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The map label for this gene is hisF

Identifier: 41407397

GI number: 41407397

Start: 1387568

End: 1388365

Strand: Direct

Name: hisF

Synonym: MAP1299

Alternate gene names: 41407397

Gene position: 1387568-1388365 (Clockwise)

Preceding gene: 41407396

Following gene: 41407398

Centisome position: 28.73

GC content: 71.05

Gene sequence:

>798_bases
ATGAGCCCGAACTCCACCGGCCTGGCGGTACGCGTGATCCCCTGCCTGGACGTCGACGACGGGCGGGTGGTCAAGGGCGT
CAACTTCGAAAACCTTAGGGACGCAGGCGATCCCGTCGAACTTGCGGCCGTCTACGACGCCGAGGGCGCTGACGAGCTCA
CCTTCCTCGACGTGACCGCGTCGTCGTCGGGCCGCGCCACCATGCTGGACGTGGTGCGCCGCACCGCCGAACAGGTCTTC
ATTCCGCTCACCGTCGGCGGCGGGGTGCGCACGGTGGCCGACGTCGACGTGCTGCTGCGGGCCGGAGCCGACAAGGTCTC
CGTCAACACCGCCGCGATCGCGCGCCCCGAGCTGCTCGAGGAGATGGCGCGGCAGTTCGGCTCCCAGTGCATCGTGTTGT
CCGTGGACGCCCGCACGGTGCCGCCGGGCGCGGTGCCCACCCCGTCGGGCTGGGAGGTCACCACCCACGGCGGGCGGCGT
GGCACCGGCATCGATGCCGTCGAATGGGCTTCCCGCGGAGCCGATTTGGGCGTGGGGGAGATCCTGCTGAACTCGATGGA
CGCCGACGGCACCAAGGCCGGGTTCGACCTGGAGATGCTGCAGGCGGTGCGGTCCGCGGTCACCGTGCCGGTGATCGCCA
GCGGCGGCGCCGGGGCGGCCGAACACTTCGCGCCCGCGATCGAGGCCGGGGCCGACGCGGTGTTGGCGGCCAGCGTTTTC
CACTTCCGGGAGCTGACCATCGGACAGGTCAAGGCCGCCATGGCCGAGGCGGGCATACCGGTGAGGATGGTGCGATGA

Upstream 100 bases:

>100_bases
TGGACCCCGGCCTCGGATTCGGCGCTGGCCGCCGGACCCGGCGCGCACGCCGAGATCCTGGACATCCTGCGCAATATCGG
CCGACCGGAGGACTACTGAG

Downstream 100 bases:

>100_bases
CGCTCGACCCGCAGATCGCCGCGCGGCTGAAGCGCAACGCCGACGGCCTGGTCACGGCGGTGGTGCAGGAGCGCGGCAGC
AAGGACGTGCTGATGGTCGC

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 265; Mature: 264

Protein sequence:

>265_residues
MSPNSTGLAVRVIPCLDVDDGRVVKGVNFENLRDAGDPVELAAVYDAEGADELTFLDVTASSSGRATMLDVVRRTAEQVF
IPLTVGGGVRTVADVDVLLRAGADKVSVNTAAIARPELLEEMARQFGSQCIVLSVDARTVPPGAVPTPSGWEVTTHGGRR
GTGIDAVEWASRGADLGVGEILLNSMDADGTKAGFDLEMLQAVRSAVTVPVIASGGAGAAEHFAPAIEAGADAVLAASVF
HFRELTIGQVKAAMAEAGIPVRMVR

Sequences:

>Translated_265_residues
MSPNSTGLAVRVIPCLDVDDGRVVKGVNFENLRDAGDPVELAAVYDAEGADELTFLDVTASSSGRATMLDVVRRTAEQVF
IPLTVGGGVRTVADVDVLLRAGADKVSVNTAAIARPELLEEMARQFGSQCIVLSVDARTVPPGAVPTPSGWEVTTHGGRR
GTGIDAVEWASRGADLGVGEILLNSMDADGTKAGFDLEMLQAVRSAVTVPVIASGGAGAAEHFAPAIEAGADAVLAASVF
HFRELTIGQVKAAMAEAGIPVRMVR
>Mature_264_residues
SPNSTGLAVRVIPCLDVDDGRVVKGVNFENLRDAGDPVELAAVYDAEGADELTFLDVTASSSGRATMLDVVRRTAEQVFI
PLTVGGGVRTVADVDVLLRAGADKVSVNTAAIARPELLEEMARQFGSQCIVLSVDARTVPPGAVPTPSGWEVTTHGGRRG
TGIDAVEWASRGADLGVGEILLNSMDADGTKAGFDLEMLQAVRSAVTVPVIASGGAGAAEHFAPAIEAGADAVLAASVFH
FRELTIGQVKAAMAEAGIPVRMVR

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=260, Percent_Identity=45.7692307692308, Blast_Score=212, Evalue=2e-56,
Organism=Escherichia coli, GI87082028, Length=250, Percent_Identity=26.4, Blast_Score=80, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6319725, Length=317, Percent_Identity=36.5930599369085, Blast_Score=184, Evalue=2e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.-

Molecular weight: Translated: 27362; Mature: 27231

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPNSTGLAVRVIPCLDVDDGRVVKGVNFENLRDAGDPVELAAVYDAEGADELTFLDVTA
CCCCCCCEEEEEEEEEECCCCCEEECCCHHHHHCCCCCEEEEEEECCCCCCCEEEEEEEC
SSSGRATMLDVVRRTAEQVFIPLTVGGGVRTVADVDVLLRAGADKVSVNTAAIARPELLE
CCCCCHHHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHCCCCEEEECHHHHCCHHHHH
EMARQFGSQCIVLSVDARTVPPGAVPTPSGWEVTTHGGRRGTGIDAVEWASRGADLGVGE
HHHHHHCCEEEEEEECCCCCCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHCCCCCCHHH
ILLNSMDADGTKAGFDLEMLQAVRSAVTVPVIASGGAGAAEHFAPAIEAGADAVLAASVF
HHHHCCCCCCCCCCCCHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHH
HFRELTIGQVKAAMAEAGIPVRMVR
HHHHHHHHHHHHHHHHCCCCEEECC
>Mature Secondary Structure 
SPNSTGLAVRVIPCLDVDDGRVVKGVNFENLRDAGDPVELAAVYDAEGADELTFLDVTA
CCCCCCEEEEEEEEEECCCCCEEECCCHHHHHCCCCCEEEEEEECCCCCCCEEEEEEEC
SSSGRATMLDVVRRTAEQVFIPLTVGGGVRTVADVDVLLRAGADKVSVNTAAIARPELLE
CCCCCHHHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHCCCCEEEECHHHHCCHHHHH
EMARQFGSQCIVLSVDARTVPPGAVPTPSGWEVTTHGGRRGTGIDAVEWASRGADLGVGE
HHHHHHCCEEEEEEECCCCCCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHCCCCCCHHH
ILLNSMDADGTKAGFDLEMLQAVRSAVTVPVIASGGAGAAEHFAPAIEAGADAVLAASVF
HHHHCCCCCCCCCCCCHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHH
HFRELTIGQVKAAMAEAGIPVRMVR
HHHHHHHHHHHHHHHHCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA