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The map label for this gene is phoS2_3

Identifier: 41410270

GI number: 41410270

Start: 4645850

End: 4646974

Strand: Reverse

Name: phoS2_3

Synonym: MAP4172c

Alternate gene names: 41410270

Gene position: 4646974-4645850 (Counterclockwise)

Preceding gene: 41410285

Following gene: 41410249

Centisome position: 96.22

GC content: 69.16

Gene sequence:

>1125_bases
ATGCGCACCCGATCGGCGGTCATCGCCGGCGTGCTGATGGCAACCACCCTGGTGGTCTCGGCGTGCGGCGAGACGCCCGC
CAGCCTGCCCTACACCGCCGGCGCCAAGGTCGATTGCGGCGGCAAGCAGACCCTGTCCGCCAGCGGCTCCACCGCGCAGG
CCAACGCGATGACCCGGTTCATCGCCGCCTACCGGACCGCGTGCCCGGGACAGACGCTCGACTACACCGCCAACGGGTCG
GGCAACGGAATCGGTGACTTCCTCGCGGGCAGAACGGATTTCGCGGGGTCGGACACACCGTTGTCGGGCGACCAGTATGC
CGCCGCCAAGCGACGCTGCGGCGGAGCCGACGCCTGGAACCTGCCGGTGGTGTTCGGTCCGCTGGCCATCACCTACAACC
TGGCCGCGGTCGACTCGCTGGTGCTCGACGCGCCCACCCTGGCCAAGATCTTCAACGGCACCATCACCCGCTGGGACGAT
CCGGCCCTGGCCCTGCTCAACGCCTCCATGCCGGCCGAGGACATTCGCGTCGTCTACCGCAGCGACGGCTCGGGCACCAC
CGACAACTTCCAGGCCTACCTGCAGTCGGCCGCCGGCGGAGTGTGGAACAAGGGCGCGGGCAAGACCTTCAACGGCGGCG
TGGGCACCGGCGCCGTCGGCAACACCGGCACCGCCGCGGCCGTCAAGAGCACCGAGGGTGCCATCAGCTACAACGAATTG
TCCTTCGCGCTGCAGCAGGGCCTGTTCGCCGCCGAGATCAAGACGCCGGCGAGCCGCCGATCGCTGCGTCCGGTGCGGAT
CGGCACCGACATCTTCGGCAAGACGATCAAGGGCGCCCGGATCGTCGGCACCGGCAACGACCTGGTGCTCGACCTGTCGT
CGTTCTACAACCCCGCCCAGCCCGACGTCTACCCGATCGTGCTGGCCACCTACGAGATCGTGTGCTCGAAATACCCGGGC
TTCGACGTGGCCAAGGCGGTCAAGGCGTTCCTGCAGGCCGCCATCGGGCCCGGGCAGGTCGAGTTGGCCAGGACCGGCTA
CATCCCGCTGTCGGCGGACTTTCAAGCCAAGGTGTCCGGCGCCGTCGACGCGATCAGCTCACCGCAGGCGCCCAACCCGG
ACTGA

Upstream 100 bases:

>100_bases
CCCGGCGGGGACGGCGCACTCGTTCATCCGACGTTTGTTTGCCTGTTCATCCGGTCGCGGTACATCTCGGTACCCGGACT
GTCAAGGAGCCCTCGTCGTG

Downstream 100 bases:

>100_bases
CCGTCGCGGCTTCAGTCCCGCGAAAAGGCAAGGGCGATCTCGGTTTCCACGTCGCGATAGGGCCGGCCGGACAGGTCGAG
GGTGACCCGGGCGATGGTGC

Product: PhoS2_3

Products: ADP; phosphate [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: PBP 3; PstS-3; Antigen Ag88 [H]

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MRTRSAVIAGVLMATTLVVSACGETPASLPYTAGAKVDCGGKQTLSASGSTAQANAMTRFIAAYRTACPGQTLDYTANGS
GNGIGDFLAGRTDFAGSDTPLSGDQYAAAKRRCGGADAWNLPVVFGPLAITYNLAAVDSLVLDAPTLAKIFNGTITRWDD
PALALLNASMPAEDIRVVYRSDGSGTTDNFQAYLQSAAGGVWNKGAGKTFNGGVGTGAVGNTGTAAAVKSTEGAISYNEL
SFALQQGLFAAEIKTPASRRSLRPVRIGTDIFGKTIKGARIVGTGNDLVLDLSSFYNPAQPDVYPIVLATYEIVCSKYPG
FDVAKAVKAFLQAAIGPGQVELARTGYIPLSADFQAKVSGAVDAISSPQAPNPD

Sequences:

