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The map label for this gene is thiE

Identifier: 41409995

GI number: 41409995

Start: 4362235

End: 4362906

Strand: Reverse

Name: thiE

Synonym: MAP3897c

Alternate gene names: 41409995

Gene position: 4362906-4362235 (Counterclockwise)

Preceding gene: 41410000

Following gene: 41409993

Centisome position: 90.33

GC content: 77.83

Gene sequence:

>672_bases
GTGCATCAACGTCTGGCAACTCTGGCCGCCGCGCGGCTCTACCTGTGCACCGACGCCCGCCGGGAGCGCGGCGATCTGGC
CGAGTTCGCCGACGCCGCCCTGGCCGGCGGCGTGGACGTCATCCAGCTGCGCGACAAGGGGTCGCCCGGGGAGCAGCGGT
TCGGCCCGCTGGAGGCGCGCGACGAGCTGGCCGCCTGCGAGATCCTGGCCGACGCGGCCCGCCGGCACGGCGCCCTGTTC
GCCGTCAACGACCGCGCCGACATCGCCCGCGCGGCCGGCGCCGACGTGCTGCACCTGGGGCAGGGTGACCTGCCGCTGGA
GGTGGCGCGCGCATTCGTCGGGCCCGACGTGCTGCTCGGGCTGTCCAGCCACGACCGCGACCAGATGGCCGCGGCGGCGG
CGGGACCGGCCGACTACTTCTGCGTCGGGCCCTGCTGGCCCACGCCCACCAAACCGGGCCGCGCGGCGCCGGGCTTGGCG
CTGGTGCGGGCGGCCGCCGAGCTGCACACCGGCAAGCCGTGGTTCGCGATCGGCGGGATCGACGCGCAGCGGCTGCCCGA
GGTGCTCGACGCCGGGGCCCGCCGGGTCGTGGTGGTGCGGGCGATCACCGCGGCGGACGACCCCGCCGCGGCGGCCCGGC
GGCTCAGCTCGGCGCTGGCAGCAGCGCGCTGA

Upstream 100 bases:

>100_bases
ACGGTAAGGGCGGGCGCGCCCACTCTCAGACCCCGGTCCCGGGACTCCCGTGTCTAAAACGAGTTGTTCGGGTTCCACGC
TAGCGCGCTAGCGTCGCGGT

Downstream 100 bases:

>100_bases
TCCGGCGGCACAGCTGCGCGGACGCCTCGTCGGTGTCGGCCGGGTCGCCGGGCATGCACCAGACGAAGACGCCGTCCTCC
AGCTCGATGGTGCGGGGCAG

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 223; Mature: 223

Protein sequence:

>223_residues
MHQRLATLAAARLYLCTDARRERGDLAEFADAALAGGVDVIQLRDKGSPGEQRFGPLEARDELAACEILADAARRHGALF
AVNDRADIARAAGADVLHLGQGDLPLEVARAFVGPDVLLGLSSHDRDQMAAAAAGPADYFCVGPCWPTPTKPGRAAPGLA
LVRAAAELHTGKPWFAIGGIDAQRLPEVLDAGARRVVVVRAITAADDPAAAARRLSSALAAAR

Sequences:

>Translated_223_residues
MHQRLATLAAARLYLCTDARRERGDLAEFADAALAGGVDVIQLRDKGSPGEQRFGPLEARDELAACEILADAARRHGALF
AVNDRADIARAAGADVLHLGQGDLPLEVARAFVGPDVLLGLSSHDRDQMAAAAAGPADYFCVGPCWPTPTKPGRAAPGLA
LVRAAAELHTGKPWFAIGGIDAQRLPEVLDAGARRVVVVRAITAADDPAAAARRLSSALAAAR
>Mature_223_residues
MHQRLATLAAARLYLCTDARRERGDLAEFADAALAGGVDVIQLRDKGSPGEQRFGPLEARDELAACEILADAARRHGALF
AVNDRADIARAAGADVLHLGQGDLPLEVARAFVGPDVLLGLSSHDRDQMAAAAAGPADYFCVGPCWPTPTKPGRAAPGLA
LVRAAAELHTGKPWFAIGGIDAQRLPEVLDAGARRVVVVRAITAADDPAAAARRLSSALAAAR

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=186, Percent_Identity=33.3333333333333, Blast_Score=71, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6325042, Length=233, Percent_Identity=30.0429184549356, Blast_Score=80, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 23207; Mature: 23207

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHQRLATLAAARLYLCTDARRERGDLAEFADAALAGGVDVIQLRDKGSPGEQRFGPLEAR
CCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEEEECCCCCCHHHCCCCCHH
DELAACEILADAARRHGALFAVNDRADIARAAGADVLHLGQGDLPLEVARAFVGPDVLLG
HHHHHHHHHHHHHHHCCCEEEECCHHHHHHHCCCCEEEECCCCCHHHHHHHHHCHHHEEE
LSSHDRDQMAAAAAGPADYFCVGPCWPTPTKPGRAAPGLALVRAAAELHTGKPWFAIGGI
CCCCCHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCC
DAQRLPEVLDAGARRVVVVRAITAADDPAAAARRLSSALAAAR
CHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MHQRLATLAAARLYLCTDARRERGDLAEFADAALAGGVDVIQLRDKGSPGEQRFGPLEAR
CCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEEEECCCCCCHHHCCCCCHH
DELAACEILADAARRHGALFAVNDRADIARAAGADVLHLGQGDLPLEVARAFVGPDVLLG
HHHHHHHHHHHHHHHCCCEEEECCHHHHHHHCCCCEEEECCCCCHHHHHHHHHCHHHEEE
LSSHDRDQMAAAAAGPADYFCVGPCWPTPTKPGRAAPGLALVRAAAELHTGKPWFAIGGI
CCCCCHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCC
DAQRLPEVLDAGARRVVVVRAITAADDPAAAARRLSSALAAAR
CHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA