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The map label for this gene is rnhB

Identifier: 45358937

GI number: 45358937

Start: 1355645

End: 1356373

Strand: Direct

Name: rnhB

Synonym: MMP1374

Alternate gene names: 45358937

Gene position: 1355645-1356373 (Clockwise)

Preceding gene: 45358935

Following gene: 45358938

Centisome position: 81.61

GC content: 30.18

Gene sequence:

>729_bases
ATGAGTGAGGATATTAATAATAATTTAAATAATTCGGATAATTCAAACGGATCTAACGAAAAAATAATAGTTGGACTCGA
TGAAGCTGGCCGTGGACCAGTACTTGGGCCAATGGTAATTGCTTCGGTAAAAATTGATGAAAAAAATCTCTACAAGTTAA
ATGATCTTGAATTAAAGGACAGTAAACAACTCTCCAAAAAAAAGCGTGAAGAACTCTATATAATAATAAATGAAATGTGC
GATGTTGAAAAGATTGTAATTGACCCTGAAACAATCGACAAGCAGATGGAAATTATAAACTTAAACAAAATAGAATTAAG
CGCTTTTTCAAAACTTTCAAATCATTTTATCAGGGAAAATGATAATATATCAATTTATATCGATGCTTGTAGTAGCAGTG
AACAGTCATTTTCAAATCAATTTAAAGCAAAACTTATTAATAAAAACGTTGAAATCATTGCTGAACACAAAGCTGATGAA
AATTACAAAATAGTGTCTGCTGCATCAATTATTGCTAAAGTAACTCGTGATAGAGTTATTGAAGAATATAAAGAAACTTT
TGGGGAAATTGGAAGCGGATATCCAAGTGACCCAAAAACTAAAAAATTTTTAAAAAATTACGTGTATGAAAACAGAACTC
TTCCAAAAATTGCGAGAAAGAGCTGGGCAACTTCAAAAAATCTGTTAAAGGAAATCGAAGAATCAAAAATATTTCAGTGG
GTGAAATAA

Upstream 100 bases:

>100_bases
TATGGTATTTAAAATAGAGTTTTTAGACCCGAAATAACGCATAAGTATATTCGTGTTACTGTTTTTTATATGTTTCCTGT
TTAGTAAATTACGTGATATA

Downstream 100 bases:

>100_bases
TGGTTAACAAAGAACTGATTATAGATGATCGTGGGAAAAAATACCTTTTAAATAAAAACCTCGATAATTTCGGAAACGAC
CTTGGTGTTGTTGATTTAGT

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 242; Mature: 241

Protein sequence:

>242_residues
MSEDINNNLNNSDNSNGSNEKIIVGLDEAGRGPVLGPMVIASVKIDEKNLYKLNDLELKDSKQLSKKKREELYIIINEMC
DVEKIVIDPETIDKQMEIINLNKIELSAFSKLSNHFIRENDNISIYIDACSSSEQSFSNQFKAKLINKNVEIIAEHKADE
NYKIVSAASIIAKVTRDRVIEEYKETFGEIGSGYPSDPKTKKFLKNYVYENRTLPKIARKSWATSKNLLKEIEESKIFQW
VK

Sequences:

>Translated_242_residues
MSEDINNNLNNSDNSNGSNEKIIVGLDEAGRGPVLGPMVIASVKIDEKNLYKLNDLELKDSKQLSKKKREELYIIINEMC
DVEKIVIDPETIDKQMEIINLNKIELSAFSKLSNHFIRENDNISIYIDACSSSEQSFSNQFKAKLINKNVEIIAEHKADE
NYKIVSAASIIAKVTRDRVIEEYKETFGEIGSGYPSDPKTKKFLKNYVYENRTLPKIARKSWATSKNLLKEIEESKIFQW
VK
>Mature_241_residues
SEDINNNLNNSDNSNGSNEKIIVGLDEAGRGPVLGPMVIASVKIDEKNLYKLNDLELKDSKQLSKKKREELYIIINEMCD
VEKIVIDPETIDKQMEIINLNKIELSAFSKLSNHFIRENDNISIYIDACSSSEQSFSNQFKAKLINKNVEIIAEHKADEN
YKIVSAASIIAKVTRDRVIEEYKETFGEIGSGYPSDPKTKKFLKNYVYENRTLPKIARKSWATSKNLLKEIEESKIFQWV
K

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Homo sapiens, GI38455391, Length=237, Percent_Identity=33.3333333333333, Blast_Score=118, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI17536263, Length=235, Percent_Identity=34.468085106383, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6324257, Length=238, Percent_Identity=34.0336134453782, Blast_Score=83, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24580635, Length=231, Percent_Identity=35.0649350649351, Blast_Score=121, Evalue=5e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_METMP (Q6LXH7)

Other databases:

- EMBL:   BX950229
- RefSeq:   NP_988494.1
- ProteinModelPortal:   Q6LXH7
- SMR:   Q6LXH7
- GeneID:   2762238
- GenomeReviews:   BX950229_GR
- KEGG:   mmp:MMP1374
- NMPDR:   fig|267377.1.peg.1374
- HOGENOM:   HBG584843
- OMA:   AEHKADE
- ProtClustDB:   PRK00015
- BioCyc:   MMAR267377:MMP1374-MONOMER
- BRENDA:   3.1.26.4
- GO:   GO:0005737
- HAMAP:   MF_00052_A
- InterPro:   IPR004649
- InterPro:   IPR001352
- InterPro:   IPR020787
- InterPro:   IPR023160
- InterPro:   IPR012337
- Gene3D:   G3DSA:1.10.10.460
- PANTHER:   PTHR10954
- TIGRFAMs:   TIGR00729

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 27671; Mature: 27540

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEDINNNLNNSDNSNGSNEKIIVGLDEAGRGPVLGPMVIASVKIDEKNLYKLNDLELKD
CCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCEEEEEEEECCCCCEEECCCCCCH
SKQLSKKKREELYIIINEMCDVEKIVIDPETIDKQMEIINLNKIELSAFSKLSNHFIREN
HHHHHHHHHHHEEEEEHHHCCCEEEEECHHHHHCHHHEEECCHHHHHHHHHHHHHHHCCC
DNISIYIDACSSSEQSFSNQFKAKLINKNVEIIAEHKADENYKIVSAASIIAKVTRDRVI
CCEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEHHHHHHHHHHHHHHHH
EEYKETFGEIGSGYPSDPKTKKFLKNYVYENRTLPKIARKSWATSKNLLKEIEESKIFQW
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VK
CC
>Mature Secondary Structure 
SEDINNNLNNSDNSNGSNEKIIVGLDEAGRGPVLGPMVIASVKIDEKNLYKLNDLELKD
CCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCEEEEEEEECCCCCEEECCCCCCH
SKQLSKKKREELYIIINEMCDVEKIVIDPETIDKQMEIINLNKIELSAFSKLSNHFIREN
HHHHHHHHHHHEEEEEHHHCCCEEEEECHHHHHCHHHEEECCHHHHHHHHHHHHHHHCCC
DNISIYIDACSSSEQSFSNQFKAKLINKNVEIIAEHKADENYKIVSAASIIAKVTRDRVI
CCEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEHHHHHHHHHHHHHHHH
EEYKETFGEIGSGYPSDPKTKKFLKNYVYENRTLPKIARKSWATSKNLLKEIEESKIFQW
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VK
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA