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The map label for this gene is rpoB1 [H]

Identifier: 45358925

GI number: 45358925

Start: 1340896

End: 1342731

Strand: Direct

Name: rpoB1 [H]

Synonym: MMP1362

Alternate gene names: 45358925

Gene position: 1340896-1342731 (Clockwise)

Preceding gene: 45358924

Following gene: 45358926

Centisome position: 80.72

GC content: 38.94

Gene sequence:

>1836_bases
TTGGAAAAGCAGACAAATGTATATGTTAACGGGAAATTGATCGATACCACACAAGACCCTGAAACACTTGTTAAAAACTT
AAGGGAAAACAGAAGAAGTGGAAAATTATCGCCAAGCACGTCAATTTCGTTCAATGAAGAAAGTAACGATGTCCACATTT
CAACCGATGGTGGAAGGGCTGTAAGACCATTGATTGTTGTTGAAAACGGTGCTTCAAAAGTAACAGAAGAAATACTCGAA
AAAATGGAATCTAAAGAATTAACTTTTTTAGATTTAGTAAATTCAGGAATTATTGAATATTTAGATGCTGAAGAAGAAGA
AAACGCAAGAATTGCAATGTATGAAGATGAAATAACCGAGGAAAACACGCACGTTGAAATTGACCCTCTCGTTATTTTAG
GTATCGGTGCAGGTGTTGCCCCATATCCAGAACACAATTCAGCTCCAAGGATTACAATGGCTGCTGCAATGAGTAAACAG
TCACTTGGAATTCCTATGGCAAACATAAAATGGAGAATGGATACAAGAGGACACTTATTACACTACCCGCAAGTTCCACT
TGTAAGAACAAAACACCAGGAAATCTTAGGATTTGATAAAAGACCTGCTGGACAGAACTTCGTTGTTGCAGTTATGAGTT
ACGAAGGCTACAACATGGAGGATGCGTTCATTATCAACAAAGCATCTCTTGAAAGAGGTCTTGGTAGAAGTTCATTCTTT
AGAAGCTACGAAAGCTTTGAAAAAAGATACCCTGGAGGACAGCTCGATAAATTCGAAGTTCCTGAAAAAGGAGTTAGAGG
TTACAGAGCTGAAGAAGCCTACAGAAACTTAGGTGACGATGGTTTAATCGACCTTGAATCCTCAGTAAAATCGGGAGATG
TAATTTTAGGAAAAACGTCACCTCCAAGATTTTTAGAAGAACAAGAAATCACCCTTCAAACAAAATCACAGAGAAGAGAC
ACTTCAGTTACGATTCGACACGGTGAAGAAGGAATCGTTGACCTCGTAATTTTAAGTGAAACAAAAGAAGGAAACAGACT
TGGAAAAGTTAGAGTTAGAGATTTAAGAATTCCTGAGTTTGGTGACAAATTTGCATCAAGACACGGTCAGAAAGGGGTTA
TTGGACTCATTGTTCCACAAGAAGACTTACCCTATACTGAAGATGGAGTAATTCCTGATTTAATTATCAACCCGCACGCG
ATCCCTTCAAGGATGACTATCGGACAGGTTCTTGAAATGATTGGTGGTAAAGTTGGTTCATTGGAATGCAGAAGAATCGA
CGGAACAATTTTCAGCGGCGAAAGCGAATGGAAATTAAGAGAAGGCCTTGAAAAATACGGCTTTGAACACCATGCAAAAG
AAGCAATGTATGATGGAAAATCCGGTAGAAAATTAGAGTGCGATATTTTCGTTGGAGTTGCATACTACCAGAAATTACAC
CACTTAGTTGCTGGAAAAATCCACGCAAGAAGCAGAGGACCAATCCAAGTTCTTACAAGACAGCCTACAGAAGGTAGAGC
AAGAGAAGGAGGTCTTAGGTTTGGGGAAATGGAAAGAGATGTGTTAGTTGCCCACGGTGCTGCAATGCTCCTTAAGGAAA
GACTTCTCGATGAATCAGACCCTCACGAAGACTACGTCTGTGCTAAATGTGGTGAAATTGCAATCTTCGATTACAAGAGA
GGCATGAAATTCTGTCCAGTTTGTGGTGAATCAGAAGACATTCAAGATAACAGAAAGATTCCACCTGTAAAAATTGCTTA
TGCATTCAAATTATTGTTGGATGAGCTTAAAAGTATGGGAATCGATCCAAAATTAGAATTAAAAGACAGAGCATAA

Upstream 100 bases:

>100_bases
GGCGACGATAGCGTAATCGAATTATTAAAAGAACTCGGGCTCACAAAGGAAAACTAAATTTAATATTGATCGAATTTAGT
CAATTAAGAAGGTGGATACC

Downstream 100 bases:

>100_bases
CTTAAATTATATTATTTTTTATTAAAAAGGTCTAAAACCACGGTGAAACATATGGATAGATTTGATGTTCCAAAGGAAAT
CGGAGATATTACATTTGGAT

Product: DNA-directed RNA polymerase subunit B'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 611; Mature: 611

Protein sequence:

>611_residues
MEKQTNVYVNGKLIDTTQDPETLVKNLRENRRSGKLSPSTSISFNEESNDVHISTDGGRAVRPLIVVENGASKVTEEILE
KMESKELTFLDLVNSGIIEYLDAEEEENARIAMYEDEITEENTHVEIDPLVILGIGAGVAPYPEHNSAPRITMAAAMSKQ
SLGIPMANIKWRMDTRGHLLHYPQVPLVRTKHQEILGFDKRPAGQNFVVAVMSYEGYNMEDAFIINKASLERGLGRSSFF
RSYESFEKRYPGGQLDKFEVPEKGVRGYRAEEAYRNLGDDGLIDLESSVKSGDVILGKTSPPRFLEEQEITLQTKSQRRD
TSVTIRHGEEGIVDLVILSETKEGNRLGKVRVRDLRIPEFGDKFASRHGQKGVIGLIVPQEDLPYTEDGVIPDLIINPHA
IPSRMTIGQVLEMIGGKVGSLECRRIDGTIFSGESEWKLREGLEKYGFEHHAKEAMYDGKSGRKLECDIFVGVAYYQKLH
HLVAGKIHARSRGPIQVLTRQPTEGRAREGGLRFGEMERDVLVAHGAAMLLKERLLDESDPHEDYVCAKCGEIAIFDYKR
GMKFCPVCGESEDIQDNRKIPPVKIAYAFKLLLDELKSMGIDPKLELKDRA

Sequences:

>Translated_611_residues
MEKQTNVYVNGKLIDTTQDPETLVKNLRENRRSGKLSPSTSISFNEESNDVHISTDGGRAVRPLIVVENGASKVTEEILE
KMESKELTFLDLVNSGIIEYLDAEEEENARIAMYEDEITEENTHVEIDPLVILGIGAGVAPYPEHNSAPRITMAAAMSKQ
SLGIPMANIKWRMDTRGHLLHYPQVPLVRTKHQEILGFDKRPAGQNFVVAVMSYEGYNMEDAFIINKASLERGLGRSSFF
RSYESFEKRYPGGQLDKFEVPEKGVRGYRAEEAYRNLGDDGLIDLESSVKSGDVILGKTSPPRFLEEQEITLQTKSQRRD
TSVTIRHGEEGIVDLVILSETKEGNRLGKVRVRDLRIPEFGDKFASRHGQKGVIGLIVPQEDLPYTEDGVIPDLIINPHA
IPSRMTIGQVLEMIGGKVGSLECRRIDGTIFSGESEWKLREGLEKYGFEHHAKEAMYDGKSGRKLECDIFVGVAYYQKLH
HLVAGKIHARSRGPIQVLTRQPTEGRAREGGLRFGEMERDVLVAHGAAMLLKERLLDESDPHEDYVCAKCGEIAIFDYKR
GMKFCPVCGESEDIQDNRKIPPVKIAYAFKLLLDELKSMGIDPKLELKDRA
>Mature_611_residues
MEKQTNVYVNGKLIDTTQDPETLVKNLRENRRSGKLSPSTSISFNEESNDVHISTDGGRAVRPLIVVENGASKVTEEILE
KMESKELTFLDLVNSGIIEYLDAEEEENARIAMYEDEITEENTHVEIDPLVILGIGAGVAPYPEHNSAPRITMAAAMSKQ
SLGIPMANIKWRMDTRGHLLHYPQVPLVRTKHQEILGFDKRPAGQNFVVAVMSYEGYNMEDAFIINKASLERGLGRSSFF
RSYESFEKRYPGGQLDKFEVPEKGVRGYRAEEAYRNLGDDGLIDLESSVKSGDVILGKTSPPRFLEEQEITLQTKSQRRD
TSVTIRHGEEGIVDLVILSETKEGNRLGKVRVRDLRIPEFGDKFASRHGQKGVIGLIVPQEDLPYTEDGVIPDLIINPHA
IPSRMTIGQVLEMIGGKVGSLECRRIDGTIFSGESEWKLREGLEKYGFEHHAKEAMYDGKSGRKLECDIFVGVAYYQKLH
HLVAGKIHARSRGPIQVLTRQPTEGRAREGGLRFGEMERDVLVAHGAAMLLKERLLDESDPHEDYVCAKCGEIAIFDYKR
GMKFCPVCGESEDIQDNRKIPPVKIAYAFKLLLDELKSMGIDPKLELKDRA

