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The map label for this gene is pykA

Identifier: 41407408

GI number: 41407408

Start: 1397456

End: 1398874

Strand: Direct

Name: pykA

Synonym: MAP1310

Alternate gene names: 41407408

Gene position: 1397456-1398874 (Clockwise)

Preceding gene: 41407407

Following gene: 41407409

Centisome position: 28.93

GC content: 68.29

Gene sequence:

>1419_bases
GTGAGTAGACGCGGGAAGATCGTCTGCACCCTTGGCCCGGCGACCAATTCGGACGAGTTGATTTTGGCGCTGGTCGAGGC
CGGAATGGACGTCGCCCGACTGAACTTCAGCCACGGCGACTACGCCGACCACAAAGCCGCCTACGAACGGGTGCGGGTGG
CCTCCGACGCCACCGGCCGCGCCGTCGGCGTGCTCGCCGATCTGCAGGGCCCCAAGATCCGCCTGGGGCGCTTTGCCACC
GGGCCCACCTACTGGGCCGACGGCGAAACGGTCCGCATCACCGTCGCCGACTGCGAAGGCAGCCACGACCGGGTGTCGAC
CACCTACAAACAACTGGCCCAGGACGCCGCGGTCGGGGACCGGGTCCTGGTCGACGACGGCAAGGTCGGCCTGGTGGTCG
ACGGCATCGAGGGCGACGACGTCATCTGCACCGTGGTCGAGGGCGGCCCGGTCAGCAACAACAAGGGCATCTCGCTGCCG
GGCATGAACGTCTCCGCACCGGCCTTGTCCGACAAGGACATCGAGGACCTCACCTTCGCGCTCGAGCTGGGCGTCGACCT
GGTCGCGCTGTCCTTCGTGCGTTCGCCCTCCGACGTCGAGCTGGTGCACGAGGTGATGGACCGCGTCGGCCGGCGGGTGC
CCGTGATCGCCAAACTGGAAAAGCCGGAGGCCGTCGACAACCTGGAAGCCATCGTGCTGGCCTTCGACGCCATCATGGTG
GCCCGCGGCGACCTCGGTGTCGAACTTCCCCTGGAAGAGGTTCCGCTGGTGCAGAAGCGGGCCATCCAGATGGCCCGGGA
GAACGCCAAACCCGTCATCGTGGCAACCCAGATGCTCGATTCGATGATCGAGAACTCACGGCCGACCCGGGCCGAGGCCT
CCGACGTCGCCAACGCCGTGCTGGACGGCGCCGACGCCGTGATGCTGTCCGGGGAGACCTCGGTGGGCAAGTACCCGCTG
GCGGCCGTGCGGACCATGGCGCGCATCATCTGCGCGGTCGAGGACAACTCCACGGCCGCCCCGCCGCTCACCCACGTGCC
CCGCACCAAACGCGGGGTGATCTCGTATGCGGCCCGCGACATCGGCGAACGCCTGGACGCCAAGGCGCTGGTCGCGTTCA
CCCAGTCCGGCGACACCGTCAAGCGGCTGGCGCGGTTGCACACCCCGCTGCCGCTGCTGGCCTTCACGGCATGGCCGGAG
GTGCGCAGCCAGCTGGCCATGACCTGGGGCACCGAGACGTTCATCGTGCCGATGATGACCTCCACCGACGGCATGATCCG
CCAGGTGGACAAGTCGCTGCTGGAACTCGGTCGGTACCGGCGTGGTGACCTGGTGGTGATCGTCGCCGGCGCGCCACCTG
GCACAGTAGGGTCAACCAACCTGATCCACGTGCACCGGATCGGGGAGGACGACGTCTAG

Upstream 100 bases:

>100_bases
CTTCCCCCTGTTTCCGACTGTTCTCGGTGGGTAACAGCACACTGCGCGCCGTGCGTTATGTGCGGGCCGGGACGCGGGCA
CACGACTATGCTGGGTCGGC

Downstream 100 bases:

>100_bases
CCTGCCCGGCGACGACACGGGCCGCTGCGCGGCCTGAGGAACGGGCGGGCGCATCGAACCAGAGGAGAGCTCGTGCCAGC
CGGCGCTGAATCCCAGGAGC

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MSRRGKIVCTLGPATNSDELILALVEAGMDVARLNFSHGDYADHKAAYERVRVASDATGRAVGVLADLQGPKIRLGRFAT
GPTYWADGETVRITVADCEGSHDRVSTTYKQLAQDAAVGDRVLVDDGKVGLVVDGIEGDDVICTVVEGGPVSNNKGISLP
GMNVSAPALSDKDIEDLTFALELGVDLVALSFVRSPSDVELVHEVMDRVGRRVPVIAKLEKPEAVDNLEAIVLAFDAIMV
ARGDLGVELPLEEVPLVQKRAIQMARENAKPVIVATQMLDSMIENSRPTRAEASDVANAVLDGADAVMLSGETSVGKYPL
AAVRTMARIICAVEDNSTAAPPLTHVPRTKRGVISYAARDIGERLDAKALVAFTQSGDTVKRLARLHTPLPLLAFTAWPE
VRSQLAMTWGTETFIVPMMTSTDGMIRQVDKSLLELGRYRRGDLVVIVAGAPPGTVGSTNLIHVHRIGEDDV

Sequences:

>Translated_472_residues
MSRRGKIVCTLGPATNSDELILALVEAGMDVARLNFSHGDYADHKAAYERVRVASDATGRAVGVLADLQGPKIRLGRFAT
GPTYWADGETVRITVADCEGSHDRVSTTYKQLAQDAAVGDRVLVDDGKVGLVVDGIEGDDVICTVVEGGPVSNNKGISLP
GMNVSAPALSDKDIEDLTFALELGVDLVALSFVRSPSDVELVHEVMDRVGRRVPVIAKLEKPEAVDNLEAIVLAFDAIMV
ARGDLGVELPLEEVPLVQKRAIQMARENAKPVIVATQMLDSMIENSRPTRAEASDVANAVLDGADAVMLSGETSVGKYPL
AAVRTMARIICAVEDNSTAAPPLTHVPRTKRGVISYAARDIGERLDAKALVAFTQSGDTVKRLARLHTPLPLLAFTAWPE
VRSQLAMTWGTETFIVPMMTSTDGMIRQVDKSLLELGRYRRGDLVVIVAGAPPGTVGSTNLIHVHRIGEDDV
>Mature_471_residues
SRRGKIVCTLGPATNSDELILALVEAGMDVARLNFSHGDYADHKAAYERVRVASDATGRAVGVLADLQGPKIRLGRFATG
PTYWADGETVRITVADCEGSHDRVSTTYKQLAQDAAVGDRVLVDDGKVGLVVDGIEGDDVICTVVEGGPVSNNKGISLPG
MNVSAPALSDKDIEDLTFALELGVDLVALSFVRSPSDVELVHEVMDRVGRRVPVIAKLEKPEAVDNLEAIVLAFDAIMVA
RGDLGVELPLEEVPLVQKRAIQMARENAKPVIVATQMLDSMIENSRPTRAEASDVANAVLDGADAVMLSGETSVGKYPLA
AVRTMARIICAVEDNSTAAPPLTHVPRTKRGVISYAARDIGERLDAKALVAFTQSGDTVKRLARLHTPLPLLAFTAWPEV
RSQLAMTWGTETFIVPMMTSTDGMIRQVDKSLLELGRYRRGDLVVIVAGAPPGTVGSTNLIHVHRIGEDDV

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=491, Percent_Identity=36.6598778004073, Blast_Score=287, Evalue=1e-77,
Organism=Homo sapiens, GI33286420, Length=491, Percent_Identity=36.6598778004073, Blast_Score=287, Evalue=1e-77,
Organism=Homo sapiens, GI33286418, Length=494, Percent_Identity=36.8421052631579, Blast_Score=287, Evalue=2e-77,
Organism=Homo sapiens, GI32967597, Length=494, Percent_Identity=36.8421052631579, Blast_Score=271, Evalue=1e-72,
Organism=Homo sapiens, GI10835121, Length=494, Percent_Identity=36.8421052631579, Blast_Score=271, Evalue=1e-72,
Organism=Homo sapiens, GI310128732, Length=288, Percent_Identity=38.5416666666667, Blast_Score=190, Evalue=2e-48,
Organism=Homo sapiens, GI310128730, Length=288, Percent_Identity=38.5416666666667, Blast_Score=190, Evalue=2e-48,
Organism=Homo sapiens, GI310128736, Length=236, Percent_Identity=36.0169491525424, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI310128734, Length=236, Percent_Identity=36.0169491525424, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI310128738, Length=214, Percent_Identity=35.5140186915888, Blast_Score=131, Evalue=2e-30,
Organism=Escherichia coli, GI1787965, Length=470, Percent_Identity=42.5531914893617, Blast_Score=350, Evalue=1e-97,
Organism=Escherichia coli, GI1788160, Length=478, Percent_Identity=39.1213389121339, Blast_Score=285, Evalue=5e-78,
Organism=Caenorhabditis elegans, GI17544584, Length=488, Percent_Identity=35.2459016393443, Blast_Score=286, Evalue=2e-77,
Organism=Caenorhabditis elegans, GI17506829, Length=483, Percent_Identity=37.6811594202899, Blast_Score=276, Evalue=2e-74,
Organism=Caenorhabditis elegans, GI71984413, Length=483, Percent_Identity=37.6811594202899, Blast_Score=276, Evalue=2e-74,
Organism=Caenorhabditis elegans, GI71984406, Length=485, Percent_Identity=37.5257731958763, Blast_Score=275, Evalue=3e-74,
Organism=Caenorhabditis elegans, GI17506831, Length=483, Percent_Identity=37.6811594202899, Blast_Score=275, Evalue=4e-74,
Organism=Saccharomyces cerevisiae, GI6319279, Length=483, Percent_Identity=34.5755693581781, Blast_Score=265, Evalue=8e-72,
Organism=Saccharomyces cerevisiae, GI6324923, Length=490, Percent_Identity=34.2857142857143, Blast_Score=244, Evalue=2e-65,
Organism=Drosophila melanogaster, GI24648964, Length=490, Percent_Identity=36.530612244898, Blast_Score=280, Evalue=2e-75,
Organism=Drosophila melanogaster, GI28571814, Length=490, Percent_Identity=36.530612244898, Blast_Score=279, Evalue=3e-75,
Organism=Drosophila melanogaster, GI24648966, Length=461, Percent_Identity=34.9240780911063, Blast_Score=253, Evalue=3e-67,
Organism=Drosophila melanogaster, GI24581235, Length=486, Percent_Identity=27.7777777777778, Blast_Score=207, Evalue=1e-53,
Organism=Drosophila melanogaster, GI24646914, Length=283, Percent_Identity=34.982332155477, Blast_Score=148, Evalue=7e-36,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 50453; Mature: 50322

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRRGKIVCTLGPATNSDELILALVEAGMDVARLNFSHGDYADHKAAYERVRVASDATGR
CCCCCCEEEEECCCCCCCCEEHEEHHCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCC
AVGVLADLQGPKIRLGRFATGPTYWADGETVRITVADCEGSHDRVSTTYKQLAQDAAVGD
EEEEEEECCCCEEEECEEECCCCEECCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCC
RVLVDDGKVGLVVDGIEGDDVICTVVEGGPVSNNKGISLPGMNVSAPALSDKDIEDLTFA
EEEEECCCEEEEEECCCCCCEEEEEECCCCCCCCCCEECCCCCCCCCCCCCCCHHHHHHH
LELGVDLVALSFVRSPSDVELVHEVMDRVGRRVPVIAKLEKPEAVDNLEAIVLAFDAIMV
HHHCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHE
ARGDLGVELPLEEVPLVQKRAIQMARENAKPVIVATQMLDSMIENSRPTRAEASDVANAV
ECCCCCCCCCHHHCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCCCCHHHHHHHHH
LDGADAVMLSGETSVGKYPLAAVRTMARIICAVEDNSTAAPPLTHVPRTKRGVISYAARD
HCCCCEEEEECCCCCCCCCHHHHHHHHHHEEEECCCCCCCCCCCCCCCCHHHHHHHHHHH
IGERLDAKALVAFTQSGDTVKRLARLHTPLPLLAFTAWPEVRSQLAMTWGTETFIVPMMT
HHHHCCCEEEEEEECCCHHHHHHHHHCCCCCHHHEECCHHHHHHEEHCCCCCEEEEEEEE
STDGMIRQVDKSLLELGRYRRGDLVVIVAGAPPGTVGSTNLIHVHRIGEDDV
CCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCC
>Mature Secondary Structure 
SRRGKIVCTLGPATNSDELILALVEAGMDVARLNFSHGDYADHKAAYERVRVASDATGR
CCCCCEEEEECCCCCCCCEEHEEHHCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCC
AVGVLADLQGPKIRLGRFATGPTYWADGETVRITVADCEGSHDRVSTTYKQLAQDAAVGD
EEEEEEECCCCEEEECEEECCCCEECCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCC
RVLVDDGKVGLVVDGIEGDDVICTVVEGGPVSNNKGISLPGMNVSAPALSDKDIEDLTFA
EEEEECCCEEEEEECCCCCCEEEEEECCCCCCCCCCEECCCCCCCCCCCCCCCHHHHHHH
LELGVDLVALSFVRSPSDVELVHEVMDRVGRRVPVIAKLEKPEAVDNLEAIVLAFDAIMV
HHHCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHE
ARGDLGVELPLEEVPLVQKRAIQMARENAKPVIVATQMLDSMIENSRPTRAEASDVANAV
ECCCCCCCCCHHHCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCCCCHHHHHHHHH
LDGADAVMLSGETSVGKYPLAAVRTMARIICAVEDNSTAAPPLTHVPRTKRGVISYAARD
HCCCCEEEEECCCCCCCCCHHHHHHHHHHEEEECCCCCCCCCCCCCCCCHHHHHHHHHHH
IGERLDAKALVAFTQSGDTVKRLARLHTPLPLLAFTAWPEVRSQLAMTWGTETFIVPMMT
HHHHCCCEEEEEEECCCHHHHHHHHHCCCCCHHHEECCHHHHHHEEHCCCCCEEEEEEEE
STDGMIRQVDKSLLELGRYRRGDLVVIVAGAPPGTVGSTNLIHVHRIGEDDV
CCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA