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The map label for this gene is bcr [H]

Identifier: 39995374

GI number: 39995374

Start: 270803

End: 272038

Strand: Reverse

Name: bcr [H]

Synonym: GSU0264

Alternate gene names: 39995374

Gene position: 272038-270803 (Counterclockwise)

Preceding gene: 39995375

Following gene: 39995371

Centisome position: 7.13

GC content: 67.56

Gene sequence:

>1236_bases
ATGGAAATAACCTCCACGGCCCCGGCGTTCCAGCCGGCCGACATGACCCGGCGCGAACTGGCGCGACTGGTCCTCATCCT
GGGTGCGCTGACCGCTTTCAGCTCCATGTCCATCGACATGTACCTTCCCGCCTTCCCGCAGATCGCCCGGGACCTCGGCG
TGCCACTCGGAACGGTGCAGCTCTCCATCTCCGCCTTCCTGTTCGGCTCGGCCGCGGGGCAGCTCGTGTACGGTCCGCTG
GCCGACCGGTGGGGCAGGCGAACCCCTCTCCTCGGGGGACTGGCCCTCTATGTGGCGGCAGCCTTCGGATGCGCCGCCGT
CCACACGGGGGAGGGGCTGCTCTTCTGGCGGGTGGTTATGGCGCTGGGAGGCGGGGCCGGCATGGTGATCTCCCGCGCCG
TGGTGCGTGATCTCTACGACACCGCCGAGGCGGCGAGGATGTTTTCACTTCTCATGCTGGTCATGGGGGCCGCGCCGATC
CTGGCCCCAATCGCGGGGGGACAACTCCTGCTGATCACCGGCTGGCGGGGGATTTTCGGTTTTCTGGGGATCTTCGGCAT
TCTCTCTCTCGCCGCGGCAGCGGCATGCCTTCCCGAGTCGCTGCCGCCCGAACGGCGGAGCACGCGCGGTGTCGCCGAAA
TGGCCGCCATCTACGGCCATCTGCTCCGCAACCGCCGCTACCTCCGCTATGCCATCGCCCTGGGCTGCGTCGCCGGGGTC
AACTTCTCCTACATCTCAGGCTCCCCGTTCGTCTTCATCGAGCTGAACGGCGTCTCTCCCCAGCAGTTCGGCCTTTTCTT
CGGGGCCAACGCCTTCGGCCTGATCGGCGCATCCCAGGTGAACCGCCGGCTCCTGCGACGCTTCAGCGCCCAACGGATTC
TCAACGTCGCGTTCACCGTCAACGCAGCGGCGGCCCTGCTCCTCACGGTGGCGGGATTCACGGGGGTGGGCGGCTTCCCG
GCCCAGGTCGTCCTTCTGTTCGTGTGCCTCTGCATGACCGGATTTCTCTATCCCAACGTGACGGCCCTCGCCATGGCCCC
CTTCGACAAGGCGGCAGGGAGTGCATCGGCGCTTCTGGGAACGATCCAGTACACCCTGGGGGCAAGCGCGGGGGCTCTGG
TTGGGATGCTCCACAACGGAACTGCGGTGCCGATGACCGCTGCCATGGCGGCCTGCGGGCTGCTGGGCTGGTGTGCCGCC
GTGGCCGGAATCCCTCGCGCCCAAGTCGACCACTGA

Upstream 100 bases:

>100_bases
CTTCGGGTCGCTGCCGGCACTCTTCTGGCTGGTAAGCCTGTAAGAAACGGGAGCAATAGTGCGGCATGAACATCATTGGC
GGAACGGAGAACCCTGCCCT

Downstream 100 bases:

>100_bases
CCGCTTCCCCGGAGAACGTGCCCTGGCGCCATCAGGACTGGCTCAACAGCAACCACTGCCGGACCGGCCTCACACGCGGG
CGGAGAGACTCCCTCCGGGG

Product: Bcr/CflA subfamily drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Sulfonamide resistance protein [H]

Number of amino acids: Translated: 411; Mature: 411

Protein sequence:

>411_residues
MEITSTAPAFQPADMTRRELARLVLILGALTAFSSMSIDMYLPAFPQIARDLGVPLGTVQLSISAFLFGSAAGQLVYGPL
ADRWGRRTPLLGGLALYVAAAFGCAAVHTGEGLLFWRVVMALGGGAGMVISRAVVRDLYDTAEAARMFSLLMLVMGAAPI
LAPIAGGQLLLITGWRGIFGFLGIFGILSLAAAAACLPESLPPERRSTRGVAEMAAIYGHLLRNRRYLRYAIALGCVAGV
NFSYISGSPFVFIELNGVSPQQFGLFFGANAFGLIGASQVNRRLLRRFSAQRILNVAFTVNAAAALLLTVAGFTGVGGFP
AQVVLLFVCLCMTGFLYPNVTALAMAPFDKAAGSASALLGTIQYTLGASAGALVGMLHNGTAVPMTAAMAACGLLGWCAA
VAGIPRAQVDH

Sequences:

>Translated_411_residues
MEITSTAPAFQPADMTRRELARLVLILGALTAFSSMSIDMYLPAFPQIARDLGVPLGTVQLSISAFLFGSAAGQLVYGPL
ADRWGRRTPLLGGLALYVAAAFGCAAVHTGEGLLFWRVVMALGGGAGMVISRAVVRDLYDTAEAARMFSLLMLVMGAAPI
LAPIAGGQLLLITGWRGIFGFLGIFGILSLAAAAACLPESLPPERRSTRGVAEMAAIYGHLLRNRRYLRYAIALGCVAGV
NFSYISGSPFVFIELNGVSPQQFGLFFGANAFGLIGASQVNRRLLRRFSAQRILNVAFTVNAAAALLLTVAGFTGVGGFP
AQVVLLFVCLCMTGFLYPNVTALAMAPFDKAAGSASALLGTIQYTLGASAGALVGMLHNGTAVPMTAAMAACGLLGWCAA
VAGIPRAQVDH
>Mature_411_residues
MEITSTAPAFQPADMTRRELARLVLILGALTAFSSMSIDMYLPAFPQIARDLGVPLGTVQLSISAFLFGSAAGQLVYGPL
ADRWGRRTPLLGGLALYVAAAFGCAAVHTGEGLLFWRVVMALGGGAGMVISRAVVRDLYDTAEAARMFSLLMLVMGAAPI
LAPIAGGQLLLITGWRGIFGFLGIFGILSLAAAAACLPESLPPERRSTRGVAEMAAIYGHLLRNRRYLRYAIALGCVAGV
NFSYISGSPFVFIELNGVSPQQFGLFFGANAFGLIGASQVNRRLLRRFSAQRILNVAFTVNAAAALLLTVAGFTGVGGFP
AQVVLLFVCLCMTGFLYPNVTALAMAPFDKAAGSASALLGTIQYTLGASAGALVGMLHNGTAVPMTAAMAACGLLGWCAA
VAGIPRAQVDH

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=382, Percent_Identity=36.6492146596859, Blast_Score=215, Evalue=5e-57,
Organism=Escherichia coli, GI48994889, Length=356, Percent_Identity=31.4606741573034, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1790146, Length=347, Percent_Identity=27.0893371757925, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI87082312, Length=333, Percent_Identity=29.1291291291291, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1787065, Length=255, Percent_Identity=25.0980392156863, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI1790794, Length=237, Percent_Identity=22.3628691983122, Blast_Score=69, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6319657, Length=204, Percent_Identity=28.4313725490196, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42789; Mature: 42789

Theoretical pI: Translated: 9.62; Mature: 9.62

Prosite motif: PS50850 MFS ; PS00290 IG_MHC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEITSTAPAFQPADMTRRELARLVLILGALTAFSSMSIDMYLPAFPQIARDLGVPLGTVQ
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHCCCCHHHH
LSISAFLFGSAAGQLVYGPLADRWGRRTPLLGGLALYVAAAFGCAAVHTGEGLLFWRVVM
HHHHHHHHHCCCCCEEECCHHHHCCCCCCCHHHHHHHHHHHHHHHHEECCCCHHHHHHHH
ALGGGAGMVISRAVVRDLYDTAEAARMFSLLMLVMGAAPILAPIAGGQLLLITGWRGIFG
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEEECHHHHHH
FLGIFGILSLAAAAACLPESLPPERRSTRGVAEMAAIYGHLLRNRRYLRYAIALGCVAGV
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCC
NFSYISGSPFVFIELNGVSPQQFGLFFGANAFGLIGASQVNRRLLRRFSAQRILNVAFTV
CEEEECCCCEEEEEECCCCHHHHHHEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
NAAAALLLTVAGFTGVGGFPAQVVLLFVCLCMTGFLYPNVTALAMAPFDKAAGSASALLG
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHEEECCCHHCCCCHHHHHH
TIQYTLGASAGALVGMLHNGTAVPMTAAMAACGLLGWCAAVAGIPRAQVDH
HHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MEITSTAPAFQPADMTRRELARLVLILGALTAFSSMSIDMYLPAFPQIARDLGVPLGTVQ
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHCCCCHHHH
LSISAFLFGSAAGQLVYGPLADRWGRRTPLLGGLALYVAAAFGCAAVHTGEGLLFWRVVM
HHHHHHHHHCCCCCEEECCHHHHCCCCCCCHHHHHHHHHHHHHHHHEECCCCHHHHHHHH
ALGGGAGMVISRAVVRDLYDTAEAARMFSLLMLVMGAAPILAPIAGGQLLLITGWRGIFG
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEEECHHHHHH
FLGIFGILSLAAAAACLPESLPPERRSTRGVAEMAAIYGHLLRNRRYLRYAIALGCVAGV
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCC
NFSYISGSPFVFIELNGVSPQQFGLFFGANAFGLIGASQVNRRLLRRFSAQRILNVAFTV
CEEEECCCCEEEEEECCCCHHHHHHEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
NAAAALLLTVAGFTGVGGFPAQVVLLFVCLCMTGFLYPNVTALAMAPFDKAAGSASALLG
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHEEECCCHHCCCCHHHHHH
TIQYTLGASAGALVGMLHNGTAVPMTAAMAACGLLGWCAAVAGIPRAQVDH
HHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8486276; 9278503; 2694948 [H]