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The map label for this gene is holB [H]

Identifier: 39997328

GI number: 39997328

Start: 2445628

End: 2446599

Strand: Direct

Name: holB [H]

Synonym: GSU2230

Alternate gene names: 39997328

Gene position: 2445628-2446599 (Clockwise)

Preceding gene: 39997327

Following gene: 39997329

Centisome position: 64.12

GC content: 66.26

Gene sequence:

>972_bases
ATGCCTTTTTCCCGGGTCCTCGGCCAGGAAACCGCCGTGAATGTCCTGCAACGCGCGCTCCGTTCGGGCAAAACGGCCCA
CGCCTACCTTTTCGAGGGGATCGAGGGGTGCGGCAAGATGACCACCGCCCTCGCCTTCATCGAGGCAGCCTTCTGCCGGC
GGGATGACGGCTGCGGTTCATGCCCCTCCTGCCGGAAAATGGCCGGACTCCAGCATCCGGACCTGCACCGACTGGCTCCC
GAGGGTGCATTCATCAAGATCGACCAGGTAAGGGAACTGCAGCGGGAGCTTTCCCTGCGTCCGGTTGAGGCACCGATGAA
GGCATGCATCATCGATGATGCCGACCGGCTCAACCCGGCAGCGGCCAACGCGCTGCTCAAGACGCTGGAAGAACCGCCGG
GCAATGCACTTCTGATCCTGCTCACCACGAATCCGGCGGGAATTCTCCCCACGGTCCGTTCCCGCTGTCAGCTCCTGAGG
TTTTCGCCGCTCCCCGAGCGGGTGATCGAAGAATACCTGACGGACGCCGGCCGGGAACCGGCCGCAGCCCGCCTGGCCGC
ATCCCTGGCCGGCGGCAGCCTTTCCCGGGCGCTGGAGATGGAGGAAGGGAGCGCCGGGACAGCCCGGCAGGGACTGATCG
AACGGCTTCGGACCCTCACCCTGGAAGAGATCACCCCCCTCTTCGCGGCCGCCGAGGAACTGGCCGCGGACCGGGAACGC
GCCGTGGAGGCCCTTGGCACCCTCACCTCCCTGTTGCGTGATGTTCTCCTCATCCAGGGGGGGAGTAACGAGGTGGTCAA
CCGGGATCTCGTCGACCTTCTCGAAGAAGAAGCGTGCCGGATGTCGCCGGAGCGGACCCTCACCCGCCTCGGCCACGTCA
TGGAGGCGCGCCAGGCGCTCCAGCGCAATGCAAATCCCCGGCTCACCATGGACGTGCTCCTCATGCGCCTGGCGGACCGG
ACGACACCGTAA

Upstream 100 bases:

>100_bases
CCCCACCGGTTCGCCGTGATCGACGGAAATGATGGAGTAGAAGCGACGGCGACGGCCGTGGCGGATGTACTCCTGAACCG
CGTGCCCCGGAGGTAGGACG

Downstream 100 bases:

>100_bases
CGCGCTTCGTTACACAACGGGCATTCAAACAAACAACGGGGAGCTCCCCGGGAGGATAGTGTGGTCAAAATCGTCAAGGT
CCAGTTTCATACCGCCGGGA

Product: DNA polymerase III subunit delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 323; Mature: 322

Protein sequence:

>323_residues
MPFSRVLGQETAVNVLQRALRSGKTAHAYLFEGIEGCGKMTTALAFIEAAFCRRDDGCGSCPSCRKMAGLQHPDLHRLAP
EGAFIKIDQVRELQRELSLRPVEAPMKACIIDDADRLNPAAANALLKTLEEPPGNALLILLTTNPAGILPTVRSRCQLLR
FSPLPERVIEEYLTDAGREPAAARLAASLAGGSLSRALEMEEGSAGTARQGLIERLRTLTLEEITPLFAAAEELAADRER
AVEALGTLTSLLRDVLLIQGGSNEVVNRDLVDLLEEEACRMSPERTLTRLGHVMEARQALQRNANPRLTMDVLLMRLADR
TTP

Sequences:

>Translated_323_residues
MPFSRVLGQETAVNVLQRALRSGKTAHAYLFEGIEGCGKMTTALAFIEAAFCRRDDGCGSCPSCRKMAGLQHPDLHRLAP
EGAFIKIDQVRELQRELSLRPVEAPMKACIIDDADRLNPAAANALLKTLEEPPGNALLILLTTNPAGILPTVRSRCQLLR
FSPLPERVIEEYLTDAGREPAAARLAASLAGGSLSRALEMEEGSAGTARQGLIERLRTLTLEEITPLFAAAEELAADRER
AVEALGTLTSLLRDVLLIQGGSNEVVNRDLVDLLEEEACRMSPERTLTRLGHVMEARQALQRNANPRLTMDVLLMRLADR
TTP
>Mature_322_residues
PFSRVLGQETAVNVLQRALRSGKTAHAYLFEGIEGCGKMTTALAFIEAAFCRRDDGCGSCPSCRKMAGLQHPDLHRLAPE
GAFIKIDQVRELQRELSLRPVEAPMKACIIDDADRLNPAAANALLKTLEEPPGNALLILLTTNPAGILPTVRSRCQLLRF
SPLPERVIEEYLTDAGREPAAARLAASLAGGSLSRALEMEEGSAGTARQGLIERLRTLTLEEITPLFAAAEELAADRERA
VEALGTLTSLLRDVLLIQGGSNEVVNRDLVDLLEEEACRMSPERTLTRLGHVMEARQALQRNANPRLTMDVLLMRLADRT
TP

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787341, Length=314, Percent_Identity=32.484076433121, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1786676, Length=250, Percent_Identity=30.4, Blast_Score=95, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004622 [H]

Pfam domain/function: NA

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 35222; Mature: 35091

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFSRVLGQETAVNVLQRALRSGKTAHAYLFEGIEGCGKMTTALAFIEAAFCRRDDGCGS
CCHHHHHCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
CPSCRKMAGLQHPDLHRLAPEGAFIKIDQVRELQRELSLRPVEAPMKACIIDDADRLNPA
CCHHHHHHCCCCCCHHHHCCCCCEEEHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHCCHH
AANALLKTLEEPPGNALLILLTTNPAGILPTVRSRCQLLRFSPLPERVIEEYLTDAGREP
HHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCH
AAARLAASLAGGSLSRALEMEEGSAGTARQGLIERLRTLTLEEITPLFAAAEELAADRER
HHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVEALGTLTSLLRDVLLIQGGSNEVVNRDLVDLLEEEACRMSPERTLTRLGHVMEARQAL
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
QRNANPRLTMDVLLMRLADRTTP
HHCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PFSRVLGQETAVNVLQRALRSGKTAHAYLFEGIEGCGKMTTALAFIEAAFCRRDDGCGS
CHHHHHCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
CPSCRKMAGLQHPDLHRLAPEGAFIKIDQVRELQRELSLRPVEAPMKACIIDDADRLNPA
CCHHHHHHCCCCCCHHHHCCCCCEEEHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHCCHH
AANALLKTLEEPPGNALLILLTTNPAGILPTVRSRCQLLRFSPLPERVIEEYLTDAGREP
HHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCH
AAARLAASLAGGSLSRALEMEEGSAGTARQGLIERLRTLTLEEITPLFAAAEELAADRER
HHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVEALGTLTSLLRDVLLIQGGSNEVVNRDLVDLLEEEACRMSPERTLTRLGHVMEARQAL
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
QRNANPRLTMDVLLMRLADRTTP
HHCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]