKRSLELIDELNINGTVREVP
NFYNAKEEFGIGVHEAPRATNTHMAKMGKDGRIEKYNIIAASTWNFPAVEKAIEGYHHQYAEVIMRAYDIUASCATHVIV
KDEQTKKVVEIREF

Sequences:

>Translated_414_residues
MAEPVTISPTTRHEGHAKLVLNVDDEGIVTKAFYPNTTPVRGFETMLQGKTAAFGPIAVMRICGICQATHGIASCEAIEN
AIDMEIPKDGMILRELLGLGNRMHSHPLHHLLIVDDFLKPEEAGTLRVEGIKLIQKMRKAGQMVVDITGGEGIHPPNLMV
GGMRTNITERAKSKLYYACREYEKDCNAMLEVLEMLMERYLDEIGIPDLGKHNLPYIATDSTFGNRDAINWKDVTEVPAQ
RYYADFPDIAQTATNQIPFYRGNPAEGGPRARMIKYGNFKPAGGAMDLNLARAMENFEAVKRSLELIDELNINGTVREVP
NFYNAKEEFGIGVHEAPRATNTHMAKMGKDGRIEKYNIIAASTWNFPAVEKAIEGYHHQYAEVIMRAYDI*ASCATHVIV
KDEQTKKVVEIREF
>Mature_413_residues
AEPVTISPTTRHEGHAKLVLNVDDEGIVTKAFYPNTTPVRGFETMLQGKTAAFGPIAVMRICGICQATHGIASCEAIENA
IDMEIPKDGMILRELLGLGNRMHSHPLHHLLIVDDFLKPEEAGTLRVEGIKLIQKMRKAGQMVVDITGGEGIHPPNLMVG
GMRTNITERAKSKLYYACREYEKDCNAMLEVLEMLMERYLDEIGIPDLGKHNLPYIATDSTFGNRDAINWKDVTEVPAQR
YYADFPDIAQTATNQIPFYRGNPAEGGPRARMIKYGNFKPAGGAMDLNLARAMENFEAVKRSLELIDELNINGTVREVPN
FYNAKEEFGIGVHEAPRATNTHMAKMGKDGRIEKYNIIAASTWNFPAVEKAIEGYHHQYAEVIMRAYDI*ASCATHVIVK
DEQTKKVVEIREF

Specific function: Reduces the physiological low-potential two-electron acceptor coenzyme F420, and the artificial one-electron acceptor methylviologen [H]

COG id: COG3259

COG function: function code C; Coenzyme F420-reducing hydrogenase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787207, Length=105, Percent_Identity=35.2380952380952, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1789368, Length=109, Percent_Identity=31.1926605504587, Blast_Score=73, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017682
- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: =1.12.98.1 [H]

Molecular weight: Translated: 46105; Mature: 45973

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS00507 NI_HGENASE_L_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEPVTISPTTRHEGHAKLVLNVDDEGIVTKAFYPNTTPVRGFETMLQGKTAAFGPIAVM
CCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEECCCCCCCHHHHHHHCCCCCCCHHHHHH
RICGICQATHGIASCEAIENAIDMEIPKDGMILRELLGLGNRMHSHPLHHLLIVDDFLKP
HHHHHHHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHCCCCCEEEEEHHHCCC
EEAGTLRVEGIKLIQKMRKAGQMVVDITGGEGIHPPNLMVGGMRTNITERAKSKLYYACR
CCCCEEEEHHHHHHHHHHHCCCEEEEECCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHH
EYEKDCNAMLEVLEMLMERYLDEIGIPDLGKHNLPYIATDSTFGNRDAINWKDVTEVPAQ
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCCCCCCHHHHCCHH
RYYADFPDIAQTATNQIPFYRGNPAEGGPRARMIKYGNFKPAGGAMDLNLARAMENFEAV
HHCCCCCHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHH
KRSLELIDELNINGTVREVPNFYNAKEEFGIGVHEAPRATNTHMAKMGKDGRIEKYNIIA
HHHHHHHHHCCCCCCHHHCCCHHCCHHHHCCCCCCCCCCCCHHHHHCCCCCCCEEEEEEE
ASTWNFPAVEKAIEGYHHQYAEVIMRAYDIASCATHVIVKDEQTKKVVEIREF
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHCC
>Mature Secondary Structure 
AEPVTISPTTRHEGHAKLVLNVDDEGIVTKAFYPNTTPVRGFETMLQGKTAAFGPIAVM
CCCCCCCCCCCCCCCEEEEEEECCCCCEEEEECCCCCCCHHHHHHHCCCCCCCHHHHHH
RICGICQATHGIASCEAIENAIDMEIPKDGMILRELLGLGNRMHSHPLHHLLIVDDFLKP
HHHHHHHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHCCCCCEEEEEHHHCCC
EEAGTLRVEGIKLIQKMRKAGQMVVDITGGEGIHPPNLMVGGMRTNITERAKSKLYYACR
CCCCEEEEHHHHHHHHHHHCCCEEEEECCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHH
EYEKDCNAMLEVLEMLMERYLDEIGIPDLGKHNLPYIATDSTFGNRDAINWKDVTEVPAQ
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCCCCCCHHHHCCHH
RYYADFPDIAQTATNQIPFYRGNPAEGGPRARMIKYGNFKPAGGAMDLNLARAMENFEAV
HHCCCCCHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHH
KRSLELIDELNINGTVREVPNFYNAKEEFGIGVHEAPRATNTHMAKMGKDGRIEKYNIIA
HHHHHHHHHCCCCCCHHHCCCHHCCHHHHCCCCCCCCCCCCHHHHHCCCCCCCEEEEEEE
ASTWNFPAVEKAIEGYHHQYAEVIMRAYDIASCATHVIVKDEQTKKVVEIREF
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]