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The map label for this gene is hisH

Identifier: 41407394

GI number: 41407394

Start: 1385387

End: 1386007

Strand: Direct

Name: hisH

Synonym: MAP1296

Alternate gene names: 41407394

Gene position: 1385387-1386007 (Clockwise)

Preceding gene: 41407393

Following gene: 41407395

Centisome position: 28.68

GC content: 71.01

Gene sequence:

>621_bases
GTGACAAGTAAATCGGTTGTTGTCCTCGACTACGGCTCGGGCAACCTGCGGTCGGCGCAGCGCGCGCTGGAGCGGGTCGG
CGCCTCGGTGCAGGTGACCGCCGACGCCGACGCCGCCGCGGCCGCCGACGGACTGGTGGTGCCCGGCGTCGGCGCCTACG
AGGCATGCATGACCGGGCTGCGCAAGATCGGCGGGGACCGCATCATCGCCGAGCGGGTCGCCGCGGGGCGTCCGGTGCTG
GGCGTGTGCGTCGGGATGCAGATCCTGTTCGCCCGCGGCGTCGAATTCTCGGTGGAGACCTCCGGATGCGGGCAGTGGCC
GGGATCGGTGACACGCCTGCAGGCGCCGGTGATCCCGCACATGGGCTGGAACGTGGTCGAATCCGGGCCGGACAGCGTGC
TGTTCCGCGGCCTCGACGCTGACACCCGGTTCTACTTCGTGCACTCCTACGCCGCCCAACAGTGGGAGGGTTCGCCCGAG
GCGGTGCTGACCTGGTCGCGCCACGAGGGGCCGTTTCTGGCCGCGGTCGAGGACGGCCCGCTGTCGGCCACGCAGTTTCA
CCCGGAGAAGAGCGGGGATGCCGGCGCGGCCGTGCTGCGCAATTGGATTGAGCGCCTGTGA

Upstream 100 bases:

>100_bases
ACATCACCGAAGCGCAGTACAAGGCGGTGGCCCGTGCGCTGCGTCAGGCCGTCGAGCCCGACCCGCGGGTGTCGGACGTG
CCGTCCACCAAAGGTGTTCT

Downstream 100 bases:

>100_bases
AAAGCAGCTCAGGAGGGAATGTGTTGATCTTGTTGCCCGCGGTCGACGTGGTCGACGGCCGCGCCGTGCGTTTGGTGCAG
GGCAAGGCGGGGAGTGAAAC

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH

Number of amino acids: Translated: 206; Mature: 205

Protein sequence:

>206_residues
MTSKSVVVLDYGSGNLRSAQRALERVGASVQVTADADAAAAADGLVVPGVGAYEACMTGLRKIGGDRIIAERVAAGRPVL
GVCVGMQILFARGVEFSVETSGCGQWPGSVTRLQAPVIPHMGWNVVESGPDSVLFRGLDADTRFYFVHSYAAQQWEGSPE
AVLTWSRHEGPFLAAVEDGPLSATQFHPEKSGDAGAAVLRNWIERL

Sequences:

>Translated_206_residues
MTSKSVVVLDYGSGNLRSAQRALERVGASVQVTADADAAAAADGLVVPGVGAYEACMTGLRKIGGDRIIAERVAAGRPVL
GVCVGMQILFARGVEFSVETSGCGQWPGSVTRLQAPVIPHMGWNVVESGPDSVLFRGLDADTRFYFVHSYAAQQWEGSPE
AVLTWSRHEGPFLAAVEDGPLSATQFHPEKSGDAGAAVLRNWIERL
>Mature_205_residues
TSKSVVVLDYGSGNLRSAQRALERVGASVQVTADADAAAAADGLVVPGVGAYEACMTGLRKIGGDRIIAERVAAGRPVLG
VCVGMQILFARGVEFSVETSGCGQWPGSVTRLQAPVIPHMGWNVVESGPDSVLFRGLDADTRFYFVHSYAAQQWEGSPEA
VLTWSRHEGPFLAAVEDGPLSATQFHPEKSGDAGAAVLRNWIERL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Escherichia coli, GI1788334, Length=205, Percent_Identity=38.0487804878049, Blast_Score=121, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6319725, Length=212, Percent_Identity=36.3207547169811, Blast_Score=123, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS5_MYCPA (P60600)

Other databases:

- EMBL:   AE016958
- RefSeq:   NP_960230.1
- ProteinModelPortal:   P60600
- SMR:   P60600
- EnsemblBacteria:   EBMYCT00000039570
- GeneID:   2718400
- GenomeReviews:   AE016958_GR
- KEGG:   mpa:MAP1296
- NMPDR:   fig|262316.1.peg.1296
- GeneTree:   EBGT00050000016176
- HOGENOM:   HBG292341
- OMA:   SVRFAFE
- ProtClustDB:   PRK13146
- GO:   GO:0005737
- HAMAP:   MF_00278
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226
- PIRSF:   PIRSF000495
- TIGRFAMs:   TIGR01855

Pfam domain/function: PF00117 GATase

EC number: 2.4.2.-

Molecular weight: Translated: 21784; Mature: 21653

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 83-83 ACT_SITE 187-187 ACT_SITE 189-189

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSKSVVVLDYGSGNLRSAQRALERVGASVQVTADADAAAAADGLVVPGVGAYEACMTGL
CCCCCEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCHHHCCCCEEECCCCHHHHHHHHH
RKIGGDRIIAERVAAGRPVLGVCVGMQILFARGVEFSVETSGCGQWPGSVTRLQAPVIPH
HHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCEEEECCCCCC
MGWNVVESGPDSVLFRGLDADTRFYFVHSYAAQQWEGSPEAVLTWSRHEGPFLAAVEDGP
CCCCCCCCCCCCEEEECCCCCCEEEEEEEHHHHHCCCCCCEEEEEECCCCCEEEEECCCC
LSATQFHPEKSGDAGAAVLRNWIERL
CCCEEECCCCCCCHHHHHHHHHHHHC
>Mature Secondary Structure 
TSKSVVVLDYGSGNLRSAQRALERVGASVQVTADADAAAAADGLVVPGVGAYEACMTGL
CCCCEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCHHHCCCCEEECCCCHHHHHHHHH
RKIGGDRIIAERVAAGRPVLGVCVGMQILFARGVEFSVETSGCGQWPGSVTRLQAPVIPH
HHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCEEEECCCCCC
MGWNVVESGPDSVLFRGLDADTRFYFVHSYAAQQWEGSPEAVLTWSRHEGPFLAAVEDGP
CCCCCCCCCCCCEEEECCCCCCEEEEEEEHHHHHCCCCCCEEEEEECCCCCEEEEECCCC
LSATQFHPEKSGDAGAAVLRNWIERL
CCCEEECCCCCCCHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA