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The map label for this gene is pfkA [H]

Identifier: 39997166

GI number: 39997166

Start: 2270294

End: 2271253

Strand: Direct

Name: pfkA [H]

Synonym: GSU2068

Alternate gene names: 39997166

Gene position: 2270294-2271253 (Clockwise)

Preceding gene: 39997165

Following gene: 39997175

Centisome position: 59.52

GC content: 62.4

Gene sequence:

>960_bases
ATGAAAAAAATCGGCATACTCACCAGCGGCGGCGACTGCTCGGGCATGAACGCCGCTATCCGGGCCGCGGTGCGCACGGC
TATCCGCATGAATATCGAAGTGGTCGGTTTCCGCAAGGGCTACCTTGGGCTCATGAAGGGGGATGCCATCCCCCTGGACA
CCAAGGCGGTCTCGGGCATTCTCCACCGGGGGGGCACCTTTCTCCAGTCGGCTCGCTCCCCCGAGTTCAAAACACCGGAA
GGACAGCGCACGGCCCTGAACAACCTGAAGGCCCTCGGCGTCGAGGGAATGGTTGTAATGGGCGGCGACGGGTCGCTCAC
CGGTGCCCTGGCCTTGAACCGTCTGGGATTGCCGGTGGTAGGCATTCCCGCCAGCATCGACAACGACATCCCGTTCACCG
ATATGGCACTGGGGGTTGACACGGCCCTCAACAACATCATCTACGCCGTTGACTGTATCAAGGACACGGCCAGCTCTCAC
GCGCGGGCCTTTGTCATCGAGGTTATGGGGCGCCATTCGGGCTACCTTGCCAGCATCTCCGCCATCGCCACCGGCGCCGA
GTACGCACTGGTCCCCGAACGGGAATACGATCTGGCCGAGATCTGTCAGCAGTTGCGGGCACGCTACGAGGAAGGGCGCG
ACAATGCCATCATCATTCTGGCGGAGGGGGCCGGTCATGGCCATGAAATCGCCAACAGCATCAAGGACGCCATCGGCTTC
GAGACGCGGGTGACGGTGCTGGGTCACTACCAGCGCGGAGGCGCGCCGACGGTATTTGACCGGCTGTTGGCGAGCCGCTT
GGGAAAGAAATCAGTTGAGCTGCTGGTGACAGGCACGTGGGGAGTGATGGTGGGACTGTCCTGCAATGCAATCCTCGCCA
CCCCCCTGGAGGATGTCATCAAGGGTGAGAAGCGTCCTCAGGACGAGGTGCTGCGCTTGGCCGAGGTGCTGGGCGTATGA

Upstream 100 bases:

>100_bases
TTGAAGGAAAACGATTTTATGCCCCGCGGGGGCACGGCTACGAGAAGACCATAAAAGAACGCATGGACTACATCCGCACA
CTGAAGGAGAACGACAGCGA

Downstream 100 bases:

>100_bases
GGGGGGGCGGTGCCGGGCGGCCCGGTCACCCGAGAAGGTCTATGATCTCGCGGTGGTGCTCCATCCGGTACGCGGCGGGG
AGCGGGGCCTTGTAGGCCAC

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase [H]

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MKKIGILTSGGDCSGMNAAIRAAVRTAIRMNIEVVGFRKGYLGLMKGDAIPLDTKAVSGILHRGGTFLQSARSPEFKTPE
GQRTALNNLKALGVEGMVVMGGDGSLTGALALNRLGLPVVGIPASIDNDIPFTDMALGVDTALNNIIYAVDCIKDTASSH
ARAFVIEVMGRHSGYLASISAIATGAEYALVPEREYDLAEICQQLRARYEEGRDNAIIILAEGAGHGHEIANSIKDAIGF
ETRVTVLGHYQRGGAPTVFDRLLASRLGKKSVELLVTGTWGVMVGLSCNAILATPLEDVIKGEKRPQDEVLRLAEVLGV

Sequences:

>Translated_319_residues
MKKIGILTSGGDCSGMNAAIRAAVRTAIRMNIEVVGFRKGYLGLMKGDAIPLDTKAVSGILHRGGTFLQSARSPEFKTPE
GQRTALNNLKALGVEGMVVMGGDGSLTGALALNRLGLPVVGIPASIDNDIPFTDMALGVDTALNNIIYAVDCIKDTASSH
ARAFVIEVMGRHSGYLASISAIATGAEYALVPEREYDLAEICQQLRARYEEGRDNAIIILAEGAGHGHEIANSIKDAIGF
ETRVTVLGHYQRGGAPTVFDRLLASRLGKKSVELLVTGTWGVMVGLSCNAILATPLEDVIKGEKRPQDEVLRLAEVLGV
>Mature_319_residues
MKKIGILTSGGDCSGMNAAIRAAVRTAIRMNIEVVGFRKGYLGLMKGDAIPLDTKAVSGILHRGGTFLQSARSPEFKTPE
GQRTALNNLKALGVEGMVVMGGDGSLTGALALNRLGLPVVGIPASIDNDIPFTDMALGVDTALNNIIYAVDCIKDTASSH
ARAFVIEVMGRHSGYLASISAIATGAEYALVPEREYDLAEICQQLRARYEEGRDNAIIILAEGAGHGHEIANSIKDAIGF
ETRVTVLGHYQRGGAPTVFDRLLASRLGKKSVELLVTGTWGVMVGLSCNAILATPLEDVIKGEKRPQDEVLRLAEVLGV

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI11321601, Length=356, Percent_Identity=41.8539325842697, Blast_Score=219, Evalue=2e-57,
Organism=Homo sapiens, GI48762920, Length=344, Percent_Identity=41.5697674418605, Blast_Score=218, Evalue=6e-57,
Organism=Homo sapiens, GI266453768, Length=358, Percent_Identity=41.0614525139665, Blast_Score=216, Evalue=2e-56,
Organism=Homo sapiens, GI266453748, Length=358, Percent_Identity=41.0614525139665, Blast_Score=216, Evalue=2e-56,
Organism=Homo sapiens, GI4505749, Length=358, Percent_Identity=41.0614525139665, Blast_Score=216, Evalue=2e-56,
Organism=Homo sapiens, GI266453619, Length=358, Percent_Identity=41.0614525139665, Blast_Score=216, Evalue=2e-56,
Organism=Escherichia coli, GI1790350, Length=306, Percent_Identity=45.4248366013072, Blast_Score=273, Evalue=1e-74,
Organism=Caenorhabditis elegans, GI25147584, Length=313, Percent_Identity=41.5335463258786, Blast_Score=212, Evalue=3e-55,
Organism=Caenorhabditis elegans, GI17558788, Length=308, Percent_Identity=37.3376623376623, Blast_Score=191, Evalue=5e-49,
Organism=Caenorhabditis elegans, GI72003601, Length=330, Percent_Identity=29.6969696969697, Blast_Score=144, Evalue=9e-35,
Organism=Saccharomyces cerevisiae, GI6321679, Length=341, Percent_Identity=41.6422287390029, Blast_Score=223, Evalue=3e-59,
Organism=Saccharomyces cerevisiae, GI6323861, Length=341, Percent_Identity=34.8973607038123, Blast_Score=183, Evalue=2e-47,
Organism=Drosophila melanogaster, GI28573326, Length=356, Percent_Identity=40.4494382022472, Blast_Score=225, Evalue=3e-59,
Organism=Drosophila melanogaster, GI24652337, Length=356, Percent_Identity=39.6067415730337, Blast_Score=219, Evalue=2e-57,
Organism=Drosophila melanogaster, GI17647809, Length=356, Percent_Identity=39.6067415730337, Blast_Score=219, Evalue=3e-57,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 33753; Mature: 33753

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIGILTSGGDCSGMNAAIRAAVRTAIRMNIEVVGFRKGYLGLMKGDAIPLDTKAVSGI
CCCEEEEECCCCCCCHHHHHHHHHHHHHHEEEEEEEECCCCEEEECCCCCCCCHHHHHHH
LHRGGTFLQSARSPEFKTPEGQRTALNNLKALGVEGMVVMGGDGSLTGALALNRLGLPVV
HHCCCHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCCCEE
GIPASIDNDIPFTDMALGVDTALNNIIYAVDCIKDTASSHARAFVIEVMGRHSGYLASIS
ECCCCCCCCCCCHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHEEHEEHHCCCCCHHEEHH
AIATGAEYALVPEREYDLAEICQQLRARYEEGRDNAIIILAEGAGHGHEIANSIKDAIGF
HHHCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHCC
ETRVTVLGHYQRGGAPTVFDRLLASRLGKKSVELLVTGTWGVMVGLSCNAILATPLEDVI
CEEEEEEEECCCCCCHHHHHHHHHHHCCCCCEEEEEEECHHEEEECCCCEEEECCHHHHH
KGEKRPQDEVLRLAEVLGV
CCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKKIGILTSGGDCSGMNAAIRAAVRTAIRMNIEVVGFRKGYLGLMKGDAIPLDTKAVSGI
CCCEEEEECCCCCCCHHHHHHHHHHHHHHEEEEEEEECCCCEEEECCCCCCCCHHHHHHH
LHRGGTFLQSARSPEFKTPEGQRTALNNLKALGVEGMVVMGGDGSLTGALALNRLGLPVV
HHCCCHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCCCEE
GIPASIDNDIPFTDMALGVDTALNNIIYAVDCIKDTASSHARAFVIEVMGRHSGYLASIS
ECCCCCCCCCCCHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHEEHEEHHCCCCCHHEEHH
AIATGAEYALVPEREYDLAEICQQLRARYEEGRDNAIIILAEGAGHGHEIANSIKDAIGF
HHHCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHCC
ETRVTVLGHYQRGGAPTVFDRLLASRLGKKSVELLVTGTWGVMVGLSCNAILATPLEDVI
CEEEEEEEECCCCCCHHHHHHHHHHHCCCCCEEEEEEECHHEEEECCCCEEEECCHHHHH
KGEKRPQDEVLRLAEVLGV
CCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA