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The map label for this gene is yagI [H]

Identifier: 39995621

GI number: 39995621

Start: 548065

End: 548847

Strand: Reverse

Name: yagI [H]

Synonym: GSU0514

Alternate gene names: 39995621

Gene position: 548847-548065 (Counterclockwise)

Preceding gene: 39995623

Following gene: 39995617

Centisome position: 14.39

GC content: 56.7

Gene sequence:

>783_bases
ATGGCGAAGAAGGACAAATCCGAATACATCATCCAGGCAGTCGATCACGCCCTCGATCTCCTGGAACAGTTCCACGACGA
TGTGGACGAGCTGGGCGTGACCGAACTGTCAAAACGCCTGAAACTGCACAAGAACAATGTGTTCCGGCTCCTGGCAACCC
TGGAGTCGAGGGGATATATCGAGCAAAACAAGGTTACGGAGAATTACAGGCTCGGTCTCAAGACGCTTGAACTGGGCCAG
ACCTTCATCCGGCAGATGGGCCTGCTGCGCCAGTCGCGGCCAGTGCTTGAATGGCTCGTCAACCAGTGTAACGAAACCGC
CTACGTCGCCATCCTCAAGGATCAGAGCATTGTGTACCTGGACGTGGTGGAAACCGATCTGACGGTGCGGGTTGTTCCCC
GGGTCGGCTCTCGCCTCCCCGCTTACTGCACGGCGGCCGGCAAAGTCCAGATCGCCTGGATGAATGACGAGGAACTGGAC
AGCTTCTTCCCCCAGCGCGAACTGAAGCCTTTCACCCCCAATACCATCACTGACCGGGAAGTTCTCAGAAAACACCTCAG
GGAGGTGGTTGAGCAGGGGTATGCGGTGGACAACGAAGAACTCGATATCGGTGTGCGCTGCGTCGGCGCACCGATCCGTG
ATTACACCCGACGGATCATCGGCGCGGTGAGCCTTTCAGGACCTTCAATGCGCTTTACCGACGAGCGGCTGGACAAAGAG
TTGATACCGCTTGTCAAAAAGGCTGCCGACGAGATATCCATGAAGCTCGGCTTTCATCGGTAA

Upstream 100 bases:

>100_bases
TGTGAAGAGTCGACTGACGTGACCGGATTGATTGTCTTGACTTCCGTACGTTTCCGACTTAAATTGATACGTTCATCTTT
TCATGAGAAGAGGGATTGAG

Downstream 100 bases:

>100_bases
CAGAAAAGCCCCCGTCTACCGGGGGCTTTTTTCATGGGAAGCGCTTCGCCCAGATCTCCTCGATCCGTCGCTCCAACTCT
TCAGGTGTGCCGTTGTTGTC

Product: IclR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MAKKDKSEYIIQAVDHALDLLEQFHDDVDELGVTELSKRLKLHKNNVFRLLATLESRGYIEQNKVTENYRLGLKTLELGQ
TFIRQMGLLRQSRPVLEWLVNQCNETAYVAILKDQSIVYLDVVETDLTVRVVPRVGSRLPAYCTAAGKVQIAWMNDEELD
SFFPQRELKPFTPNTITDREVLRKHLREVVEQGYAVDNEELDIGVRCVGAPIRDYTRRIIGAVSLSGPSMRFTDERLDKE
LIPLVKKAADEISMKLGFHR

Sequences:

>Translated_260_residues
MAKKDKSEYIIQAVDHALDLLEQFHDDVDELGVTELSKRLKLHKNNVFRLLATLESRGYIEQNKVTENYRLGLKTLELGQ
TFIRQMGLLRQSRPVLEWLVNQCNETAYVAILKDQSIVYLDVVETDLTVRVVPRVGSRLPAYCTAAGKVQIAWMNDEELD
SFFPQRELKPFTPNTITDREVLRKHLREVVEQGYAVDNEELDIGVRCVGAPIRDYTRRIIGAVSLSGPSMRFTDERLDKE
LIPLVKKAADEISMKLGFHR
>Mature_259_residues
AKKDKSEYIIQAVDHALDLLEQFHDDVDELGVTELSKRLKLHKNNVFRLLATLESRGYIEQNKVTENYRLGLKTLELGQT
FIRQMGLLRQSRPVLEWLVNQCNETAYVAILKDQSIVYLDVVETDLTVRVVPRVGSRLPAYCTAAGKVQIAWMNDEELDS
FFPQRELKPFTPNTITDREVLRKHLREVVEQGYAVDNEELDIGVRCVGAPIRDYTRRIIGAVSLSGPSMRFTDERLDKEL
IPLVKKAADEISMKLGFHR

Specific function: Unknown

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI1786468, Length=249, Percent_Identity=36.144578313253, Blast_Score=167, Evalue=6e-43,
Organism=Escherichia coli, GI1788131, Length=230, Percent_Identity=37.3913043478261, Blast_Score=164, Evalue=7e-42,
Organism=Escherichia coli, GI87082359, Length=252, Percent_Identity=28.968253968254, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1786715, Length=247, Percent_Identity=31.5789473684211, Blast_Score=130, Evalue=9e-32,
Organism=Escherichia coli, GI1789999, Length=261, Percent_Identity=29.5019157088123, Blast_Score=115, Evalue=2e-27,
Organism=Escherichia coli, GI1790752, Length=226, Percent_Identity=32.3008849557522, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1788581, Length=248, Percent_Identity=25.8064516129032, Blast_Score=79, Evalue=3e-16,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 29844; Mature: 29713

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKDKSEYIIQAVDHALDLLEQFHDDVDELGVTELSKRLKLHKNNVFRLLATLESRGYI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
EQNKVTENYRLGLKTLELGQTFIRQMGLLRQSRPVLEWLVNQCNETAYVAILKDQSIVYL
CHHCCCHHHHHCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEEEECCCEEEE
DVVETDLTVRVVPRVGSRLPAYCTAAGKVQIAWMNDEELDSFFPQRELKPFTPNTITDRE
EEECCCCEEEEECHHCCCCCCCEECCCEEEEEECCCHHHHHHCCCCCCCCCCCCCCCHHH
VLRKHLREVVEQGYAVDNEELDIGVRCVGAPIRDYTRRIIGAVSLSGPSMRFTDERLDKE
HHHHHHHHHHHCCCCCCCCCCEECEEEECCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
LIPLVKKAADEISMKLGFHR
HHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
AKKDKSEYIIQAVDHALDLLEQFHDDVDELGVTELSKRLKLHKNNVFRLLATLESRGYI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
EQNKVTENYRLGLKTLELGQTFIRQMGLLRQSRPVLEWLVNQCNETAYVAILKDQSIVYL
CHHCCCHHHHHCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEEEECCCEEEE
DVVETDLTVRVVPRVGSRLPAYCTAAGKVQIAWMNDEELDSFFPQRELKPFTPNTITDRE
EEECCCCEEEEECHHCCCCCCCEECCCEEEEEECCCHHHHHHCCCCCCCCCCCCCCCHHH
VLRKHLREVVEQGYAVDNEELDIGVRCVGAPIRDYTRRIIGAVSLSGPSMRFTDERLDKE
HHHHHHHHHHHCCCCCCCCCCEECEEEECCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
LIPLVKKAADEISMKLGFHR
HHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]