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The map label for this gene is otsB [H]

Identifier: 38234532

GI number: 38234532

Start: 2016061

End: 2016819

Strand: Direct

Name: otsB [H]

Synonym: DIP1968

Alternate gene names: 38234532

Gene position: 2016061-2016819 (Clockwise)

Preceding gene: 38234531

Following gene: 38234541

Centisome position: 81.01

GC content: 58.63

Gene sequence:

>759_bases
ATGTGGACTAGTAAGCAGCAGCAGGCGTTGGATCATCTGGCCGCCACCCCCTCGTTGTTGGTGGTGTCGGATTTTGATGG
CACTCTTGCGGGCTTTAGCCACGATGCCATGCAGGTGCCGGTTAATCATGTTGCAATGGCGGCGCTACAACAGCTCACGA
GCCTGCCGAGCACGCGAGTGGCATTGCTGTCGGGTCGCGATGTCCAAGGGCTGATTCAGGTTTCTGGTGCTGCCGCCCCG
ATGATTGTGGCGGGTTCGCATGGTGCAGAATCGACGGAAGGCGGGCTGGTTCTCAACGAGGATCAGCAGGCGGCGCTGAG
CGCGGTTACTGCGGCGCTCGAAGAGATTGCGACGTCAGTACCGGGGGCGTTTGTGGAGCATAAGCCCTATCATCGGGTGC
TTCATGTGATTCGAGTTGCCGATCAGCAGCGGGCACGTGCCGCGCTTGATCGCGCTCTTGCGCTAGAGATCCCTGGGGCG
AAGATTGCTGAGGGTAAGTGGATTGCAGAGGCCTCGGTGGTGGATTTTACAAAGGGCACGTGGATTAAGGCTGCGCAAGA
GCAGTATCGTCCTTCTGTAACACTGTTTTTGGGGGACGACACCACCGATGAAAACGGCTTTGCGGTGTTAGGCTGCAACG
ACGTAGGCATCAAGGTGGGTGCTGGTGATACCTGCGCCACGGTACGCCTTGAGTCCATTGACCGCGTGGGGCAGTTTTTG
TCTAGCCTCGCCGATGCTCGTTCGGCGGCACAACAGTAG

Upstream 100 bases:

>100_bases
GACTCGACTCTTGATATTCGCCATGGTTCCAGCCAGCAGATGGTGTTGACTCGCCAAGTGGATCAGGTGGATAAGCCGTC
GATTAGCTTGGTGGCTAACC

Downstream 100 bases:

>100_bases
CTCCTGGGTGGAAGGACGTTGCTAGGATCTCGCGGTGTAGTTGGCCACGGGAGATCACGTTAGGGTTCTTTTGTGCTTCT
TCGATCATCGAATAGAGCAA

Product: putative trehalose-phosphatase

Products: NA

Alternate protein names: TPP; Trehalose 6-phosphate phosphatase; Trehalose-phosphatase [H]

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MWTSKQQQALDHLAATPSLLVVSDFDGTLAGFSHDAMQVPVNHVAMAALQQLTSLPSTRVALLSGRDVQGLIQVSGAAAP
MIVAGSHGAESTEGGLVLNEDQQAALSAVTAALEEIATSVPGAFVEHKPYHRVLHVIRVADQQRARAALDRALALEIPGA
KIAEGKWIAEASVVDFTKGTWIKAAQEQYRPSVTLFLGDDTTDENGFAVLGCNDVGIKVGAGDTCATVRLESIDRVGQFL
SSLADARSAAQQ

Sequences:

>Translated_252_residues
MWTSKQQQALDHLAATPSLLVVSDFDGTLAGFSHDAMQVPVNHVAMAALQQLTSLPSTRVALLSGRDVQGLIQVSGAAAP
MIVAGSHGAESTEGGLVLNEDQQAALSAVTAALEEIATSVPGAFVEHKPYHRVLHVIRVADQQRARAALDRALALEIPGA
KIAEGKWIAEASVVDFTKGTWIKAAQEQYRPSVTLFLGDDTTDENGFAVLGCNDVGIKVGAGDTCATVRLESIDRVGQFL
SSLADARSAAQQ
>Mature_252_residues
MWTSKQQQALDHLAATPSLLVVSDFDGTLAGFSHDAMQVPVNHVAMAALQQLTSLPSTRVALLSGRDVQGLIQVSGAAAP
MIVAGSHGAESTEGGLVLNEDQQAALSAVTAALEEIATSVPGAFVEHKPYHRVLHVIRVADQQRARAALDRALALEIPGA
KIAEGKWIAEASVVDFTKGTWIKAAQEQYRPSVTLFLGDDTTDENGFAVLGCNDVGIKVGAGDTCATVRLESIDRVGQFL
SSLADARSAAQQ

Specific function: Removes the phosphate from trehalose 6-phosphate to produce free trehalose [H]

COG id: COG1877

COG function: function code G; Trehalose-6-phosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trehalose phosphatase family [H]

Homologues:

Organism=Escherichia coli, GI1788207, Length=230, Percent_Identity=29.1304347826087, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023214
- InterPro:   IPR006379
- InterPro:   IPR003337 [H]

Pfam domain/function: PF02358 Trehalose_PPase [H]

EC number: =3.1.3.12 [H]

Molecular weight: Translated: 26450; Mature: 26450

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWTSKQQQALDHLAATPSLLVVSDFDGTLAGFSHDAMQVPVNHVAMAALQQLTSLPSTRV
CCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHCCHHHHHHHHHHHHHCCCCCEE
ALLSGRDVQGLIQVSGAAAPMIVAGSHGAESTEGGLVLNEDQQAALSAVTAALEEIATSV
EEECCCCCCCCEEECCCCCCEEEECCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHC
PGAFVEHKPYHRVLHVIRVADQQRARAALDRALALEIPGAKIAEGKWIAEASVVDFTKGT
CCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEECCCCEEECCCEEECCCCC
WIKAAQEQYRPSVTLFLGDDTTDENGFAVLGCNDVGIKVGAGDTCATVRLESIDRVGQFL
HHHHHHHHCCCEEEEEECCCCCCCCCEEEEECCCCCEEECCCCCEEEEEHHHHHHHHHHH
SSLADARSAAQQ
HHHHHHHHHHCC
>Mature Secondary Structure
MWTSKQQQALDHLAATPSLLVVSDFDGTLAGFSHDAMQVPVNHVAMAALQQLTSLPSTRV
CCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHCCHHHHHHHHHHHHHCCCCCEE
ALLSGRDVQGLIQVSGAAAPMIVAGSHGAESTEGGLVLNEDQQAALSAVTAALEEIATSV
EEECCCCCCCCEEECCCCCCEEEECCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHC
PGAFVEHKPYHRVLHVIRVADQQRARAALDRALALEIPGAKIAEGKWIAEASVVDFTKGT
CCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEECCCCEEECCCEEECCCCC
WIKAAQEQYRPSVTLFLGDDTTDENGFAVLGCNDVGIKVGAGDTCATVRLESIDRVGQFL
HHHHHHHHCCCEEEEEECCCCCCCCCEEEEECCCCCEEECCCCCEEEEEHHHHHHHHHHH
SSLADARSAAQQ
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]