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. The B (B''+B' and beta) subunits have been implicated in DNA promoter recognition and also in nucleotide binding [H]

COG id: COG0085

COG function: function code K; DNA-directed RNA polymerase, beta subunit/140 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase beta chain family [H]

Homologues:

Organism=Homo sapiens, GI4505941, Length=616, Percent_Identity=43.1818181818182, Blast_Score=501, Evalue=1e-141,
Organism=Homo sapiens, GI238908505, Length=604, Percent_Identity=41.887417218543, Blast_Score=484, Evalue=1e-136,
Organism=Homo sapiens, GI238908503, Length=604, Percent_Identity=42.0529801324503, Blast_Score=483, Evalue=1e-136,
Organism=Homo sapiens, GI33469941, Length=529, Percent_Identity=35.3497164461248, Blast_Score=307, Evalue=2e-83,
Organism=Homo sapiens, GI212286172, Length=529, Percent_Identity=35.3497164461248, Blast_Score=307, Evalue=2e-83,
Organism=Escherichia coli, GI1790419, Length=301, Percent_Identity=34.21926910299, Blast_Score=135, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17552304, Length=618, Percent_Identity=41.2621359223301, Blast_Score=497, Evalue=1e-141,
Organism=Caenorhabditis elegans, GI25144348, Length=614, Percent_Identity=41.6938110749186, Blast_Score=482, Evalue=1e-136,
Organism=Caenorhabditis elegans, GI17506623, Length=572, Percent_Identity=32.5174825174825, Blast_Score=282, Evalue=4e-76,
Organism=Saccharomyces cerevisiae, GI6324781, Length=612, Percent_Identity=44.281045751634, Blast_Score=497, Evalue=1e-141,
Organism=Saccharomyces cerevisiae, GI6324725, Length=645, Percent_Identity=42.015503875969, Blast_Score=496, Evalue=1e-141,
Organism=Saccharomyces cerevisiae, GI6325267, Length=456, Percent_Identity=38.1578947368421, Blast_Score=286, Evalue=5e-78,
Organism=Drosophila melanogaster, GI17136444, Length=617, Percent_Identity=43.2739059967585, Blast_Score=507, Evalue=1e-143,
Organism=Drosophila melanogaster, GI17647877, Length=605, Percent_Identity=41.8181818181818, Blast_Score=479, Evalue=1e-135,
Organism=Drosophila melanogaster, GI17136446, Length=634, Percent_Identity=32.018927444795, Blast_Score=284, Evalue=1e-76,

Paralogues:

None

Copy number: 4233 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015712
- InterPro:   IPR007120
- InterPro:   IPR019969
- InterPro:   IPR007121
- InterPro:   IPR007646
- InterPro:   IPR007647
- InterPro:   IPR007641
- InterPro:   IPR014724 [H]

Pfam domain/function: PF04566 RNA_pol_Rpb2_4; PF04567 RNA_pol_Rpb2_5; PF00562 RNA_pol_Rpb2_6; PF04560 RNA_pol_Rpb2_7 [H]

EC number: =2.7.7.6 [H]

Molecular weight: Translated: 68573; Mature: 68573

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: PS01166 RNA_POL_BETA ; PS00572 GLYCOSYL_HYDROL_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKQTNVYVNGKLIDTTQDPETLVKNLRENRRSGKLSPSTSISFNEESNDVHISTDGGRA
CCCCCEEEECCEEEECCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCEEEEECCCCCE
VRPLIVVENGASKVTEEILEKMESKELTFLDLVNSGIIEYLDAEEEENARIAMYEDEITE
EEEEEEEECCHHHHHHHHHHHHHCCCEEEEHHHHCCHHHHHCCCCCCCCEEEEEECCCCC
ENTHVEIDPLVILGIGAGVAPYPEHNSAPRITMAAAMSKQSLGIPMANIKWRMDTRGHLL
CCCEEEECCEEEEEECCCCCCCCCCCCCCCEEEHHHHCHHHCCCCCCCEEEEECCCCCEE
HYPQVPLVRTKHQEILGFDKRPAGQNFVVAVMSYEGYNMEDAFIINKASLERGLGRSSFF
ECCCCCCEECCCHHHHCCCCCCCCCCEEEEEEEECCCCCCCEEEEEHHHHHHCCCHHHHH
RSYESFEKRYPGGQLDKFEVPEKGVRGYRAEEAYRNLGDDGLIDLESSVKSGDVILGKTS
HHHHHHHHHCCCCCCCCCCCCHHHCCCCCHHHHHHCCCCCCEEEECCCCCCCCEEEECCC
PPRFLEEQEITLQTKSQRRDTSVTIRHGEEGIVDLVILSETKEGNRLGKVRVRDLRIPEF
CCCCCCCCCEEEEECCCCCCCEEEEEECCCCEEEEEEEECCCCCCCCCCEEEEEECCCCH
GDKFASRHGQKGVIGLIVPQEDLPYTEDGVIPDLIINPHAIPSRMTIGQVLEMIGGKVGS
HHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHCCCCCC
LECRRIDGTIFSGESEWKLREGLEKYGFEHHAKEAMYDGKSGRKLECDIFVGVAYYQKLH
EEEEEECCEEECCCCCHHHHHHHHHCCCHHHHHHHHCCCCCCCEEEEEEEEHHHHHHHHH
HLVAGKIHARSRGPIQVLTRQPTEGRAREGGLRFGEMERDVLVAHGAAMLLKERLLDESD
HHHHHHEECCCCCCEEEEECCCCCCCCHHCCCCCCCCCCCEEEHHHHHHHHHHHHCCCCC
PHEDYVCAKCGEIAIFDYKRGMKFCPVCGESEDIQDNRKIPPVKIAYAFKLLLDELKSMG
CCCCCHHHCCCCEEEEEECCCCEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
IDPKLELKDRA
CCCCEECCCCC
>Mature Secondary Structure
MEKQTNVYVNGKLIDTTQDPETLVKNLRENRRSGKLSPSTSISFNEESNDVHISTDGGRA
CCCCCEEEECCEEEECCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCEEEEECCCCCE
VRPLIVVENGASKVTEEILEKMESKELTFLDLVNSGIIEYLDAEEEENARIAMYEDEITE
EEEEEEEECCHHHHHHHHHHHHHCCCEEEEHHHHCCHHHHHCCCCCCCCEEEEEECCCCC
ENTHVEIDPLVILGIGAGVAPYPEHNSAPRITMAAAMSKQSLGIPMANIKWRMDTRGHLL
CCCEEEECCEEEEEECCCCCCCCCCCCCCCEEEHHHHCHHHCCCCCCCEEEEECCCCCEE
HYPQVPLVRTKHQEILGFDKRPAGQNFVVAVMSYEGYNMEDAFIINKASLERGLGRSSFF
ECCCCCCEECCCHHHHCCCCCCCCCCEEEEEEEECCCCCCCEEEEEHHHHHHCCCHHHHH
RSYESFEKRYPGGQLDKFEVPEKGVRGYRAEEAYRNLGDDGLIDLESSVKSGDVILGKTS
HHHHHHHHHCCCCCCCCCCCCHHHCCCCCHHHHHHCCCCCCEEEECCCCCCCCEEEECCC
PPRFLEEQEITLQTKSQRRDTSVTIRHGEEGIVDLVILSETKEGNRLGKVRVRDLRIPEF
CCCCCCCCCEEEEECCCCCCCEEEEEECCCCEEEEEEEECCCCCCCCCCEEEEEECCCCH
GDKFASRHGQKGVIGLIVPQEDLPYTEDGVIPDLIINPHAIPSRMTIGQVLEMIGGKVGS
HHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHCCCCCC
LECRRIDGTIFSGESEWKLREGLEKYGFEHHAKEAMYDGKSGRKLECDIFVGVAYYQKLH
EEEEEECCEEECCCCCHHHHHHHHHCCCHHHHHHHHCCCCCCCEEEEEEEEHHHHHHHHH
HLVAGKIHARSRGPIQVLTRQPTEGRAREGGLRFGEMERDVLVAHGAAMLLKERLLDESD
HHHHHHEECCCCCCEEEEECCCCCCCCHHCCCCCCCCCCCEEEHHHHHHHHHHHHCCCCC
PHEDYVCAKCGEIAIFDYKRGMKFCPVCGESEDIQDNRKIPPVKIAYAFKLLLDELKSMG
CCCCCHHHCCCCEEEEEECCCCEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
IDPKLELKDRA
CCCCEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA