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The map label for this gene is rpoA2 [H]

Identifier: 45358927

GI number: 45358927

Start: 1345497

End: 1346657

Strand: Direct

Name: rpoA2 [H]

Synonym: MMP1364

Alternate gene names: 45358927

Gene position: 1345497-1346657 (Clockwise)

Preceding gene: 45358926

Following gene: 45358928

Centisome position: 81.0

GC content: 36.78

Gene sequence:

>1161_bases
ATGCAAATGGCCGATTTAGAAAAGAAATTGGAAAATTCTGTCCTACCACCATTGTTAAAAAGGGAACTTTCAGAAAAAAT
CTTAAGGGAAGAAATAAGTGAAGAATACCTCGTTGATGAAATCATAAGCGAAACTACAAGAGCATACGAAAGAACTCTCG
TAGAACCCGGAGAAGCTGTTGGTGTTGTTGCGGCACAGTCAATTGGTGAACCTGGTACGCAGATGACAATGAGAACGTTC
CACTATGCGGGGGTAGCGGAGTTAAACGTTACCCTTGGTCTTCCAAGAATGATTGAAATTGTAGATGCCAGAAAAGAACC
ATCTACCCCAACAATGACAATCTACTTAAATGATGAATTTAAGGGCAATAGGGAAAAAGCTGCAACAGTTGCAAAAAACA
TTGAAAGTACCAATGTTGAATCTGTTTCAGAAGATATCAGTGTAGATTTGGTAAATGAATGTATTACAATTATATTAAAT
AACCAGCAGCTCGAAAGCAGGGGACTAACTGTTCCTGATGTAATCGATGCAATAAAATCTAAAATGAAACTTAAAATTGA
AGATCACGAAAATGTTCTCAATTTAAAAATCAAAACCCCTTCATTAAAAGCATTAAGAAAAAGACTTCCAAAAGTTAGGG
CAATCCACTTAAAAGGTGTTCAAAACATCAAAAGGGTTATCATCAGAAAAGAAGTTGATGAATACATCCTCTACAGTGAA
GGTTCAAACATTAAAGAAGTCTTTGATATCGAAGGTGTTGATACAACAAAAACAACCACAAACAACATTGTTGAAATTCA
GGATGTTTTAGGTATCGAAGCTGCAAGAAATGCAATCATATATGAAATGGATGCAACCCTTGGAAATCAGGGTCTTACGG
TAGACAAAAGACACTTGATGATGGTAGCAGACCTTATGTGTACAGATGGAGTTGTAAAACCAATCGGTAGACATGGTATT
GGTGGTGAAAAAGCATCAGTTCTTGCAAGAGCAGCATTTGAGGAAACTGTAAAACACCTTTACTCTGCATCCATGAGAGG
ATATGTTGATGAACTCGGTGGAGTTGTTGAAAATATTATTGTTGGAAAACCAATCTCAATGGGTACAGGCTGTATCGACA
TATGCATCGACAAGAGTTACGAAGAAGGAAAAGAATTATAA

Upstream 100 bases:

>100_bases
AAGGTGTAGATTTAGATAAAATATTCACAAAAGTTAATTCAAGATATGAAAATTAATTATTTTCAATTTCTTAAATTAAA
ACTATTTCTAGGTGAAACAC

Downstream 100 bases:

>100_bases
TTTAATACTTTTTTAATTATTTTTAAGATAATCGTTTTTTTATACCGTTTATAACACTCACTTCGATCCGTTAATTATAA
TTAGAGAGTTCTTGGTTGAT

Product: DNA-directed RNA polymerase subunit A''

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 386; Mature: 386

Protein sequence:

>386_residues
MQMADLEKKLENSVLPPLLKRELSEKILREEISEEYLVDEIISETTRAYERTLVEPGEAVGVVAAQSIGEPGTQMTMRTF
HYAGVAELNVTLGLPRMIEIVDARKEPSTPTMTIYLNDEFKGNREKAATVAKNIESTNVESVSEDISVDLVNECITIILN
NQQLESRGLTVPDVIDAIKSKMKLKIEDHENVLNLKIKTPSLKALRKRLPKVRAIHLKGVQNIKRVIIRKEVDEYILYSE
GSNIKEVFDIEGVDTTKTTTNNIVEIQDVLGIEAARNAIIYEMDATLGNQGLTVDKRHLMMVADLMCTDGVVKPIGRHGI
GGEKASVLARAAFEETVKHLYSASMRGYVDELGGVVENIIVGKPISMGTGCIDICIDKSYEEGKEL

Sequences:

>Translated_386_residues
MQMADLEKKLENSVLPPLLKRELSEKILREEISEEYLVDEIISETTRAYERTLVEPGEAVGVVAAQSIGEPGTQMTMRTF
HYAGVAELNVTLGLPRMIEIVDARKEPSTPTMTIYLNDEFKGNREKAATVAKNIESTNVESVSEDISVDLVNECITIILN
NQQLESRGLTVPDVIDAIKSKMKLKIEDHENVLNLKIKTPSLKALRKRLPKVRAIHLKGVQNIKRVIIRKEVDEYILYSE
GSNIKEVFDIEGVDTTKTTTNNIVEIQDVLGIEAARNAIIYEMDATLGNQGLTVDKRHLMMVADLMCTDGVVKPIGRHGI
GGEKASVLARAAFEETVKHLYSASMRGYVDELGGVVENIIVGKPISMGTGCIDICIDKSYEEGKEL
>Mature_386_residues
MQMADLEKKLENSVLPPLLKRELSEKILREEISEEYLVDEIISETTRAYERTLVEPGEAVGVVAAQSIGEPGTQMTMRTF
HYAGVAELNVTLGLPRMIEIVDARKEPSTPTMTIYLNDEFKGNREKAATVAKNIESTNVESVSEDISVDLVNECITIILN
NQQLESRGLTVPDVIDAIKSKMKLKIEDHENVLNLKIKTPSLKALRKRLPKVRAIHLKGVQNIKRVIIRKEVDEYILYSE
GSNIKEVFDIEGVDTTKTTTNNIVEIQDVLGIEAARNAIIYEMDATLGNQGLTVDKRHLMMVADLMCTDGVVKPIGRHGI
GGEKASVLARAAFEETVKHLYSASMRGYVDELGGVVENIIVGKPISMGTGCIDICIDKSYEEGKEL

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates [H]

COG id: COG0086

COG function: function code K; DNA-directed RNA polymerase, beta' subunit/160 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase beta' chain family [H]

Homologues:

Organism=Homo sapiens, GI39725938, Length=356, Percent_Identity=39.8876404494382, Blast_Score=213, Evalue=2e-55,
Organism=Homo sapiens, GI4505939, Length=168, Percent_Identity=38.0952380952381, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI103471997, Length=146, Percent_Identity=37.6712328767123, Blast_Score=92, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI25145495, Length=346, Percent_Identity=37.5722543352601, Blast_Score=197, Evalue=1e-50,
Organism=Caenorhabditis elegans, GI71987878, Length=159, Percent_Identity=40.251572327044, Blast_Score=114, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI71998295, Length=65, Percent_Identity=60, Blast_Score=85, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6324690, Length=364, Percent_Identity=37.6373626373626, Blast_Score=203, Evalue=5e-53,
Organism=Saccharomyces cerevisiae, GI6320061, Length=426, Percent_Identity=31.4553990610329, Blast_Score=201, Evalue=1e-52,
Organism=Saccharomyces cerevisiae, GI6324917, Length=87, Percent_Identity=50.5747126436782, Blast_Score=88, Evalue=2e-18,
Organism=Drosophila melanogaster, GI281360912, Length=387, Percent_Identity=35.1421188630491, Blast_Score=199, Evalue=3e-51,
Organism=Drosophila melanogaster, GI17530899, Length=168, Percent_Identity=39.2857142857143, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI17647875, Length=184, Percent_Identity=34.2391304347826, Blast_Score=104, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007081
- InterPro:   IPR012757 [H]

Pfam domain/function: PF04998 RNA_pol_Rpb1_5 [H]

EC number: =2.7.7.6 [H]

Molecular weight: Translated: 42796; Mature: 42796

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQMADLEKKLENSVLPPLLKRELSEKILREEISEEYLVDEIISETTRAYERTLVEPGEAV
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
GVVAAQSIGEPGTQMTMRTFHYAGVAELNVTLGLPRMIEIVDARKEPSTPTMTIYLNDEF
HHHHHHHCCCCCCHHHHHHHHHCCEEEEEEEECCHHHHHHHHCCCCCCCCEEEEEECCCC
KGNREKAATVAKNIESTNVESVSEDISVDLVNECITIILNNQQLESRGLTVPDVIDAIKS
CCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHH
KMKLKIEDHENVLNLKIKTPSLKALRKRLPKVRAIHLKGVQNIKRVIIRKEVDEYILYSE
HHEEEEECCCCEEEEEEECCHHHHHHHHCCHHEEEHHHHHHHHHHHHHHHHHHHEEEECC
GSNIKEVFDIEGVDTTKTTTNNIVEIQDVLGIEAARNAIIYEMDATLGNQGLTVDKRHLM
CCCCHHHHCCCCCCCCCCCCCCCEEHHHHHHHHHHCCEEEEEECCCCCCCCCEECHHHHH
MVADLMCTDGVVKPIGRHGIGGEKASVLARAAFEETVKHLYSASMRGYVDELGGVVENII
HHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGKPISMGTGCIDICIDKSYEEGKEL
CCCCCCCCCCEEEEEECCCHHCCCCC
>Mature Secondary Structure
MQMADLEKKLENSVLPPLLKRELSEKILREEISEEYLVDEIISETTRAYERTLVEPGEAV
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
GVVAAQSIGEPGTQMTMRTFHYAGVAELNVTLGLPRMIEIVDARKEPSTPTMTIYLNDEF
HHHHHHHCCCCCCHHHHHHHHHCCEEEEEEEECCHHHHHHHHCCCCCCCCEEEEEECCCC
KGNREKAATVAKNIESTNVESVSEDISVDLVNECITIILNNQQLESRGLTVPDVIDAIKS
CCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHH
KMKLKIEDHENVLNLKIKTPSLKALRKRLPKVRAIHLKGVQNIKRVIIRKEVDEYILYSE
HHEEEEECCCCEEEEEEECCHHHHHHHHCCHHEEEHHHHHHHHHHHHHHHHHHHEEEECC
GSNIKEVFDIEGVDTTKTTTNNIVEIQDVLGIEAARNAIIYEMDATLGNQGLTVDKRHLM
CCCCHHHHCCCCCCCCCCCCCCCEEHHHHHHHHHHCCEEEEEECCCCCCCCCEECHHHHH
MVADLMCTDGVVKPIGRHGIGGEKASVLARAAFEETVKHLYSASMRGYVDELGGVVENII
HHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGKPISMGTGCIDICIDKSYEEGKEL
CCCCCCCCCCEEEEEECCCHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA