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The map label for this gene is murF [H]

Identifier: 38234177

GI number: 38234177

Start: 1632828

End: 1634318

Strand: Reverse

Name: murF [H]

Synonym: DIP1602

Alternate gene names: 38234177

Gene position: 1634318-1632828 (Counterclockwise)

Preceding gene: 38234178

Following gene: 38234176

Centisome position: 65.67

GC content: 52.31

Gene sequence:

>1491_bases
ATGATTGATCTGACTGTTGAACAGATCGCACGTATCGTGGGTGGAACACTCACACAAGGCACTGATCCAGAAACCGTAGT
GACGGGACCTGTGGAATTTGACTCGCGCAAGGTATCGGAAGGTTCTTTGTTTGTGGCGTTGGCCGGAGCGCGTGTCGACG
GCCATGATTTCGTGCCAGCTGCGTTAGAAAGTGGCGCTGCAGCAGCATTGGTAGCGCATCCTGTGAAGATCGATTCTGGA
TTAGCGCCCGCTATTGTGGTGCCACCTGCGACTGGTCAACAGCAATCGGCCAACAGTTACGCAACTGAACATGATGAAGA
CGGTTCAGTTGCGGCTGTTCTCCAAGGTATGGGACTTATTGCGCGAAGAGTCATCGATACGTTGACTGCTCAGGGGTTAA
CCGTCATGGGTGTTACTGGTTCGGCAGGGAAAACCTCGACCAAAGACATGTTGGCGTCAATTCTGAGTAATCAGGCCCCT
ACAGTGGCCCCTCCTGGATCTTTCAATAACGAGATCGGGCATCCATATACAGCTTTACGCTGTACCGAACAGACCCGTTA
TCTAGTAGCGGAGATGTCTGCCCGCGGCATTGGCCACATAGCACATCTAGCACAGATTGCCCCTCCTAAAATCGGTGTTG
TGCTCAATGTTGGCACCGCGCATCTAGGCGAATTTGGTTCGCGCGAAGTTATTGCTCAAGCCAAAGGCGAATTAGTGGAG
GCACTTCCTCACGACGGTGTGGCTGTACTTAATGCAGATGATGATCTCGTTGCAGGCATGCGTACTCGAACTGATGCGAA
GGTGATCACTTTTTCCGCGAATTCGCAGGACGCGGATGTTTATGCCACCGATATTGAACTTGACGATTATGCGCGTGCTC
GTTTTACCCTCCACATTGGCGAAGCGGAGAACGTCGTAACGCTTCGAGTTGCTGGCCAGCATCAAGTTTCCAATGCACTG
GCAGCTACAGCGGCGGCATATGCGGCAGGCATAGCCATTGACGATATTGCCGAAGCATTGAGTAACCACCTTGGCGCTTC
TGCGCACCGTATGGCTCTTATGCACCGTAGCGATGGCGCAACCATCATTGATGATGCTTATAACGCCAACACTGAGTCGA
TGCGTGCCGGTCTTACGGCGCTTTCCACTACTGCTCACAACAAGTCGCACAAGGGCGAAGCCTCGCACGCGTGGGCAGTG
TTGGGTCCCATGAGTGAGCTAGGGGAGGAGTCGGTTGCGGCACACGAAGAGATTGGCAGGCTCCTCGGTGAGTTGAATGT
GCATGGATTAATCGTTGTCGGAGACTCTGCTGATTCTTTGGCATTGGCAGATGGAGCCCGACGCAGCAATGTGACTACAC
TTATGGCTCGCGATACCGCAGAAGCAGCAGATCTCCTTGATCAGAATATGGGTTCTGCAGACGTGGCTCTTGTAAAAGCA
TCTAATGCATATCGTTTGTGGGAAACCGCAGAGATTTTAGCTTCAAAATAA

Upstream 100 bases:

>100_bases
AGGAAGTAGCAGCTGCTCTGCATGATCAAGGGTTCGACCTCGATTAATGGATGAATAGCCGGTTTTCATTGCATTTCATT
GTTGTTAAGGACTTGGGAAA

Downstream 100 bases:

>100_bases
AGAAAGAATGACAGGTGAATCAACAATGACCCAGATCATTATTGCGGGCGCTGTGGGCCTTTTGGTGTCGATCTTTGTTA
CACCCGTTTTGATTCGGCGT

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diamino pimelate--D-alanyl-D-alanyl ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 496; Mature: 496

Protein sequence:

>496_residues
MIDLTVEQIARIVGGTLTQGTDPETVVTGPVEFDSRKVSEGSLFVALAGARVDGHDFVPAALESGAAAALVAHPVKIDSG
LAPAIVVPPATGQQQSANSYATEHDEDGSVAAVLQGMGLIARRVIDTLTAQGLTVMGVTGSAGKTSTKDMLASILSNQAP
TVAPPGSFNNEIGHPYTALRCTEQTRYLVAEMSARGIGHIAHLAQIAPPKIGVVLNVGTAHLGEFGSREVIAQAKGELVE
ALPHDGVAVLNADDDLVAGMRTRTDAKVITFSANSQDADVYATDIELDDYARARFTLHIGEAENVVTLRVAGQHQVSNAL
AATAAAYAAGIAIDDIAEALSNHLGASAHRMALMHRSDGATIIDDAYNANTESMRAGLTALSTTAHNKSHKGEASHAWAV
LGPMSELGEESVAAHEEIGRLLGELNVHGLIVVGDSADSLALADGARRSNVTTLMARDTAEAADLLDQNMGSADVALVKA
SNAYRLWETAEILASK

Sequences:

>Translated_496_residues
MIDLTVEQIARIVGGTLTQGTDPETVVTGPVEFDSRKVSEGSLFVALAGARVDGHDFVPAALESGAAAALVAHPVKIDSG
LAPAIVVPPATGQQQSANSYATEHDEDGSVAAVLQGMGLIARRVIDTLTAQGLTVMGVTGSAGKTSTKDMLASILSNQAP
TVAPPGSFNNEIGHPYTALRCTEQTRYLVAEMSARGIGHIAHLAQIAPPKIGVVLNVGTAHLGEFGSREVIAQAKGELVE
ALPHDGVAVLNADDDLVAGMRTRTDAKVITFSANSQDADVYATDIELDDYARARFTLHIGEAENVVTLRVAGQHQVSNAL
AATAAAYAAGIAIDDIAEALSNHLGASAHRMALMHRSDGATIIDDAYNANTESMRAGLTALSTTAHNKSHKGEASHAWAV
LGPMSELGEESVAAHEEIGRLLGELNVHGLIVVGDSADSLALADGARRSNVTTLMARDTAEAADLLDQNMGSADVALVKA
SNAYRLWETAEILASK
>Mature_496_residues
MIDLTVEQIARIVGGTLTQGTDPETVVTGPVEFDSRKVSEGSLFVALAGARVDGHDFVPAALESGAAAALVAHPVKIDSG
LAPAIVVPPATGQQQSANSYATEHDEDGSVAAVLQGMGLIARRVIDTLTAQGLTVMGVTGSAGKTSTKDMLASILSNQAP
TVAPPGSFNNEIGHPYTALRCTEQTRYLVAEMSARGIGHIAHLAQIAPPKIGVVLNVGTAHLGEFGSREVIAQAKGELVE
ALPHDGVAVLNADDDLVAGMRTRTDAKVITFSANSQDADVYATDIELDDYARARFTLHIGEAENVVTLRVAGQHQVSNAL
AATAAAYAAGIAIDDIAEALSNHLGASAHRMALMHRSDGATIIDDAYNANTESMRAGLTALSTTAHNKSHKGEASHAWAV
LGPMSELGEESVAAHEEIGRLLGELNVHGLIVVGDSADSLALADGARRSNVTTLMARDTAEAADLLDQNMGSADVALVKA
SNAYRLWETAEILASK

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=446, Percent_Identity=34.0807174887892, Blast_Score=187, Evalue=1e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 51385; Mature: 51385

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDLTVEQIARIVGGTLTQGTDPETVVTGPVEFDSRKVSEGSLFVALAGARVDGHDFVPA
CCCCCHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCCCCEEEEEECCEECCCCCCHH
ALESGAAAALVAHPVKIDSGLAPAIVVPPATGQQQSANSYATEHDEDGSVAAVLQGMGLI
HHCCCCEEEEEEECEEECCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
ARRVIDTLTAQGLTVMGVTGSAGKTSTKDMLASILSNQAPTVAPPGSFNNEIGHPYTALR
HHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEE
CTEQTRYLVAEMSARGIGHIAHLAQIAPPKIGVVLNVGTAHLGEFGSREVIAQAKGELVE
ECHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEECCCHHHCCCCCHHHHHHHHHHHHH
ALPHDGVAVLNADDDLVAGMRTRTDAKVITFSANSQDADVYATDIELDDYARARFTLHIG
HCCCCCEEEEECCCHHHHCCCCCCCCEEEEEECCCCCCCEEEEECCCCCCCEEEEEEEEC
EAENVVTLRVAGQHQVSNALAATAAAYAAGIAIDDIAEALSNHLGASAHRMALMHRSDGA
CCCCEEEEEECCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHEEEEECCCCC
TIIDDAYNANTESMRAGLTALSTTAHNKSHKGEASHAWAVLGPMSELGEESVAAHEEIGR
EEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCHHHHCHHHHHHHHHHHH
LLGELNVHGLIVVGDSADSLALADGARRSNVTTLMARDTAEAADLLDQNMGSADVALVKA
HHHHCCCCEEEEECCCCCCEEECCCCCCCCCEEEEECCCHHHHHHHHCCCCCCEEEEEEC
SNAYRLWETAEILASK
CCCEEEHHHHHHHHCC
>Mature Secondary Structure
MIDLTVEQIARIVGGTLTQGTDPETVVTGPVEFDSRKVSEGSLFVALAGARVDGHDFVPA
CCCCCHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCCCCEEEEEECCEECCCCCCHH
ALESGAAAALVAHPVKIDSGLAPAIVVPPATGQQQSANSYATEHDEDGSVAAVLQGMGLI
HHCCCCEEEEEEECEEECCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
ARRVIDTLTAQGLTVMGVTGSAGKTSTKDMLASILSNQAPTVAPPGSFNNEIGHPYTALR
HHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEE
CTEQTRYLVAEMSARGIGHIAHLAQIAPPKIGVVLNVGTAHLGEFGSREVIAQAKGELVE
ECHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEECCCHHHCCCCCHHHHHHHHHHHHH
ALPHDGVAVLNADDDLVAGMRTRTDAKVITFSANSQDADVYATDIELDDYARARFTLHIG
HCCCCCEEEEECCCHHHHCCCCCCCCEEEEEECCCCCCCEEEEECCCCCCCEEEEEEEEC
EAENVVTLRVAGQHQVSNALAATAAAYAAGIAIDDIAEALSNHLGASAHRMALMHRSDGA
CCCCEEEEEECCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHEEEEECCCCC
TIIDDAYNANTESMRAGLTALSTTAHNKSHKGEASHAWAVLGPMSELGEESVAAHEEIGR
EEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCHHHHCHHHHHHHHHHHH
LLGELNVHGLIVVGDSADSLALADGARRSNVTTLMARDTAEAADLLDQNMGSADVALVKA
HHHHCCCCEEEEECCCCCCEEECCCCCCCCCEEEEECCCHHHHHHHHCCCCCCEEEEEEC
SNAYRLWETAEILASK
CCCEEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]