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The map label for this gene is nikA [H]

Identifier: 38234311

GI number: 38234311

Start: 1783772

End: 1785298

Strand: Direct

Name: nikA [H]

Synonym: DIP1740

Alternate gene names: 38234311

Gene position: 1783772-1785298 (Clockwise)

Preceding gene: 38234309

Following gene: 38234312

Centisome position: 71.68

GC content: 52.19

Gene sequence:

>1527_bases
ATGACTTCCTTCTCCCGGAGCGCGGTAGCAACATTGGCGCTAGCCGGCATTGTAGGGCTGAGCGGATGCTCGGCCGGATC
GACTTCATCTCAAGCACATCACATCTCCACTTCTGCCAAGGCTGTGTCCCTCGGACTAACGGCACCACCTGCTTCTCTCG
ACTTCACCACAGTGTCGGGCGCAGCAATCCCCCAAGCACTCATGGGCAACGTGTACGAAGGTTTGGTGCGAATCCAACAA
GATGGCACCATCGTCCCGTGGTTGGCACAGAGCTGGACCGTCTCGCCGGATGGCAAGGAGTATATTTTCCGGCTTCGCGA
CGGCGTGACGTTCTCCAATGGCGACGCTTTCACGGCAGAAACCGCAAAGTTTTCCATCGACGCAGTTAAATCCGACGCAT
GGACGAACGGATTGAAGAAACAAATGGCCAAGGTGGAAAGCACCGAAGCCATCGACCACCACACGTTAAAAGTGACGTTG
AAGGCTCGTTCCAATACGTGGCTGTGGTCCATGGGTACGCTCGTTGGCGCGATGATGACTCCCAACGGTGTGTCGGAGCT
AGCAACGAAACCAGTAGGCACTGGCCCCTACGTTGTAGATAAATGGGCAGTTGGCACTTCCCTATCGTTAGCTGCACGCC
CTGATTATTGGGGTGAACAAACACGCAATGACCGCGTAGTGTTCCGCTATTTTGGGGATGCCATCGCGCTAACAAATGCT
GTGCGTTCGGGTGATATTGATGCAGCGATTGGTCTGCAAAATCCGGAGCTTTTAGAGTCGATAACTTCTCGTGGTGACCT
AAAAGTTCATGTTGGTTCCACTAATGGCGAAGTGGTGCTCAGTATGAATAACAACCGTGCACCTTTTAATGACGTTCGCG
TGCGCCAGGCAGTCATGTATGGAGTAAACCGCCAAGACATTATTAACACCACGTGGGAAGGCTACGGAACTGACACCGGT
GGCCAGCCGGTTCCTCCTACAGATCCGTGGTACCAAGAAATGAATCGGTACCCATATGATCTAGAAAAGGCTCGCGAGCT
CATGCGCGAAGCTGGTGCGGTAGGTACTCCGATTACTATTTCGGTACCGTCTTTGCCCTACGCAAATTCTGCGTCGGAAA
TCGTGCTTTCTCAGCTACGCGATATCGGATTCGATGTTCGATTGGAATCCACGGAGTTTCCTTCCGTATGGTTGTCCAAG
GTTTATAAGGCCAAGGATTATGACATGTCCGTTGTGGCGCACGTTGAGGCCCGCGATATTCCGAATTTGTTTGGTAACCC
CGATTACTATTTGGGTTTTGATGATGAACAAGTTCGAGAGCTTTTAGCCCAGGCTGACTCCGGTGACGCAGACTCCTATG
TATCGACCATGAAGAAGGTAGTCAACCGAATTATGGATCAAGCTGGTGCAGATACCCTGTTTAACCTGCCTAATATCGTG
GTCACCCAACCTGATGTTTCTGGAGTTCCCACCAACTTGGTCGCTGATGGCCTCGTTGTGTCCAACATTTCCCGCTCGGA
GGACTAG

Upstream 100 bases:

>100_bases
ACATATACGTATCGGCTATCCCCCCGATTTAGGGATCAGCCTAAGATCACGTTCAGTGCAGTTTCAACCCCGAACATACC
ACCGGCTACCCTGTGTTTTT

Downstream 100 bases:

>100_bases
CCGATCATGCTTCTTACTCTTGCACGCGGTGCGGTGAAATACGTAACCACACTGATCGCCGCTAGCATTCTGATCTTTTT
TGCGCTTCGGATCGTGCCAG

Product: ABC transporter solute-binding protein

Products: Ni2; [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 508; Mature: 507

Protein sequence:

>508_residues
MTSFSRSAVATLALAGIVGLSGCSAGSTSSQAHHISTSAKAVSLGLTAPPASLDFTTVSGAAIPQALMGNVYEGLVRIQQ
DGTIVPWLAQSWTVSPDGKEYIFRLRDGVTFSNGDAFTAETAKFSIDAVKSDAWTNGLKKQMAKVESTEAIDHHTLKVTL
KARSNTWLWSMGTLVGAMMTPNGVSELATKPVGTGPYVVDKWAVGTSLSLAARPDYWGEQTRNDRVVFRYFGDAIALTNA
VRSGDIDAAIGLQNPELLESITSRGDLKVHVGSTNGEVVLSMNNNRAPFNDVRVRQAVMYGVNRQDIINTTWEGYGTDTG
GQPVPPTDPWYQEMNRYPYDLEKARELMREAGAVGTPITISVPSLPYANSASEIVLSQLRDIGFDVRLESTEFPSVWLSK
VYKAKDYDMSVVAHVEARDIPNLFGNPDYYLGFDDEQVRELLAQADSGDADSYVSTMKKVVNRIMDQAGADTLFNLPNIV
VTQPDVSGVPTNLVADGLVVSNISRSED

Sequences:

>Translated_508_residues
MTSFSRSAVATLALAGIVGLSGCSAGSTSSQAHHISTSAKAVSLGLTAPPASLDFTTVSGAAIPQALMGNVYEGLVRIQQ
DGTIVPWLAQSWTVSPDGKEYIFRLRDGVTFSNGDAFTAETAKFSIDAVKSDAWTNGLKKQMAKVESTEAIDHHTLKVTL
KARSNTWLWSMGTLVGAMMTPNGVSELATKPVGTGPYVVDKWAVGTSLSLAARPDYWGEQTRNDRVVFRYFGDAIALTNA
VRSGDIDAAIGLQNPELLESITSRGDLKVHVGSTNGEVVLSMNNNRAPFNDVRVRQAVMYGVNRQDIINTTWEGYGTDTG
GQPVPPTDPWYQEMNRYPYDLEKARELMREAGAVGTPITISVPSLPYANSASEIVLSQLRDIGFDVRLESTEFPSVWLSK
VYKAKDYDMSVVAHVEARDIPNLFGNPDYYLGFDDEQVRELLAQADSGDADSYVSTMKKVVNRIMDQAGADTLFNLPNIV
VTQPDVSGVPTNLVADGLVVSNISRSED
>Mature_507_residues
TSFSRSAVATLALAGIVGLSGCSAGSTSSQAHHISTSAKAVSLGLTAPPASLDFTTVSGAAIPQALMGNVYEGLVRIQQD
GTIVPWLAQSWTVSPDGKEYIFRLRDGVTFSNGDAFTAETAKFSIDAVKSDAWTNGLKKQMAKVESTEAIDHHTLKVTLK
ARSNTWLWSMGTLVGAMMTPNGVSELATKPVGTGPYVVDKWAVGTSLSLAARPDYWGEQTRNDRVVFRYFGDAIALTNAV
RSGDIDAAIGLQNPELLESITSRGDLKVHVGSTNGEVVLSMNNNRAPFNDVRVRQAVMYGVNRQDIINTTWEGYGTDTGG
QPVPPTDPWYQEMNRYPYDLEKARELMREAGAVGTPITISVPSLPYANSASEIVLSQLRDIGFDVRLESTEFPSVWLSKV
YKAKDYDMSVVAHVEARDIPNLFGNPDYYLGFDDEQVRELLAQADSGDADSYVSTMKKVVNRIMDQAGADTLFNLPNIVV
TQPDVSGVPTNLVADGLVVSNISRSED

Specific function: Involved in a nickel transport system, probably represents the nickel binder [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789887, Length=485, Percent_Identity=25.979381443299, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1787052, Length=312, Percent_Identity=30.1282051282051, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1789966, Length=396, Percent_Identity=25.5050505050505, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1787762, Length=268, Percent_Identity=27.2388059701493, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1787495, Length=387, Percent_Identity=27.906976744186, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1787551, Length=356, Percent_Identity=24.1573033707865, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI1789397, Length=285, Percent_Identity=27.719298245614, Blast_Score=82, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011980
- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 54881; Mature: 54750

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSFSRSAVATLALAGIVGLSGCSAGSTSSQAHHISTSAKAVSLGLTAPPASLDFTTVSG
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCEEEEECCCCCCCCEEEECCC
AAIPQALMGNVYEGLVRIQQDGTIVPWLAQSWTVSPDGKEYIFRLRDGVTFSNGDAFTAE
HHHHHHHHHHHHHHHEEECCCCCEEEEECCCEECCCCCHHHHEEHCCCCEECCCCEEEEC
TAKFSIDAVKSDAWTNGLKKQMAKVESTEAIDHHTLKVTLKARSNTWLWSMGTLVGAMMT
CCEEEEEHHHCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCEEEEHHHHHHHHCC
PNGVSELATKPVGTGPYVVDKWAVGTSLSLAARPDYWGEQTRNDRVVFRYFGDAIALTNA
CCCHHHHHCCCCCCCCEEEEEECCCCCEEEEECCCCCCCCCCCCCEEEEECCCHHHHHHH
VRSGDIDAAIGLQNPELLESITSRGDLKVHVGSTNGEVVLSMNNNRAPFNDVRVRQAVMY
HHCCCCCEEECCCCHHHHHHHHCCCCEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHH
GVNRQDIINTTWEGYGTDTGGQPVPPTDPWYQEMNRYPYDLEKARELMREAGAVGTPITI
CCCHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHCCCCCCCEEE
SVPSLPYANSASEIVLSQLRDIGFDVRLESTEFPSVWLSKVYKAKDYDMSVVAHVEARDI
ECCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHCCCCCEEEEEEECHHCC
PNLFGNPDYYLGFDDEQVRELLAQADSGDADSYVSTMKKVVNRIMDQAGADTLFNLPNIV
CHHCCCCCEEECCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEE
VTQPDVSGVPTNLVADGLVVSNISRSED
EECCCCCCCCHHHHHCCCCEECCCCCCC
>Mature Secondary Structure 
TSFSRSAVATLALAGIVGLSGCSAGSTSSQAHHISTSAKAVSLGLTAPPASLDFTTVSG
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCEEEEECCCCCCCCEEEECCC
AAIPQALMGNVYEGLVRIQQDGTIVPWLAQSWTVSPDGKEYIFRLRDGVTFSNGDAFTAE
HHHHHHHHHHHHHHHEEECCCCCEEEEECCCEECCCCCHHHHEEHCCCCEECCCCEEEEC
TAKFSIDAVKSDAWTNGLKKQMAKVESTEAIDHHTLKVTLKARSNTWLWSMGTLVGAMMT
CCEEEEEHHHCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCEEEEHHHHHHHHCC
PNGVSELATKPVGTGPYVVDKWAVGTSLSLAARPDYWGEQTRNDRVVFRYFGDAIALTNA
CCCHHHHHCCCCCCCCEEEEEECCCCCEEEEECCCCCCCCCCCCCEEEEECCCHHHHHHH
VRSGDIDAAIGLQNPELLESITSRGDLKVHVGSTNGEVVLSMNNNRAPFNDVRVRQAVMY
HHCCCCCEEECCCCHHHHHHHHCCCCEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHH
GVNRQDIINTTWEGYGTDTGGQPVPPTDPWYQEMNRYPYDLEKARELMREAGAVGTPITI
CCCHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHCCCCCCCEEE
SVPSLPYANSASEIVLSQLRDIGFDVRLESTEFPSVWLSKVYKAKDYDMSVVAHVEARDI
ECCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHCCCCCEEEEEEECHHCC
PNLFGNPDYYLGFDDEQVRELLAQADSGDADSYVSTMKKVVNRIMDQAGADTLFNLPNIV
CHHCCCCCEEECCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEE
VTQPDVSGVPTNLVADGLVVSNISRSED
EECCCCCCCCHHHHHCCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Ni2; [Periplasm]; ATP; H2O [C]

Specific reaction: Ni2+ [Periplasm] + ATP + H2O = Ni2+ [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7934931; 8041620; 9278503 [H]