>Translated_374_residues
MRTRSAVIAGVLMATTLVVSACGETPASLPYTAGAKVDCGGKQTLSASGSTAQANAMTRFIAAYRTACPGQTLDYTANGS
GNGIGDFLAGRTDFAGSDTPLSGDQYAAAKRRCGGADAWNLPVVFGPLAITYNLAAVDSLVLDAPTLAKIFNGTITRWDD
PALALLNASMPAEDIRVVYRSDGSGTTDNFQAYLQSAAGGVWNKGAGKTFNGGVGTGAVGNTGTAAAVKSTEGAISYNEL
SFALQQGLFAAEIKTPASRRSLRPVRIGTDIFGKTIKGARIVGTGNDLVLDLSSFYNPAQPDVYPIVLATYEIVCSKYPG
FDVAKAVKAFLQAAIGPGQVELARTGYIPLSADFQAKVSGAVDAISSPQAPNPD
>Mature_374_residues
MRTRSAVIAGVLMATTLVVSACGETPASLPYTAGAKVDCGGKQTLSASGSTAQANAMTRFIAAYRTACPGQTLDYTANGS
GNGIGDFLAGRTDFAGSDTPLSGDQYAAAKRRCGGADAWNLPVVFGPLAITYNLAAVDSLVLDAPTLAKIFNGTITRWDD
PALALLNASMPAEDIRVVYRSDGSGTTDNFQAYLQSAAGGVWNKGAGKTFNGGVGTGAVGNTGTAAAVKSTEGAISYNEL
SFALQQGLFAAEIKTPASRRSLRPVRIGTDIFGKTIKGARIVGTGNDLVLDLSSFYNPAQPDVYPIVLATYEIVCSKYPG
FDVAKAVKAFLQAAIGPGQVELARTGYIPLSADFQAKVSGAVDAISSPQAPNPD

Specific function: Part of the ABC transporter complex pstSACB involved in phosphate import [H]

COG id: COG0226

COG function: function code P; ABC-type phosphate transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pstS family [H]

Homologues:

Organism=Escherichia coli, GI2367271, Length=277, Percent_Identity=31.7689530685921, Blast_Score=109, Evalue=3e-25,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005673
- InterPro:   IPR006059 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 38447; Mature: 38447

Theoretical pI: Translated: 7.78; Mature: 7.78

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTRSAVIAGVLMATTLVVSACGETPASLPYTAGAKVDCGGKQTLSASGSTAQANAMTRF
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCEEECCCCCCHHHHHHHHH
IAAYRTACPGQTLDYTANGSGNGIGDFLAGRTDFAGSDTPLSGDQYAAAKRRCGGADAWN
HHHHHHCCCCCCEEEECCCCCCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCC
LPVVFGPLAITYNLAAVDSLVLDAPTLAKIFNGTITRWDDPALALLNASMPAEDIRVVYR
CCEEEECCEEEEEHHHHHHHHHCCHHHHHHHCCCCEECCCCEEEEEECCCCCCCEEEEEE
SDGSGTTDNFQAYLQSAAGGVWNKGAGKTFNGGVGTGAVGNTGTAAAVKSTEGAISYNEL
CCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEHHHH
SFALQQGLFAAEIKTPASRRSLRPVRIGTDIFGKTIKGARIVGTGNDLVLDLSSFYNPAQ
HHHHHCCCEEEECCCCHHHCCCCCEEECHHHHCCCCCCCEEEECCCCEEEEHHHHCCCCC
PDVYPIVLATYEIVCSKYPGFDVAKAVKAFLQAAIGPGQVELARTGYIPLSADFQAKVSG
CCEEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCEEECCCCHHHHHH
AVDAISSPQAPNPD
HHHHCCCCCCCCCC
>Mature Secondary Structure
MRTRSAVIAGVLMATTLVVSACGETPASLPYTAGAKVDCGGKQTLSASGSTAQANAMTRF
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCEEECCCCCCHHHHHHHHH
IAAYRTACPGQTLDYTANGSGNGIGDFLAGRTDFAGSDTPLSGDQYAAAKRRCGGADAWN
HHHHHHCCCCCCEEEECCCCCCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCC
LPVVFGPLAITYNLAAVDSLVLDAPTLAKIFNGTITRWDDPALALLNASMPAEDIRVVYR
CCEEEECCEEEEEHHHHHHHHHCCHHHHHHHCCCCEECCCCEEEEEECCCCCCCEEEEEE
SDGSGTTDNFQAYLQSAAGGVWNKGAGKTFNGGVGTGAVGNTGTAAAVKSTEGAISYNEL
CCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEHHHH
SFALQQGLFAAEIKTPASRRSLRPVRIGTDIFGKTIKGARIVGTGNDLVLDLSSFYNPAQ
HHHHHCCCEEEECCCCHHHCCCCCEEECHHHHCCCCCCCEEEECCCCEEEEHHHHCCCCC
PDVYPIVLATYEIVCSKYPGFDVAKAVKAFLQAAIGPGQVELARTGYIPLSADFQAKVSG
CCEEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCEEECCCCHHHHHH
AVDAISSPQAPNPD
HHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; phosphate [Periplasm]; H2O [C]

Specific reaction: ATP + phosphate [Periplasm] + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]