Click here to switch to the map view.

The map label for this gene is baiB [H]

Identifier: 41408453

GI number: 41408453

Start: 2646296

End: 2646964

Strand: Direct

Name: baiB [H]

Synonym: MAP2355

Alternate gene names: 41408453

Gene position: 2646296-2646964 (Clockwise)

Preceding gene: 41408452

Following gene: 41408454

Centisome position: 54.79

GC content: 71.0

Gene sequence:

>669_bases
GTGATGCCGCCATCGCTGCTGCACACCTGGTTCGAGCTGCTGTCCCCCGAGCAGATCGTGATGGCCTACGGCATGACCGA
AAACCTGGGGCTGACCGCGCTGCGCGGCGACGAGTGGCTCGAACACCCGGGCAGCGTCGGGCGTGGTTTCCGCGACACCG
AGATCCGCATCCTCGACTCCCAGCAGCGACCGCTGGGCCCCGGCGAGGACGGCGACGTGTACCTGCGGGCGCCGATGAGC
GCGGGGTACCGCTACCTGGGTGGGGCGCCCCCGCTGCCGTCGACCGAGGACGGCTTCCGCTCCGCCGGGGACATCGGCCA
CCTCGACGAGGACGGCTACCTCTACATCGTCGACCGCCGGGTCGACATGATCATCAGCGGCGGCGCCAACGTGTTTCCGG
CCGAAGTCGAGTCCGCCCTGGCCGGCCATCCCGCAATCGCCGACGTCGTCGTGATCGGGCTGGCCGACCCGCAGTGGGGG
CGCCGGGTGCATGCGGTGGTCCAGCGCGCCGACGGCGCGTCGCTGACCGAGCAGCAGGTGATCGACTACGCCAAGGGCCG
GCTGGCCCCGTATAAGGCGCCCAAGACCGTCGAATTCGTCGACGCCATCCCACGCACGGCGGCGACCAAGGTCAACCGCT
CGGCGATGATCGCGGCCCGGGGCGGCTGA

Upstream 100 bases:

>100_bases
ACTTCACCGCCACACCCACCATGCTGGCCCGCATCGCAGCCCGGCCCGATGTCCGGCAGCGCGACCTGTCCAGCATCGTG
TTCATTCTGCAGGGGCCGCG

Downstream 100 bases:

>100_bases
CCGGCGTCGACGGGCTCCGCGGTTCCGCCGCCCAGCGCGGTAACCTCATTCTCAGAGCGCCGGCAGCGCGCGACGGAAGG
GACCAACCGCTCATGGCCGA

Product: hypothetical protein

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 222; Mature: 222

Protein sequence:

>222_residues
MMPPSLLHTWFELLSPEQIVMAYGMTENLGLTALRGDEWLEHPGSVGRGFRDTEIRILDSQQRPLGPGEDGDVYLRAPMS
AGYRYLGGAPPLPSTEDGFRSAGDIGHLDEDGYLYIVDRRVDMIISGGANVFPAEVESALAGHPAIADVVVIGLADPQWG
RRVHAVVQRADGASLTEQQVIDYAKGRLAPYKAPKTVEFVDAIPRTAATKVNRSAMIAARGG

Sequences:

>Translated_222_residues
MMPPSLLHTWFELLSPEQIVMAYGMTENLGLTALRGDEWLEHPGSVGRGFRDTEIRILDSQQRPLGPGEDGDVYLRAPMS
AGYRYLGGAPPLPSTEDGFRSAGDIGHLDEDGYLYIVDRRVDMIISGGANVFPAEVESALAGHPAIADVVVIGLADPQWG
RRVHAVVQRADGASLTEQQVIDYAKGRLAPYKAPKTVEFVDAIPRTAATKVNRSAMIAARGG
>Mature_222_residues
MMPPSLLHTWFELLSPEQIVMAYGMTENLGLTALRGDEWLEHPGSVGRGFRDTEIRILDSQQRPLGPGEDGDVYLRAPMS
AGYRYLGGAPPLPSTEDGFRSAGDIGHLDEDGYLYIVDRRVDMIISGGANVFPAEVESALAGHPAIADVVVIGLADPQWG
RRVHAVVQRADGASLTEQQVIDYAKGRLAPYKAPKTVEFVDAIPRTAATKVNRSAMIAARGG

