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The map label for this gene is thiI [H]

Identifier: 45358917

GI number: 45358917

Start: 1333713

End: 1334864

Strand: Direct

Name: thiI [H]

Synonym: MMP1354

Alternate gene names: 45358917

Gene position: 1333713-1334864 (Clockwise)

Preceding gene: 45358905

Following gene: 45358918

Centisome position: 80.29

GC content: 32.73

Gene sequence:

>1152_bases
TTGAAAAAATTTATTGTGAGATACGGAGAAATTGGAACAAAATCAAGACAGACCATGCACAGGTTTGAAAAATTACTTGC
AAAAAACATTTCCAAACTTGCAAAAAAACATGGGATTTCCGCAGAAGTTGAAATAATCCATACGCGATTGATTGTAGATG
TTCCTGAAGAAAATTTTGAAAAGATGAAGGAACTATTAAAAAAAGTACCTGGAATTGTATCATTTAGCCCTTGCTACTAC
ATAGATCCAGATATTGAACAGATCAAGAAAATTTCATCAGAACTTTTTGAAAAAGAAATTGAGCGTTACGAAAATAAAGA
AAACATTACTTTTAGAGTAAAAACCCAAAGACCTCAGAAAAAATTCCCGTTAACGTCACTTGAAGTAAATATGGAAGTGG
GGGGCCCAATTTCTGAAAAATACGGAATAAAAGTAGATTTAAAAAACCCTACAATTTCACTTGATATCGAAGTATTCAAC
GAATACGCTTACGTATTTGCAAAAAGAATCGAAGGAATCGGTGGAATCCCCGTTGGAACTCAAGGAAAAGTTATTGTTTT
ATTATCCGATGGAATCGATAGCCCTGTTTCTGCGTACATGATGGCAAAAAGGGGATGTAAACTTTTACTTTTACACATGA
AAACGTCAGATGAAGGGCTTGAAAAAACTCAAAAACTTGTTGATGTTTTAAGTGACTTTGATCCAGAAGCTAAATTTGTA
TACGTAGACTTTAATGAAGAACTTGCAAAAATTAAAGCCGAACTCACAGAAATTAATAGAGACAAATACACGTGTCTTTA
CTGCAAAAAATACATGCTTAAACTGGCTGAAAAACATGCTAAATGGCATAAATGTGATGCTATTGTAAATGGAGATAATA
TGGGGCAAGTTGCATCCCAAACTCTTAAAAATTTGCGAGTAATTAGTGAAGGAATTGACTACCCGATTTTAAGACCTTTG
ATAGGACTTGATAAAGTTGAAATTATGGATATTGCAAGAAAAATTGGAACTTTTGATATTTCTACAAGTAAAGAAGTAAG
CTGTTTTGCAGTTCCAAAACACCCAATAACAAACGCAACACCTGACGAAATGGAAAAAATTGAAGAACAACTTTTAAACT
TAAGAAATTGTAAAACTTGTAAAAATTGCTAA

Upstream 100 bases:

>100_bases
GTTATATAGAGAATAACTGTTTGAAAGTATTTATTTAGGATGTAGCTATTTTATTTAAATTCATTTATTATTGGTTTAAA
AACGGTTTTAGGTTGAGATA

Downstream 100 bases:

>100_bases
ATTAGGAGGTATATTTTGAATTCTAATCCGTTTGAATCGTATAGAGATATTAAAATAATGAACGAAAACCCGAAATACCC
TGTAAAAGGGGAAGGAATAA

Product: thiamine biosynthesis protein

Products: NA

Alternate protein names: Sulfur carrier protein ThiS sulfurtransferase; Thiamine biosynthesis protein thiI; tRNA 4-thiouridine synthase [H]

Number of amino acids: Translated: 383; Mature: 383

Protein sequence:

>383_residues
MKKFIVRYGEIGTKSRQTMHRFEKLLAKNISKLAKKHGISAEVEIIHTRLIVDVPEENFEKMKELLKKVPGIVSFSPCYY
IDPDIEQIKKISSELFEKEIERYENKENITFRVKTQRPQKKFPLTSLEVNMEVGGPISEKYGIKVDLKNPTISLDIEVFN
EYAYVFAKRIEGIGGIPVGTQGKVIVLLSDGIDSPVSAYMMAKRGCKLLLLHMKTSDEGLEKTQKLVDVLSDFDPEAKFV
YVDFNEELAKIKAELTEINRDKYTCLYCKKYMLKLAEKHAKWHKCDAIVNGDNMGQVASQTLKNLRVISEGIDYPILRPL
IGLDKVEIMDIARKIGTFDISTSKEVSCFAVPKHPITNATPDEMEKIEEQLLNLRNCKTCKNC

Sequences:

>Translated_383_residues
MKKFIVRYGEIGTKSRQTMHRFEKLLAKNISKLAKKHGISAEVEIIHTRLIVDVPEENFEKMKELLKKVPGIVSFSPCYY
IDPDIEQIKKISSELFEKEIERYENKENITFRVKTQRPQKKFPLTSLEVNMEVGGPISEKYGIKVDLKNPTISLDIEVFN
EYAYVFAKRIEGIGGIPVGTQGKVIVLLSDGIDSPVSAYMMAKRGCKLLLLHMKTSDEGLEKTQKLVDVLSDFDPEAKFV
YVDFNEELAKIKAELTEINRDKYTCLYCKKYMLKLAEKHAKWHKCDAIVNGDNMGQVASQTLKNLRVISEGIDYPILRPL
IGLDKVEIMDIARKIGTFDISTSKEVSCFAVPKHPITNATPDEMEKIEEQLLNLRNCKTCKNC
>Mature_383_residues
MKKFIVRYGEIGTKSRQTMHRFEKLLAKNISKLAKKHGISAEVEIIHTRLIVDVPEENFEKMKELLKKVPGIVSFSPCYY
IDPDIEQIKKISSELFEKEIERYENKENITFRVKTQRPQKKFPLTSLEVNMEVGGPISEKYGIKVDLKNPTISLDIEVFN
EYAYVFAKRIEGIGGIPVGTQGKVIVLLSDGIDSPVSAYMMAKRGCKLLLLHMKTSDEGLEKTQKLVDVLSDFDPEAKFV
YVDFNEELAKIKAELTEINRDKYTCLYCKKYMLKLAEKHAKWHKCDAIVNGDNMGQVASQTLKNLRVISEGIDYPILRPL
IGLDKVEIMDIARKIGTFDISTSKEVSCFAVPKHPITNATPDEMEKIEEQLLNLRNCKTCKNC

Specific function: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. Thi

COG id: COG0301

COG function: function code H; Thiamine biosynthesis ATP pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1786625, Length=376, Percent_Identity=31.6489361702128, Blast_Score=160, Evalue=1e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014729
- InterPro:   IPR003720
- InterPro:   IPR020536
- InterPro:   IPR004114 [H]

Pfam domain/function: PF02568 ThiI; PF02926 THUMP [H]

EC number: =2.8.1.4 [H]

Molecular weight: Translated: 43708; Mature: 43708

Theoretical pI: Translated: 8.44; Mature: 8.44

Prosite motif: PS51165 THUMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKFIVRYGEIGTKSRQTMHRFEKLLAKNISKLAKKHGISAEVEIIHTRLIVDVPEENFE
CCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEECCHHHHH
KMKELLKKVPGIVSFSPCYYIDPDIEQIKKISSELFEKEIERYENKENITFRVKTQRPQK
HHHHHHHHCCCCEEECCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCC
KFPLTSLEVNMEVGGPISEKYGIKVDLKNPTISLDIEVFNEYAYVFAKRIEGIGGIPVGT
CCCCEEEEEEEEECCCCCCCCCEEEEECCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCCC
QGKVIVLLSDGIDSPVSAYMMAKRGCKLLLLHMKTSDEGLEKTQKLVDVLSDFDPEAKFV
CCCEEEEEECCCCCHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCEEE
YVDFNEELAKIKAELTEINRDKYTCLYCKKYMLKLAEKHAKWHKCDAIVNGDNMGQVASQ
EEECCHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHCCHHHCCEEECCCCHHHHHHH
TLKNLRVISEGIDYPILRPLIGLDKVEIMDIARKIGTFDISTSKEVSCFAVPKHPITNAT
HHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHCCEECCCCCCEEEEECCCCCCCCCC
PDEMEKIEEQLLNLRNCKTCKNC
HHHHHHHHHHHHHHHCCCHHCCC
>Mature Secondary Structure
MKKFIVRYGEIGTKSRQTMHRFEKLLAKNISKLAKKHGISAEVEIIHTRLIVDVPEENFE
CCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEECCHHHHH
KMKELLKKVPGIVSFSPCYYIDPDIEQIKKISSELFEKEIERYENKENITFRVKTQRPQK
HHHHHHHHCCCCEEECCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCC
KFPLTSLEVNMEVGGPISEKYGIKVDLKNPTISLDIEVFNEYAYVFAKRIEGIGGIPVGT
CCCCEEEEEEEEECCCCCCCCCEEEEECCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCCC
QGKVIVLLSDGIDSPVSAYMMAKRGCKLLLLHMKTSDEGLEKTQKLVDVLSDFDPEAKFV
CCCEEEEEECCCCCHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCEEE
YVDFNEELAKIKAELTEINRDKYTCLYCKKYMLKLAEKHAKWHKCDAIVNGDNMGQVASQ
EEECCHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHCCHHHCCEEECCCCHHHHHHH
TLKNLRVISEGIDYPILRPLIGLDKVEIMDIARKIGTFDISTSKEVSCFAVPKHPITNAT
HHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHCCEECCCCCCEEEEECCCCCCCCCC
PDEMEKIEEQLLNLRNCKTCKNC
HHHHHHHHHHHHHHHCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]