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The map label for this gene is zraR [H]

Identifier: 39997847

GI number: 39997847

Start: 3027724

End: 3029130

Strand: Reverse

Name: zraR [H]

Synonym: GSU2753

Alternate gene names: 39997847

Gene position: 3029130-3027724 (Counterclockwise)

Preceding gene: 39997849

Following gene: 39997845

Centisome position: 79.42

GC content: 66.88

Gene sequence:

>1407_bases
ATGACCGAGAATCGCTATCCCCCCTTCGGCATCCTTCTGGTTGACGACGAACCGGCCTGGCTCCGTTCCCTCTCCCTGAC
CCTGGAGTCGTCCGCCGGCATCACGAATACCATCCTCTGCCAGGACAGCAGGGACGTTCCGGCGCTGCTGGAAGGGGGCG
GCATCGGCCTGGTACTCCTGGATCTCACCATGCCCCACCTGTCGGGGGAGGAACTCCTCGCCACGATCAGCGAGAGACAC
CCCGAGGTGGCGGTCATCGTCATCAGCGGCCTGAACCAGGTGGAGACCGCGGTCCGCTGCATGAAGCTCGGGGCTTTCGA
CTACTTTGTCAAGACCGACGAGGAGGACCGGCTCGTGGGGGGAGTTCTCCGGGCGGTCAGGATGCTGGAGCTGCAGCGGG
AGAACCGGGAGATGTCGAGCCGGCTCGTCTCCGGCGGCCTGCGGCACCCGGAGGCCTTTGCCGGCATCGTCACCGCCGAC
CGGGGCATGCTTTCCCTGTTCGCCTACATCGAGGCAGTGGCCGTGAGCCCCCAGCCGCTCCTCATCACCGGCGAGAGCGG
GGTGGGCAAGGAACTCCTGGCCCGGGCCGCCCATACCCTGAGCGGCTGCAAAGGGCAGCTGGTGGCGGTGAACGTGGCCG
GGCTGGACGACACGGTCTTCGCCGACACTCTCTTCGGTCACGTGCGCGGCGCCTTCACCGGTGCCGAGACGGCGCGGCGC
GGCATGGTGGAGGAGGCGGCCAACGGTACCCTGTTCCTGGACGAGATCGGCGACCTGGGCATCCCCTCCCAGGTTAAACT
CCTGCGGCTCCTCCAGGAAGGGGAGTACTTTCCTCTGGGGAGCGACCAGCCCCGGCGGCTCCGGGCGCGGGTCATCGTCG
CCACCCACCGCAACCTGGCGGCCCGGGAGGCTGCCGGAGAGTTCCGGCGCGACCTCTACTACCGGCTCCGCACCCACCAG
GTCCATATGCCGCCCCTTCGGGAGCGGAAAGGGGACATCCCGCTCCTGCTGGACCATTTCCTGGCCGAGGCAGCCCGCAC
CCTGGGAAAGAAAAAACCCACGCCACCGCCGGAACTGGCCCAGCATCTGGCCACCTACAATTTCCCGGGTAACGTGCGGG
AGTTGAAGTCGATGGTTTTCGATGCGGTCAGCGTCCACCGGGACCGGATGCTCTCCATGGAAAGCTTCGTCAGGGCCATC
GGCGGCACGGAAGAACCCGCCGGTACTCCGCCGCCCACTTTGAACCCCTTCGCCGGGTTCGACAAGCTCCCCACCTTTAC
CGACGCCGCCGAGTTCCTCGTGGAAGAGGCCCTCAATCGTTCCGGCGGTAACCAGACCCTCGCCGCCCGACTCCTCGGCA
TCTCCCAGCCCGCCCTCTCCAAGCGGCTCAAGATGATGCGGAAATGA

Upstream 100 bases:

>100_bases
CGGGGACTCTTTTCTCCGCGAGCCCTCCGCCCCCCTCCAGGAACCGTATGCGGTGGGACGGAAGGAGAAAAACCACGTCA
ATGATGGAGTCCATTCACTT

Downstream 100 bases:

>100_bases
ACCCCCATAACCGGAGGTTATAGCGATAACCTTGGTTATGGTTGTTTTAACCTGCCGAAATAACTGATAGTACCTGCAGC
GGTTCATTGCCGGCATAACC

Product: sigma-54 dependent DNA-binding response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MTENRYPPFGILLVDDEPAWLRSLSLTLESSAGITNTILCQDSRDVPALLEGGGIGLVLLDLTMPHLSGEELLATISERH
PEVAVIVISGLNQVETAVRCMKLGAFDYFVKTDEEDRLVGGVLRAVRMLELQRENREMSSRLVSGGLRHPEAFAGIVTAD
RGMLSLFAYIEAVAVSPQPLLITGESGVGKELLARAAHTLSGCKGQLVAVNVAGLDDTVFADTLFGHVRGAFTGAETARR
GMVEEAANGTLFLDEIGDLGIPSQVKLLRLLQEGEYFPLGSDQPRRLRARVIVATHRNLAAREAAGEFRRDLYYRLRTHQ
VHMPPLRERKGDIPLLLDHFLAEAARTLGKKKPTPPPELAQHLATYNFPGNVRELKSMVFDAVSVHRDRMLSMESFVRAI
GGTEEPAGTPPPTLNPFAGFDKLPTFTDAAEFLVEEALNRSGGNQTLAARLLGISQPALSKRLKMMRK

Sequences:

>Translated_468_residues
MTENRYPPFGILLVDDEPAWLRSLSLTLESSAGITNTILCQDSRDVPALLEGGGIGLVLLDLTMPHLSGEELLATISERH
PEVAVIVISGLNQVETAVRCMKLGAFDYFVKTDEEDRLVGGVLRAVRMLELQRENREMSSRLVSGGLRHPEAFAGIVTAD
RGMLSLFAYIEAVAVSPQPLLITGESGVGKELLARAAHTLSGCKGQLVAVNVAGLDDTVFADTLFGHVRGAFTGAETARR
GMVEEAANGTLFLDEIGDLGIPSQVKLLRLLQEGEYFPLGSDQPRRLRARVIVATHRNLAAREAAGEFRRDLYYRLRTHQ
VHMPPLRERKGDIPLLLDHFLAEAARTLGKKKPTPPPELAQHLATYNFPGNVRELKSMVFDAVSVHRDRMLSMESFVRAI
GGTEEPAGTPPPTLNPFAGFDKLPTFTDAAEFLVEEALNRSGGNQTLAARLLGISQPALSKRLKMMRK
>Mature_467_residues
TENRYPPFGILLVDDEPAWLRSLSLTLESSAGITNTILCQDSRDVPALLEGGGIGLVLLDLTMPHLSGEELLATISERHP
EVAVIVISGLNQVETAVRCMKLGAFDYFVKTDEEDRLVGGVLRAVRMLELQRENREMSSRLVSGGLRHPEAFAGIVTADR
GMLSLFAYIEAVAVSPQPLLITGESGVGKELLARAAHTLSGCKGQLVAVNVAGLDDTVFADTLFGHVRGAFTGAETARRG
MVEEAANGTLFLDEIGDLGIPSQVKLLRLLQEGEYFPLGSDQPRRLRARVIVATHRNLAAREAAGEFRRDLYYRLRTHQV
HMPPLRERKGDIPLLLDHFLAEAARTLGKKKPTPPPELAQHLATYNFPGNVRELKSMVFDAVSVHRDRMLSMESFVRAIG
GTEEPAGTPPPTLNPFAGFDKLPTFTDAAEFLVEEALNRSGGNQTLAARLLGISQPALSKRLKMMRK

Specific function: Member of the two-component regulatory system zraS/zraR. When activated by zraS it acts in conjunction with sigma-54 to regulate the expression of zraP. Positively autoregulates the expression of the zraSR operon [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=466, Percent_Identity=33.2618025751073, Blast_Score=234, Evalue=7e-63,
Organism=Escherichia coli, GI1790299, Length=480, Percent_Identity=33.9583333333333, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI1790437, Length=462, Percent_Identity=35.9307359307359, Blast_Score=232, Evalue=4e-62,
Organism=Escherichia coli, GI1788905, Length=466, Percent_Identity=34.1201716738197, Blast_Score=209, Evalue=4e-55,
Organism=Escherichia coli, GI1789087, Length=338, Percent_Identity=37.2781065088757, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI87082117, Length=269, Percent_Identity=41.2639405204461, Blast_Score=189, Evalue=3e-49,
Organism=Escherichia coli, GI1789233, Length=385, Percent_Identity=33.7662337662338, Blast_Score=188, Evalue=6e-49,
Organism=Escherichia coli, GI87082152, Length=227, Percent_Identity=44.9339207048458, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI87081872, Length=315, Percent_Identity=34.9206349206349, Blast_Score=151, Evalue=1e-37,
Organism=Escherichia coli, GI1786524, Length=315, Percent_Identity=37.1428571428571, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1787583, Length=278, Percent_Identity=33.4532374100719, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1789828, Length=239, Percent_Identity=37.6569037656904, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI87081858, Length=331, Percent_Identity=30.2114803625378, Blast_Score=102, Evalue=5e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 51128; Mature: 50996

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50931 HTH_LYSR ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTENRYPPFGILLVDDEPAWLRSLSLTLESSAGITNTILCQDSRDVPALLEGGGIGLVLL
CCCCCCCCEEEEEECCCCHHHHHHHHEEECCCCCCEEEEECCCCCCCHHCCCCCEEEEEE
DLTMPHLSGEELLATISERHPEVAVIVISGLNQVETAVRCMKLGAFDYFVKTDEEDRLVG
EEECCCCCHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHCCHHEEEECCCCHHHHH
GVLRAVRMLELQRENREMSSRLVSGGLRHPEAFAGIVTADRGMLSLFAYIEAVAVSPQPL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCCE
LITGESGVGKELLARAAHTLSGCKGQLVAVNVAGLDDTVFADTLFGHVRGAFTGAETARR
EEECCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCHHHHHH
GMVEEAANGTLFLDEIGDLGIPSQVKLLRLLQEGEYFPLGSDQPRRLRARVIVATHRNLA
HHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHEEEEEEEEECCCHH
AREAAGEFRRDLYYRLRTHQVHMPPLRERKGDIPLLLDHFLAEAARTLGKKKPTPPPELA
HHHHHHHHHHHHHHHHHHCEECCCCHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHH
QHLATYNFPGNVRELKSMVFDAVSVHRDRMLSMESFVRAIGGTEEPAGTPPPTLNPFAGF
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
DKLPTFTDAAEFLVEEALNRSGGNQTLAARLLGISQPALSKRLKMMRK
CCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
TENRYPPFGILLVDDEPAWLRSLSLTLESSAGITNTILCQDSRDVPALLEGGGIGLVLL
CCCCCCCEEEEEECCCCHHHHHHHHEEECCCCCCEEEEECCCCCCCHHCCCCCEEEEEE
DLTMPHLSGEELLATISERHPEVAVIVISGLNQVETAVRCMKLGAFDYFVKTDEEDRLVG
EEECCCCCHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHCCHHEEEECCCCHHHHH
GVLRAVRMLELQRENREMSSRLVSGGLRHPEAFAGIVTADRGMLSLFAYIEAVAVSPQPL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCCE
LITGESGVGKELLARAAHTLSGCKGQLVAVNVAGLDDTVFADTLFGHVRGAFTGAETARR
EEECCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCHHHHHH
GMVEEAANGTLFLDEIGDLGIPSQVKLLRLLQEGEYFPLGSDQPRRLRARVIVATHRNLA
HHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHEEEEEEEEECCCHH
AREAAGEFRRDLYYRLRTHQVHMPPLRERKGDIPLLLDHFLAEAARTLGKKKPTPPPELA
HHHHHHHHHHHHHHHHHHCEECCCCHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHH
QHLATYNFPGNVRELKSMVFDAVSVHRDRMLSMESFVRAIGGTEEPAGTPPPTLNPFAGF
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
DKLPTFTDAAEFLVEEALNRSGGNQTLAARLLGISQPALSKRLKMMRK
CCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]