Click here to switch to the map view.

The map label for this gene is tmk

Identifier: 39997327

GI number: 39997327

Start: 2444982

End: 2445623

Strand: Direct

Name: tmk

Synonym: GSU2229

Alternate gene names: 39997327

Gene position: 2444982-2445623 (Clockwise)

Preceding gene: 39997304

Following gene: 39997328

Centisome position: 64.1

GC content: 67.13

Gene sequence:

>642_bases
ATGGGCTATTTCATTACATTCGAAGGCATCGAGGGGTGCGGCAAAACGACCCAGATCAAGCGGGCGGCGCAGTCCCTCAG
GGAGGCGGGCCACCGGGTGGTCGTTACGCGCGAACCGGGCGGTTGCCCCATTGCCGACGACATCCGGGCTATCCTGCTGG
ACGCCGGCAACAGCGCCATGGTACCCACGACGGAGCTCCTCCTCTACGCGGCGGCGCGTGCCCAGCACGTGGCCGAGGTC
ATCGCGCCCGCCCTGGCGGAGGGTGCCGTAGTCCTCTGCGATCGCTTTACCGATTCCACTCTCGTCTACCAGGGCTTCGG
CCGCAACCTGGACCGGGATCTCATTGCCCGGCTCAACACGCTGGCTGCGGGACAGATCCGGCCGGACCTCACAATCCTCC
TCGACTGCCCCGTGGCGGTGGGGCTTGCCCGGGCCGCGGCGCGGATCAACTCCCGGCAGACAAACCGGGAGGAACGTTTC
GAGCGGGAATCGCTCCTCTTTCACGAGCGGGTGCGGGAGGGATTCCTCTCCCTGGCCGGCGGGGAACCCCACCGGTTCGC
CGTGATCGACGGAAATGATGGAGTAGAAGCGACGGCGACGGCCGTGGCGGATGTACTCCTGAACCGCGTGCCCCGGAGGT
AG

Upstream 100 bases:

>100_bases
TCAATAGATATGCGGACCGACGGCCATTTTGCGCTTGATAATGCATGACCTCTCGCCTATAATTCAGCCTTTACGAATCG
AGAGGGTACATCCGGGCCGT

Downstream 100 bases:

>100_bases
GACGATGCCTTTTTCCCGGGTCCTCGGCCAGGAAACCGCCGTGAATGTCCTGCAACGCGCGCTCCGTTCGGGCAAAACGG
CCCACGCCTACCTTTTCGAG

Product: thymidylate kinase

Products: NA

Alternate protein names: dTMP kinase

Number of amino acids: Translated: 213; Mature: 212

Protein sequence:

>213_residues
MGYFITFEGIEGCGKTTQIKRAAQSLREAGHRVVVTREPGGCPIADDIRAILLDAGNSAMVPTTELLLYAAARAQHVAEV
IAPALAEGAVVLCDRFTDSTLVYQGFGRNLDRDLIARLNTLAAGQIRPDLTILLDCPVAVGLARAAARINSRQTNREERF
ERESLLFHERVREGFLSLAGGEPHRFAVIDGNDGVEATATAVADVLLNRVPRR

Sequences:

>Translated_213_residues
MGYFITFEGIEGCGKTTQIKRAAQSLREAGHRVVVTREPGGCPIADDIRAILLDAGNSAMVPTTELLLYAAARAQHVAEV
IAPALAEGAVVLCDRFTDSTLVYQGFGRNLDRDLIARLNTLAAGQIRPDLTILLDCPVAVGLARAAARINSRQTNREERF
ERESLLFHERVREGFLSLAGGEPHRFAVIDGNDGVEATATAVADVLLNRVPRR
>Mature_212_residues
GYFITFEGIEGCGKTTQIKRAAQSLREAGHRVVVTREPGGCPIADDIRAILLDAGNSAMVPTTELLLYAAARAQHVAEVI
APALAEGAVVLCDRFTDSTLVYQGFGRNLDRDLIARLNTLAAGQIRPDLTILLDCPVAVGLARAAARINSRQTNREERFE
RESLLFHERVREGFLSLAGGEPHRFAVIDGNDGVEATATAVADVLLNRVPRR

Specific function: Phosphorylation of dTMP to form dTDP in both de novo and salvage pathways of dTTP synthesis

COG id: COG0125

COG function: function code F; Thymidylate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate kinase family

Homologues:

Organism=Escherichia coli, GI1787340, Length=203, Percent_Identity=39.9014778325123, Blast_Score=130, Evalue=7e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KTHY_GEOSL (Q74AX0)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_953278.1
- ProteinModelPortal:   Q74AX0
- SMR:   Q74AX0
- GeneID:   2687571
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU2229
- NMPDR:   fig|243231.1.peg.2216
- TIGR:   GSU2229
- HOGENOM:   HBG626009
- OMA:   LFITLEG
- ProtClustDB:   CLSK924585
- BioCyc:   GSUL243231:GSU_2229-MONOMER
- BRENDA:   2.7.4.9
- HAMAP:   MF_00165
- InterPro:   IPR018095
- InterPro:   IPR018094
- PANTHER:   PTHR10344
- TIGRFAMs:   TIGR00041

Pfam domain/function: PF02223 Thymidylate_kin

EC number: =2.7.4.9

Molecular weight: Translated: 23062; Mature: 22930

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS01331 THYMIDYLATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGYFITFEGIEGCGKTTQIKRAAQSLREAGHRVVVTREPGGCPIADDIRAILLDAGNSAM
CCEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHEEEEECCCCCC
VPTTELLLYAAARAQHVAEVIAPALAEGAVVLCDRFTDSTLVYQGFGRNLDRDLIARLNT
CHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCEEEEECCCCCCCHHHHHHHHH
LAAGQIRPDLTILLDCPVAVGLARAAARINSRQTNREERFERESLLFHERVREGFLSLAG
HHCCCCCCCEEEEEECHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCC
GEPHRFAVIDGNDGVEATATAVADVLLNRVPRR
CCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
GYFITFEGIEGCGKTTQIKRAAQSLREAGHRVVVTREPGGCPIADDIRAILLDAGNSAM
CEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHEEEEECCCCCC
VPTTELLLYAAARAQHVAEVIAPALAEGAVVLCDRFTDSTLVYQGFGRNLDRDLIARLNT
CHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCEEEEECCCCCCCHHHHHHHHH
LAAGQIRPDLTILLDCPVAVGLARAAARINSRQTNREERFERESLLFHERVREGFLSLAG
HHCCCCCCCEEEEEECHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCC
GEPHRFAVIDGNDGVEATATAVADVLLNRVPRR
CCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA