Click here to switch to the map view.

The map label for this gene is rpoB2 [H]

Identifier: 45358924

GI number: 45358924

Start: 1339353

End: 1340852

Strand: Direct

Name: rpoB2 [H]

Synonym: MMP1361

Alternate gene names: 45358924

Gene position: 1339353-1340852 (Clockwise)

Preceding gene: 45358923

Following gene: 45358925

Centisome position: 80.63

GC content: 37.2

Gene sequence:

>1500_bases
ATGGAATCCCGTGTCGTTGTTGATGCATTTTTTAAAGAAAACAGTTTAGTAAAACACCACATCGATTCCTACGATGATTT
TGTTAAGAATAAAATTCAACGAATCATTGATGAGGTAACTGGTGTTGAAACAGAAATTAAAGGAGGATACAAAGTATCCT
TTGGAAACGTGAGAGTTACAAAACCAATAAATAAGGAAGCTGACGGTTCTGTAAAACCAATAACACCTATGGAAGCAAGA
ATCAGAAACCTTGCATACTCTGCACCGCTTTACCTTGAAATGATACCAACAGTTGGTGAAGGTGAAGAAGAAAAAGTATT
ATCCCCTGTTGAAGTTTACATTGGAGAACTTCCAGTAATGCTTGGTTCAGAAATCTGCCATTTGTGCGGAAAATCTGCAG
AAGAATTGATAAAATACGGCGAAGATACACAAGATCCTTTAGGTTATTTTATTGTTAATGGTTCAGAAAAAGCAGTTGTT
GCTCAGGAAGACCTTATTCCTAACAGAATTATATGTGAAAAGGTAGAAAAAAACAACAAAGTTGTAGATATTGCAAAAGT
ATTTTCAACCAGACATGGATTTAGGGCATTATGTACTGTTGAAAGAAATCCAGATGGATTATTAAATGTTTCATTTCCAG
GAATGCCAAGTACAATTCCACTAGTTATATTAATGAGAGCACTTGGTGCAGAATCAGATAGGGAAATTATCGAATTAATT
TCAGACGAACCAACAGTTGTCATGCAGTTAATTGCAAACTTACAGGAAGCAAGAGAAGAGCATGCGATAAACACGACTGA
AGATGCACTCGAACACATCGGAAAAAGAGTAGCTCCAGGACAGCCTAAAGAGTACAAGTTAAAAAGAGCAGAAACAATTC
TTTGTAACTACCTATTGCCACACATGGGCGTAGAATCTGAAAAATTAGGCGCTAAATGTAAATATCTTGGAAGAATGGCT
AAAAATTCAATCGAACTTTACTTAGGATTAAGGGTTGAAGATGATAAGGACCATTATTCAAATAAAAGATTAAAATTAGC
TGGAGACTTAATGGAAGACTTATTCAGACATTCATTCAACCAGTTAATTAAAGATATCAAATACCAGCTCGAAAGACAGG
CAATTAGAAACAAAGAGCCTTCAATTCAAGCTGCAGTTAGAAGCGATGTTTTAACTGAAAGAATGAGACATGCAATGGCT
ACTGGAAACTGGGTAGGTGGAAGAACAGGGGTATCCCAGCTTCTCGATAGAACCAGTTACCTTGCAACAGTATCACAATT
AAGAAGAGTTGTATCTCCACTTTCAAGATCACAGCCTCACTTCGAAGCTAGGGACTTGCACGCAACCCAATGGGGTAAAA
TATGTCCATCCGAAACACCAGAAGGTCCAAACTGTGGTCTGGTAAAAAACCTTGCTGTAATGTGCAAAGTAACTACTGAC
GAAGGCGACGATAGCGTAATCGAATTATTAAAAGAACTCGGGCTCACAAAGGAAAACTAA

Upstream 100 bases:

>100_bases
AATAGCAACTTCACTCTAACTGCTGGTTAATACCTGCTAAATATTTTTAAAATTTAAAATAAGCTATTAGTCCTTTATCT
AAAATCTATGGGGGAAAATC

Downstream 100 bases:

>100_bases
ATTTAATATTGATCGAATTTAGTCAATTAAGAAGGTGGATACCTTGGAAAAGCAGACAAATGTATATGTTAACGGGAAAT
TGATCGATACCACACAAGAC

Product: DNA-directed RNA polymerase subunit beta''

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 499; Mature: 499

Protein sequence:

>499_residues
MESRVVVDAFFKENSLVKHHIDSYDDFVKNKIQRIIDEVTGVETEIKGGYKVSFGNVRVTKPINKEADGSVKPITPMEAR
IRNLAYSAPLYLEMIPTVGEGEEEKVLSPVEVYIGELPVMLGSEICHLCGKSAEELIKYGEDTQDPLGYFIVNGSEKAVV
AQEDLIPNRIICEKVEKNNKVVDIAKVFSTRHGFRALCTVERNPDGLLNVSFPGMPSTIPLVILMRALGAESDREIIELI
SDEPTVVMQLIANLQEAREEHAINTTEDALEHIGKRVAPGQPKEYKLKRAETILCNYLLPHMGVESEKLGAKCKYLGRMA
KNSIELYLGLRVEDDKDHYSNKRLKLAGDLMEDLFRHSFNQLIKDIKYQLERQAIRNKEPSIQAAVRSDVLTERMRHAMA
TGNWVGGRTGVSQLLDRTSYLATVSQLRRVVSPLSRSQPHFEARDLHATQWGKICPSETPEGPNCGLVKNLAVMCKVTTD
EGDDSVIELLKELGLTKEN

Sequences:

>Translated_499_residues
MESRVVVDAFFKENSLVKHHIDSYDDFVKNKIQRIIDEVTGVETEIKGGYKVSFGNVRVTKPINKEADGSVKPITPMEAR
IRNLAYSAPLYLEMIPTVGEGEEEKVLSPVEVYIGELPVMLGSEICHLCGKSAEELIKYGEDTQDPLGYFIVNGSEKAVV
AQEDLIPNRIICEKVEKNNKVVDIAKVFSTRHGFRALCTVERNPDGLLNVSFPGMPSTIPLVILMRALGAESDREIIELI
SDEPTVVMQLIANLQEAREEHAINTTEDALEHIGKRVAPGQPKEYKLKRAETILCNYLLPHMGVESEKLGAKCKYLGRMA
KNSIELYLGLRVEDDKDHYSNKRLKLAGDLMEDLFRHSFNQLIKDIKYQLERQAIRNKEPSIQAAVRSDVLTERMRHAMA
TGNWVGGRTGVSQLLDRTSYLATVSQLRRVVSPLSRSQPHFEARDLHATQWGKICPSETPEGPNCGLVKNLAVMCKVTTD
EGDDSVIELLKELGLTKEN
>Mature_499_residues
MESRVVVDAFFKENSLVKHHIDSYDDFVKNKIQRIIDEVTGVETEIKGGYKVSFGNVRVTKPINKEADGSVKPITPMEAR
IRNLAYSAPLYLEMIPTVGEGEEEKVLSPVEVYIGELPVMLGSEICHLCGKSAEELIKYGEDTQDPLGYFIVNGSEKAVV
AQEDLIPNRIICEKVEKNNKVVDIAKVFSTRHGFRALCTVERNPDGLLNVSFPGMPSTIPLVILMRALGAESDREIIELI
SDEPTVVMQLIANLQEAREEHAINTTEDALEHIGKRVAPGQPKEYKLKRAETILCNYLLPHMGVESEKLGAKCKYLGRMA
KNSIELYLGLRVEDDKDHYSNKRLKLAGDLMEDLFRHSFNQLIKDIKYQLERQAIRNKEPSIQAAVRSDVLTERMRHAMA
TGNWVGGRTGVSQLLDRTSYLATVSQLRRVVSPLSRSQPHFEARDLHATQWGKICPSETPEGPNCGLVKNLAVMCKVTTD
EGDDSVIELLKELGLTKEN

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. The B (B''+B' and beta) subunits have been implicated in DNA promoter recognition and also in nucleotide binding [H]

COG id: COG0085

COG function: function code K; DNA-directed RNA polymerase, beta subunit/140 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase beta chain family [H]

Homologues:

Organism=Homo sapiens, GI4505941, Length=527, Percent_Identity=34.3453510436433, Blast_Score=307, Evalue=1e-83,
Organism=Homo sapiens, GI238908503, Length=508, Percent_Identity=35.0393700787402, Blast_Score=275, Evalue=9e-74,
Organism=Homo sapiens, GI238908505, Length=464, Percent_Identity=35.3448275862069, Blast_Score=251, Evalue=2e-66,
Organism=Homo sapiens, GI33469941, Length=534, Percent_Identity=23.9700374531835, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI212286172, Length=432, Percent_Identity=24.7685185185185, Blast_Score=94, Evalue=3e-19,
Organism=Escherichia coli, GI1790419, Length=157, Percent_Identity=31.2101910828025, Blast_Score=82, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17552304, Length=542, Percent_Identity=34.1328413284133, Blast_Score=296, Evalue=1e-80,
Organism=Caenorhabditis elegans, GI25144348, Length=516, Percent_Identity=31.2015503875969, Blast_Score=245, Evalue=3e-65,
Organism=Caenorhabditis elegans, GI17506623, Length=517, Percent_Identity=26.1121856866538, Blast_Score=135, Evalue=6e-32,
Organism=Saccharomyces cerevisiae, GI6324725, Length=540, Percent_Identity=34.2592592592593, Blast_Score=282, Evalue=8e-77,
Organism=Saccharomyces cerevisiae, GI6324781, Length=502, Percent_Identity=31.2749003984064, Blast_Score=235, Evalue=1e-62,
Organism=Saccharomyces cerevisiae, GI6325267, Length=484, Percent_Identity=26.0330578512397, Blast_Score=115, Evalue=1e-26,
Organism=Drosophila melanogaster, GI17136444, Length=531, Percent_Identity=34.8399246704331, Blast_Score=310, Evalue=2e-84,
Organism=Drosophila melanogaster, GI17647877, Length=495, Percent_Identity=32.5252525252525, Blast_Score=231, Evalue=6e-61,
Organism=Drosophila melanogaster, GI17136446, Length=496, Percent_Identity=21.7741935483871, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: 4233 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015712
- InterPro:   IPR007644
- InterPro:   IPR007642
- InterPro:   IPR007645 [H]

Pfam domain/function: PF04563 RNA_pol_Rpb2_1; PF04561 RNA_pol_Rpb2_2; PF04565 RNA_pol_Rpb2_3 [H]

EC number: =2.7.7.6 [H]

Molecular weight: Translated: 55883; Mature: 55883

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESRVVVDAFFKENSLVKHHIDSYDDFVKNKIQRIIDEVTGVETEIKGGYKVSFGNVRVT
CCCCEEEEEHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCEEEEECCEEEE
KPINKEADGSVKPITPMEARIRNLAYSAPLYLEMIPTVGEGEEEKVLSPVEVYIGELPVM
CCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCHHHCCCHHHHHHHHHHHH
LGSEICHLCGKSAEELIKYGEDTQDPLGYFIVNGSEKAVVAQEDLIPNRIICEKVEKNNK
HHHHHHHHHCCCHHHHHHCCCCCCCCCEEEEECCCCCEEEEHHHCCCCHHHHHHHHCCCC
VVDIAKVFSTRHGFRALCTVERNPDGLLNVSFPGMPSTIPLVILMRALGAESDREIIELI
EEHHHHHHHCCCCCEEEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHH
SDEPTVVMQLIANLQEAREEHAINTTEDALEHIGKRVAPGQPKEYKLKRAETILCNYLLP
CCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
HMGVESEKLGAKCKYLGRMAKNSIELYLGLRVEDDKDHYSNKRLKLAGDLMEDLFRHSFN
CCCCCCHHHHHHHHHHHHHHCCCEEEEEEEEEECCCHHHCCCHHHHHHHHHHHHHHHHHH
QLIKDIKYQLERQAIRNKEPSIQAAVRSDVLTERMRHAMATGNWVGGRTGVSQLLDRTSY
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
LATVSQLRRVVSPLSRSQPHFEARDLHATQWGKICPSETPEGPNCGLVKNLAVMCKVTTD
HHHHHHHHHHHHHHHCCCCCCCHHHCCHHHCCCCCCCCCCCCCCCCHHHCEEEEEEEECC
EGDDSVIELLKELGLTKEN
CCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MESRVVVDAFFKENSLVKHHIDSYDDFVKNKIQRIIDEVTGVETEIKGGYKVSFGNVRVT
CCCCEEEEEHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCEEEEECCEEEE
KPINKEADGSVKPITPMEARIRNLAYSAPLYLEMIPTVGEGEEEKVLSPVEVYIGELPVM
CCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCHHHCCCHHHHHHHHHHHH
LGSEICHLCGKSAEELIKYGEDTQDPLGYFIVNGSEKAVVAQEDLIPNRIICEKVEKNNK
HHHHHHHHHCCCHHHHHHCCCCCCCCCEEEEECCCCCEEEEHHHCCCCHHHHHHHHCCCC
VVDIAKVFSTRHGFRALCTVERNPDGLLNVSFPGMPSTIPLVILMRALGAESDREIIELI
EEHHHHHHHCCCCCEEEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHH
SDEPTVVMQLIANLQEAREEHAINTTEDALEHIGKRVAPGQPKEYKLKRAETILCNYLLP
CCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
HMGVESEKLGAKCKYLGRMAKNSIELYLGLRVEDDKDHYSNKRLKLAGDLMEDLFRHSFN
CCCCCCHHHHHHHHHHHHHHCCCEEEEEEEEEECCCHHHCCCHHHHHHHHHHHHHHHHHH
QLIKDIKYQLERQAIRNKEPSIQAAVRSDVLTERMRHAMATGNWVGGRTGVSQLLDRTSY
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
LATVSQLRRVVSPLSRSQPHFEARDLHATQWGKICPSETPEGPNCGLVKNLAVMCKVTTD
HHHHHHHHHHHHHHHCCCCCCCHHHCCHHHCCCCCCCCCCCCCCCCHHHCEEEEEEEECC
EGDDSVIELLKELGLTKEN
CCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA