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The map label for this gene is dcuB [H]

Identifier: 39997845

GI number: 39997845

Start: 3026124

End: 3027452

Strand: Reverse

Name: dcuB [H]

Synonym: GSU2751

Alternate gene names: 39997845

Gene position: 3027452-3026124 (Counterclockwise)

Preceding gene: 39997847

Following gene: 39997844

Centisome position: 79.37

GC content: 63.05

Gene sequence:

>1329_bases
ATGATGATGTTCTGGATCCAGTTCGTTCTCGTTCTCGGCGCGGTCCTGATCGGTATCCGCAGGGGTGGGGTGGCCCTGGG
CCTTATCGGAGGACTCGGCGTGTCGCTCCTGGTGCTCGGCTTCCGCAGCTCTCCGTCGGAGCCCCCCATCGCGGTCATGC
TCATCATCCTGGCGGTGGTCACCGCCTCGGCGACCCTGCAGGTGGCCGGGGGGCTCGACTACCTGGTGCAACTGACGGAG
AAGCTGTTGCGGGCCCACCCCAAGTACGTGACCATCCTGGCCCCGCTCTCCACCTTCTTCCTGACGGTCTGTGTCGGCAC
CGGCCACGCGGTCTATGCGCTGCTGCCGGTCATCGCCGACGTGGCCATCAAGACCGGTATCCGCCCCGAGCGGCCCATGG
CCATCTCCAGCGTTGCCTCCCAGATGGGGATCACCGCCAGTCCCGTGGCCGCGGCAGTGACCTTTTTCCTCGGCTTTGCC
TCCAAGTCCGGCTATCCCGTGACCCTCATCGACATAATCTCCGTCACCATGCCGGCCGGCGTGATCGGCGTGCTGGTGGC
CGCTGCCTGGAGCTTCAACCGGGGCAAGGACCTGGACAAGGACGCCGAGTACCAGGAGCGCCTCAAGGATGCCGAGTTCC
GCAAGGCCCTTGATGTCGATGTCACCACCCTGGACAAGAAGATCAGCACCACCGCCAAGGTGTCCGTGGCCCTCTTCTTT
GCCGGTGTGGCCACCATCATCCTCTTTGCGGTGTTTCCCGACCTGCTCCCCCTCAACGGCGAAAAGAAGCCGGTGCCCAT
GACCACGGTGGTCCAGTTCATCATGCTCGCCTACGGCGCCTTCATCATGTTCGCCGCCGACGTGAAGGCCAAGGACATCG
CCCACTCCAGCGTCTTTACCGCCGGCATGATCGCCGTGGTCTCCATCTTCGGCATCGCCTGGATGAGCGATACCTTCATT
TCCGCCAACAAGAAGTTCCTGGTGGACAACATCGGCGTCATGGTCAAGATGGCCCCCTGGACCTTTGCCATCGCCACCTT
CTGCATCTCCGCCTTTGTGAAGAGCCAGGCTGCCACCCTGGCCATCACGCTCCCGCTGGGCCTTGCCCTGGGGCTACCGG
TCCCGCTGCTGCTGGGGCTCATGCCGGCCAGCTACGCCTACTTCTTCTTTGCCTTCTATCCCAGCGACCTTGCAGCCATC
AACTTCGACCGTACCGGCACCACCCGCATCGGCAAATACCTCCTGAACCACAGCTTCATGATTCCCGGCCTGATCGGGGT
TAGCGTCTCCACGCTGGCGGCCTATGGGATTTCACAGTTCGTGCTGTAA

Upstream 100 bases:

>100_bases
CACGGTGTTGTCGTCCTTGGTATCCCCCTTGCTCATTGAAGGGTGAATCTGACAGACAACAGGTGCGGATCCAACGGGTC
CGACCTAAGGGGAGGCAGTT

Downstream 100 bases:

>100_bases
AAGAAGGGCAGCGACATGAGGCAGTCCGGGGGGGGTATCCCCCCTCGGGTATCAGGGGGAGCAGCAATGGAGGAGTCATG
ACGGGAGAGAGCGGTGGCAG

Product: anaerobic C4-dicarboxylate transporter

Products: C4-dicarboxylates [Periplasm]; C4-dicarboxylates [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MMMFWIQFVLVLGAVLIGIRRGGVALGLIGGLGVSLLVLGFRSSPSEPPIAVMLIILAVVTASATLQVAGGLDYLVQLTE
KLLRAHPKYVTILAPLSTFFLTVCVGTGHAVYALLPVIADVAIKTGIRPERPMAISSVASQMGITASPVAAAVTFFLGFA
SKSGYPVTLIDIISVTMPAGVIGVLVAAAWSFNRGKDLDKDAEYQERLKDAEFRKALDVDVTTLDKKISTTAKVSVALFF
AGVATIILFAVFPDLLPLNGEKKPVPMTTVVQFIMLAYGAFIMFAADVKAKDIAHSSVFTAGMIAVVSIFGIAWMSDTFI
SANKKFLVDNIGVMVKMAPWTFAIATFCISAFVKSQAATLAITLPLGLALGLPVPLLLGLMPASYAYFFFAFYPSDLAAI
NFDRTGTTRIGKYLLNHSFMIPGLIGVSVSTLAAYGISQFVL

Sequences:

>Translated_442_residues
MMMFWIQFVLVLGAVLIGIRRGGVALGLIGGLGVSLLVLGFRSSPSEPPIAVMLIILAVVTASATLQVAGGLDYLVQLTE
KLLRAHPKYVTILAPLSTFFLTVCVGTGHAVYALLPVIADVAIKTGIRPERPMAISSVASQMGITASPVAAAVTFFLGFA
SKSGYPVTLIDIISVTMPAGVIGVLVAAAWSFNRGKDLDKDAEYQERLKDAEFRKALDVDVTTLDKKISTTAKVSVALFF
AGVATIILFAVFPDLLPLNGEKKPVPMTTVVQFIMLAYGAFIMFAADVKAKDIAHSSVFTAGMIAVVSIFGIAWMSDTFI
SANKKFLVDNIGVMVKMAPWTFAIATFCISAFVKSQAATLAITLPLGLALGLPVPLLLGLMPASYAYFFFAFYPSDLAAI
NFDRTGTTRIGKYLLNHSFMIPGLIGVSVSTLAAYGISQFVL
>Mature_442_residues
MMMFWIQFVLVLGAVLIGIRRGGVALGLIGGLGVSLLVLGFRSSPSEPPIAVMLIILAVVTASATLQVAGGLDYLVQLTE
KLLRAHPKYVTILAPLSTFFLTVCVGTGHAVYALLPVIADVAIKTGIRPERPMAISSVASQMGITASPVAAAVTFFLGFA
SKSGYPVTLIDIISVTMPAGVIGVLVAAAWSFNRGKDLDKDAEYQERLKDAEFRKALDVDVTTLDKKISTTAKVSVALFF
AGVATIILFAVFPDLLPLNGEKKPVPMTTVVQFIMLAYGAFIMFAADVKAKDIAHSSVFTAGMIAVVSIFGIAWMSDTFI
SANKKFLVDNIGVMVKMAPWTFAIATFCISAFVKSQAATLAITLPLGLALGLPVPLLLGLMPASYAYFFFAFYPSDLAAI
NFDRTGTTRIGKYLLNHSFMIPGLIGVSVSTLAAYGISQFVL

Specific function: Responsible for the transport of C4-dicarboxylates from the periplasm across the inner membrane [H]

COG id: COG2704

COG function: function code R; Anaerobic C4-dicarboxylate transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dcuA/dcuB transporter (TC 2.A.13.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790565, Length=396, Percent_Identity=45.4545454545455, Blast_Score=342, Evalue=3e-95,
Organism=Escherichia coli, GI1790580, Length=447, Percent_Identity=38.0313199105145, Blast_Score=261, Evalue=7e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004668 [H]

Pfam domain/function: PF03605 DcuA_DcuB [H]

EC number: NA

Molecular weight: Translated: 46930; Mature: 46930

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMMFWIQFVLVLGAVLIGIRRGGVALGLIGGLGVSLLVLGFRSSPSEPPIAVMLIILAVV
CHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
TASATLQVAGGLDYLVQLTEKLLRAHPKYVTILAPLSTFFLTVCVGTGHAVYALLPVIAD
HCCCCEEECCCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHH
VAIKTGIRPERPMAISSVASQMGITASPVAAAVTFFLGFASKSGYPVTLIDIISVTMPAG
HHHHCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHH
VIGVLVAAAWSFNRGKDLDKDAEYQERLKDAEFRKALDVDVTTLDKKISTTAKVSVALFF
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
AGVATIILFAVFPDLLPLNGEKKPVPMTTVVQFIMLAYGAFIMFAADVKAKDIAHSSVFT
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
AGMIAVVSIFGIAWMSDTFISANKKFLVDNIGVMVKMAPWTFAIATFCISAFVKSQAATL
HHHHHHHHHHHHHHHCCHHHCCCCEEEEECCCEEEEECHHHHHHHHHHHHHHHHCCCCEE
AITLPLGLALGLPVPLLLGLMPASYAYFFFAFYPSDLAAINFDRTGTTRIGKYLLNHSFM
EEEEHHHHHHCCCHHHHHHHHHHHHHHHHHEECCCCEEEEECCCCCHHHHHHHHHCCCCC
IPGLIGVSVSTLAAYGISQFVL
CCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MMMFWIQFVLVLGAVLIGIRRGGVALGLIGGLGVSLLVLGFRSSPSEPPIAVMLIILAVV
CHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
TASATLQVAGGLDYLVQLTEKLLRAHPKYVTILAPLSTFFLTVCVGTGHAVYALLPVIAD
HCCCCEEECCCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHH
VAIKTGIRPERPMAISSVASQMGITASPVAAAVTFFLGFASKSGYPVTLIDIISVTMPAG
HHHHCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHH
VIGVLVAAAWSFNRGKDLDKDAEYQERLKDAEFRKALDVDVTTLDKKISTTAKVSVALFF
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
AGVATIILFAVFPDLLPLNGEKKPVPMTTVVQFIMLAYGAFIMFAADVKAKDIAHSSVFT
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
AGMIAVVSIFGIAWMSDTFISANKKFLVDNIGVMVKMAPWTFAIATFCISAFVKSQAATL
HHHHHHHHHHHHHHHCCHHHCCCCEEEEECCCEEEEECHHHHHHHHHHHHHHHHCCCCEE
AITLPLGLALGLPVPLLLGLMPASYAYFFFAFYPSDLAAINFDRTGTTRIGKYLLNHSFM
EEEEHHHHHHCCCHHHHHHHHHHHHHHHHHEECCCCEEEEECCCCCHHHHHHHHHCCCCC
IPGLIGVSVSTLAAYGISQFVL
CCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: C4-dicarboxylates [Periplasm]; C4-dicarboxylates [Periplasm] [C]

Specific reaction: C4-dicarboxylates [Periplasm] + C4-dicarboxylates [Periplasm] = C4-dicarboxylates [Periplasm] + C4-dicarboxylates [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]