Specific function: Active with a large number of C-24 bile acids possessing unhindered C-24 carboxyl groups. Requires ATP and produces AMP and pyrophosphate in addition to the bile-acid CoA thioester [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=220, Percent_Identity=30.9090909090909, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI122937307, Length=225, Percent_Identity=32.8888888888889, Blast_Score=105, Evalue=2e-23,
Organism=Homo sapiens, GI42544132, Length=209, Percent_Identity=33.4928229665072, Blast_Score=104, Evalue=6e-23,
Organism=Homo sapiens, GI38505220, Length=190, Percent_Identity=33.1578947368421, Blast_Score=98, Evalue=7e-21,
Organism=Homo sapiens, GI115511026, Length=191, Percent_Identity=32.9842931937173, Blast_Score=98, Evalue=7e-21,
Organism=Homo sapiens, GI58082049, Length=206, Percent_Identity=33.495145631068, Blast_Score=96, Evalue=2e-20,
Organism=Homo sapiens, GI187761345, Length=241, Percent_Identity=32.7800829875519, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI187761343, Length=241, Percent_Identity=32.7800829875519, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI157311624, Length=205, Percent_Identity=33.1707317073171, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI157311622, Length=205, Percent_Identity=33.1707317073171, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI13375727, Length=197, Percent_Identity=25.8883248730964, Blast_Score=75, Evalue=6e-14,
Organism=Escherichia coli, GI1788107, Length=208, Percent_Identity=31.7307692307692, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI221142682, Length=204, Percent_Identity=31.3725490196078, Blast_Score=107, Evalue=4e-25,
Organism=Escherichia coli, GI145693145, Length=166, Percent_Identity=33.7349397590361, Blast_Score=95, Evalue=4e-21,
Organism=Escherichia coli, GI1786810, Length=169, Percent_Identity=28.4023668639053, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1790505, Length=181, Percent_Identity=26.5193370165746, Blast_Score=69, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17559526, Length=205, Percent_Identity=39.0243902439024, Blast_Score=111, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17558820, Length=212, Percent_Identity=31.1320754716981, Blast_Score=101, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17557194, Length=210, Percent_Identity=32.3809523809524, Blast_Score=100, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI17531443, Length=214, Percent_Identity=34.1121495327103, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI32563687, Length=204, Percent_Identity=33.3333333333333, Blast_Score=96, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17538037, Length=199, Percent_Identity=29.6482412060301, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17560140, Length=209, Percent_Identity=30.1435406698565, Blast_Score=88, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI71994690, Length=220, Percent_Identity=28.1818181818182, Blast_Score=84, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI71994703, Length=220, Percent_Identity=28.1818181818182, Blast_Score=84, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI71994694, Length=220, Percent_Identity=28.1818181818182, Blast_Score=84, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17560308, Length=220, Percent_Identity=29.0909090909091, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI71985884, Length=201, Percent_Identity=30.3482587064677, Blast_Score=74, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI71996755, Length=206, Percent_Identity=26.2135922330097, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6319699, Length=202, Percent_Identity=30.1980198019802, Blast_Score=97, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24581924, Length=225, Percent_Identity=31.1111111111111, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI21358303, Length=209, Percent_Identity=31.1004784688995, Blast_Score=100, Evalue=7e-22,
Organism=Drosophila melanogaster, GI20130357, Length=206, Percent_Identity=33.0097087378641, Blast_Score=98, Evalue=3e-21,
Organism=Drosophila melanogaster, GI281365686, Length=208, Percent_Identity=30.7692307692308, Blast_Score=98, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24648253, Length=206, Percent_Identity=31.5533980582524, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24648255, Length=206, Percent_Identity=31.5533980582524, Blast_Score=95, Evalue=4e-20,
Organism=Drosophila melanogaster, GI21356947, Length=195, Percent_Identity=33.8461538461538, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24653035, Length=208, Percent_Identity=29.3269230769231, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI21356441, Length=207, Percent_Identity=31.4009661835749, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI19922652, Length=214, Percent_Identity=29.9065420560748, Blast_Score=91, Evalue=7e-19,
Organism=Drosophila melanogaster, GI24648260, Length=205, Percent_Identity=31.7073170731707, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24648257, Length=206, Percent_Identity=33.495145631068, Blast_Score=88, Evalue=5e-18,
Organism=Drosophila melanogaster, GI161076582, Length=204, Percent_Identity=28.921568627451, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24656500, Length=221, Percent_Identity=28.0542986425339, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI21355181, Length=198, Percent_Identity=31.3131313131313, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI18859661, Length=199, Percent_Identity=31.6582914572864, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 23967; Mature: 23967

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMPPSLLHTWFELLSPEQIVMAYGMTENLGLTALRGDEWLEHPGSVGRGFRDTEIRILDS
CCCHHHHHHHHHHCCCCCEEEEECCCCCCCEEEECCCHHHHCCCCCCCCCCCCEEEEEEC
QQRPLGPGEDGDVYLRAPMSAGYRYLGGAPPLPSTEDGFRSAGDIGHLDEDGYLYIVDRR
CCCCCCCCCCCCEEEECCHHCCCEECCCCCCCCCCCCHHHCCCCCCCCCCCCEEEEEECC
VDMIISGGANVFPAEVESALAGHPAIADVVVIGLADPQWGRRVHAVVQRADGASLTEQQV
EEEEEECCCCCCHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHH
IDYAKGRLAPYKAPKTVEFVDAIPRTAATKVNRSAMIAARGG
HHHHHCCCCCCCCCCCHHHHHHCCHHHHHHCCCEEEEEECCC
>Mature Secondary Structure
MMPPSLLHTWFELLSPEQIVMAYGMTENLGLTALRGDEWLEHPGSVGRGFRDTEIRILDS
CCCHHHHHHHHHHCCCCCEEEEECCCCCCCEEEECCCHHHHCCCCCCCCCCCCEEEEEEC
QQRPLGPGEDGDVYLRAPMSAGYRYLGGAPPLPSTEDGFRSAGDIGHLDEDGYLYIVDRR
CCCCCCCCCCCCEEEECCHHCCCEECCCCCCCCCCCCHHHCCCCCCCCCCCCEEEEEECC
VDMIISGGANVFPAEVESALAGHPAIADVVVIGLADPQWGRRVHAVVQRADGASLTEQQV
EEEEEECCCCCCHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHH
IDYAKGRLAPYKAPKTVEFVDAIPRTAATKVNRSAMIAARGG
HHHHHCCCCCCCCCCCHHHHHHCCHHHHHHCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0013 {undecanoate}} 0.0022 {pentadecanoate}} 0.053 {CoA}} 0.035 {CoA}} 0.034 {CoA}} 0.0034 {tridecanoate}} 0.002 {laurate}} 0.0019 {laurate}} 0.0

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2254270; 1551828 [H]