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The map label for this gene is murD [H]

Identifier: 38234175

GI number: 38234175

Start: 1630255

End: 1631697

Strand: Reverse

Name: murD [H]

Synonym: DIP1600

Alternate gene names: 38234175

Gene position: 1631697-1630255 (Counterclockwise)

Preceding gene: 38234176

Following gene: 38234174

Centisome position: 65.57

GC content: 52.95

Gene sequence:

>1443_bases
ATGTCTGCCGCCAAAATGACAGTAAGTGTTGAAGAGCTCAAGGTTTTGCTTCCTGAGTTAGCAGGCCGCGTACTCGTTGC
AGGTGCCGGTGTTTCCGGAGTTGGAATCACTCAGCTGCTGCGAGAGATGGGGTGTGCTGTCACCGTTGCAGATTCCAATT
CTGCGCAGTTGGATAAGCTTGCTCAACAAACTGGTTGTCAGACTATTAGCCCAGCTGATGTGGTTTCGGATGGTTTTCAA
GACTACACAGTGGTGGTCACTTCCCCGGGTTGGCGACCTGATTCGCCGCTTCTCGTTGCTGCTCAAAGCGCTGGTCTTGA
GGTGATTGGCGATGTTGAGCTGGTGTACCGACTCGATCGCGCCGAGGTATTTGGCCCGAAGCGAACCTGGATGGTGGTTA
CCGGTACTAACGGCAAAACCACTACAACGGCCATGCTCGCAGAAATCATGCAACATTCTGGTGCGCGTGCTGCTGCCGTC
GGCAACATCGGTGTATCGGTTGCAGATGCTGTGAGGACCCAACCACGTATTGATGTGTTAGTTGCGGAGCTGTCGAGTTT
TCAGCTGCATTGGTCTTCCACTTTGGTCCCTGACGTTGGAATTTTGCTCAATCTTGCTGATGATCACATTGATTGGCACG
GTAGTTTCGCCCAATATGCACAGGATAAGGCGAAAGTATTAGCTGCTCCCACTGCAATTGCGGGCTTTGACAACGGACAT
GTGATGACGGAAACGACACGCATCCAACGTGCCGAAGATGCTGATCCCATCATCGGATTCACCTTAGGCGAGCCAGCTAA
AGGCATGGTAGGCGTGCGCGATGGACAACTTATCGACTGTGCATTTGGCGATAACGTGGTGTTGCGATCTGCTGAAGGAA
TCGAACCTGCAGGTCCGGCAGGGCTTAACGACGCCCTTGCTGCGGCTGCGGCAGCGCGCTCGATGGGAGTGAGTGCCGTA
TGTATTGAAGAAGCCCTGTCTAAGTTTGAAGTCGCAGGACACCGTGGACAATGCGTGGGGCGTCATAGGGAAGTAGTTGC
GATTGATAATTCGAAAGCAACGAACCCACATGCTGCTGATAGCGCACTTGCTGGTTTCTCTTCAGTAGTGTGGGTTGCTG
GTGGGCAGCTCAAGGGAGCAGAAATCGATGAGCTCATTGTTCGACATGCCGGACGAATCAAAGCAGTAGCGTTGTTGGGC
GTTGATCGTGATGTGATCGAAGATTCTGTTCGGACTCATATTCCTGGTATTCCGGTACTGAGCGTTTCTGAGACTGATCC
ACGTCGCGCAATGGATGAGGCGGTAGCGTGGTCGGTATCCCAAGCAGAGGCAGGCGATGCGATCGTATTGGCTCCTGCAG
CAGCTAGTCTTGATATGTACACCGGCATGGGGCAGCGTGGAGACATGTTCGCTACAGCGATTGCACAACATTTACACGAC
TAA

Upstream 100 bases:

>100_bases
ACTGTTGTGATTCGATTCTGGCTGATCGCCGCGGTGGCTGCGCTCATCGGTGCATCCATTTTCTATGGTGAGTGGCTATC
TCTTACAGGAGTATAAAAAT

Downstream 100 bases:

>100_bases
CATTTTTGTCGGTGAGGAGCACCACAATGAGCACCAACACTTCGAATCGTGGCTCTCGGGTTCGTGACCGGATAGCGAGT
TTTTTGGGCGAAATGATGCG

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 480; Mature: 479

Protein sequence:

>480_residues
MSAAKMTVSVEELKVLLPELAGRVLVAGAGVSGVGITQLLREMGCAVTVADSNSAQLDKLAQQTGCQTISPADVVSDGFQ
DYTVVVTSPGWRPDSPLLVAAQSAGLEVIGDVELVYRLDRAEVFGPKRTWMVVTGTNGKTTTTAMLAEIMQHSGARAAAV
GNIGVSVADAVRTQPRIDVLVAELSSFQLHWSSTLVPDVGILLNLADDHIDWHGSFAQYAQDKAKVLAAPTAIAGFDNGH
VMTETTRIQRAEDADPIIGFTLGEPAKGMVGVRDGQLIDCAFGDNVVLRSAEGIEPAGPAGLNDALAAAAAARSMGVSAV
CIEEALSKFEVAGHRGQCVGRHREVVAIDNSKATNPHAADSALAGFSSVVWVAGGQLKGAEIDELIVRHAGRIKAVALLG
VDRDVIEDSVRTHIPGIPVLSVSETDPRRAMDEAVAWSVSQAEAGDAIVLAPAAASLDMYTGMGQRGDMFATAIAQHLHD

Sequences:

>Translated_480_residues
MSAAKMTVSVEELKVLLPELAGRVLVAGAGVSGVGITQLLREMGCAVTVADSNSAQLDKLAQQTGCQTISPADVVSDGFQ
DYTVVVTSPGWRPDSPLLVAAQSAGLEVIGDVELVYRLDRAEVFGPKRTWMVVTGTNGKTTTTAMLAEIMQHSGARAAAV
GNIGVSVADAVRTQPRIDVLVAELSSFQLHWSSTLVPDVGILLNLADDHIDWHGSFAQYAQDKAKVLAAPTAIAGFDNGH
VMTETTRIQRAEDADPIIGFTLGEPAKGMVGVRDGQLIDCAFGDNVVLRSAEGIEPAGPAGLNDALAAAAAARSMGVSAV
CIEEALSKFEVAGHRGQCVGRHREVVAIDNSKATNPHAADSALAGFSSVVWVAGGQLKGAEIDELIVRHAGRIKAVALLG
VDRDVIEDSVRTHIPGIPVLSVSETDPRRAMDEAVAWSVSQAEAGDAIVLAPAAASLDMYTGMGQRGDMFATAIAQHLHD
>Mature_479_residues
SAAKMTVSVEELKVLLPELAGRVLVAGAGVSGVGITQLLREMGCAVTVADSNSAQLDKLAQQTGCQTISPADVVSDGFQD
YTVVVTSPGWRPDSPLLVAAQSAGLEVIGDVELVYRLDRAEVFGPKRTWMVVTGTNGKTTTTAMLAEIMQHSGARAAAVG
NIGVSVADAVRTQPRIDVLVAELSSFQLHWSSTLVPDVGILLNLADDHIDWHGSFAQYAQDKAKVLAAPTAIAGFDNGHV
MTETTRIQRAEDADPIIGFTLGEPAKGMVGVRDGQLIDCAFGDNVVLRSAEGIEPAGPAGLNDALAAAAAARSMGVSAVC
IEEALSKFEVAGHRGQCVGRHREVVAIDNSKATNPHAADSALAGFSSVVWVAGGQLKGAEIDELIVRHAGRIKAVALLGV
DRDVIEDSVRTHIPGIPVLSVSETDPRRAMDEAVAWSVSQAEAGDAIVLAPAAASLDMYTGMGQRGDMFATAIAQHLHD

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=460, Percent_Identity=27.1739130434783, Blast_Score=113, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 50017; Mature: 49886

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAAKMTVSVEELKVLLPELAGRVLVAGAGVSGVGITQLLREMGCAVTVADSNSAQLDKL
CCCCEEEEEHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHCCCEEEEECCCCHHHHHH
AQQTGCQTISPADVVSDGFQDYTVVVTSPGWRPDSPLLVAAQSAGLEVIGDVELVYRLDR
HHHHCCCCCCCHHHHHCCCCCEEEEEECCCCCCCCCEEEEECCCCCEEEECEEEEEEECH
AEVFGPKRTWMVVTGTNGKTTTTAMLAEIMQHSGARAAAVGNIGVSVADAVRTQPRIDVL
HHHCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHCCCCCHHHH
VAELSSFQLHWSSTLVPDVGILLNLADDHIDWHGSFAQYAQDKAKVLAAPTAIAGFDNGH
EEHHHCEEEEECCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHEEECCCEEECCCCCE
VMTETTRIQRAEDADPIIGFTLGEPAKGMVGVRDGQLIDCAFGDNVVLRSAEGIEPAGPA
EEEEHHHHHCCCCCCCEEEEECCCCCCCCEECCCCCEEEEECCCCEEEECCCCCCCCCCC
GLNDALAAAAAARSMGVSAVCIEEALSKFEVAGHRGQCVGRHREVVAIDNSKATNPHAAD
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCHH
SALAGFSSVVWVAGGQLKGAEIDELIVRHAGRIKAVALLGVDRDVIEDSVRTHIPGIPVL
HHHHHHHHEEEEECCCCCCCHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHCCCCCCEE
SVSETDPRRAMDEAVAWSVSQAEAGDAIVLAPAAASLDMYTGMGQRGDMFATAIAQHLHD
ECCCCCHHHHHHHHHHEECCCCCCCCEEEEECCCCCHHHHHCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
SAAKMTVSVEELKVLLPELAGRVLVAGAGVSGVGITQLLREMGCAVTVADSNSAQLDKL
CCCEEEEEHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHCCCEEEEECCCCHHHHHH
AQQTGCQTISPADVVSDGFQDYTVVVTSPGWRPDSPLLVAAQSAGLEVIGDVELVYRLDR
HHHHCCCCCCCHHHHHCCCCCEEEEEECCCCCCCCCEEEEECCCCCEEEECEEEEEEECH
AEVFGPKRTWMVVTGTNGKTTTTAMLAEIMQHSGARAAAVGNIGVSVADAVRTQPRIDVL
HHHCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHCCCCCHHHH
VAELSSFQLHWSSTLVPDVGILLNLADDHIDWHGSFAQYAQDKAKVLAAPTAIAGFDNGH
EEHHHCEEEEECCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHEEECCCEEECCCCCE
VMTETTRIQRAEDADPIIGFTLGEPAKGMVGVRDGQLIDCAFGDNVVLRSAEGIEPAGPA
EEEEHHHHHCCCCCCCEEEEECCCCCCCCEECCCCCEEEEECCCCEEEECCCCCCCCCCC
GLNDALAAAAAARSMGVSAVCIEEALSKFEVAGHRGQCVGRHREVVAIDNSKATNPHAAD
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCHH
SALAGFSSVVWVAGGQLKGAEIDELIVRHAGRIKAVALLGVDRDVIEDSVRTHIPGIPVL
HHHHHHHHEEEEECCCCCCCHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHCCCCCCEE
SVSETDPRRAMDEAVAWSVSQAEAGDAIVLAPAAASLDMYTGMGQRGDMFATAIAQHLHD
ECCCCCHHHHHHHHHHEECCCCCCCCEEEEECCCCCHHHHHCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14602910 [H]