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The map label for this gene is hydA [H]

Identifier: 39995234

GI number: 39995234

Start: 137241

End: 138326

Strand: Reverse

Name: hydA [H]

Synonym: GSU0123

Alternate gene names: 39995234

Gene position: 138326-137241 (Counterclockwise)

Preceding gene: 39995235

Following gene: 39995233

Centisome position: 3.63

GC content: 66.21

Gene sequence:

>1086_bases
GTGAAACAGCATTGCAAGAGCTCGGGAACATGGGAAGAACAGGGGATTTCCCGGCGTGACTTCCTGAAGTTCTGCACCGC
AATGTCGGCGGTGCTGGCCCTGCCGGTTACCCTGGTGCCTCGAATCGCCGAGGCCCTGGAGGATGACAAGCGCCCCTCGG
TCATCTGGCTCGAATTCCAGAGCTGCACCGGCGATACCGAGGCACTCCTGAGAGCCGCCAATCCCACCGTGGCCGACATC
GTCCTGGACGTCCTCTCCGTGGACTACGCCGAGACCATCATGGCAGCGGCGGGCTTCCAGGCCGAAGCTGCCAGGCACGC
AACCATGAAAGCGCGATCCGGCACGTACATCGCCGTGGTGGAAGGAGCGATTCCGACCGGAGCAAACGGCGCCTACTGCT
GCATCGGCGGCCGGTCGGCCCTGGACATCACCCGCGAAGTCTGCGGCGGCGCCATGGCAACTATCACCGTCGGCACCTGC
GCCTCCTACGGGGGGATACCGGCGGCGGCGCCCAACCCGACCGGGGCGGTCGGCGTCAAGGATGCGGTGCCGGGGGCCAC
GGTCATCAACCTCCCCGGCTGCCCCGTCAACACCGACAACCTGGTGGCTACCGTGGTCCACCTGCTCACCTTCGGCTCCC
CGCCGGCCACCGACGGCAAGGGCCGCCCTCTCTTCGCCTACGGCAAGCGGATTCACGATAACTGCGAGCGACGGCCCCAC
TTTGACGCGGGCCAGTATGTGGAACAATGGGGCGATCAGGCACACCGGGCGGGCCACTGCCTCTACAAAATGGGCTGCAA
GGGCCCCGAAACCTTCCACAACTGCCCCTCCCAGCGCTACAACGAAAAGACAGCCTGGCCGGTTTCGGCGGGCCATGGCT
GCGCCGGCTGCTCCGAGCCCCACTTCTGGGATTCCATGACACCGCTCTACAAGCGGCTCCCCAATGTGCCCGGCTTCGGC
ATAGAGGCTACCGCCGACCAGATCGGCCTGGGAATCGCAGCGGCCACAGCGGCCGCATTCGGCATCCACGGCGTGGTGAG
CGCCCTGCGCAAGGGGAACGATTCCGACGATGAGAAGGAGGGGTAG

Upstream 100 bases:

>100_bases
TTTATCATACTGCCCTATCGTGAATATCCCCACCTGAGCCTTCCCACGCCTAATGAAGGAGGATGCTTTCATGGCAGGTA
ACGACGATGGCAACGACTTG

Downstream 100 bases:

>100_bases
CTCATGGCCATGATCGTTGTTGACCCGATCACGAGAATCGAGGGACATCTGCGCATCCAGGCCGAGGTGAACGGCGGACG
GATCACCGATGCCTGGAGCT

Product: nickel-dependent hydrogenase small subunit

Products: NA

Alternate protein names: Hydrogen:quinone oxidoreductase; Membrane-bound hydrogenase small subunit [H]

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MKQHCKSSGTWEEQGISRRDFLKFCTAMSAVLALPVTLVPRIAEALEDDKRPSVIWLEFQSCTGDTEALLRAANPTVADI
VLDVLSVDYAETIMAAAGFQAEAARHATMKARSGTYIAVVEGAIPTGANGAYCCIGGRSALDITREVCGGAMATITVGTC
ASYGGIPAAAPNPTGAVGVKDAVPGATVINLPGCPVNTDNLVATVVHLLTFGSPPATDGKGRPLFAYGKRIHDNCERRPH
FDAGQYVEQWGDQAHRAGHCLYKMGCKGPETFHNCPSQRYNEKTAWPVSAGHGCAGCSEPHFWDSMTPLYKRLPNVPGFG
IEATADQIGLGIAAATAAAFGIHGVVSALRKGNDSDDEKEG

Sequences:

>Translated_361_residues
MKQHCKSSGTWEEQGISRRDFLKFCTAMSAVLALPVTLVPRIAEALEDDKRPSVIWLEFQSCTGDTEALLRAANPTVADI
VLDVLSVDYAETIMAAAGFQAEAARHATMKARSGTYIAVVEGAIPTGANGAYCCIGGRSALDITREVCGGAMATITVGTC
ASYGGIPAAAPNPTGAVGVKDAVPGATVINLPGCPVNTDNLVATVVHLLTFGSPPATDGKGRPLFAYGKRIHDNCERRPH
FDAGQYVEQWGDQAHRAGHCLYKMGCKGPETFHNCPSQRYNEKTAWPVSAGHGCAGCSEPHFWDSMTPLYKRLPNVPGFG
IEATADQIGLGIAAATAAAFGIHGVVSALRKGNDSDDEKEG
>Mature_361_residues
MKQHCKSSGTWEEQGISRRDFLKFCTAMSAVLALPVTLVPRIAEALEDDKRPSVIWLEFQSCTGDTEALLRAANPTVADI
VLDVLSVDYAETIMAAAGFQAEAARHATMKARSGTYIAVVEGAIPTGANGAYCCIGGRSALDITREVCGGAMATITVGTC
ASYGGIPAAAPNPTGAVGVKDAVPGATVINLPGCPVNTDNLVATVVHLLTFGSPPATDGKGRPLFAYGKRIHDNCERRPH
FDAGQYVEQWGDQAHRAGHCLYKMGCKGPETFHNCPSQRYNEKTAWPVSAGHGCAGCSEPHFWDSMTPLYKRLPNVPGFG
IEATADQIGLGIAAATAAAFGIHGVVSALRKGNDSDDEKEG

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd2 Is Involved In Hydrogen Uptake. [C]

COG id: COG1740

COG function: function code C; Ni,Fe-hydrogenase I small subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family [H]

Homologues:

Organism=Escherichia coli, GI1789371, Length=287, Percent_Identity=48.0836236933798, Blast_Score=299, Evalue=2e-82,
Organism=Escherichia coli, GI1787206, Length=319, Percent_Identity=43.5736677115987, Blast_Score=290, Evalue=1e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR001821
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.12.5.1 [H]

Molecular weight: Translated: 37978; Mature: 37978

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
3.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQHCKSSGTWEEQGISRRDFLKFCTAMSAVLALPVTLVPRIAEALEDDKRPSVIWLEFQ
CCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEC
SCTGDTEALLRAANPTVADIVLDVLSVDYAETIMAAAGFQAEAARHATMKARSGTYIAVV
CCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEE
EGAIPTGANGAYCCIGGRSALDITREVCGGAMATITVGTCASYGGIPAAAPNPTGAVGVK
ECCCCCCCCCCEEEECCCHHHHHHHHHHCCEEEEEEHHHHHCCCCCCCCCCCCCCCCCCC
DAVPGATVINLPGCPVNTDNLVATVVHLLTFGSPPATDGKGRPLFAYGKRIHDNCERRPH
CCCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEHHHHHHHHHHHHCCCC
FDAGQYVEQWGDQAHRAGHCLYKMGCKGPETFHNCPSQRYNEKTAWPVSAGHGCAGCSEP
CCHHHHHHHHHHHHHHHCCHHHHHCCCCCHHHHCCCHHHCCCCCCCCCCCCCCCCCCCCC
HFWDSMTPLYKRLPNVPGFGIEATADQIGLGIAAATAAAFGIHGVVSALRKGNDSDDEKE
CCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
G
C
>Mature Secondary Structure
MKQHCKSSGTWEEQGISRRDFLKFCTAMSAVLALPVTLVPRIAEALEDDKRPSVIWLEFQ
CCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEC
SCTGDTEALLRAANPTVADIVLDVLSVDYAETIMAAAGFQAEAARHATMKARSGTYIAVV
CCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEE
EGAIPTGANGAYCCIGGRSALDITREVCGGAMATITVGTCASYGGIPAAAPNPTGAVGVK
ECCCCCCCCCCEEEECCCHHHHHHHHHHCCEEEEEEHHHHHCCCCCCCCCCCCCCCCCCC
DAVPGATVINLPGCPVNTDNLVATVVHLLTFGSPPATDGKGRPLFAYGKRIHDNCERRPH
CCCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEHHHHHHHHHHHHCCCC
FDAGQYVEQWGDQAHRAGHCLYKMGCKGPETFHNCPSQRYNEKTAWPVSAGHGCAGCSEP
CCHHHHHHHHHHHHHHHCCHHHHHCCCCCHHHHCCCHHHCCCCCCCCCCCCCCCCCCCCC
HFWDSMTPLYKRLPNVPGFGIEATADQIGLGIAAATAAAFGIHGVVSALRKGNDSDDEKE
CCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
G
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1587288; 8319698; 14500908 [H]