Click here to switch to the map view.

The map label for this gene is yjeA [H]

Identifier: 39996852

GI number: 39996852

Start: 1915994

End: 1916917

Strand: Direct

Name: yjeA [H]

Synonym: GSU1753

Alternate gene names: 39996852

Gene position: 1915994-1916917 (Clockwise)

Preceding gene: 39996851

Following gene: 39996857

Centisome position: 50.23

GC content: 62.01

Gene sequence:

>924_bases
ATGACCGGTAACTGGAACCTGGCCCGCAGGCGCTCCGCGCTCTGGGCCAGGGCCCGCATCCTTGCAGAAATCAGACGTTT
TTTTGTTGAAGGGGGGTATCTCGAAGTCGAGACCCCCCTTCGCATTCCGGCACCTGCGCCCGAGTCCCACATCGATCCGG
TGCCGTCCGGTCCGTGGTTCCTTCAGACTTCGCCCGAACTGTGCATGAAACGGTTGGTGGCAGCAGGGTACGAGAGGGTT
TTCCAGATTTCGCGGTGTTGGCGCGATGGCGAACGGGGAAACATGCACCTGCCCGAGTTCACTATGCTCGAATGGTATCG
GGCCGGAACAGACTACCGTGGGCTCATGAATGAGTGCGAAGAACTCGTCGGCGCCGTGGCTGCAGCCCTCGGCATGGGGG
AAGTGCTGAACGTCAAGGGAAGAAACATCCGTCTGGGAGCCCCGTGGGAGCGTCTCTCCGTGGCAGAGGCCTTTGAACGC
TACTGCGACCGCACCATGGAGGAGGCCCTGGCCAACGACACCTTCGACGAGTGCATGGTTAACGACATTGAGCCGCGCCT
CGGCTTCGCGACCCCAACCTTCATCTGCGATTATCCCGCCGACCGCGGTGCCCTCGCCCGCCTGAAACAGGATGAGCCGT
CGCGCGCCGAACGCTTCGAGTTATACATCGCAGGCGTCGAGCTCGCCAACGCCTTTTCGGAGCTGACCGACCCCGTTGAG
CAGAGAAGCCGCTTCGAACAGGAGAAACTGTTCCGGGAGTCCCGCGGCAAAAGCGCCATTCCCCTGCCCGAGCCCTTTCT
CGCGGAACTCCACCACCTGCCGCCCACCGCCGGCATCGCCCTCGGCATTGACCGCATGGTGATGCTGCTCCTCGATCTTG
ATTCCATTGACGACGTAGTCGCCTTCACCCCGGAAGAACTGTAG

Upstream 100 bases:

>100_bases
GCTACATCCTCCGCGTTCCCCCGTTCATCGAGGAGGGCGAGATGATTGTCATCGATACCCGTTCCGGTGAATATTCAACA
CGGGTCAAAGGATAGACTCC

Downstream 100 bases:

>100_bases
CCGGCTGCCCCACATCACGGTATTCGATGGTTTCCCCCGTGTAGGTGCGTAACAGCCACAGGTCACCCCGACGGCTTATG
CAGTCCGGAAGCAACGGAAT

Product: lysyl-tRNA synthetase-related protein

Products: AMP; diphosphate; L-lysyl-tRNA(Lys)

Alternate protein names: NA

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MTGNWNLARRRSALWARARILAEIRRFFVEGGYLEVETPLRIPAPAPESHIDPVPSGPWFLQTSPELCMKRLVAAGYERV
FQISRCWRDGERGNMHLPEFTMLEWYRAGTDYRGLMNECEELVGAVAAALGMGEVLNVKGRNIRLGAPWERLSVAEAFER
YCDRTMEEALANDTFDECMVNDIEPRLGFATPTFICDYPADRGALARLKQDEPSRAERFELYIAGVELANAFSELTDPVE
QRSRFEQEKLFRESRGKSAIPLPEPFLAELHHLPPTAGIALGIDRMVMLLLDLDSIDDVVAFTPEEL

Sequences:

>Translated_307_residues
MTGNWNLARRRSALWARARILAEIRRFFVEGGYLEVETPLRIPAPAPESHIDPVPSGPWFLQTSPELCMKRLVAAGYERV
FQISRCWRDGERGNMHLPEFTMLEWYRAGTDYRGLMNECEELVGAVAAALGMGEVLNVKGRNIRLGAPWERLSVAEAFER
YCDRTMEEALANDTFDECMVNDIEPRLGFATPTFICDYPADRGALARLKQDEPSRAERFELYIAGVELANAFSELTDPVE
QRSRFEQEKLFRESRGKSAIPLPEPFLAELHHLPPTAGIALGIDRMVMLLLDLDSIDDVVAFTPEEL
>Mature_306_residues
TGNWNLARRRSALWARARILAEIRRFFVEGGYLEVETPLRIPAPAPESHIDPVPSGPWFLQTSPELCMKRLVAAGYERVF
QISRCWRDGERGNMHLPEFTMLEWYRAGTDYRGLMNECEELVGAVAAALGMGEVLNVKGRNIRLGAPWERLSVAEAFERY
CDRTMEEALANDTFDECMVNDIEPRLGFATPTFICDYPADRGALARLKQDEPSRAERFELYIAGVELANAFSELTDPVEQ
RSRFEQEKLFRESRGKSAIPLPEPFLAELHHLPPTAGIALGIDRMVMLLLDLDSIDDVVAFTPEEL

Specific function: Could Be A Lysyl-Trna Synthetase. Mutants In Poxa Have A Reduced Pyruvate Oxidase Activity And A Reduced Growth Rate. [C]

COG id: COG2269

COG function: function code J; Truncated, possibly inactive, lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI5031815, Length=330, Percent_Identity=31.2121212121212, Blast_Score=142, Evalue=4e-34,
Organism=Homo sapiens, GI194272210, Length=330, Percent_Identity=31.2121212121212, Blast_Score=142, Evalue=5e-34,
Organism=Escherichia coli, GI87082379, Length=336, Percent_Identity=37.5, Blast_Score=190, Evalue=1e-49,
Organism=Escherichia coli, GI1789256, Length=322, Percent_Identity=36.0248447204969, Blast_Score=172, Evalue=3e-44,
Organism=Escherichia coli, GI1790571, Length=324, Percent_Identity=34.8765432098765, Blast_Score=170, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI71994340, Length=335, Percent_Identity=29.5522388059701, Blast_Score=137, Evalue=8e-33,
Organism=Caenorhabditis elegans, GI17535925, Length=335, Percent_Identity=29.5522388059701, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17535927, Length=335, Percent_Identity=29.5522388059701, Blast_Score=136, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6320242, Length=334, Percent_Identity=28.7425149700599, Blast_Score=137, Evalue=3e-33,
Organism=Drosophila melanogaster, GI24640849, Length=340, Percent_Identity=32.6470588235294, Blast_Score=150, Evalue=8e-37,
Organism=Drosophila melanogaster, GI24640851, Length=340, Percent_Identity=32.6470588235294, Blast_Score=150, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR004525
- InterPro:   IPR018149 [H]

Pfam domain/function: PF00152 tRNA-synt_2 [H]

EC number: 6.1.1.6

Molecular weight: Translated: 34808; Mature: 34677

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGNWNLARRRSALWARARILAEIRRFFVEGGYLEVETPLRIPAPAPESHIDPVPSGPWF
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCEECCCCCCCCCCCCCCCCCEE
LQTSPELCMKRLVAAGYERVFQISRCWRDGERGNMHLPEFTMLEWYRAGTDYRGLMNECE
ECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHH
ELVGAVAAALGMGEVLNVKGRNIRLGAPWERLSVAEAFERYCDRTMEEALANDTFDECMV
HHHHHHHHHHCCCHHEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
NDIEPRLGFATPTFICDYPADRGALARLKQDEPSRAERFELYIAGVELANAFSELTDPVE
HCCCCCCCCCCCCEEECCCCCCHHHHHCCCCCCCHHHHEEHEEEHHHHHHHHHHHHHHHH
QRSRFEQEKLFRESRGKSAIPLPEPFLAELHHLPPTAGIALGIDRMVMLLLDLDSIDDVV
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHH
AFTPEEL
HCCCCCC
>Mature Secondary Structure 
TGNWNLARRRSALWARARILAEIRRFFVEGGYLEVETPLRIPAPAPESHIDPVPSGPWF
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCEECCCCCCCCCCCCCCCCCEE
LQTSPELCMKRLVAAGYERVFQISRCWRDGERGNMHLPEFTMLEWYRAGTDYRGLMNECE
ECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHH
ELVGAVAAALGMGEVLNVKGRNIRLGAPWERLSVAEAFERYCDRTMEEALANDTFDECMV
HHHHHHHHHHCCCHHEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
NDIEPRLGFATPTFICDYPADRGALARLKQDEPSRAERFELYIAGVELANAFSELTDPVE
HCCCCCCCCCCCCEEECCCCCCHHHHHCCCCCCCHHHHEEHEEEHHHHHHHHHHHHHHHH
QRSRFEQEKLFRESRGKSAIPLPEPFLAELHHLPPTAGIALGIDRMVMLLLDLDSIDDVV
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHH
AFTPEEL
HCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 2454 [C]

Specific activity: 1.79

Km value (mM): 0.0236 {L-Lys}} 0.0164 {L-Lys}} 0.0651 {ATP}} 0.0232 {ATP}} [C]

Substrates: ATP; L-lysine; tRNA(Lys)

Specific reaction: ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys)

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 6-Amino-n-hexanoic acid; Adenosine; ATP; Cadaverine; L-Lysineamide; L-Lysinehydroxamate; N-epsilon-Acetyl -L-lysine [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA